##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681895
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893949948305824	31.0	31.0	34.0	30.0	34.0
2	32.02132293094978	33.0	31.0	34.0	30.0	34.0
3	31.754108770411868	33.0	31.0	34.0	30.0	34.0
4	35.58746141268084	37.0	35.0	37.0	33.0	37.0
5	31.04019680449336	37.0	35.0	37.0	0.0	37.0
6	33.3494306308156	37.0	35.0	37.0	17.0	37.0
7	29.73021946194062	35.0	32.0	37.0	0.0	37.0
8	32.764604521223944	35.0	35.0	37.0	17.0	37.0
9	36.626301703341426	39.0	35.0	39.0	32.0	39.0
10	36.90420812588448	38.0	37.0	39.0	33.0	39.0
11	37.00574281964232	39.0	37.0	39.0	34.0	39.0
12	37.07803107516553	39.0	37.0	39.0	34.0	39.0
13	36.93381092396923	39.0	37.0	39.0	33.0	39.0
14	38.15895849067672	40.0	38.0	41.0	33.0	41.0
15	38.25053857265415	40.0	38.0	41.0	33.0	41.0
16	38.31335176236811	40.0	38.0	41.0	34.0	41.0
17	38.29184991824255	40.0	37.0	41.0	34.0	41.0
18	38.17805087293498	40.0	37.0	41.0	34.0	41.0
19	38.047695026360365	40.0	37.0	41.0	34.0	41.0
20	37.92756069482838	40.0	36.0	41.0	33.0	41.0
21	37.850620696734836	40.0	36.0	41.0	33.0	41.0
22	37.841974204239655	40.0	36.0	41.0	33.0	41.0
23	37.79598618555643	40.0	36.0	41.0	33.0	41.0
24	37.7345603062055	40.0	36.0	41.0	33.0	41.0
25	37.61682370452929	39.0	36.0	41.0	33.0	41.0
26	37.47330014151739	39.0	35.0	41.0	33.0	41.0
27	37.448290425945345	39.0	35.0	41.0	33.0	41.0
28	37.34929571268304	39.0	35.0	41.0	33.0	41.0
29	37.32044816284032	39.0	35.0	41.0	33.0	41.0
30	37.201762734731886	39.0	35.0	41.0	32.0	41.0
31	37.04388798861995	39.0	35.0	41.0	31.0	41.0
32	36.92639189318003	39.0	35.0	41.0	31.0	41.0
33	36.72226955762984	39.0	35.0	41.0	31.0	41.0
34	36.50951099509455	39.0	35.0	41.0	30.0	41.0
35	36.34331385330586	39.0	35.0	41.0	30.0	41.0
36	36.17331407328108	39.0	35.0	41.0	29.0	41.0
37	36.022611985716274	39.0	35.0	40.0	29.0	41.0
38	35.85085826996825	39.0	35.0	40.0	27.0	41.0
39	35.78927694146459	39.0	35.0	40.0	27.0	41.0
40	35.619768439422494	39.0	35.0	40.0	26.0	41.0
41	35.49513488147002	39.0	35.0	40.0	25.0	41.0
42	35.41807169725544	39.0	35.0	40.0	25.0	41.0
43	35.28691220789124	38.0	35.0	40.0	24.0	41.0
44	35.103873763555974	38.0	34.0	40.0	24.0	41.0
45	34.98580719905557	38.0	34.0	40.0	23.0	41.0
46	34.85091253052156	38.0	34.0	40.0	23.0	41.0
47	34.65812331810616	38.0	34.0	40.0	23.0	41.0
48	34.554171829973825	38.0	34.0	40.0	22.0	41.0
49	34.418793215964335	38.0	34.0	40.0	22.0	41.0
50	34.25987285432508	38.0	33.0	40.0	21.0	41.0
51	32.35589790216969	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	33.0
10	32.0
11	38.0
12	13.0
13	21.0
14	19.0
15	35.0
16	59.0
17	137.0
18	281.0
19	514.0
20	929.0
21	1536.0
22	2236.0
23	3536.0
24	5293.0
25	7549.0
26	9854.0
27	10757.0
28	11300.0
29	12471.0
30	14590.0
31	18063.0
32	23006.0
33	31264.0
34	44301.0
35	54921.0
36	65863.0
37	103673.0
38	131444.0
39	128103.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90614390778639	20.69893458670323	26.096979740282595	13.297941765227783
