##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936063.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468437
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.768549452754588	31.0	31.0	34.0	30.0	34.0
2	31.866468703368863	33.0	31.0	34.0	30.0	34.0
3	31.780551066632224	33.0	31.0	34.0	30.0	34.0
4	35.54452359655621	37.0	35.0	37.0	33.0	37.0
5	31.07204170464758	37.0	35.0	37.0	0.0	37.0
6	33.30983035071952	37.0	35.0	37.0	17.0	37.0
7	29.677173237809992	35.0	32.0	37.0	0.0	37.0
8	32.63143389612691	35.0	33.0	37.0	17.0	37.0
9	36.44258032563611	39.0	35.0	39.0	32.0	39.0
10	36.70770669268226	38.0	35.0	39.0	33.0	39.0
11	36.828570330695484	39.0	37.0	39.0	33.0	39.0
12	36.91443886798011	39.0	37.0	39.0	33.0	39.0
13	36.76167766423233	39.0	35.0	39.0	33.0	39.0
14	38.02626180254762	40.0	37.0	41.0	33.0	41.0
15	38.06683289321723	40.0	37.0	41.0	33.0	41.0
16	38.13339680682781	40.0	37.0	41.0	33.0	41.0
17	38.07791015654186	40.0	37.0	41.0	33.0	41.0
18	38.01471275753196	40.0	37.0	41.0	33.0	41.0
19	37.908448734835204	40.0	37.0	41.0	33.0	41.0
20	37.876154103967025	40.0	36.0	41.0	33.0	41.0
21	37.79354107382636	40.0	36.0	41.0	33.0	41.0
22	37.83651163336799	40.0	36.0	41.0	33.0	41.0
23	37.799958158727854	39.0	36.0	41.0	33.0	41.0
24	37.74847631591868	40.0	36.0	41.0	33.0	41.0
25	37.653667408851135	39.0	36.0	41.0	33.0	41.0
26	37.478025860467895	39.0	36.0	41.0	33.0	41.0
27	37.44651682083183	39.0	36.0	41.0	33.0	41.0
28	37.368800927339215	39.0	36.0	41.0	32.0	41.0
29	37.362968766344245	39.0	36.0	41.0	32.0	41.0
30	37.255812414476225	39.0	36.0	41.0	32.0	41.0
31	37.15751531155737	39.0	36.0	41.0	32.0	41.0
32	37.03563766312226	39.0	35.0	41.0	31.0	41.0
33	36.86519638713423	39.0	35.0	41.0	31.0	41.0
34	36.75879360511659	39.0	35.0	41.0	30.0	41.0
35	36.64920362823603	39.0	35.0	41.0	30.0	41.0
36	36.522563332956196	39.0	35.0	41.0	30.0	41.0
37	36.340216507235766	39.0	35.0	40.0	30.0	41.0
38	36.32003236294315	39.0	35.0	40.0	30.0	41.0
39	36.299348685095325	39.0	35.0	40.0	30.0	41.0
40	36.21906254202806	39.0	35.0	40.0	29.0	41.0
41	36.11071286000038	39.0	35.0	40.0	29.0	41.0
42	36.040195373123815	39.0	35.0	40.0	28.0	41.0
43	35.88033823118157	39.0	35.0	40.0	28.0	41.0
44	35.699077997681655	38.0	35.0	40.0	27.0	41.0
45	35.61402066873454	38.0	35.0	40.0	27.0	41.0
46	35.4837662268352	38.0	34.0	40.0	26.0	41.0
47	35.327747381184665	38.0	34.0	40.0	26.0	41.0
48	35.273311032219915	38.0	34.0	40.0	26.0	41.0
49	35.1064710942987	38.0	34.0	40.0	26.0	41.0
50	34.95230308451296	38.0	34.0	40.0	24.0	41.0
51	32.935654100764886	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	14.0
10	19.0
11	14.0
12	5.0
13	4.0
14	9.0
15	22.0
16	37.0
17	97.0
18	141.0
19	274.0
20	531.0
21	868.0
22	1320.0
23	1957.0
24	2849.0
25	3913.0
26	5015.0
27	5741.0
28	6870.0
29	7991.0
30	10047.0
31	12719.0
32	16461.0
33	23056.0
34	31766.0
35	39977.0
36	45883.0
37	71644.0
38	90788.0
39	88385.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59628082324838	21.60141064860376	26.46609896314766	11.336209565000203
2	28.22087068271721	26.42852720856807	28.465300563362845	16.885301545351883
3	32.59264319428226	25.703776601762883	26.43194282262076	15.271637381334097
4	26.19541154947197	27.982418126663777	27.938015143978806	17.88415517988545
5	21.360609857889106	40.61592060405135	22.226681496124346	15.7967880419352
6	23.651846459609295	36.560092392360126	26.48296355753282	13.30509759049776
7	70.8744185450765	18.505369985718463	7.227866287248872	3.392345181956165
8	84.24612060960172	5.861193714416239	6.644436711873741	3.2482489641083
9	78.93569466118176	7.45607200114423	8.755926624071114	4.852306713602896
10	36.28876455104955	36.39187340026513	15.575627032023517	11.743735016661793
11	25.972329256655648	25.937746164372154	29.4799514128901	18.609973166082096
12	24.622307802329875	24.68250800000854	31.6029690225153	19.092215175146286
