##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	654003
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.775053019634466	31.0	31.0	34.0	30.0	34.0
2	31.88286598073709	33.0	31.0	34.0	30.0	34.0
3	31.589900963757046	33.0	31.0	34.0	30.0	34.0
4	35.464441294611795	37.0	35.0	37.0	33.0	37.0
5	31.053076209130538	37.0	35.0	37.0	0.0	37.0
6	33.3190077109738	37.0	35.0	37.0	17.0	37.0
7	29.62589315339532	35.0	32.0	37.0	0.0	37.0
8	32.6729326929693	35.0	33.0	37.0	17.0	37.0
9	36.50184020562597	39.0	35.0	39.0	32.0	39.0
10	36.74563725242851	38.0	35.0	39.0	33.0	39.0
11	36.896613624096524	39.0	37.0	39.0	33.0	39.0
12	36.987160609355	39.0	37.0	39.0	33.0	39.0
13	36.86462447419966	39.0	37.0	39.0	33.0	39.0
14	38.14396570046315	40.0	37.0	41.0	33.0	41.0
15	38.20710149647632	40.0	37.0	41.0	33.0	41.0
16	38.23969614818281	40.0	37.0	41.0	34.0	41.0
17	38.21249749618885	40.0	37.0	41.0	34.0	41.0
18	38.09992767617274	40.0	37.0	41.0	33.0	41.0
19	37.96279833578745	40.0	37.0	41.0	33.0	41.0
20	37.83403745854377	40.0	36.0	41.0	33.0	41.0
21	37.74592624192855	39.0	36.0	41.0	33.0	41.0
22	37.69963746343671	39.0	36.0	41.0	33.0	41.0
23	37.64347411250407	39.0	36.0	41.0	33.0	41.0
24	37.597751080652536	39.0	36.0	41.0	33.0	41.0
25	37.47047796416836	39.0	35.0	41.0	33.0	41.0
26	37.333877673344006	39.0	35.0	41.0	32.0	41.0
27	37.28457973434373	39.0	35.0	41.0	32.0	41.0
28	37.12487863205521	39.0	35.0	41.0	32.0	41.0
29	37.0899445415388	39.0	35.0	41.0	32.0	41.0
30	36.97149554359842	39.0	35.0	41.0	31.0	41.0
31	36.801779196731516	39.0	35.0	41.0	31.0	41.0
32	36.662669743105155	39.0	35.0	41.0	30.0	41.0
33	36.431788539196305	39.0	35.0	41.0	30.0	41.0
34	36.209443993376176	39.0	35.0	40.0	30.0	41.0
35	36.035450907717546	39.0	35.0	40.0	29.0	41.0
36	35.83205428721275	39.0	35.0	40.0	27.0	41.0
37	35.67986232479056	39.0	35.0	40.0	27.0	41.0
38	35.53200061773417	39.0	35.0	40.0	26.0	41.0
39	35.559592234286384	39.0	35.0	40.0	26.0	41.0
40	35.43598270955944	38.0	35.0	40.0	25.0	41.0
41	35.34227365929514	38.0	35.0	40.0	25.0	41.0
42	35.26894830757657	38.0	34.0	40.0	24.0	41.0
43	35.07911584503435	38.0	34.0	40.0	24.0	41.0
44	34.93243303165276	38.0	34.0	40.0	23.0	41.0
45	34.83208945524715	38.0	34.0	40.0	23.0	41.0
46	34.704635911456066	38.0	34.0	40.0	23.0	41.0
47	34.54461829685796	38.0	34.0	40.0	23.0	41.0
48	34.43968911457593	38.0	33.0	40.0	22.0	41.0
49	34.25467925988107	38.0	33.0	40.0	21.0	41.0
50	34.070961448189074	37.0	33.0	40.0	20.0	41.0
51	32.159464100317585	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	26.0
10	27.0
11	14.0
12	19.0
13	13.0
14	21.0
15	36.0
16	55.0
17	161.0
18	291.0
19	607.0
20	1087.0
21	1651.0
22	2658.0
23	3892.0
24	5725.0
25	7990.0
26	9937.0
27	10749.0
28	11305.0
29	12613.0
30	14904.0
31	17992.0
32	22913.0
33	30986.0
34	43659.0
35	53771.0
36	63725.0
37	98714.0
38	123028.0
39	115409.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34262381059414	20.906020308775343	23.497139921376508	14.25421595925401
