##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936060.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958741
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717082089949216	31.0	31.0	34.0	30.0	34.0
2	31.819913824484402	33.0	31.0	34.0	30.0	34.0
3	31.290456963872412	31.0	31.0	34.0	28.0	34.0
4	35.30608579376495	37.0	35.0	37.0	33.0	37.0
5	30.951881686503445	37.0	35.0	37.0	0.0	37.0
6	33.25511895287674	37.0	35.0	37.0	17.0	37.0
7	29.62541291130764	35.0	32.0	37.0	0.0	37.0
8	32.65136778337424	35.0	33.0	37.0	17.0	37.0
9	36.47393613082156	39.0	35.0	39.0	32.0	39.0
10	36.82972877972257	38.0	37.0	39.0	33.0	39.0
11	36.97395751303011	39.0	37.0	39.0	33.0	39.0
12	37.02254310601091	39.0	37.0	39.0	33.0	39.0
13	36.91689517815552	39.0	37.0	39.0	33.0	39.0
14	38.145657690658894	40.0	37.0	41.0	33.0	41.0
15	38.21928132832538	40.0	37.0	41.0	33.0	41.0
16	38.236429859576255	40.0	37.0	41.0	33.0	41.0
17	38.19226151796992	40.0	37.0	41.0	33.0	41.0
18	38.025694113425835	40.0	37.0	41.0	33.0	41.0
19	37.8625708090089	40.0	37.0	41.0	33.0	41.0
20	37.662170492343606	39.0	35.0	41.0	33.0	41.0
21	37.54271382990818	39.0	35.0	41.0	33.0	41.0
22	37.49866648031116	39.0	35.0	41.0	33.0	41.0
23	37.41090972431554	39.0	35.0	41.0	33.0	41.0
24	37.341512462698475	39.0	35.0	41.0	33.0	41.0
25	37.19577550141279	39.0	35.0	41.0	32.0	41.0
26	37.04118317668693	39.0	35.0	41.0	32.0	41.0
27	36.9853182454907	39.0	35.0	41.0	32.0	41.0
28	36.85047578021593	39.0	35.0	40.0	32.0	41.0
29	36.78047877372512	39.0	35.0	40.0	31.0	41.0
30	36.57437201496546	39.0	35.0	40.0	31.0	41.0
31	36.38537832428153	39.0	35.0	40.0	30.0	41.0
32	36.16116552854212	38.0	35.0	40.0	30.0	41.0
33	35.824574102912045	38.0	35.0	40.0	28.0	41.0
34	35.44420755970591	38.0	34.0	40.0	25.0	41.0
35	35.09235445234949	38.0	34.0	40.0	24.0	41.0
36	34.88403437424706	38.0	34.0	40.0	23.0	41.0
37	34.689426028510304	38.0	34.0	40.0	22.0	41.0
38	34.47706106237242	38.0	34.0	40.0	21.0	41.0
39	34.363980470220845	38.0	33.0	40.0	20.0	41.0
40	34.20888331676647	38.0	33.0	40.0	18.0	41.0
41	34.05354000715522	38.0	33.0	40.0	18.0	41.0
42	33.97424747663863	38.0	33.0	40.0	18.0	41.0
43	33.81325822093767	38.0	33.0	40.0	18.0	41.0
44	33.596746149377154	38.0	33.0	40.0	15.0	41.0
45	33.439151971178866	37.0	33.0	40.0	15.0	41.0
46	33.36841128104462	37.0	32.0	40.0	15.0	41.0
47	33.199380228862644	37.0	32.0	40.0	15.0	41.0
48	33.098399880676844	37.0	32.0	40.0	14.0	41.0
49	32.937846613423226	37.0	31.0	40.0	13.0	41.0
50	32.76668777073266	36.0	31.0	40.0	13.0	41.0
51	30.93058500679537	35.0	27.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	54.0
10	59.0
11	48.0
12	26.0
13	46.0
14	44.0
15	76.0
16	163.0
17	289.0
18	580.0
19	993.0
20	1826.0
21	3048.0
22	4820.0
23	7455.0
24	11708.0
25	16812.0
26	21448.0
27	22772.0
28	22611.0
29	22843.0
30	24727.0
31	28824.0
32	35558.0
33	47231.0
34	65292.0
35	76448.0
36	90361.0
37	137873.0
38	164346.0
39	150322.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.36714920922334	19.710015530784645	22.492414531140316	18.430420728851693
