##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683621
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825460306222308	31.0	31.0	34.0	30.0	34.0
2	31.94969142258649	33.0	31.0	34.0	30.0	34.0
3	31.774383759422253	33.0	31.0	34.0	30.0	34.0
4	35.5609599471052	37.0	35.0	37.0	33.0	37.0
5	31.047245476660315	37.0	35.0	37.0	0.0	37.0
6	33.31389761285859	37.0	35.0	37.0	17.0	37.0
7	29.676829705348432	35.0	32.0	37.0	0.0	37.0
8	32.698913579307835	35.0	35.0	37.0	17.0	37.0
9	36.53329988400005	39.0	35.0	39.0	32.0	39.0
10	36.75552243128868	38.0	35.0	39.0	33.0	39.0
11	36.90011570738757	39.0	37.0	39.0	33.0	39.0
12	37.03138581172902	39.0	37.0	39.0	33.0	39.0
13	36.91282011523929	39.0	37.0	39.0	33.0	39.0
14	38.19125217042777	40.0	37.0	41.0	33.0	41.0
15	38.269296876485654	40.0	37.0	41.0	33.0	41.0
16	38.30776263455921	40.0	37.0	41.0	34.0	41.0
17	38.278828473671815	40.0	37.0	41.0	34.0	41.0
18	38.181031594991964	40.0	37.0	41.0	33.0	41.0
19	38.077327934630446	40.0	37.0	41.0	34.0	41.0
20	37.97527431135088	40.0	37.0	41.0	33.0	41.0
21	37.911404125970385	40.0	37.0	41.0	33.0	41.0
22	37.90030294563801	40.0	37.0	41.0	33.0	41.0
23	37.85618639567831	40.0	36.0	41.0	33.0	41.0
24	37.82752138977592	40.0	36.0	41.0	33.0	41.0
25	37.70365889871727	40.0	36.0	41.0	33.0	41.0
26	37.54561372456376	39.0	36.0	41.0	33.0	41.0
27	37.52299154063436	39.0	36.0	41.0	33.0	41.0
28	37.44085831184238	39.0	36.0	41.0	33.0	41.0
29	37.44442900379011	40.0	36.0	41.0	33.0	41.0
30	37.32383147972342	39.0	36.0	41.0	32.0	41.0
31	37.228515507861815	39.0	36.0	41.0	32.0	41.0
32	37.095879149411736	39.0	35.0	41.0	31.0	41.0
33	36.91487242199991	39.0	35.0	41.0	31.0	41.0
34	36.79815131483673	39.0	35.0	41.0	31.0	41.0
35	36.67908534114663	39.0	35.0	41.0	30.0	41.0
36	36.56557654021746	39.0	35.0	41.0	30.0	41.0
37	36.435192891967915	39.0	35.0	41.0	30.0	41.0
38	36.321420787249075	39.0	35.0	40.0	30.0	41.0
39	36.316211760610045	39.0	35.0	41.0	30.0	41.0
40	36.238244582890225	39.0	35.0	40.0	29.0	41.0
41	36.195219280858836	39.0	35.0	40.0	29.0	41.0
42	36.15861566569781	39.0	35.0	40.0	29.0	41.0
43	35.9929683260169	39.0	35.0	40.0	28.0	41.0
44	35.84200017261026	39.0	35.0	40.0	28.0	41.0
45	35.727132431566616	39.0	35.0	40.0	27.0	41.0
46	35.641769342954646	38.0	35.0	40.0	27.0	41.0
47	35.4960175301812	38.0	34.0	40.0	26.0	41.0
48	35.426634056004715	38.0	34.0	40.0	26.0	41.0
49	35.26033138244729	38.0	34.0	40.0	26.0	41.0
50	35.0990241668995	38.0	34.0	40.0	25.0	41.0
51	33.21673705167044	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	19.0
10	25.0
11	23.0
12	11.0
13	10.0
14	23.0
15	32.0
16	68.0
17	127.0
18	204.0
19	417.0
20	748.0
21	1153.0
22	1777.0
23	2805.0
24	4173.0
25	6097.0
26	7707.0
27	8823.0
28	9485.0
29	11235.0
30	13678.0
31	17350.0
32	22701.0
33	30985.0
34	43663.0
35	55182.0
36	66473.0
37	106605.0
38	137096.0
39	134902.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.274801388488655	21.8878589159783	25.476250729570914	12.361088965962134