2	33.86742874268161	24.766400154127734	24.751262608887114	16.614908494303542
3	31.28945891685512	24.15355892862877	28.97846034345408	15.578521811062028
4	27.337030564079985	25.70829185798842	28.869898150314295	18.084779427617303
5	21.531552607556844	38.148143051331566	23.919615047637397	16.400689293474187
6	24.23643316620872	35.2385233706879	27.106909295523106	13.41813416758027
7	68.4125302177513	19.020249142588032	9.019989204942485	3.547231434718189
8	82.3191942544606	4.8518126063641915	9.623503256101271	3.205489883073931
9	76.35010848574089	7.5322284454352655	11.398265757190716	4.719397311633127
10	37.37811600252598	30.01912835262224	19.632937463589617	12.969818181262166
11	27.12434040822443	27.009814939686823	28.691764410866615	17.174080241222136
12	25.54330790531542	23.80784186005263	31.97294202014364	18.675908214488313
13	22.841179627616388	24.896827690392858	33.60504462517756	18.656948056813192
14	19.792569758854317	28.267148621642406	31.742667847089383	20.197613772413888
15	19.247618130192063	27.59727401862071	35.078738170925824	18.076369680261408
16	21.57818847925774	26.074039415721334	33.64158880005137	18.70618330496955
17	21.865954743326867	25.68994331830282	31.185789667631493	21.25831227073882
18	22.51426981221799	26.322203414969042	32.07385898841443	19.089667784398543
19	23.204480713391224	27.930300013914312	29.896804754718246	18.968414517976218
20	24.592241931611937	26.739938501811157	30.350931111936795	18.316888454640115
21	23.873743698423404	27.463329678915844	30.47264309185126	18.19028353080949
22	21.849746866604587	25.145297498635326	30.652458780770118	22.35249685398997
23	20.809537570928573	27.719138903032555	30.623406926267922	20.84791659977095
24	20.640883910318454	26.35324302793718	32.164684259858134	20.84118880188623
25	20.731556277264783	27.35201520482322	30.374784213528073	21.541644304383926
26	20.51443189098521	28.91148817360165	29.81194275867236	20.76213717674078
27	21.395620509386042	28.116231882728364	30.224020379111412	20.26412722877418
28	19.59348810326558	27.500026758287042	32.6201867575531	20.286298380894277
29	21.611521659686574	26.401407944611876	31.611322883839982	20.375747511861565
30	22.48858185665815	26.24819763823713	31.03334388374365	20.229876621361065
31	23.049588457545305	26.61960266237311	29.321425131077376	21.00938374900421
32	23.08490939644008	27.81439840490028	29.75139257770989	19.34929962094975
33	22.747143361727698	27.060426328319593	29.74818158326491	20.444248726687796
34	21.889502035923382	26.577248116598856	30.604905482084945	20.92834436539282
35	22.4110592764865	26.402172467098772	29.877232979053613	21.309535277361114
36	22.571456094238098	28.486719479880062	29.23059985963367	19.711224566248166
37	21.24348053449296	28.768981182043508	30.741296293747887	19.246241989715642
38	21.268709776560655	28.592682296564387	29.459803701206265	20.67880422566869