2	38.27321455951086	22.019919874084867	23.70713258325241	15.999732983151862
3	31.66381744391864	21.968706877039786	30.50771793424919	15.859757744792388
4	26.272684697952837	24.194438331102976	31.939908692754354	17.592968278189836
5	20.504807867818318	37.719676116907486	25.89187277898828	15.883643236285922
6	23.34812008665531	32.850477866285054	30.432723749166872	13.368678297892759
7	64.84952661876356	18.572482036337238	12.691123045744368	3.886868299154829
8	78.22529755168497	4.37344392281127	13.91303803634141	3.488220489162349
9	72.39932369638933	6.545563400334396	16.0036965144914	5.051416388784875
10	36.90496181971982	26.76364106677403	22.81158310742943	13.51981400607672
11	27.351599649957603	24.658797318566744	30.942350436666423	17.047252594809233
12	24.536762274691494	22.516717236459062	34.36684151402725	18.57967897482219
13	22.765480979743224	21.661637501681895	36.11590617278285	19.45697534579203
14	19.742245298782464	24.302392408377234	35.888003120759414	20.067359172080884
15	19.78031606033329	24.460099234308327	37.27158846862709	18.4879962367313
16	23.899989673957826	23.826560040720068	34.672450640996885	17.600999644325213
17	23.102381143603957	23.908333950462115	33.57955902584744	19.40972588008649
18	23.14817036092125	24.455092668405754	34.10055479008408	18.296182180588918
19	22.817737011351344	26.1423053775733	32.31842593568023	18.721531675395127
20	23.67344256686634	25.760659030958312	31.980482737256466	18.585415664918887
21	23.98687445305875	25.278151242097707	33.14273615084783	17.59223815399571
22	22.90138838330686	22.9457173522359	33.64704336207589	20.505850902381354
23	21.66268053624493	24.638562448043842	33.85262547445035	19.846131541260885
24	21.732876762337273	24.462915427628527	34.044961047874246	19.759246762159957
25	22.409910497204148	25.109179642885827	32.22298827316241	20.25792158674762
26	21.007967741027034	25.301515216309724	32.44327717287568	21.247239869787567
27	21.095582644322086	24.460307841220935	33.429779262595424	21.01433025186156
28	20.29724398977409	25.29129347759197	33.19739116195093	21.214071370683012
29	21.78023053149912	24.95481052755645	32.50721519158981	20.757743749354624
30	22.753903296093522	23.34061023780145	33.54096674701509	20.364519719089934
31	22.966786650409237	23.86358776770786	31.95805749415118	21.21156808773172
32	23.080164507411283	24.039443395035782	32.10835877468472	20.772033322868218
33	22.875521126143557	23.84314429027235	31.92290722937686	21.358427354207237
34	21.470657873189943	24.31480451967737	33.44678072597292	20.76775688115977
35	21.46878041097648	24.770819230636846	33.14273615084783	20.617664207538844
36	22.11076818452533	25.97990489610854	31.54845782124682	20.360869098119306
37	21.348727132770996	26.348721917598183	32.112843823305774	20.189707126325047
38	21.624818381606712	26.042069756065505	32.205152382134486	20.127959480193294
39	21.82445519697186	25.262297116739557	31.337139018775666	21.576108667512916