2	33.93000388622882	23.993283423400964	25.861899559316313	16.2148131310539
3	31.706934352062998	24.16442414154672	28.715271412754163	15.413370093636116
4	26.385880524127614	25.6976513979425	29.905044031705764	18.011424046224125
5	21.578688801061745	39.238152501484834	23.472675411903595	15.710483285549829
6	24.742812309813093	34.701530294253516	26.92115355003336	13.634503845900028
7	69.75780728704567	18.47307869979982	8.51700041795291	3.252113595201607
8	84.9884513011534	4.142866570366405	8.312276816811973	2.5564053116682186
9	78.95453112282684	6.765411097016402	9.95211872795665	4.327939052200119
10	38.181978163793545	33.39311770873815	16.575132535067716	11.84977159240059
11	26.597203381752323	25.80309285153873	29.96531724092419	17.634386525784763
12	24.568445288497497	23.35036919173773	32.965925839022134	19.115259680742636
13	23.038590985415645	24.09843157670902	34.374647123090796	18.488330314784534
14	20.128612176361464	27.870713232975753	32.44326472550759	19.557409865155194
15	19.242552005807344	26.774503405949595	36.108198476305006	17.874746111938055
16	23.66757345339092	25.787841236554016	32.12122100909964	18.423364300955424
17	23.410202450523908	25.50685956048952	30.476979593632453	20.605958395354122
18	23.553919591726	25.27764538528659	31.65384699990468	19.514588023082734
19	23.42868036867846	27.55174916959356	29.992007567147432	19.027562894580544
20	24.60100162048409	27.24187741514456	30.193944815550783	17.963176148820565
21	23.830648413612067	26.817618548310225	31.366266067356413	17.985466970721298
22	22.42925963674759	24.18847476517646	31.799910543412107	21.58235505466384
23	21.02244480455202	26.884491014012426	31.844052236781323	20.249011944654235
24	21.102515783221758	26.612894947169284	32.300280835025916	19.98430843458304
25	21.11820734863872	27.588558355758586	30.845218105426785	20.448016190175906
26	20.613730852990564	28.107993166103284	30.389869408046692	20.88840657285946
27	20.81170854750365	27.633286649704132	31.408941259284788	20.14606354350743
28	19.302238614449436	27.188203462409906	32.48198036354571	21.027577559594953
29	20.16160845878031	26.317394906840498	32.198065684599534	21.322930949779657
30	21.71844638837358	25.621833273451188	32.523482354321416	20.136237983853817
31	22.59453434913	27.13863571370959	30.22342149451162	20.043408442648794
32	22.85982445977753	26.841669171939962	29.904310780985345	20.394195587297165
33	22.492759149135864	26.685193468202584	29.611303793105975	21.210743589555577
34	21.8736022408142	26.48838897484217	30.903291562483957	20.73471722185967
35	20.78765792387391	27.130130005352733	30.984389092162285	21.097822978611077
36	22.565644270745498	27.585918653165077	29.619076250742417	20.229360825347012
37	21.002940335388878	28.510987762045474	29.47975861386284	21.006313288702806
38	21.80511662352708	28.32268897704192	28.67904882716547	21.193145572265525
39	22.67255222578256	26.515225951209498	28.565834915932804	22.246386907075134
