FastQCFastQC Report
Sat 14 Jan 2017
SRR2936060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958741
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124661.3002468862810705No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.38613139523604395No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.2543961299245573No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16590.1730394340077247No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT10440.10889280838099133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT207.0326997E-445.00000436
CGGTCTA351.211647E-745.031
CGTTTTA6250.043.561
CGTTATT10750.042.906981
CGTTTTT51150.042.1407621
CTACGGG2400.040.3125043
GCGATAA900.040.09
CGGTTTT14550.039.432991
CGGTTTA1150.039.1304361
CGGGCTA700.038.5714266
TATTACG301.1397805E-437.5000041
TATCGCG301.1397805E-437.5000041
ACGGGAT3850.037.4025965
GACCGAT3900.036.9230779
TGTACGG1300.036.3461532
CTTCGCA250.002106934336.024
TTCGTCG250.002106934336.037
TACGGGT1000.036.04
ATAACGG751.8189894E-1236.02
CGGTATG250.002106934336.01
Produced by FastQC (version 0.11.5)