##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872406
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825541089813687	31.0	31.0	34.0	30.0	34.0
2	31.9235516491175	33.0	31.0	34.0	30.0	34.0
3	31.53641194581422	33.0	31.0	34.0	30.0	34.0
4	35.45158217618861	37.0	35.0	37.0	33.0	37.0
5	31.016363940642314	37.0	35.0	37.0	0.0	37.0
6	33.30822117225237	37.0	35.0	37.0	17.0	37.0
7	29.669314516406352	35.0	32.0	37.0	0.0	37.0
8	32.67464918856587	35.0	33.0	37.0	17.0	37.0
9	36.52192098632976	39.0	35.0	39.0	32.0	39.0
10	36.872012572128114	38.0	37.0	39.0	33.0	39.0
11	36.994229750827024	39.0	37.0	39.0	33.0	39.0
12	37.075367432135955	39.0	37.0	39.0	34.0	39.0
13	36.95003817030144	39.0	37.0	39.0	33.0	39.0
14	38.278954981969406	40.0	38.0	41.0	34.0	41.0
15	38.3427899395465	40.0	38.0	41.0	34.0	41.0
16	38.357545684004926	40.0	38.0	41.0	34.0	41.0
17	38.300666203579524	40.0	37.0	41.0	34.0	41.0
18	38.16972602205853	40.0	37.0	41.0	33.0	41.0
19	38.00074735845467	40.0	37.0	41.0	34.0	41.0
20	37.86479460251305	40.0	36.0	41.0	33.0	41.0
21	37.78286142002691	40.0	36.0	41.0	33.0	41.0
22	37.747543001767525	39.0	36.0	41.0	33.0	41.0
23	37.70672255807502	39.0	36.0	41.0	33.0	41.0
24	37.64853176158807	39.0	35.0	41.0	33.0	41.0
25	37.51072321831808	39.0	35.0	41.0	33.0	41.0
26	37.344918535635934	39.0	35.0	41.0	33.0	41.0
27	37.33154632132287	39.0	35.0	41.0	33.0	41.0
28	37.20437044220237	39.0	35.0	41.0	32.0	41.0
29	37.18464224225877	39.0	35.0	41.0	32.0	41.0
30	37.051274292015414	39.0	35.0	41.0	32.0	41.0
31	36.92535470870214	39.0	35.0	41.0	31.0	41.0
32	36.784729816163576	39.0	35.0	41.0	31.0	41.0
33	36.50712397668058	39.0	35.0	41.0	30.0	41.0
34	36.232765478458425	39.0	35.0	41.0	30.0	41.0
35	36.00967897974108	39.0	35.0	41.0	28.0	41.0
36	35.81597100432597	39.0	35.0	40.0	27.0	41.0
37	35.68335041253728	39.0	35.0	40.0	26.0	41.0
38	35.56372147830253	39.0	35.0	40.0	25.0	41.0
39	35.50774180828651	39.0	35.0	40.0	25.0	41.0
40	35.35485656907449	39.0	35.0	40.0	24.0	41.0
41	35.230125652505826	38.0	35.0	40.0	24.0	41.0
42	35.15575890124552	38.0	34.0	40.0	24.0	41.0
43	34.96202800072443	38.0	34.0	40.0	23.0	41.0
44	34.73488375825017	38.0	34.0	40.0	22.0	41.0
45	34.66613251169753	38.0	34.0	40.0	22.0	41.0
46	34.50932364059853	38.0	34.0	40.0	22.0	41.0
47	34.34880090233217	38.0	33.0	40.0	20.0	41.0
48	34.27198689600943	38.0	33.0	40.0	20.0	41.0
49	34.10348965963095	38.0	33.0	40.0	20.0	41.0
50	33.95727218749069	38.0	33.0	40.0	20.0	41.0
51	32.06020132828064	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	24.0
9	40.0
10	47.0
11	34.0
12	24.0
13	23.0
14	34.0
15	43.0
16	79.0
17	198.0
18	390.0
19	749.0
20	1299.0
21	2097.0
22	3308.0
23	5139.0
24	7683.0
25	11321.0
26	14502.0
27	15506.0
28	16227.0
29	17231.0
30	19535.0
31	23566.0
32	29513.0
33	40153.0
34	56777.0
35	69660.0
36	81911.0
37	129710.0
38	164243.0
39	161321.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.074224615603285	19.693812284647287	24.02264541967845	15.209317680070978
2	34.900952079650985	24.51266955981504	25.324103685669286	15.262274674864685