40	23.16734981192119	26.75045278231986	28.959737202941803	21.12246020281715
41	20.748062384971295	27.046246122936818	29.582413714721472	22.623277777370415
42	20.771526408024695	27.037887064724043	30.334435653583032	21.856150873668234
43	21.330116806839765	26.08583432933223	30.051547525645443	22.532501338182563
44	21.07699865815118	26.749132931023105	29.62083605247142	22.55303235835429
45	21.43555826043599	26.70235153506038	28.959737202941803	22.902353001561824
46	22.210310971630527	26.835656516032525	28.518906869826	22.435125642510943
47	20.829013264505534	26.972774400750847	30.376524244935073	21.821688089808546
48	21.129646059877256	26.843135673380797	29.857822685310786	22.169395581431157
49	21.33627611289128	25.576078428497055	30.327983047243347	22.75966241136832
50	20.40959385242596	26.549688735069182	29.902111028824084	23.13860638368077
51	20.476612968272242	26.603655988092008	28.503948555129455	24.415782488506295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	348.0
1	636.0
2	924.0
3	5081.0
4	9238.0
5	7064.5
6	4891.0
7	3883.5
8	2876.0
9	2814.0
10	2752.0
11	2672.5
12	2593.0
13	2651.0
14	2709.0
15	2606.0
16	2503.0
17	2457.0
18	2411.0
19	2509.0
20	2607.0
21	2912.0
22	3217.0
23	3558.5
24	3900.0
25	5009.5
26	7149.0
27	8179.0
28	9642.0
29	11105.0
30	13165.0
31	15225.0
32	17661.0
33	20097.0
34	22656.5
35	25216.0
36	27308.0
37	29400.0
38	31137.0
39	32874.0
40	35145.5
41	37417.0
42	41302.5
43	45188.0
44	47467.5
45	49747.0
46	53195.0
47	56643.0
48	55860.5
49	55078.0
50	52927.5
51	50777.0
52	46299.5
53	41822.0
54	37675.0
55	33528.0
56	30798.0
57	28068.0
58	26088.5
59	24109.0
60	22322.0
61	20535.0
62	17977.5
63	15420.0
64	13470.0
65	11520.0
66	9744.5
67	7969.0
68	6685.0
69	5401.0
70	4520.5
71	3640.0
72	2981.5
73	2323.0
74	2005.5
75	1275.5
76	863.0
77	636.5
78	410.0
79	338.0
80	266.0
81	190.5
82	115.0
83	97.5
84	80.0
85	67.0
86	54.0
87	38.0
88	22.0
89	17.5
90	13.0
91	10.0
92	7.0
93	5.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	681895.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.106433179208274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16568447385701	29.394618172463417
2	8.348278404453806	6.529427831704004
3	3.7201689631672057	4.364476169203885
4	2.368250361924238	3.7045529812090408
5	1.608357599059672	3.144856448794945
6	1.223742364130172	2.871371939485375
7	0.9472454825300661	2.593037451680825
8	0.7187839685337984	2.2487261788602506
9	0.5713691570940626	2.0109788786303557
>10	5.254235299398508	39.45079714519148
>50	0.05376893055492683	1.3234977899576532
>100	0.017407207468201843	0.9743569729002686
>500	0.001934134163133537	0.5380822469502025
>1k	7.736536652534147E-4	0.8512197929684535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4327	0.6345551734504579	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1300	0.1906451873088965	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.12391937175078274	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	797	0.11688016483476195	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	784	0.11497371296167297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.933002881675331E-4	0.0	0.0	0.05646030547225012	0.0
