##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936065.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559077
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.787351295081	31.0	31.0	34.0	30.0	34.0
2	31.9170346839523	33.0	31.0	34.0	30.0	34.0
3	31.732074472747044	33.0	31.0	34.0	30.0	34.0
4	35.530785562632694	37.0	35.0	37.0	33.0	37.0
5	30.995367364423863	37.0	35.0	37.0	0.0	37.0
6	33.287699189914804	37.0	35.0	37.0	17.0	37.0
7	29.749059610751292	35.0	32.0	37.0	0.0	37.0
8	32.77157171552398	35.0	35.0	37.0	17.0	37.0
9	36.58268896770928	39.0	35.0	39.0	32.0	39.0
10	36.735113410138496	38.0	35.0	39.0	33.0	39.0
11	36.86823281945063	39.0	37.0	39.0	33.0	39.0
12	36.97656673409924	39.0	37.0	39.0	33.0	39.0
13	36.84639146307217	39.0	37.0	39.0	33.0	39.0
14	38.103483062261546	40.0	37.0	41.0	33.0	41.0
15	38.20805184259056	40.0	37.0	41.0	33.0	41.0
16	38.22991287425525	40.0	37.0	41.0	34.0	41.0
17	38.20635261332518	40.0	37.0	41.0	34.0	41.0
18	38.11976883327341	40.0	37.0	41.0	33.0	41.0
19	38.01298211158749	40.0	37.0	41.0	33.0	41.0
20	37.931199101376016	40.0	37.0	41.0	33.0	41.0
21	37.84263884938926	40.0	36.0	41.0	33.0	41.0
22	37.81536890267351	40.0	36.0	41.0	33.0	41.0
23	37.759251409018795	39.0	36.0	41.0	33.0	41.0
24	37.7261647322283	39.0	36.0	41.0	33.0	41.0
25	37.616330129839	39.0	36.0	41.0	33.0	41.0
26	37.46740073370931	39.0	36.0	41.0	33.0	41.0
27	37.43738161290842	39.0	36.0	41.0	33.0	41.0
28	37.34810052998066	39.0	36.0	41.0	32.0	41.0
29	37.35078888954473	39.0	36.0	41.0	32.0	41.0
30	37.21397231508361	39.0	36.0	41.0	32.0	41.0
31	37.085684082872305	39.0	35.0	41.0	31.0	41.0
32	36.9843348948356	39.0	35.0	41.0	31.0	41.0
33	36.76355314205378	39.0	35.0	41.0	30.0	41.0
34	36.60204229471075	39.0	35.0	41.0	30.0	41.0
35	36.46191848350048	39.0	35.0	41.0	30.0	41.0
36	36.290958132779565	39.0	35.0	40.0	30.0	41.0
37	36.160832944299266	39.0	35.0	40.0	29.0	41.0
38	36.08126250945755	39.0	35.0	40.0	29.0	41.0
39	36.05396752146842	39.0	35.0	40.0	29.0	41.0
40	35.922481160913435	39.0	35.0	40.0	28.0	41.0
41	35.8344181570696	39.0	35.0	40.0	27.0	41.0
42	35.7600759823781	39.0	35.0	40.0	27.0	41.0
43	35.55077565344308	39.0	35.0	40.0	26.0	41.0
44	35.39330718308927	38.0	35.0	40.0	26.0	41.0
45	35.25625808251815	38.0	34.0	40.0	25.0	41.0
46	35.13854442232465	38.0	34.0	40.0	24.0	41.0
47	34.959239961579534	38.0	34.0	40.0	23.0	41.0
48	34.85565315689968	38.0	34.0	40.0	23.0	41.0
49	34.665915428465134	38.0	34.0	40.0	23.0	41.0
50	34.48140953750557	38.0	34.0	40.0	22.0	41.0
51	32.55994791415136	36.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	16.0
10	22.0
11	21.0
12	12.0
13	10.0
14	8.0
15	28.0
16	46.0
17	83.0
18	184.0
19	369.0
20	663.0
21	1162.0
22	1801.0
23	2745.0
24	4134.0
25	5879.0
26	7028.0
27	7878.0
28	8839.0
29	9939.0
30	12179.0
31	15092.0
32	19076.0
33	25831.0
34	36634.0
35	46203.0
36	53957.0
37	85782.0
38	108451.0
39	104979.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.830470579186766	21.231601371546315	27.17139141835561	11.766536630911306
2	32.01347935973041	24.148194255889617	27.05101443987143	16.787311944508538
3	31.676674232708553	23.790998377683216	28.399487011628093	16.132840377980134
4	26.565929201165495	27.080885101694403	28.64954201299642	17.703643684143685
5	21.434077953484046	39.80006331864841	23.05138648164743	15.71447224622011
6	24.94075055850983	35.07745802456549	26.44877181497361	13.533019601951073
7	70.88057637856681	18.495663388048516	7.137478379543426	3.4862818538412417
8	85.72128704990547	4.068312593792984	7.386460183480987	2.8239401728205595
9	80.04854429711828	7.199366098050895	8.925246432960039	3.82684317187078
10	36.996335030773935	33.91518520704662	17.108019110068916	11.980460652110532
