##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878263
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875846984331574	31.0	31.0	34.0	30.0	34.0
2	32.004703602451656	33.0	31.0	34.0	30.0	34.0
3	31.692399657050338	33.0	31.0	34.0	30.0	34.0
4	35.55168440432991	37.0	35.0	37.0	33.0	37.0
5	30.99312620479287	37.0	35.0	37.0	0.0	37.0
6	33.32941499300323	37.0	35.0	37.0	17.0	37.0
7	29.662153591805644	35.0	32.0	37.0	0.0	37.0
8	32.73296609330007	35.0	35.0	37.0	17.0	37.0
9	36.589843816715494	39.0	35.0	39.0	32.0	39.0
10	36.918191931118585	38.0	37.0	39.0	34.0	39.0
11	37.0388926779336	39.0	37.0	39.0	34.0	39.0
12	37.13740303303225	39.0	37.0	39.0	34.0	39.0
13	37.039871883479094	39.0	37.0	39.0	33.0	39.0
14	38.34390495785431	40.0	38.0	41.0	34.0	41.0
15	38.431666824174535	40.0	38.0	41.0	34.0	41.0
16	38.43913041993116	40.0	38.0	41.0	34.0	41.0
17	38.394322657336126	40.0	38.0	41.0	34.0	41.0
18	38.2590010053936	40.0	37.0	41.0	34.0	41.0
19	38.1471222173768	40.0	37.0	41.0	34.0	41.0
20	38.01396620374535	40.0	37.0	41.0	34.0	41.0
21	37.91921895833025	40.0	36.0	41.0	33.0	41.0
22	37.883551965641274	40.0	36.0	41.0	33.0	41.0
23	37.805731312829984	40.0	36.0	41.0	33.0	41.0
24	37.76229102216534	40.0	36.0	41.0	33.0	41.0
25	37.64427398171163	39.0	36.0	41.0	33.0	41.0
26	37.47292212013941	39.0	35.0	41.0	33.0	41.0
27	37.412552959648764	39.0	35.0	41.0	33.0	41.0
28	37.30054436996663	39.0	35.0	41.0	32.0	41.0
29	37.25712685152397	39.0	35.0	41.0	32.0	41.0
30	37.09521066013256	39.0	35.0	41.0	32.0	41.0
31	36.93710084564646	39.0	35.0	41.0	31.0	41.0
32	36.76727358433636	39.0	35.0	41.0	31.0	41.0
33	36.50632327674057	39.0	35.0	41.0	30.0	41.0
34	36.28150451516232	39.0	35.0	41.0	30.0	41.0
35	36.046922163406634	39.0	35.0	41.0	28.0	41.0
36	35.8134009971956	39.0	35.0	41.0	27.0	41.0
37	35.6622435420825	39.0	35.0	40.0	26.0	41.0
38	35.528954310952415	39.0	35.0	40.0	25.0	41.0
39	35.464232240228725	39.0	35.0	40.0	25.0	41.0
40	35.3383405654115	39.0	35.0	40.0	24.0	41.0
41	35.17449556681768	38.0	34.0	40.0	24.0	41.0
42	35.064816575444944	38.0	34.0	40.0	23.0	41.0
43	34.86380275612203	38.0	34.0	40.0	23.0	41.0
44	34.70446210303747	38.0	34.0	40.0	22.0	41.0
45	34.582293686515314	38.0	34.0	40.0	22.0	41.0
46	34.46548926688247	38.0	34.0	40.0	21.0	41.0
47	34.31092622597104	38.0	33.0	40.0	20.0	41.0
48	34.20361212985176	38.0	33.0	40.0	20.0	41.0
49	34.0313823991219	38.0	33.0	40.0	20.0	41.0
50	33.88067924983746	38.0	33.0	40.0	19.0	41.0
51	31.96320350510041	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	18.0
9	24.0
10	38.0
11	28.0
12	29.0
13	28.0
14	34.0
15	51.0
16	103.0
17	193.0
18	390.0
19	736.0
20	1311.0
21	2181.0
22	3523.0
23	5302.0
24	7963.0
25	11281.0
26	13949.0
27	15322.0
28	15953.0
29	17097.0
30	19197.0
31	23401.0
32	29339.0
33	39785.0
34	55885.0
35	69120.0
36	82577.0
37	131730.0
38	167768.0
39	163893.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.57550300991844	20.62195492694102	24.780390384201542	15.022151678938997
2	34.62117839417122	23.686185117669766	25.422111599828295	16.27052488833072
