##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936064.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628084
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94545474809102	31.0	31.0	34.0	30.0	34.0
2	32.08906452003235	33.0	31.0	34.0	30.0	34.0
3	32.0024248348947	33.0	31.0	34.0	30.0	34.0
4	35.73139739270543	37.0	35.0	37.0	35.0	37.0
5	31.2183449984397	37.0	35.0	37.0	0.0	37.0
6	33.43075926150006	37.0	35.0	37.0	17.0	37.0
7	29.538270358741823	35.0	32.0	37.0	0.0	37.0
8	32.63088058285197	35.0	33.0	37.0	17.0	37.0
9	36.516537596881946	39.0	35.0	39.0	32.0	39.0
10	36.87766763681291	38.0	37.0	39.0	33.0	39.0
11	36.97391750148069	39.0	37.0	39.0	33.0	39.0
12	36.89124225422077	39.0	35.0	39.0	33.0	39.0
13	36.64685456085492	39.0	35.0	39.0	33.0	39.0
14	37.674140720031076	40.0	37.0	41.0	33.0	41.0
15	37.843946032696266	40.0	37.0	41.0	33.0	41.0
16	37.95269263346941	40.0	37.0	41.0	33.0	41.0
17	37.95154947427414	40.0	36.0	41.0	33.0	41.0
18	37.88086466141471	40.0	36.0	41.0	33.0	41.0
19	37.813236764509206	40.0	36.0	41.0	33.0	41.0
20	37.71773520739264	40.0	36.0	41.0	33.0	41.0
21	37.61423949662784	40.0	36.0	41.0	33.0	41.0
22	37.663156520465414	39.0	36.0	41.0	33.0	41.0
23	37.62740174881067	39.0	35.0	41.0	33.0	41.0
24	37.568962113347894	39.0	35.0	41.0	33.0	41.0
25	37.439401417644774	39.0	35.0	41.0	33.0	41.0
26	37.28647919705008	39.0	35.0	41.0	32.0	41.0
27	37.24540188891932	39.0	35.0	41.0	32.0	41.0
28	37.14628775768846	39.0	35.0	41.0	32.0	41.0
29	37.08902153215175	39.0	35.0	41.0	32.0	41.0
30	36.936449583176774	39.0	35.0	41.0	31.0	41.0
31	36.77853758414479	39.0	35.0	41.0	31.0	41.0
32	36.66677068672343	39.0	35.0	41.0	31.0	41.0
33	36.490695512065265	39.0	35.0	40.0	30.0	41.0
34	36.30791104374574	39.0	35.0	40.0	30.0	41.0
35	36.14677336152489	39.0	35.0	40.0	29.0	41.0
36	36.0319304424249	39.0	35.0	40.0	29.0	41.0
37	35.91789951662516	38.0	35.0	40.0	29.0	41.0
38	35.70009425490858	38.0	35.0	40.0	27.0	41.0
39	35.633357958489626	38.0	35.0	40.0	27.0	41.0
40	35.45733850886187	38.0	34.0	40.0	26.0	41.0
41	35.303556530655136	38.0	34.0	40.0	26.0	41.0
42	35.26237254889473	38.0	34.0	40.0	26.0	41.0
43	35.13396934168041	38.0	34.0	40.0	25.0	41.0
44	34.96200826641022	38.0	34.0	40.0	24.0	41.0
45	34.82706134848205	38.0	34.0	40.0	24.0	41.0
46	34.72035587596564	38.0	34.0	40.0	23.0	41.0
47	34.502947058036824	37.0	33.0	40.0	23.0	41.0
48	34.39871896115806	37.0	33.0	40.0	23.0	41.0
49	34.27390126161469	37.0	33.0	40.0	23.0	41.0
50	34.067989313531314	37.0	33.0	40.0	22.0	41.0
51	32.131106667261065	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	28.0
10	30.0
11	35.0
12	24.0
13	23.0
14	32.0
15	50.0
16	92.0
17	206.0
18	384.0
19	646.0
20	1028.0
21	1615.0
22	2447.0
23	3520.0
24	4830.0
25	6280.0
26	7839.0
27	9380.0
28	10362.0
29	12003.0
30	14444.0
31	18327.0
32	23288.0
33	33124.0
34	47491.0
35	53481.0
36	61327.0
37	94165.0
38	114611.0