40	22.92725564047016	24.24794600418674	32.564269182187886	20.260529173155213
41	20.61265764163627	25.41843939082609	32.13213996272194	21.83676300481569
42	20.959153723476934	26.064703606083395	31.20477793272636	21.771364737713313
43	20.737195968462807	25.361594007140614	31.66392174737494	22.237288277021637
44	21.664557998458395	24.934888567402457	31.28592602173058	22.114627412408566
45	21.595717717297997	24.989647881961865	30.880394183622062	22.534240217118075
46	22.141850614503813	25.41916951502022	30.365552323307337	22.07342754716863
47	20.86142138492043	25.556537166972106	31.81151113804458	21.770530310062885
48	20.872373247832314	25.19679454618088	31.570674457439495	22.360157748547312
49	21.467215859131926	24.955436348294274	31.69677733611059	21.880570456463218
50	20.477793272635676	24.792931563373216	32.329899315873625	22.39937584811748
51	20.567702851969404	24.036940112084494	31.957953190694884	23.43740384525122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	832.0
2	1344.0
3	13781.5
4	26219.0
5	20087.0
6	13955.0
7	11034.0
8	8113.0
9	7707.5
10	7302.0
11	7087.5
12	6873.0
13	6577.0
14	6281.0
15	5922.0
16	5563.0
17	5235.5
18	4908.0
19	4972.0
20	5036.0
21	5232.0
22	5428.0
23	5741.5
24	6055.0
25	6664.0
26	8418.5
27	9564.0
28	11300.0
29	13036.0
30	15614.0
31	18192.0
32	21096.5
33	24001.0
34	26266.0
35	28531.0
36	30852.0
37	33173.0
38	35428.5
39	37684.0
40	40116.5
41	42549.0
42	45754.0
43	48959.0
44	53396.5
45	57834.0
46	64004.0
47	70174.0
48	72952.5
49	75731.0
50	73462.5
51	71194.0
52	65412.5
53	59631.0
54	55452.0
55	51273.0
56	47740.5
57	44208.0
58	42234.5
59	40261.0
60	37892.5
61	35524.0
62	32242.5
63	28961.0
64	25435.0
65	21909.0
66	18190.5
67	14472.0
68	11867.5
69	9263.0
70	8063.0
71	6863.0
72	5676.5
73	4490.0
74	3698.5
75	2302.5
76	1698.0
77	1298.5
78	899.0
79	675.0
80	451.0
81	367.0
82	283.0
83	211.5
84	140.0
85	119.0
86	98.0
87	78.5
88	59.0
89	41.5
90	24.0
91	20.0
92	16.0
93	14.5
94	13.0
95	8.0
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	958741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.237279762259504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42013914015786	31.010669570468668
2	9.256263789787605	7.819188064850631
3	4.447994607621521	5.636135778693956
4	2.7077563885169007	4.574730564393351
5	1.8334690241914031	3.8720372055104617
6	1.3977970310624612	3.542348655110455
7	1.0572407350356474	3.1258480891230254
8	0.8218286215820902	2.7769444325115877
9	0.6734108001101304	2.559873632326074
>10	4.341184741083275	30.885801594361244
>50	0.0286937125420142	0.824008983814001
>100	0.012225421183937882	0.9477997132116041
>500	7.484951719351065E-4	0.17893750387391572
>1k	9.97993562580142E-4	0.9319925113432924
>5k	0.0	0.0
>10k+	2.494983906450355E-4	1.3136837004076756
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12466	1.3002468862810705	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3702	0.38613139523604395	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2439	0.2543961299245573	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1659	0.1730394340077247	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT	1044	0.10889280838099133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0430345630363154E-4	0.0	0.0	0.07301241941254208	0.0