3	31.767363534613207	23.68561581212006	28.784316315272306	15.762704337994426
4	27.709581298540414	25.641863542014182	29.224617227413656	17.42393793203175
5	21.11725075518381	39.25464240210507	23.702011811951547	15.92609503075958
6	24.816256633832918	34.67082183810544	26.95286036187338	13.56006116618826
7	68.3972796303613	18.86052355615573	9.046720629242037	3.6954761842409396
8	83.05792228523802	4.334123149899289	9.600882651324262	3.0070719135384274
9	77.04377845816116	7.254888342102536	11.436096021351236	4.265237178385062
10	36.929256953782634	32.29898105692714	18.180658868698785	12.591103120591441
11	26.74153414182312	26.770568724858045	29.585784668146104	16.902112465172735
12	25.69469509702674	23.80949669973573	32.04518464286893	18.45062356036859
13	22.60803426763965	23.587012090911262	33.84111592996631	19.96383771148278
14	18.918592722225576	27.89118976889611	32.77617296868933	20.414044540188986
15	19.09803783149239	27.21781516470579	35.089716861577905	18.59443014222391
16	22.15759971671356	25.96716473311525	33.81720509687873	18.058030453292464
17	22.180485799811674	24.778113162002725	31.875190005727216	21.16621103245839
18	22.285124159847335	26.326396534978702	31.562299675609694	19.826179629564265
19	23.140904262162927	27.410354301615804	29.907556164839004	19.541185271382265
20	24.39872794367974	26.966523695066286	30.193347550790595	18.441400810463378
21	23.656011923535434	26.26605014671004	31.415760427115796	18.66217750263873
22	22.423351547315555	24.89129110528395	31.227548012383533	21.457809335016957
23	21.543546750802438	26.65796008712652	31.376136760856372	20.42235640121467
24	21.9381893578575	25.373265183663662	32.36650069512208	20.32204476335676
25	21.979293218546154	26.96094450067918	30.087570579655527	20.97219170111914
26	21.165414004688802	27.609383521792445	30.76219765605519	20.463004817463563
27	21.206517865377457	27.208478553690636	31.14886998541439	20.436133595517518
28	19.932298184029158	27.829021602868387	31.270815234161063	20.96786497894139
29	21.406571835543566	26.416916117381696	31.322052733634457	20.85445931344028
30	21.821937164607867	25.21738932415461	31.877694950145912	21.082978561091608
31	21.89162016389168	26.529410894003274	30.059219163280247	21.519749778824792
32	22.436673297178636	26.827954724268245	30.357535271325332	20.37783670722779
33	21.979862524095857	26.474415977901838	30.325995743871708	21.219725754130597
34	21.377309530288763	25.80901165140738	31.47234939875641	21.341329419547446
35	21.25422567044268	25.49054212690276	31.63881434148996	21.616417861164592
36	21.4498390573211	27.671210104490342	29.955491692124113	20.923459146064445
37	21.52897252873	27.548581689084024	30.56464863030778	20.357797151878195
38	21.539106167514742	26.63461855958864	30.279084966576068	21.547190306320545
39	22.27009449333514	25.773145401776006	29.743026860974446	22.213733243914408
40	22.35674279800014	25.350606822785434	31.60180948075918	20.690840898455246
41	20.659642954331446	26.401089423099915	31.450715787867644	21.488551834700996
42	21.933748774569803	26.68221250354393	29.962664942050388	21.421373779835882