3	31.170922712590237	24.314596644222988	29.21953769231298	15.294942950873791
4	26.565956676134732	24.627065838611838	31.19808896316623	17.60888852208719
5	21.479678039811738	38.46041865828525	24.490432207022877	15.569471094880136
6	24.089930605704225	34.689467977065725	27.99098126331089	13.22962015391916
7	66.99908070325054	18.71147149377698	10.890915468256752	3.398532334715717
8	80.59447092294184	4.988846935944961	11.411315373805316	3.0053667673078817
9	74.90801301229016	7.263590575947437	12.758279975149184	5.070116436613229
10	37.32470890846693	30.11739946767904	20.22934276013691	12.328548863717122
11	26.625332700600406	25.47884815097558	30.98350997127484	16.912309177149172
12	25.463144453385233	22.90080535897277	32.982006084323125	18.654044103318867
13	22.265436046978127	23.760382207366753	34.82243359169927	19.151748153955843
14	19.416418502394528	27.135530933991742	33.228450973514626	20.219599590099104
15	19.30190759806787	26.80987980366939	36.133405776668205	17.75480682159453
16	22.386824483096174	25.932879874737218	33.76455457665353	17.915741065513075
17	21.92557135095357	25.16099155668347	32.50424687588118	20.409190216481775
18	22.376622810938944	26.2608235156567	32.575085453332505	18.787468220071847
19	22.59888171333072	27.82695213008622	30.67768905761767	18.896477098965388
20	24.33683399701515	27.13851119776801	31.514684676629916	17.009970128586918
21	23.40596006905042	26.964509643445826	31.499439481159	18.130090806344754
22	22.53320128472294	24.50819916415064	32.11566632966761	20.842933221458818
23	21.66949791725412	26.88851291715096	32.520294450061094	18.921694715533825
24	22.16536795941339	25.047168405535952	32.87219482672059	19.915268808330065
25	21.294328558033758	27.386560844377506	30.93261623601855	20.386494361570186
26	20.631678369933265	28.277659713482024	31.143183334364966	19.947478582219745
27	21.356226344156276	27.68137770716845	31.41106319763963	19.55133275103564
28	19.6289342347485	27.772161126814808	32.92939296611899	19.669511672317704
29	21.227157997537844	26.911438023122265	31.950949443263806	19.910454536076095
30	21.762344596437895	26.53340302565549	31.910601256754312	19.79365112115231
31	22.039509127630943	26.318365531644673	30.46907059327882	21.17305474744557
32	22.88510166138243	26.98307897928258	31.159230908544878	18.972588450790116
33	22.049252297668744	27.565032794364093	30.191791436555913	20.193923471411246
34	22.025295561928733	27.52342372702618	30.850658982171147	19.60062172887394
35	21.8962272153103	27.402035290908135	30.539565294140576	20.16217219964099
36	21.505124907439885	29.813641813559283	29.17976263345277	19.501470645548057
37	22.1822179123023	28.46679183774527	30.267673537320928	19.083316712631504
38	21.396574530665767	28.81479494638964	28.928388846477443	20.86024167646715
39	22.72645992806102	27.504854391189422	29.5745329582786	20.19415272247096
40	23.273911458655718	26.856761645380704	30.042434371152883	19.826892524810695