39	22.296380903451514	27.50400227521892	28.838705632848775	21.360911188480785
40	22.49714450851143	26.717002827204155	30.030749094423115	20.755103569861298
41	20.305717252061534	28.1503295856441	29.25521748371185	22.288735678582512
42	21.99424161662867	27.744979763089773	29.387938587437674	20.872840032843886
43	21.99072481318893	27.18198540373668	29.58824347900545	21.239046304068943
44	21.177884505116946	27.074034828586413	29.539161135346475	22.208919530950162
45	21.84240745073035	26.81394427854307	28.858736122005556	22.48491214872103
46	22.111519366119115	27.674337885300222	28.47051160315778	21.74363114542288
47	21.33782260937641	26.663180444126404	30.57004325668231	21.42895368981488
48	21.43292920674676	26.46639235599837	29.89741637270777	22.203262064547104
49	21.437516341668157	26.463792979542905	29.934725070068485	22.16396560872045
50	20.445930676158977	26.28718828506903	30.448025467773082	22.81885557099891
51	20.65510402857479	26.16853439510216	28.698033495259196	24.47832808106385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	665.0
2	1029.0
3	5256.0
4	9483.0
5	7474.5
6	5466.0
7	4381.0
8	3296.0
9	3120.0
10	2944.0
11	2929.0
12	2914.0
13	2868.5
14	2823.0
15	2669.5
16	2516.0
17	2599.5
18	2683.0
19	2750.0
20	2817.0
21	2971.5
22	3126.0
23	3600.5
24	4075.0
25	4939.0
26	7137.5
27	8472.0
28	9821.5
29	11171.0
30	12699.0
31	14227.0
32	15814.0
33	17401.0
34	19442.0
35	21483.0
36	23547.0
37	25611.0
38	27400.5
39	29190.0
40	32532.0
41	35874.0
42	38870.0
43	41866.0
44	45046.5
45	48227.0
46	52972.5
47	57718.0
48	56436.0
49	55154.0
50	52815.5
51	50477.0
52	45950.5
53	41424.0
54	36548.5
55	31673.0
56	28942.5
57	26212.0
58	24690.0
59	23168.0
60	21387.0
61	19606.0
62	17238.5
63	14871.0
64	12807.5
65	10744.0
66	8715.0
67	6686.0
68	5496.5
69	4307.0
70	3761.5
71	3216.0
72	2729.0
73	2242.0
74	1826.5
75	1158.0
76	905.0
77	751.5
78	598.0
79	457.0
80	316.0
81	282.5
82	249.0
83	185.5
84	122.0
85	89.5
86	57.0
87	37.5
88	18.0
89	16.5
90	15.0
91	12.0
92	9.0
93	6.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	654003.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.416441037130184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99906851680876	31.47610940103227
2	8.511116844735914	7.05000337920261
3	3.7121333246028385	4.612300528811384
4	2.2679631193714513	3.7572384323133425
5	1.540683459136356	3.190481282110133
6	1.117411308872851	2.776751975289294
7	0.8766231336464709	2.541462722851735
8	0.6624784010863092	2.1949998109570723
9	0.5609578724368434	2.0909590783285066
>10	4.676903779934552	36.32190955082823
>50	0.055995179734426324	1.4755604908943327
>100	0.016379542127218386	1.1936373930096433
>500	0.0015236783374156632	0.419334944570398
>1k	7.618391687078316E-4	0.8992510098010793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4297	0.6570306252417802	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1403	0.21452500982411396	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	854	0.13058044076250414	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0580899475996285E-4	0.0	0.0	0.08012195662711027	0.0