39	106951.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18469504079072	20.737831245502196	23.90349061590488	11.173983097802205
2	33.81856566956012	23.734086523458643	25.26556957349654	17.181778233484692
3	33.59025225925195	24.011437960527573	25.810082727788004	16.588227052432476
4	28.93052521637233	27.06357748326657	25.088045548047717	18.917851752313386
5	22.02205437489253	40.08078537265716	21.726234070602022	16.170926181848287
6	26.70630043115252	34.29796014545825	25.224810694110978	13.77092872927825
7	70.125174339738	19.73398462626018	6.385610841861916	3.755230192139905
8	84.4380687933461	4.885174594480993	7.1499990447137645	3.5267575674591294
9	79.14753440622592	6.760242260589348	9.122505906853224	4.96971742633151
10	44.24631100298686	25.733659828940077	16.299093751791162	13.720935416281899
11	33.25144407435948	25.320020888925686	24.060953630406125	17.36758140630871
12	30.485731207927603	23.69794486087848	27.1315620203667	18.684761910827213
13	26.964864572254665	24.56263811846823	29.78709854095949	18.68539876831761
14	22.350513625566006	26.043968641137173	31.013845281841284	20.591672451455537
15	21.481362365543465	25.36746677196044	33.77653307519377	19.374637787302333
16	27.021544888900213	24.840148769909757	29.60320594060667	18.535100400583364
17	27.16579311047567	24.160462613281027	27.41846631979162	21.255277956451685
18	28.152922220594697	24.066366919074518	28.554142439546304	19.22656842078448
19	26.761707032817267	26.623349743028	26.53355283688169	20.08139038727304
20	26.37067653371205	28.483132829366774	26.61331923755421	18.53287139936696
21	26.200954012520615	25.902586278268515	29.118716604785348	18.77774310442552
22	24.72503677852007	23.79092605447679	29.630909241439042	21.8531279255641
23	23.38317804624859	25.350271619719656	30.02846752982085	21.2380828042109
24	23.89139032358729	25.63080734424058	29.276338833659192	21.201463498512936
25	24.99140242387961	25.470000827914735	28.014883359550634	21.523713388655022
26	22.637736353736123	26.556002063418273	27.29077639296655	23.51548518987906
27	22.13414129320282	25.60007897032881	30.20025983785608	22.065519898612287
28	21.057374491310078	26.914075187395316	28.336496392202314	23.69205392909229
29	22.99756083581177	27.52147801886372	28.134930996490915	21.346030148833595
30	25.41825615681979	25.074512326376723	28.514020417651142	20.993211099152344
31	25.55135937231326	25.918348501155897	27.31545462071952	21.214837505811325
32	27.173435400360464	26.183918074652436	26.06689551079155	20.575751014195554
33	26.812974060794414	25.815496016456397	26.40188255074162	20.969647372007564
34	23.651772692824526	26.217512307271	29.299106488940968	20.831608510963502
35	24.174791906815013	27.624967361053614	28.13015456531292	20.070086166818452
36	26.17914164347444	27.879869571585964	25.991268683806624	19.94972010113297
37	24.52410824029907	28.67880729329198	25.827755523146585	20.969328943262365
