FastQCFastQC Report
Sat 14 Jan 2017
SRR2936051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878263
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61570.7010428539059484No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.21588066444789317No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.1424402485360308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC207.032376E-445.00000428
CGCCTAC207.032376E-445.00000430
CCCCGAT406.8121153E-945.00000440
TGCGACG207.032376E-445.0000041
ACGTCGC207.032376E-445.00000420
CGTAACA207.032376E-445.00000412
CGGCTAA207.032376E-445.00000429
CAATCGA207.032376E-445.00000434
TAGTACG302.164914E-645.0000041
CGTTATT5950.041.5966381
CGTTTTT27500.041.318181
CGGTTTT7050.041.170211
AATTGCG556.002665E-1140.9090921
ATTTACG403.4569712E-739.3750041
ACGTACG403.4569712E-739.3750041
GTAACGG1150.039.1304362
ACAACGA750.039.013
CTATGCG356.246608E-638.5714261
TCGACGT356.246608E-638.57142626
TAGGCGA1350.038.3333326
Produced by FastQC (version 0.11.5)