2	3.0580899475996285E-4	0.0	0.0	0.3203349220110611	0.0
3	3.0580899475996285E-4	0.0	0.0	0.49464604902424	0.0
4	3.0580899475996285E-4	0.0	0.0	0.6857766707492168	0.0
5	3.0580899475996285E-4	0.0	0.0	1.300605654714122	0.0
6	3.0580899475996285E-4	0.0	0.0	1.8350068730571572	0.0
7	3.0580899475996285E-4	0.0	0.0	2.30350625302942	0.0
8	3.0580899475996285E-4	0.0	0.0	3.113900089143322	0.0
9	3.0580899475996285E-4	0.0	0.0	3.4970787595775557	0.0
10	3.0580899475996285E-4	0.0	0.0	4.358389793318991	0.0
11	3.0580899475996285E-4	0.0	0.0	5.280098103525519	0.0
12	3.0580899475996285E-4	0.0	0.0	6.09339712508964	0.0
13	3.0580899475996285E-4	0.0	0.0	6.456851115361856	0.0
14	3.0580899475996285E-4	0.0	0.0	6.6277983434326755	0.0
15	3.0580899475996285E-4	0.0	0.0	6.858531229979067	0.0
16	3.0580899475996285E-4	0.0	0.0	7.33192355386749	0.0
17	3.0580899475996285E-4	0.0	0.0	7.90745608200574	0.0
18	3.0580899475996285E-4	0.0	0.0	8.56051119031564	0.0
19	3.0580899475996285E-4	0.0	0.0	8.943384051755114	0.0
20	3.0580899475996285E-4	0.0	0.0	9.323657536739129	0.0
21	3.0580899475996285E-4	0.0	0.0	9.80607122597297	0.0
22	3.0580899475996285E-4	0.0	0.0	10.30270503346315	0.0
23	3.0580899475996285E-4	0.0	0.0	10.837412060800945	0.0
24	3.0580899475996285E-4	0.0	0.0	11.251477439705933	0.0
25	3.0580899475996285E-4	0.0	0.0	11.61798951992575	0.0
26	3.0580899475996285E-4	0.0	0.0	11.966764678449488	0.0
27	3.0580899475996285E-4	0.0	0.0	12.330524477716462	0.0
28	3.0580899475996285E-4	0.0	0.0	12.6831222486747	0.0
29	3.0580899475996285E-4	0.0	0.0	13.077615851915052	0.0
30	3.0580899475996285E-4	0.0	0.0	13.5080420120397	0.0
31	3.0580899475996285E-4	0.0	0.0	13.928223570839888	0.0
32	3.0580899475996285E-4	0.0	0.0	14.33433791588112	0.0
33	3.0580899475996285E-4	0.0	0.0	14.733571558540252	0.0
34	3.0580899475996285E-4	0.0	0.0	15.131276156225583	0.0
35	3.0580899475996285E-4	0.0	0.0	15.519347770575976	0.0
36	3.0580899475996285E-4	0.0	0.0	15.923780166146027	0.0
37	3.0580899475996285E-4	0.0	0.0	16.32897708420298	0.0
38	3.0580899475996285E-4	0.0	0.0	16.72836363135949	0.0
39	3.0580899475996285E-4	0.0	0.0	17.14319353275138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	70	0.0	45.000004	2
CGACGGT	20	7.0310547E-4	45.0	28
CACGCGA	20	7.0310547E-4	45.0	1
CGTTCAA	20	7.0310547E-4	45.0	9
CCGTACA	20	7.0310547E-4	45.0	28
GTCAACG	20	7.0310547E-4	45.0	1
CGTACAT	20	7.0310547E-4	45.0	29
ACCGTAC	20	7.0310547E-4	45.0	27
CGCACGA	20	7.0310547E-4	45.0	39
TTTGACG	20	7.0310547E-4	45.0	1
TATCGAG	20	7.0310547E-4	45.0	1
TGCCGTA	20	7.0310547E-4	45.0	45
CGTTTTA	235	0.0	42.12766	1
CGTTTTT	1855	0.0	41.48248	1
TACGGGT	60	3.6379788E-12	41.249996	4
CGGTTTT	535	0.0	39.95327	1
GCTACGA	80	0.0	39.375	10
CGTTATT	390	0.0	39.230766	1
AGGCGAT	115	0.0	39.130432	7
CGTTGAT	35	6.2441595E-6	38.57143	25
>>END_MODULE