13	22.441224753808946	26.615318602074563	33.35261732100581	17.590839323110686
14	19.81504449904683	29.64945126025485	30.389999082053727	20.1455051586446
15	17.93667024594556	28.50415317321219	36.66618990387181	16.892986676970438
16	21.15204392479672	27.2512632435098	31.97100143669266	19.62569139500082
17	20.757113549954422	26.371742624942097	31.252868582114562	21.61827524298892
18	20.993644823103214	28.004619618006267	31.205263461255196	19.796472097635327
19	21.34246440823419	29.73398770805893	29.659698102412918	19.263849781293963
20	23.16533493297925	28.46764879802407	30.36331459726708	18.0037016717296
21	21.65670090108168	29.923981239739817	29.90028541724928	18.519032441929223
22	20.505212013568528	26.944498406402566	30.461940453038512	22.088349126990394
23	19.049946951244245	29.3804716536055	30.743942088263736	20.82563930688652
24	20.713564470782625	26.60336395289014	31.610227202377267	21.072844373949966
25	19.71748602266687	30.96851871222811	28.96547454620365	20.348520718901366
26	20.183290389102485	29.813400734783972	28.92576803284113	21.077540843272413
27	20.250321814886526	30.662180826877467	29.777109835474143	19.310387522761864
28	17.607276965739256	28.917655949465992	32.94423796583105	20.5308291189637
29	20.77397814433958	27.17377149968939	30.92945262650047	21.12279772947056
30	20.790629262846444	29.34140556787786	30.036696503478588	19.831268665797108
31	21.94254510211619	28.22321891737843	27.477120722743937	22.357115257761446
32	21.175099319652375	31.002888328633304	27.787087698025566	20.03492465368876
33	19.73669885171325	30.672854620792123	27.428021270736515	22.162425256758112
34	20.500515544246078	28.603846408375084	28.490490717001432	22.405147330377403
35	19.04140791611252	29.025034316247435	28.075493609599583	23.858064158040463
36	21.528188422349217	29.980125395730912	27.885500077918696	20.60618610400118
37	18.77499002000269	30.546049949086	29.216735654954668	21.46222437595664
38	18.267344381421623	30.806490520603624	26.921869963303497	24.004295134671256
39	19.680554695722158	30.217510572392875	28.28171130803075	21.82022342385422
40	19.95209601291102	28.629463513770258	29.15397374673649	22.26446672658223
41	18.83625759707282	28.768222834660794	27.950396744919807	24.445122823346576
42	19.85987443348839	28.56755550906525	28.761818558312	22.810751499134355
43	20.84549256356778	29.045528000563575	27.65729436402334	22.451685071845308
44	19.282208706827173	29.04382019353723	28.892252319949108	22.78171877968649
45	19.91708596887095	28.224499772648187	28.059055966971012	23.79935829150985
46	21.284185493460168	29.31023808964706	27.419268759726496	21.98630765716628
47	18.9984992645756	27.87973622920478	30.438458106426264	22.683306399793356
48	19.79305648358264	28.480243874843364	28.425167098243737	23.301532543330268
49	19.426091448796743	26.64755345969682	31.2238358626667	22.702519228839737
50	19.119326611689512	27.46730083234245	29.009450577132036	24.403921978836003
51	18.403328515894344	26.598240531811108	28.234746614806262	26.763684337488286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	381.0
2	591.0
3	1707.0
4	2823.0
5	2176.5
6	1530.0
7	1291.0
8	1052.0
9	1057.0
10	1062.0
11	1103.5
12	1145.0
13	1138.0
14	1131.0
15	1136.5
16	1142.0
17	1182.0
18	1222.0
19	1448.0
20	1674.0
21	1998.0
22	2322.0
23	2678.5
24	3035.0
25	3844.0
26	5589.5
27	6526.0
28	7882.5
29	9239.0
30	10449.5
31	11660.0
32	13688.5
33	15717.0
34	17914.0
35	20111.0
36	21418.0
37	22725.0
38	23927.5
39	25130.0
40	26971.5
41	28813.0
42	31279.0
43	33745.0
44	37988.0
45	42231.0
46	46228.0
47	50225.0
48	46420.0
49	42615.0
50	39698.5
51	36782.0
52	32126.5
53	27471.0
54	24058.0
55	20645.0
56	17963.5
57	15282.0
58	13658.5
59	12035.0
60	10825.0
61	9615.0
62	7895.0
63	6175.0
64	4930.5
65	3686.0
66	2802.0
67	1918.0
68	1479.0