38	24.85813999401354	28.540927646620517	25.5085307060839	21.092401653282046
39	24.486215219620306	26.480693665178546	26.722221868412504	22.310869246788645
40	25.881569981085335	25.724425395329288	27.295552824144544	21.09845179944084
41	22.477566694900684	26.509511466619113	27.02313703262621	23.989784805853994
42	23.09261181625388	26.43133720967259	27.829876258589614	22.646174715483916
43	22.90250985536966	25.430993306627776	28.36611026550589	23.300386572496674
44	23.317263295992255	26.027728775131987	27.90661758618274	22.748390342693014
45	23.985645232166398	26.020564128365	26.150323842033867	23.843466797434736
46	23.460397016959515	25.937295011495276	27.213875851000825	23.388432120544387
47	22.259761433184096	27.139363524624095	28.574521879239086	22.026353162952724
48	22.85570082982531	27.108157507594527	28.17139108781628	21.864750574763885
49	24.475229427910918	25.782060998210433	27.90661758618274	21.836091987695912
50	22.637099496245725	26.23884703319938	27.941326319409505	23.18272715114539
51	22.487278771629274	26.624941886754	26.91327911553232	23.97450022608441
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	308.0
1	799.5
2	1291.0
3	2224.0
4	3157.0
5	2424.5
6	1692.0
7	1484.0
8	1276.0
9	1263.0
10	1250.0
11	1249.0
12	1248.0
13	1230.0
14	1212.0
15	1264.0
16	1316.0
17	1364.5
18	1413.0
19	1518.0
20	1623.0
21	1924.5
22	2226.0
23	2504.5
24	2783.0
25	3593.5
26	4989.5
27	5575.0
28	6669.0
29	7763.0
30	9422.0
31	11081.0
32	12720.0
33	14359.0
34	16039.5
35	17720.0
36	19672.0
37	21624.0
38	23468.5
39	25313.0
40	28139.0
41	30965.0
42	32762.5
43	34560.0
44	37770.5
45	40981.0
46	42968.5
47	44956.0
48	48120.5
49	51285.0
50	50759.0
51	50233.0
52	47174.0
53	44115.0
54	40672.0
55	37229.0
56	35683.0
57	34137.0
58	33430.0
59	32723.0
60	29518.5
61	26314.0
62	24317.0
63	22320.0
64	19531.5
65	16743.0
66	14102.5
67	11462.0
68	9670.5
69	7879.0
70	6606.5
71	5334.0
72	4397.0
73	3460.0
74	2729.0
75	1587.5
76	1177.0
77	950.5
78	724.0
79	545.5
80	367.0
81	306.5
82	246.0
83	183.0
84	120.0
85	91.5
86	63.0
87	43.5
88	24.0
89	21.5
90	19.0
91	15.5
92	12.0
93	7.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	628084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.67467981710714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15329832952554	27.562231534348825
2	8.125316554184698	5.959867660747283
3	3.547791602435789	3.9034236323146203
4	2.230177172562679	3.2716413495663024
5	1.566091001137684	2.871794301558867
6	1.2442104212698752	2.7378611295108404
7	0.9548579273368086	2.4513376129132807
8	0.7512673211367561	2.204199076779707
9	0.6360123195018971	2.0992993359720953
>10	5.674707265942644	43.51415224962631
>50	0.09851892696003633	2.184491021154708
>100	0.017307379055465963	1.036420713691664
>500	0.0	0.0
>1k	4.437789501401529E-4	0.20328038181546618
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	0.19885875137720432	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5921437259984334E-4	0.0	0.0	0.06495946402073607	0.0