11	26.015021186705944	26.73388459908027	29.981737757053146	17.269356457160644
12	24.796584370310352	24.184146369820258	32.43935987350579	18.579909386363596
13	22.270456484527177	26.903092060664274	32.72804998238168	18.098401472426875
14	18.95946354437761	29.245882052025035	31.803669262015788	19.99098514158157
15	17.93921052019668	29.52741751136248	35.116271998311504	17.41709997012934
16	22.329661209457733	26.893075551310464	32.659544213051156	18.11771902618065
17	21.927927637874568	27.110040298563526	29.92360622955335	21.038425834008553
18	22.185673887496712	27.550051245177322	31.176921962448823	19.087352904877143
19	22.003051458028143	28.932508402241552	30.269175802259795	18.79526433747051
20	23.571708369330164	28.00687561820644	30.019478533368392	18.40193747909501
21	22.644823521625824	27.79778098544565	31.096253288902957	18.461142204025563
22	21.87784509110552	25.93542571059085	30.650160890181493	21.536568308122135
23	19.92480463335104	27.67919266934608	31.17441783511037	21.221584862192508
24	20.204730296542337	27.358664370024165	31.941753998107597	20.494851335325905
25	19.963082008381672	29.082934908787163	30.521198332251192	20.432784750579973
26	19.53147777497554	31.061195506164623	29.73436574926173	19.672960969598105
27	19.887958188228097	29.683925470015758	31.070854283041516	19.357262058714632
28	18.401222014141165	28.496969111589284	32.91371313790408	20.188095736365476
29	20.001001650935383	26.969093702656345	32.189841470852855	20.84006317555542
30	20.50146938614896	28.73182048268843	30.82688073378086	19.939829397381757
31	21.143241449746636	28.519863990112277	29.66371358506968	20.67318097507141
32	21.388288196438058	28.754536494972964	29.939346458537912	19.917828850051066
33	20.701441840748235	29.706820348538756	29.637599114254385	19.954138696458628
34	19.943048989674054	27.464374316954554	30.738163079504254	21.85441361386714
35	19.58925156999841	27.799748514068725	30.216052529436908	22.394947386495957
36	21.043076356208537	29.492359728624145	29.380389463347623	20.084174451819695
37	19.901552022351122	29.197767033879053	31.079082130010715	19.821598813759106
38	19.478712234629576	28.88922277253402	30.011429552637654	21.620635440198757
39	20.677652631032934	27.64485035156159	30.0656260228913	21.611870994514174
40	21.00175825512407	27.36152622983954	29.298826458609454	22.33788905642693
41	19.408775535391367	27.635907039638546	29.390763705178358	23.56455371979173
42	21.390792323776513	27.7582515467458	28.88636091271864	21.96459521675905
43	21.23410549888477	27.914401772922155	29.791602945569217	21.059889782623863
44	20.25517057578831	28.43543912555873	29.789098818230762	21.520291480422195
45	19.96648046691243	28.47300103563552	29.51042521870869	22.050093278743358
46	20.521323538618116	28.781724163219017	28.41522724061265	22.28172505755021
47	19.95664282379708	27.493708380062138	30.07546366600665	22.47418513013413
48	20.346750089880285	26.858375501049053	30.43033428311306	22.364540125957603
49	20.7386460183481	27.1211300053481	30.194230848344684	21.945993127959117
50	19.5069731003064	27.054055165925266	29.61631403187754	23.822657701890794
51	18.805817445539702	27.241149251355356	28.518790792681507	25.43424251042343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	489.5
2	677.0
3	2804.5
4	4932.0
5	3898.5
6	2865.0
7	2340.5
8	1816.0
9	1778.5
10	1741.0
11	1753.5
12	1766.0
13	1736.0
14	1706.0
15	1698.5
16	1691.0
17	1728.0
18	1765.0
19	1973.5
20	2182.0
21	2445.5
22	2709.0
23	3293.0
24	3877.0
25	4695.5
26	6537.0
27	7560.0
28	8934.0
29	10308.0
30	12615.5
31	14923.0
32	16700.5
33	18478.0
34	20428.5
35	22379.0
36	24142.5