2	2.933002881675331E-4	0.0	0.0	0.23874643456837197	0.0
3	2.933002881675331E-4	0.0	0.0	0.34624099018177285	0.0
4	2.933002881675331E-4	0.0	0.0	0.5091693002588376	0.0
5	2.933002881675331E-4	0.0	0.0	1.050308331927936	0.0
6	2.933002881675331E-4	0.0	0.0	1.4544761290227968	0.0
7	2.933002881675331E-4	0.0	0.0	1.832540200470747	0.0
8	2.933002881675331E-4	0.0	0.0	2.4800005866005765	0.0
9	2.933002881675331E-4	0.0	0.0	2.7952983963806743	0.0
10	2.933002881675331E-4	0.0	0.0	3.5779702153557364	0.0
11	2.933002881675331E-4	0.0	0.0	4.236722662580016	0.0
12	2.933002881675331E-4	0.0	0.0	4.94900241239487	0.0
13	2.933002881675331E-4	0.0	0.0	5.209746368575807	0.0
14	2.933002881675331E-4	0.0	0.0	5.342171448683448	0.0
15	2.933002881675331E-4	0.0	0.0	5.525924079220408	0.0
16	2.933002881675331E-4	0.0	0.0	5.875098072283856	0.0
17	2.933002881675331E-4	0.0	0.0	6.294810784651596	0.0
18	2.933002881675331E-4	0.0	0.0	6.794301175400904	0.0
19	2.933002881675331E-4	0.0	0.0	7.105786081434825	0.0
20	2.933002881675331E-4	0.0	0.0	7.412138232425813	0.0
21	2.933002881675331E-4	0.0	0.0	7.768498082549366	0.0
22	2.933002881675331E-4	0.0	0.0	8.195103351689044	0.0
23	2.933002881675331E-4	0.0	0.0	8.631680830626417	0.0
24	2.933002881675331E-4	0.0	0.0	8.965016608128817	0.0
25	2.933002881675331E-4	0.0	0.0	9.272395310128392	0.0
26	2.933002881675331E-4	0.0	0.0	9.56818865074535	0.0
27	2.933002881675331E-4	0.0	0.0	9.868381495684819	0.0
28	2.933002881675331E-4	0.0	0.0	10.180159702006907	0.0
29	2.933002881675331E-4	0.0	0.0	10.521854537722083	0.0
30	2.933002881675331E-4	0.0	0.0	10.918983127900923	0.0
31	2.933002881675331E-4	0.0	0.0	11.280329082923323	0.0
32	2.933002881675331E-4	0.0	0.0	11.611611758408552	0.0
33	2.933002881675331E-4	0.0	0.0	11.96738500795577	0.0
34	2.933002881675331E-4	0.0	0.0	12.322718307070737	0.0
35	2.933002881675331E-4	0.0	0.0	12.690076918000573	0.0
36	2.933002881675331E-4	0.0	0.0	13.063154884549673	0.0
37	2.933002881675331E-4	0.0	0.0	13.421861136978567	0.0
38	2.933002881675331E-4	0.0	0.0	13.823682531768087	0.0
39	2.933002881675331E-4	0.0	0.0	14.34238409139237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGC	20	7.031266E-4	45.0	15
TGCGGTC	20	7.031266E-4	45.0	15
ATCCGAC	20	7.031266E-4	45.0	43
CTCGAGT	20	7.031266E-4	45.0	26
CGACTAG	20	7.031266E-4	45.0	2
GTCGGCT	20	7.031266E-4	45.0	19
AACTCGA	30	2.164199E-6	44.999996	10
AATCGAG	30	2.164199E-6	44.999996	1
CACGACC	65	0.0	41.538464	27
CGTTTTT	1935	0.0	41.51163	1
CTAACGG	60	3.6379788E-12	41.249996	2
CGTCATA	55	6.002665E-11	40.909092	38
CGTTATT	385	0.0	40.324677	1
CGTTTTA	185	0.0	40.135136	1
CGAGTGA	40	3.4554614E-7	39.375	40
TCTCGTA	40	3.4554614E-7	39.375	28
CGATGCA	40	3.4554614E-7	39.375	10
TTTGACG	40	3.4554614E-7	39.375	1
ACGACCA	70	0.0	38.571426	28
GACTACG	35	6.2445524E-6	38.571426	1
>>END_MODULE