2	31.278442294780294	25.546172513717398	25.959998303153352	17.215386888348956
3	31.953816515291365	24.664397378079375	27.823750294388265	15.558035812240995
4	26.86693357869346	26.204285708016577	28.531598648959	18.397182064330966
5	22.06222453669504	39.31520535501396	22.585175118962113	16.03739498932888
6	24.799998829760934	36.340896490891886	25.775978210148605	13.083126469198577
7	70.37817738191191	18.65960817470499	7.508985241822589	3.453229201560514
8	84.5039868582153	4.832941059446682	7.71202171963705	2.95105036270097
9	79.022148237108	7.444914653002176	9.162679320851758	4.370257789038078
10	35.86197615345345	35.75138856179081	16.897666982143615	11.48896830261212
11	24.397875430977106	26.18058836694601	31.27639437641617	18.14514182566071
12	24.42669256795798	23.8093914610581	33.13312493325981	18.63079103772412
13	21.944761790524282	25.689380519322842	33.54695072269576	18.818906967457114
14	18.530296757998947	29.075174694750455	31.970638701853805	20.423889845396793
15	18.21974456606804	29.461499866153908	34.304680517421204	18.01407505035685
16	21.53078972120517	27.75514502919015	32.043339803780164	18.670725445824512
17	21.19215179170915	27.089864120616543	30.403103474000947	21.314880613673363
18	21.940373394029734	27.5306054085524	31.583728410917743	18.945292786500122
19	21.845437749864324	29.296203598192566	29.326483534004954	19.531875117938156
20	22.46259257688105	28.370837057375358	31.198719758462655	17.967850607280937
21	22.45718022120444	28.35489255011183	31.13333265069388	18.054594577989853
22	21.749916986166312	25.55407162740758	30.93044245276257	21.765568933663538
23	19.68312851711694	27.741833559823352	31.876580736987307	20.6984571860724
24	20.0745734844307	27.471508335759143	32.085029570478376	20.36888860933178
25	20.04224563025419	29.417030781675813	30.038574005187087	20.502149582882915
26	19.269595287447284	29.837439165853596	30.12005190010254	20.77291364659658
27	20.383662877530092	29.296934997608325	30.35468483267775	19.964717292183824
28	18.69281370818041	29.320193499029436	31.707334912180873	20.279657880609285
29	19.906059059040025	27.109319345075704	30.819708581216787	22.164913014667484
30	21.15090086466039	27.76977301750531	30.706780511423727	20.37254560641057
31	22.15642878144469	28.180088089745638	28.36542470169875	21.298058427110927
32	21.632454239995553	30.11083626746399	28.421888736595278	19.834820755945177
33	21.17693868386138	29.605731830941412	28.55017619411926	20.66715329107795
34	20.03990515212376	28.36498586204929	29.31156298592349	22.283545999903456
35	19.988707193020694	29.069762339073847	28.941474881549865	22.000055586355597
36	21.603783382897834	29.921696378548933	28.1142621423274	20.360258096225834
37	20.883208678492906	29.764591784044082	29.426538974080668	19.92566056338234
38	20.54939798514089	30.875441216697556	27.63943764161721	20.93572315654434
39	20.178432201468357	29.219552939421114	28.99296540041924	21.609049458691292