43	22.25574799348259	26.083986231914587	30.118654662669382	21.54161111193344
44	21.504150806762894	26.948078195255864	29.043691923717613	22.50407907426363
45	21.343948225076087	26.070436759831622	29.542631307478512	23.04298370761378
46	21.476596418157204	26.3339113682348	29.44846816955741	22.741024044050587
47	21.68257116604024	26.35634200689315	30.2724810221995	21.688605804867105
48	20.80367725840665	25.718947513444153	30.759920433856376	22.717454794292827
49	20.8960186185687	25.601215125765286	30.65448504605113	22.84828120961489
50	19.65470479799331	25.68638323600106	31.497284981833463	23.161626984172166
51	19.906451712072577	25.430195738634097	30.696272073399427	23.967080475893894
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	416.0
1	873.5
2	1331.0
3	7685.0
4	14039.0
5	10943.5
6	7848.0
7	6211.5
8	4575.0
9	4333.5
10	4092.0
11	4026.5
12	3961.0
13	3843.0
14	3725.0
15	3659.5
16	3594.0
17	3427.0
18	3260.0
19	3427.5
20	3595.0
21	4052.0
22	4509.0
23	5063.0
24	5617.0
25	6700.0
26	9064.0
27	10345.0
28	12397.5
29	14450.0
30	17456.5
31	20463.0
32	22900.5
33	25338.0
34	27864.0
35	30390.0
36	33093.5
37	35797.0
38	38829.0
39	41861.0
40	44239.5
41	46618.0
42	50170.5
43	53723.0
44	57445.5
45	61168.0
46	66436.5
47	71705.0
48	70627.5
49	69550.0
50	65890.0
51	62230.0
52	56239.5
53	50249.0
54	46366.5
55	42484.0
56	39520.5
57	36557.0
58	34798.5
59	33040.0
60	32320.0
61	31600.0
62	28004.0
63	24408.0
64	20219.0
65	16030.0
66	13232.5
67	10435.0
68	8864.5
69	7294.0
70	6055.5
71	4817.0
72	4221.5
73	3626.0
74	2996.5
75	1925.5
76	1484.0
77	1174.0
78	864.0
79	626.0
80	388.0
81	322.5
82	257.0
83	213.0
84	169.0
85	126.0
86	83.0
87	65.5
88	48.0
89	45.0
90	42.0
91	33.5
92	25.0
93	17.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	878263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.40856898860248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34048039225725	28.55311469791862
2	8.43668386785611	6.480819087471619
3	3.8923353078179916	4.484970875913017
4	2.458762861708024	3.7775025200210517
5	1.724674338608102	3.312113665865082
6	1.3402571728080162	3.0886416050559604
7	0.9589198957697477	2.57815186798511
8	0.766317291193908	2.3546520436784153
9	0.6316718956992582	2.1835452225713645
>10	5.366147478661909	39.14872970541732
>50	0.0629628210836775	1.5358005509471406
>100	0.01958165180934283	1.3292556680221215
>500	3.012561816730348E-4	0.09615350453573358
>1k	6.025123633460696E-4	0.3641336688013882
>5k	3.012561816730348E-4	0.712415315796043
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6157	0.7010428539059484	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1896	0.21588066444789317	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1251	0.1424402485360308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2772221988174386E-4	0.0	0.0	0.06729191597505531	0.0
2	2.2772221988174386E-4	0.0	0.0	0.2474201919015147	0.0
3	2.2772221988174386E-4	0.0	0.0	0.41866730125258605	0.0
4	3.415833298226158E-4	0.0	0.0	0.5839936328867321	0.0