2	1.5921437259984334E-4	0.0	0.0	0.2689130753211354	0.0
3	1.5921437259984334E-4	0.0	0.0	0.37606434808082995	0.0
4	1.5921437259984334E-4	0.0	0.0	0.5164914247138918	0.0
5	1.5921437259984334E-4	0.0	0.0	0.9681825997796473	0.0
6	1.5921437259984334E-4	0.0	0.0	1.330236083071691	0.0
7	1.5921437259984334E-4	0.0	0.0	1.6744575566325524	0.0
8	1.5921437259984334E-4	0.0	0.0	2.299533183459537	0.0
9	1.5921437259984334E-4	0.0	0.0	2.594875844632247	0.0
10	1.5921437259984334E-4	0.0	0.0	3.1745753752682764	0.0
11	1.5921437259984334E-4	0.0	0.0	3.9230421408601397	0.0
12	1.5921437259984334E-4	0.0	0.0	4.468669795759803	0.0
13	1.5921437259984334E-4	0.0	0.0	4.718158717623758	0.0
14	1.5921437259984334E-4	0.0	0.0	4.843142000114634	0.0
15	1.5921437259984334E-4	0.0	0.0	5.05043911323963	0.0
16	1.5921437259984334E-4	0.0	0.0	5.433827322460053	0.0
17	1.5921437259984334E-4	0.0	0.0	5.891091000566803	0.0
18	1.5921437259984334E-4	0.0	0.0	6.399303277905503	0.0
19	1.5921437259984334E-4	0.0	0.0	6.702925086453404	0.0
20	1.5921437259984334E-4	0.0	0.0	7.028996121537883	0.0
21	1.5921437259984334E-4	0.0	0.0	7.409200043306309	0.0
22	1.5921437259984334E-4	0.0	0.0	7.811693977238713	0.0
23	1.5921437259984334E-4	0.0	0.0	8.216098483642316	0.0
24	1.5921437259984334E-4	0.0	0.0	8.54630909241439	0.0
25	1.5921437259984334E-4	0.0	0.0	8.87253934187147	0.0
26	1.5921437259984334E-4	0.0	0.0	9.157055425707389	0.0
27	1.5921437259984334E-4	0.0	0.0	9.507645474172245	0.0
28	1.5921437259984334E-4	0.0	0.0	9.822889931919935	0.0
29	1.5921437259984334E-4	0.0	0.0	10.164086332401398	0.0
30	1.5921437259984334E-4	0.0	0.0	10.573108055610396	0.0
31	1.5921437259984334E-4	0.0	0.0	10.912871526738462	0.0
32	1.5921437259984334E-4	0.0	0.0	11.261073359614318	0.0
33	1.5921437259984334E-4	0.0	0.0	11.586825965953599	0.0
34	1.5921437259984334E-4	0.0	0.0	11.944262232440247	0.0
35	1.5921437259984334E-4	0.0	0.0	12.31777915055948	0.0
36	1.5921437259984334E-4	0.0	0.0	12.645601543742556	0.0
37	1.5921437259984334E-4	0.0	0.0	13.001445666503207	0.0
38	1.5921437259984334E-4	0.0	0.0	13.402665885454812	0.0
39	1.5921437259984334E-4	0.0	0.0	14.093337833792932	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	20	7.0308393E-4	45.000004	9
TCATCGA	20	7.0308393E-4	45.000004	16
CGGTCTA	20	7.0308393E-4	45.000004	31
CGCACTC	20	7.0308393E-4	45.000004	40
TATTGCG	25	3.888778E-5	45.0	1
CACGACC	105	0.0	42.857143	27
ACCGTAA	40	3.4548793E-7	39.375004	27
AGACACG	115	0.0	39.130436	24
TAGACGG	75	0.0	39.0	2
ACGGGTG	70	0.0	38.571426	5
ACGGGTC	35	6.243765E-6	38.571426	5
TCGGCAC	35	6.243765E-6	38.571426	27
CGCTATC	35	6.243765E-6	38.571426	40
TTACGGA	35	6.243765E-6	38.571426	14
TACGTTG	35	6.243765E-6	38.571426	1
TGTACGG	100	0.0	38.25	2
CGGGTAT	65	9.094947E-12	38.076927	6
ATGCACG	65	9.094947E-12	38.076927	1
TCGCATC	30	1.1393325E-4	37.499996	10
GTCGAAA	30	1.1393325E-4	37.499996	9
>>END_MODULE