40	20.51897176944535	28.679195051058993	30.048374757358243	20.753458422137413
41	19.08952475128763	28.94323024014768	29.416445662143204	22.55079934642148
42	20.302038702731483	29.16279634475828	29.208874507951045	21.32629044455919
43	21.076736963902512	28.441051401288142	28.994574479133906	21.48763715567544
44	20.286240475351107	28.755553150064145	28.50087987349716	22.45732650108759
45	20.262835694046846	28.54447127867634	28.386781564638884	22.805911462637923
46	21.35028034539606	29.41542170296114	27.473409974240116	21.760887977402685
47	19.686931794078884	28.786857045058596	29.789166804413558	21.73704435644897
48	20.55583429999956	28.424521774492007	28.966196181802488	22.05344774370594
49	20.351188743470434	27.83662292410561	29.645227399392354	22.166960933031607
50	19.351219462245894	27.72735185139134	29.647421597639628	23.27400708872314
51	19.254967299132122	27.0146762606766	28.2712204569491	25.459135983242177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	235.0
1	589.5
2	944.0
3	3700.5
4	6457.0
5	5083.5
6	3710.0
7	2926.5
8	2143.0
9	2027.5
10	1912.0
11	1926.5
12	1941.0
13	1978.0
14	2015.0
15	2038.5
16	2062.0
17	2164.5
18	2267.0
19	2481.0
20	2695.0
21	3210.5
22	3726.0
23	4102.0
24	4478.0
25	5758.5
26	8525.5
27	10012.0
28	11609.5
29	13207.0
30	16205.5
31	19204.0
32	21771.5
33	24339.0
34	26418.5
35	28498.0
36	30528.5
37	32559.0
38	34257.5
39	35956.0
40	38529.0
41	41102.0
42	44083.5
43	47065.0
44	51184.0
45	55303.0
46	59014.0
47	62725.0
48	61176.5
49	59628.0
50	56132.0
51	52636.0
52	46033.5
53	39431.0
54	34647.5
55	29864.0
56	26692.0
57	23520.0
58	21847.5
59	20175.0
60	18374.0
61	16573.0
62	14262.0
63	11951.0
64	9616.0
65	7281.0
66	5750.0
67	4219.0
68	3346.5
69	2474.0
70	2070.5
71	1667.0
72	1396.5
73	1126.0
74	839.5
75	475.0
76	397.0
77	296.5
78	196.0
79	163.5
80	131.0
81	111.0
82	91.0
83	69.5
84	48.0
85	39.5
86	31.0
87	25.5
88	20.0
89	14.0
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	683621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.21401945688154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7353547851169	28.55938301875402
2	8.86874767945546	6.778209927617679
3	3.690295371991114	4.230630574407249
4	2.231246538911847	3.410595946043076
5	1.6407953672264657	3.135069304397662
6	1.2088363258723183	2.7716696926241973
7	0.9045515343546645	2.419658495350684
8	0.748998822165062	2.289780445071763
9	0.6107765833094592	2.100620541455577
>10	5.253406510949963	39.8009350388037
>50	0.0828185574000866	1.982940111075947
>100	0.021398095988860038	1.3720824908834475
>500	0.0019813051841537075	0.5755750521849667
>1k	7.92522073661483E-4	0.572849361330105
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.4065117952783779	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	1004	0.14686500268423586	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	976	0.14276916595599023	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	944	0.13808820969513808	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	755	0.11044131177948015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4627988315162933E-4	0.0	0.0	0.075772979472544	0.0