37	25906.0
38	28088.0
39	30270.0
40	32625.5
41	34981.0
42	37501.0
43	40021.0
44	42561.0
45	45101.0
46	47722.0
47	50343.0
48	51414.0
49	52485.0
50	49018.5
51	45552.0
52	40080.5
53	34609.0
54	29748.5
55	24888.0
56	21995.5
57	19103.0
58	17309.0
59	15515.0
60	13827.0
61	12139.0
62	10356.5
63	8574.0
64	7079.0
65	5584.0
66	4175.5
67	2767.0
68	2199.0
69	1631.0
70	1342.5
71	1054.0
72	820.0
73	586.0
74	479.5
75	285.0
76	197.0
77	142.0
78	87.0
79	67.0
80	47.0
81	37.0
82	27.0
83	20.5
84	14.0
85	15.0
86	16.0
87	12.0
88	8.0
89	7.5
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.03826073002968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83309067136578	33.06762589008251
2	8.627117061284023	7.4259222686405835
3	3.5731355329434535	4.6134461607166175
4	2.099877277606531	3.615002630987792
5	1.4797878052916351	3.1843746694629886
6	1.0990250986209826	2.838007724597786
7	0.8052739952276974	2.426031451600562
8	0.6290500962699311	2.165857764441245
9	0.5031281257204576	1.9488383509831384
>10	4.288597318537019	36.0179309737519
>50	0.049106599797921376	1.307222591124387
>100	0.011956389512258452	0.8512659975355348
>500	4.2701391115208755E-4	0.13397515375721175
>1k	4.2701391115208755E-4	0.4044983723177271
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2201	0.39368459085242286	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.13039348783799012	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09873416363041226	0.0
2	0.0	0.0	0.0	0.41479080699080806	0.0
3	0.0	0.0	0.0	0.5804209438055938	0.0
4	0.0	0.0	0.0	0.8081176653663091	0.0
5	0.0	0.0	0.0	1.42001906714102	0.0
6	0.0	0.0	0.0	1.8838192234701123	0.0
7	0.0	0.0	0.0	2.3497657746607357	0.0
8	0.0	0.0	0.0	3.132305567927137	0.0
9	0.0	0.0	0.0	3.46785863127977	0.0
10	0.0	0.0	0.0	4.268821647107644	0.0
11	0.0	0.0	0.0	5.293725193488553	0.0
12	0.0	0.0	0.0	6.122591342516326	0.0
13	0.0	0.0	0.0	6.473348036138135	0.0
14	0.0	0.0	0.0	6.627351867452963	0.0
15	0.0	0.0	0.0	6.891716167898161	0.0
16	0.0	0.0	0.0	7.498430448757506	0.0
17	0.0	0.0	0.0	8.288661490277725	0.0
18	0.0	0.0	0.0	9.088193576197911	0.0
19	0.0	0.0	0.0	9.584547387926886	0.0
20	0.0	0.0	0.0	10.069274894155903	0.0
21	0.0	0.0	0.0	10.655061825115324	0.0
22	0.0	0.0	0.0	11.300947812197604	0.0
23	0.0	0.0	0.0	11.966687951749044	0.0
24	0.0	0.0	0.0	12.48933510053177	0.0
25	0.0	0.0	0.0	12.9447285436532	0.0
26	0.0	0.0	0.0	13.36005594935939	0.0
27	0.0	0.0	0.0	13.79899369854242	0.0
28	0.0	0.0	0.0	14.240972173779282	0.0
29	0.0	0.0	0.0	14.73768371798518	0.0
30	0.0	0.0	0.0	15.268558713737105	0.0
31	0.0	0.0	0.0	15.791921327473675	0.0
32	0.0	0.0	0.0	16.28773854048727	0.0
33	0.0	0.0	0.0	16.764774798462465	0.0
34	0.0	0.0	0.0	17.235371871853072	0.0
35	0.0	0.0	0.0	17.73208341605897	0.0
36	0.0	0.0	0.0	18.22968929145717	0.0
37	0.0	0.0	0.0	18.730157026670746	0.0
38	1.7886623846089178E-4	0.0	0.0	19.215599997853605	0.0
39	1.7886623846089178E-4	0.0	0.0	19.725368777467146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	20	7.030173E-4	45.000004	2
GCCGATC	25	3.8882255E-5	45.000004	9
GAGCGTA	25	3.8882255E-5	45.000004	8
AATTGCG	30	2.1634933E-6	45.000004	1
TACTCGC	20	7.030173E-4	45.000004	45
TTCGGTG	30	2.1634933E-6	45.000004	21
TCGAGTC	20	7.030173E-4	45.000004	29
CGCACGG	20	7.030173E-4	45.000004	2
TGCAACG	45	3.8380676E-10	45.000004	1
AAGCGCC	35	1.2104283E-7	45.0	14
TACGCGG	35	1.2104283E-7	45.0	2
TGCGACG	35	1.2104283E-7	45.0	1
CGATGAT	35	1.2104283E-7	45.0	10
CGCCCCG	35	1.2104283E-7	45.0	17
CGACGGG	60	3.6379788E-12	41.250004	3
CGTTTTT	1005	0.0	40.97015	1
TTGTGCG	55	6.002665E-11	40.909092	1
GCGGCTA	50	1.0786607E-9	40.500004	24
CGGCTAC	50	1.0786607E-9	40.500004	25
GGCCGAT	95	0.0	40.263157	8
>>END_MODULE