FastQCFastQC Report
Sat 14 Jan 2017
SRR2936053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769231
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.30809990757002775No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA253.889598E-545.026
CGATTAC207.03183E-445.05
TAACGCG207.03183E-445.01
TCTACGG453.8380676E-1045.02
TCTTCGA302.164561E-644.99999630
GTCTACG302.164561E-644.9999961
CGAATGG850.042.3529432
GCGAATG650.041.5384641
GACCTAT1250.041.39999833
TTGCACG501.0804797E-940.51
TTACGGG2450.039.4897963
TACGAAT1550.039.19354612
ACGTAAG750.039.0000041
TCGATCA1100.038.8636417
GCTTACG950.037.8947371
CCCCGAT301.1395709E-437.49999640
TATAGCG301.1395709E-437.4999961
TGGTCGA301.1395709E-437.4999961
CGTTTTT10000.037.351
AGGCGAT2050.037.3170747
Produced by FastQC (version 0.11.5)