2	1.4627988315162933E-4	0.0	0.0	0.35531383617530765	0.0
3	1.4627988315162933E-4	0.0	0.0	0.503202798041605	0.0
4	1.4627988315162933E-4	0.0	0.0	0.7357878122526956	0.0
5	2.9255976630325866E-4	0.0	0.0	1.4633839510489	0.0
6	2.9255976630325866E-4	0.0	0.0	1.9165590290526475	0.0
7	2.9255976630325866E-4	0.0	0.0	2.400599162401389	0.0
8	2.9255976630325866E-4	0.0	0.0	3.2044071203195923	0.0
9	2.9255976630325866E-4	0.0	0.0	3.513203953652682	0.0
10	2.9255976630325866E-4	0.0	0.0	4.3998063254347075	0.0
11	2.9255976630325866E-4	0.0	0.0	5.312007676768268	0.0
12	2.9255976630325866E-4	0.0	0.0	6.162625197294992	0.0
13	2.9255976630325866E-4	0.0	0.0	6.465131995652562	0.0
14	2.9255976630325866E-4	0.0	0.0	6.60058716745097	0.0
15	2.9255976630325866E-4	0.0	0.0	6.823810269140357	0.0
16	2.9255976630325866E-4	0.0	0.0	7.290443096394054	0.0
17	2.9255976630325866E-4	0.0	0.0	7.9298324656498265	0.0
18	2.9255976630325866E-4	0.0	0.0	8.583410983571307	0.0
19	2.9255976630325866E-4	0.0	0.0	8.998407012072478	0.0
20	2.9255976630325866E-4	0.0	0.0	9.385756142657993	0.0
21	2.9255976630325866E-4	0.0	0.0	9.885009383854504	0.0
22	2.9255976630325866E-4	0.0	0.0	10.454915808613253	0.0
23	2.9255976630325866E-4	0.0	0.0	11.020141277111147	0.0
24	2.9255976630325866E-4	0.0	0.0	11.46731887990568	0.0
25	2.9255976630325866E-4	0.0	0.0	11.860080366167804	0.0
26	2.9255976630325866E-4	0.0	0.0	12.239969222712585	0.0
27	2.9255976630325866E-4	0.0	0.0	12.634339787689377	0.0
28	2.9255976630325866E-4	0.0	0.0	13.026077314769442	0.0
29	2.9255976630325866E-4	0.0	0.0	13.447217098362982	0.0
30	2.9255976630325866E-4	0.0	0.0	13.92028624047535	0.0
31	2.9255976630325866E-4	0.0	0.0	14.370389440932914	0.0
32	2.9255976630325866E-4	0.0	0.0	14.790066425694938	0.0
33	2.9255976630325866E-4	0.0	0.0	15.213107847769452	0.0
34	2.9255976630325866E-4	0.0	0.0	15.6215212815288	0.0
35	2.9255976630325866E-4	0.0	0.0	16.060653490749992	0.0
36	2.9255976630325866E-4	0.0	0.0	16.48003791574571	0.0
37	2.9255976630325866E-4	0.0	0.0	16.899568620624585	0.0
38	2.9255976630325866E-4	0.0	0.0	17.32012328468552	0.0
39	2.9255976630325866E-4	0.0	0.0	17.7493084618524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	30	2.1642063E-6	45.000004	31
ACACGAC	35	1.2109558E-7	45.0	26
GTCGAGT	20	7.031278E-4	45.0	20
CCGTGAC	20	7.031278E-4	45.0	22
CGATTCG	20	7.031278E-4	45.0	10
CACGACC	25	3.8891416E-5	45.0	27
CGCGAAG	20	7.031278E-4	45.0	2
CGTAGGA	25	3.8891416E-5	45.0	4
ACTACGG	25	3.8891416E-5	45.0	2
TTGCGTT	20	7.031278E-4	45.0	26
CATACGT	20	7.031278E-4	45.0	27
CCGCGTT	20	7.031278E-4	45.0	18
TATCCGG	20	7.031278E-4	45.0	2
TACGGGA	355	0.0	43.09859	4
ACGGGTA	65	0.0	41.538464	5
GCTACGA	120	0.0	41.250004	10
CGTTTTT	1240	0.0	41.008068	1
CGTTATT	260	0.0	40.67308	1
TAGCGCG	50	1.0804797E-9	40.5	1
TCCATCG	45	1.9263098E-8	40.0	30
>>END_MODULE