2	1.0430345630363154E-4	0.0	0.0	0.22383521722759328	0.0
3	1.0430345630363154E-4	0.0	0.0	0.41272877659346996	0.0
4	1.0430345630363154E-4	0.0	0.0	0.5665763746413265	0.0
5	1.0430345630363154E-4	0.0	0.0	1.040635583541332	0.0
6	1.0430345630363154E-4	0.0	0.0	1.793289324228337	0.0
7	1.0430345630363154E-4	0.0	0.0	2.3722778101697957	0.0
8	1.0430345630363154E-4	0.0	0.0	3.2548936574111256	0.0
9	1.0430345630363154E-4	0.0	0.0	3.80791058273298	0.0
10	1.0430345630363154E-4	0.0	0.0	4.513106250801833	0.0
11	1.0430345630363154E-4	0.0	0.0	5.139761416274051	0.0
12	1.0430345630363154E-4	0.0	0.0	5.6186185841640235	0.0
13	1.0430345630363154E-4	0.0	0.0	5.902532592222508	0.0
14	1.0430345630363154E-4	0.0	0.0	6.087879834074061	0.0
15	1.0430345630363154E-4	0.0	0.0	6.2501760120825125	0.0
16	1.0430345630363154E-4	0.0	0.0	6.5142723634433075	0.0
17	1.0430345630363154E-4	0.0	0.0	6.80559191689935	0.0
18	1.0430345630363154E-4	0.0	0.0	7.147602950118958	0.0
19	1.0430345630363154E-4	0.0	0.0	7.366431601443977	0.0
20	1.0430345630363154E-4	0.0	0.0	7.589849604846356	0.0
21	1.0430345630363154E-4	0.0	0.0	7.847479141916326	0.0
22	1.0430345630363154E-4	0.0	0.0	8.135878198595867	0.0
23	1.0430345630363154E-4	0.0	0.0	8.441591629021811	0.0
24	1.0430345630363154E-4	0.0	0.0	8.68858221354881	0.0
25	1.0430345630363154E-4	0.0	0.0	8.924516631707625	0.0
26	1.0430345630363154E-4	0.0	0.0	9.148247545478915	0.0
27	2.0860691260726308E-4	0.0	0.0	9.386163729307498	0.0
28	2.0860691260726308E-4	0.0	0.0	9.633571527659711	0.0
29	2.0860691260726308E-4	0.0	0.0	9.897354968651596	0.0
30	2.0860691260726308E-4	0.0	0.0	10.209117999543151	0.0
31	2.0860691260726308E-4	0.0	0.0	10.480306985932593	0.0
32	2.0860691260726308E-4	0.0	0.0	10.736893488439527	0.0
33	2.0860691260726308E-4	0.0	0.0	11.004223246945735	0.0
34	2.0860691260726308E-4	0.0	0.0	11.273326164209104	0.0
35	2.0860691260726308E-4	0.0	0.0	11.568087731723166	0.0
36	2.0860691260726308E-4	0.0	0.0	11.850437187937096	0.0
37	3.129103689108946E-4	0.0	0.0	12.147180521120928	0.0
38	3.129103689108946E-4	0.0	0.0	12.474589070458027	0.0
39	3.129103689108946E-4	0.0	0.0	12.914436745690441	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATGT	20	7.0326997E-4	45.000004	36
CGGTCTA	35	1.211647E-7	45.0	31
CGTTTTA	625	0.0	43.56	1
CGTTATT	1075	0.0	42.90698	1
CGTTTTT	5115	0.0	42.140762	1
CTACGGG	240	0.0	40.312504	3
GCGATAA	90	0.0	40.0	9
CGGTTTT	1455	0.0	39.43299	1
CGGTTTA	115	0.0	39.130436	1
CGGGCTA	70	0.0	38.571426	6
TATTACG	30	1.1397805E-4	37.500004	1
TATCGCG	30	1.1397805E-4	37.500004	1
ACGGGAT	385	0.0	37.402596	5
GACCGAT	390	0.0	36.923077	9
TGTACGG	130	0.0	36.346153	2
CTTCGCA	25	0.0021069343	36.0	24
TTCGTCG	25	0.0021069343	36.0	37
TACGGGT	100	0.0	36.0	4
ATAACGG	75	1.8189894E-12	36.0	2
CGGTATG	25	0.0021069343	36.0	1
>>END_MODULE