5	3.415833298226158E-4	0.0	0.0	1.0965963498405376	0.0
6	3.415833298226158E-4	0.0	0.0	1.6229762610971885	0.0
7	3.415833298226158E-4	0.0	0.0	2.0595197566104915	0.0
8	3.415833298226158E-4	0.0	0.0	2.764889332694193	0.0
9	3.415833298226158E-4	0.0	0.0	3.13664585665114	0.0
10	3.415833298226158E-4	0.0	0.0	3.8002284053865414	0.0
11	3.415833298226158E-4	0.0	0.0	4.519944481322793	0.0
12	3.415833298226158E-4	0.0	0.0	5.1076955308375735	0.0
13	3.415833298226158E-4	0.0	0.0	5.390071083490936	0.0
14	3.415833298226158E-4	0.0	0.0	5.532397470917026	0.0
15	3.415833298226158E-4	0.0	0.0	5.7266445244761535	0.0
16	3.415833298226158E-4	0.0	0.0	6.115594076034172	1.1386110994087193E-4
17	3.415833298226158E-4	0.0	0.0	6.571038515797659	1.1386110994087193E-4
18	3.415833298226158E-4	0.0	0.0	7.103339204771236	1.1386110994087193E-4
19	3.415833298226158E-4	0.0	0.0	7.418392895977629	1.1386110994087193E-4
20	3.415833298226158E-4	0.0	0.0	7.724907003938456	1.1386110994087193E-4
21	4.554444397634877E-4	0.0	0.0	8.11453972215612	1.1386110994087193E-4
22	4.554444397634877E-4	0.0	0.0	8.538444634465986	1.1386110994087193E-4
23	4.554444397634877E-4	0.0	0.0	8.96075549123668	1.1386110994087193E-4
24	4.554444397634877E-4	0.0	0.0	9.29505171002308	1.1386110994087193E-4
25	4.554444397634877E-4	0.0	0.0	9.598947012455266	1.1386110994087193E-4
26	4.554444397634877E-4	0.0	0.0	9.893050259432538	1.1386110994087193E-4
27	4.554444397634877E-4	0.0	0.0	10.193302006346618	1.1386110994087193E-4
28	4.554444397634877E-4	0.0	0.0	10.50311808649573	1.1386110994087193E-4
29	4.554444397634877E-4	0.0	0.0	10.810087638896322	1.1386110994087193E-4
30	4.554444397634877E-4	0.0	0.0	11.196076801595877	1.1386110994087193E-4
31	5.693055497043596E-4	0.0	0.0	11.554739297909624	1.1386110994087193E-4
32	5.693055497043596E-4	0.0	0.0	11.892792933324072	1.1386110994087193E-4
33	5.693055497043596E-4	0.0	0.0	12.221054513283606	1.1386110994087193E-4
34	5.693055497043596E-4	0.0	0.0	12.558197259818527	1.1386110994087193E-4
35	5.693055497043596E-4	0.0	0.0	12.902399395169784	1.1386110994087193E-4
36	5.693055497043596E-4	0.0	0.0	13.252750030457847	1.1386110994087193E-4
37	5.693055497043596E-4	0.0	0.0	13.606288776824254	1.1386110994087193E-4
38	5.693055497043596E-4	0.0	0.0	13.95811960654155	1.1386110994087193E-4
39	5.693055497043596E-4	0.0	0.0	14.318034575064644	1.1386110994087193E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAC	20	7.032376E-4	45.000004	28
CGCCTAC	20	7.032376E-4	45.000004	30
CCCCGAT	40	6.8121153E-9	45.000004	40
TGCGACG	20	7.032376E-4	45.000004	1
ACGTCGC	20	7.032376E-4	45.000004	20
CGTAACA	20	7.032376E-4	45.000004	12
CGGCTAA	20	7.032376E-4	45.000004	29
CAATCGA	20	7.032376E-4	45.000004	34
TAGTACG	30	2.164914E-6	45.000004	1
CGTTATT	595	0.0	41.596638	1
CGTTTTT	2750	0.0	41.31818	1
CGGTTTT	705	0.0	41.17021	1
AATTGCG	55	6.002665E-11	40.909092	1
ATTTACG	40	3.4569712E-7	39.375004	1
ACGTACG	40	3.4569712E-7	39.375004	1
GTAACGG	115	0.0	39.130436	2
ACAACGA	75	0.0	39.0	13
CTATGCG	35	6.246608E-6	38.571426	1
TCGACGT	35	6.246608E-6	38.571426	26
TAGGCGA	135	0.0	38.333332	6
>>END_MODULE