41	20.961914521449877	28.28671513034069	29.918065671258564	20.83330467695087
42	21.88877655586963	27.590250410932526	29.47457949624372	21.046393536954124
43	21.762115345378184	27.440205592350353	29.433658182084947	21.36402088018652
44	21.220051214686737	27.84540684039312	29.119240353688536	21.815301591231606
45	21.49698649482007	26.418318993679545	29.33817511571447	22.746519395785906
46	21.38797761592653	28.724584654392565	28.440313340348418	21.44712438933249
47	20.901506867215495	27.01654963400068	30.76377283053991	21.318170668243912
48	21.245498082314885	26.485374928645605	30.75861468169637	21.51051230734314
49	20.831814545062734	26.48560417970532	30.692361125439305	21.99022014979264
50	20.452977168886964	26.56251791023904	30.442133593762538	22.54237132711146
51	20.055685082404292	26.271942192052784	30.332207710630144	23.34016501491278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	369.0
1	907.0
2	1445.0
3	9468.5
4	17492.0
5	13551.0
6	9610.0
7	7619.0
8	5628.0
9	5368.5
10	5109.0
11	4856.0
12	4603.0
13	4607.5
14	4612.0
15	4424.0
16	4236.0
17	4289.5
18	4343.0
19	4298.5
20	4254.0
21	4336.5
22	4419.0
23	5017.0
24	5615.0
25	6870.5
26	9845.0
27	11564.0
28	13977.5
29	16391.0
30	18157.0
31	19923.0
32	22824.0
33	25725.0
34	27752.0
35	29779.0
36	31823.0
37	33867.0
38	36916.0
39	39965.0
40	43425.0
41	46885.0
42	49315.5
43	51746.0
44	55817.5
45	59889.0
46	65829.5
47	71770.0
48	69437.5
49	67105.0
50	64405.5
51	61706.0
52	56256.5
53	50807.0
54	46685.5
55	42564.0
56	39330.5
57	36097.0
58	34762.5
59	33428.0
60	31583.0
61	29738.0
62	26495.5
63	23253.0
64	18987.5
65	14722.0
66	11818.5
67	8915.0
68	7446.0
69	5977.0
70	5004.0
71	4031.0
72	3304.0
73	2577.0
74	2122.0
75	1356.0
76	1045.0
77	839.0
78	633.0
79	473.5
80	314.0
81	251.0
82	188.0
83	149.0
84	110.0
85	92.5
86	75.0
87	55.0
88	35.0
89	36.5
90	38.0
91	23.0
92	8.0
93	6.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	872406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.53405061382354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22609050612498	28.602319284292665
2	8.735482231881809	6.732270289189797
3	3.9886085770187756	4.610917343567165
4	2.4561173519082007	3.7857660140768337
5	1.746820677365455	3.3656038197436975
6	1.2838950196254633	2.9684205401450137
7	0.9656953328092135	2.6048506982402375
8	0.7544746794198617	2.3258372386890587
9	0.623892236850119	2.1636985529118657
>10	5.122764596175087	37.554206444607324
>50	0.07559659818461524	1.8265935486954685
>100	0.017235955592277863	1.1546091708393031
>500	0.0012095407432857253	0.25121983775255846
>1k	0.001814311114928588	1.1724369236856416
>5k	3.0238518582143133E-4	0.8812502935633577
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7563	0.8669128823047983	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2493	0.2857614459322838	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	1843	0.2112548515255512	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	1642	0.1882151200243923	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	1561	0.17893045210601485	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1501	0.17205292031462416	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.11714729151335501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.438765895695353E-4	0.0	0.0	0.05249849267428239	0.0