69	1040.0
70	826.5
71	613.0
72	498.5
73	384.0
74	313.5
75	176.5
76	110.0
77	74.0
78	38.0
79	45.5
80	53.0
81	38.5
82	24.0
83	21.5
84	19.0
85	12.0
86	5.0
87	4.0
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.020799319088205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24228852324661	29.712357299786984
2	9.375774241715886	7.504519587775758
3	3.9780336406706076	4.776122580535807
4	2.5116228723632106	4.020686197603198
5	1.7177532791193468	3.437292963167054
6	1.2708682420986388	3.0516697726819215
7	0.9056883175395797	2.5372459281325908
8	0.6876591004714495	2.2016533487930077
9	0.5796959755083546	2.0879906671712636
>10	4.641114265629556	35.15323382141408
>50	0.06233143078276383	1.6704981284953089
>100	0.022908132680843817	1.806525927599268
>500	0.0015982418149425915	0.4709802636689228
>1k	0.002663736358237653	1.5692235131748629
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1999	0.4267382807079714	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1475	0.3148769204823701	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	1353	0.288832863330608	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC	1351	0.2884059115740217	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.2523284881424824	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	929	0.1983190909343199	No Hit
GTGAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	736	0.15711824642374536	No Hit
GTGAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTAT	544	0.11613087779146396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09414286232727133	0.0
2	0.0	0.0	0.0	0.5862047617929412	0.0
3	0.0	0.0	0.0	0.7527159468615844	0.0
4	0.0	0.0	0.0	1.0385601478960884	0.0
5	0.0	0.0	0.0	1.9614163697572993	0.0
6	0.0	0.0	0.0	2.4359732472029325	0.0
7	0.0	0.0	0.0	3.179509731297912	0.0
8	0.0	0.0	0.0	4.1822059316407545	0.0
9	0.0	0.0	0.0	4.527396426840749	0.0
10	0.0	0.0	0.0	5.760006148105295	0.0
11	0.0	0.0	0.0	6.687131887532368	0.0
12	0.0	0.0	0.0	8.488014396813233	0.0
13	0.0	0.0	0.0	8.84900210700692	0.0
14	0.0	0.0	0.0	9.143385343173149	0.0
15	0.0	0.0	0.0	9.401477680029545	0.0
16	0.0	0.0	0.0	9.942852507380929	0.0
17	0.0	0.0	0.0	10.68105209451858	0.0
18	0.0	0.0	0.0	11.429925475570888	0.0
19	0.0	0.0	0.0	11.893168131466984	0.0
20	0.0	0.0	0.0	12.316277322243973	0.0
21	0.0	0.0	0.0	12.833956327104818	0.0
22	0.0	0.0	0.0	13.442789531996832	0.0
23	0.0	0.0	0.0	14.067419951882536	0.0
24	0.0	0.0	0.0	14.54603287101574	0.0
25	0.0	0.0	0.0	14.957614364364899	0.0
26	0.0	0.0	0.0	15.331624103134466	0.0
27	0.0	0.0	0.0	15.724419719193829	0.0
28	0.0	0.0	0.0	16.163753076721097	0.0
29	0.0	0.0	0.0	16.60842333120569	0.0
30	0.0	0.0	0.0	17.120765439109206	0.0
31	0.0	0.0	0.0	17.59510884067655	0.0
32	0.0	0.0	0.0	18.03764433637821	0.0
33	0.0	0.0	0.0	18.482955018497684	0.0
34	0.0	0.0	0.0	18.919086237850554	0.0
35	2.1347587829313226E-4	0.0	0.0	19.388306218338858	0.0
36	2.1347587829313226E-4	0.0	0.0	19.8470658807908	0.0
37	2.1347587829313226E-4	0.0	0.0	20.282983624265377	0.0
38	2.1347587829313226E-4	0.0	0.0	20.749214942457577	0.0
39	2.1347587829313226E-4	0.0	0.0	21.21501930889319	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGGA	35	1.2098644E-7	45.000004	14
CTCGCGC	30	2.1627366E-6	45.000004	33
TCGCGCA	30	2.1627366E-6	45.000004	34
CTGTCGA	20	7.0290023E-4	45.0	43
CCGGGTT	20	7.0290023E-4	45.0	5
TACCCGT	20	7.0290023E-4	45.0	32
AAATACG	20	7.0290023E-4	45.0	1
CTATACG	20	7.0290023E-4	45.0	1
TGACGTG	20	7.0290023E-4	45.0	29
CGTTCTA	40	6.7993824E-9	45.0	35
CGCGAAG	20	7.0290023E-4	45.0	2
CGACAAG	20	7.0290023E-4	45.0	2
TTATCGA	20	7.0290023E-4	45.0	19
ATCCGGT	25	3.8872542E-5	45.0	39
CTTATCG	20	7.0290023E-4	45.0	18
CGAGGGT	25	3.8872542E-5	45.0	4
GAACGAC	25	3.8872542E-5	45.0	32
TAGTGCG	25	3.8872542E-5	45.0	1
AGGGCGC	45	3.8380676E-10	45.0	6
GATTACG	20	7.0290023E-4	45.0	1
>>END_MODULE