2	3.438765895695353E-4	0.0	0.0	0.26180471019227286	0.0
3	3.438765895695353E-4	0.0	0.0	0.3911023078704181	0.0
4	3.438765895695353E-4	0.0	0.0	0.6116418273143468	0.0
5	3.438765895695353E-4	0.0	0.0	1.3413479503809007	0.0
6	3.438765895695353E-4	0.0	0.0	1.7762372106565063	0.0
7	3.438765895695353E-4	0.0	0.0	2.163442250511803	0.0
8	3.438765895695353E-4	0.0	0.0	2.7786374692517017	0.0
9	3.438765895695353E-4	0.0	0.0	3.0601577705792944	0.0
10	3.438765895695353E-4	0.0	0.0	3.891078236509148	0.0
11	3.438765895695353E-4	0.0	0.0	4.489194251300427	0.0
12	3.438765895695353E-4	0.0	0.0	5.237813586793305	0.0
13	3.438765895695353E-4	0.0	0.0	5.493887020492752	0.0
14	3.438765895695353E-4	0.0	0.0	5.629603647842862	0.0
15	3.438765895695353E-4	0.0	0.0	5.8303129506216145	0.0
16	3.438765895695353E-4	0.0	0.0	6.157339587302243	0.0
17	3.438765895695353E-4	0.0	0.0	6.549358899411512	0.0
18	3.438765895695353E-4	0.0	0.0	6.981841023560132	0.0
19	3.438765895695353E-4	0.0	0.0	7.2773456395302185	0.0
20	3.438765895695353E-4	0.0	0.0	7.549695898469291	0.0
21	3.438765895695353E-4	0.0	0.0	7.890936100852127	0.0
22	3.438765895695353E-4	0.0	0.0	8.273899996102733	0.0
23	3.438765895695353E-4	0.0	0.0	8.666950937980712	0.0
24	3.438765895695353E-4	0.0	0.0	8.969447711272045	0.0
25	3.438765895695353E-4	0.0	0.0	9.2423710978604	0.0
26	3.438765895695353E-4	0.0	0.0	9.495693518843291	0.0
27	3.438765895695353E-4	0.0	0.0	9.75268395678159	0.0
28	3.438765895695353E-4	0.0	0.0	10.03661139423617	0.0
29	3.438765895695353E-4	0.0	0.0	10.33005275066884	0.0
30	3.438765895695353E-4	0.0	0.0	10.66487392337971	0.0
31	3.438765895695353E-4	0.0	0.0	10.995224700426178	0.0
32	3.438765895695353E-4	0.0	0.0	11.321907460517236	0.0
33	3.438765895695353E-4	0.0	0.0	11.644578327063316	0.0
34	3.438765895695353E-4	0.0	0.0	11.94810673012336	0.0
35	3.438765895695353E-4	0.0	0.0	12.27834288163997	0.0
36	3.438765895695353E-4	0.0	0.0	12.605484143850456	0.0
37	3.438765895695353E-4	0.0	0.0	12.938700559143335	0.0
38	3.438765895695353E-4	0.0	0.0	13.294842080407516	0.0
39	3.438765895695353E-4	0.0	0.0	13.640552678454757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGG	35	1.2114833E-7	45.000004	2
CGTATAG	20	7.032352E-4	45.0	2
CGCATGG	55	1.8189894E-12	45.0	2
TCGCTAA	20	7.032352E-4	45.0	11
TAAGACG	20	7.032352E-4	45.0	1
TATAGCG	30	2.1648975E-6	44.999996	1
CGTTATT	655	0.0	42.59542	1
CGTTTTA	450	0.0	42.0	1
CGTTTTT	3420	0.0	41.973686	1
TACGGGT	130	0.0	41.538464	4
CGGTTTT	865	0.0	40.83815	1
ACACGTG	80	0.0	39.375	42
CACGTGA	80	0.0	39.375	43
GCTACGA	115	0.0	39.130432	10
TCTACGG	65	9.094947E-12	38.076927	2
TCGTCGA	30	1.1396964E-4	37.499996	41
ACCCCGC	30	1.1396964E-4	37.499996	17
CTACGGG	175	0.0	37.285717	3
CTCGTAA	145	0.0	37.24138	33
AGGCGAT	200	0.0	37.125	7
>>END_MODULE