##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	436565
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848311248038666	31.0	31.0	34.0	30.0	34.0
2	31.994894231099607	33.0	31.0	34.0	30.0	34.0
3	31.930848785404237	33.0	31.0	34.0	30.0	34.0
4	35.651513520323434	37.0	35.0	37.0	33.0	37.0
5	31.05060414829407	37.0	35.0	37.0	0.0	37.0
6	33.32793742054448	37.0	35.0	37.0	17.0	37.0
7	29.65950545737748	35.0	32.0	37.0	0.0	37.0
8	32.7056566605202	35.0	35.0	37.0	17.0	37.0
9	36.536495138181024	39.0	35.0	39.0	32.0	39.0
10	36.78176903782942	38.0	35.0	39.0	33.0	39.0
11	36.89144113705863	39.0	37.0	39.0	33.0	39.0
12	36.93910643317719	39.0	37.0	39.0	33.0	39.0
13	36.742526313378306	39.0	35.0	39.0	33.0	39.0
14	37.96362053760608	40.0	37.0	41.0	33.0	41.0
15	38.100532566742636	40.0	37.0	41.0	33.0	41.0
16	38.148168084935804	40.0	37.0	41.0	33.0	41.0
17	38.11523599005876	40.0	37.0	41.0	33.0	41.0
18	38.044007192514286	40.0	37.0	41.0	33.0	41.0
19	37.96392976990826	40.0	37.0	41.0	33.0	41.0
20	37.90787397065729	40.0	36.0	41.0	33.0	41.0
21	37.83555026170215	40.0	36.0	41.0	33.0	41.0
22	37.82080789802206	40.0	36.0	41.0	33.0	41.0
23	37.778715655171624	40.0	36.0	41.0	33.0	41.0
24	37.71929953157033	40.0	36.0	41.0	33.0	41.0
25	37.60349547031942	39.0	36.0	41.0	33.0	41.0
26	37.40770332023868	39.0	36.0	41.0	32.0	41.0
27	37.390370277049236	39.0	36.0	41.0	32.0	41.0
28	37.30983244190441	39.0	36.0	41.0	32.0	41.0
29	37.33820164236712	39.0	36.0	41.0	32.0	41.0
30	37.230210850617894	39.0	36.0	41.0	32.0	41.0
31	37.08877944865026	39.0	35.0	41.0	31.0	41.0
32	36.98861337945094	39.0	35.0	41.0	31.0	41.0
33	36.829640488816096	39.0	35.0	41.0	31.0	41.0
34	36.71813361126064	39.0	35.0	41.0	30.0	41.0
35	36.64975204150585	39.0	35.0	41.0	30.0	41.0
36	36.5091910712036	39.0	35.0	40.0	30.0	41.0
37	36.389282237467505	39.0	35.0	40.0	30.0	41.0
38	36.29989577726112	39.0	35.0	40.0	30.0	41.0
39	36.3343763242587	39.0	35.0	40.0	30.0	41.0
40	36.21834320204322	39.0	35.0	40.0	30.0	41.0
41	36.15479939986027	39.0	35.0	40.0	30.0	41.0
42	36.080169046991855	39.0	35.0	40.0	29.0	41.0
43	35.906627879009996	38.0	35.0	40.0	29.0	41.0
44	35.691693104119665	38.0	35.0	40.0	28.0	41.0
45	35.61206005978491	38.0	35.0	40.0	27.0	41.0
46	35.49935977460401	38.0	34.0	40.0	27.0	41.0
47	35.34149095781842	38.0	34.0	40.0	26.0	41.0
48	35.27193430531536	38.0	34.0	40.0	26.0	41.0
49	35.10726695910117	38.0	34.0	40.0	26.0	41.0
50	34.95793524446531	38.0	34.0	40.0	24.0	41.0
51	32.98710615830403	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	16.0
10	15.0
11	9.0
12	12.0
13	8.0
14	11.0
15	21.0
16	40.0
17	90.0
18	181.0
19	287.0
20	519.0
21	845.0
22	1284.0
23	1807.0
24	2629.0
25	3463.0
26	4274.0
27	5155.0
28	5851.0
29	7293.0
30	9187.0
31	11735.0
32	15340.0
33	21393.0
34	30863.0
35	38490.0
36	42941.0
37	67198.0
38	84067.0
39	81521.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.75834068237261	22.458282271826647	26.530299039089257	11.253078006711487
2	30.184279546001168	26.317501402998406	25.90885664219532	17.589362408805105
3	32.61621980690161	24.47218627237639	26.42928315371136	16.48231076701064
4	28.088142659168735	27.4724267863892	26.02086745387285	18.418563100569216
5	21.565402631910484	40.2943433394798	21.555094888504573	16.585159140105137
6	25.833953706779063	36.8836255769473	23.6258060082691	13.656614708004536
7	70.90719595020215	19.33022573958059	6.136543240983587	3.626035069233677
8	85.7617995029377	5.124322838523473	5.814254463825548	3.2996231947132726
9	79.85546253135271	8.048515112297137	7.596577829189238	4.499444527160904
10	37.16537056337545	37.3809169310412	14.489251314237283	10.964461191346077
11	27.099057414130773	27.19938611661494	28.706378202558614	16.99517826669568
12	26.963682384066516	24.939470640110866	30.104795391293393	17.99205158452922
13	23.34795505823875	27.89916736339377	30.649960486983613	18.102917091383873
14	18.925016893246138	31.29247649261851	30.01110945678192	19.77139715735343
15	18.207368891230402	28.623687194346775	34.79894173834366	18.370002176079165
16	21.792402047805023	27.466700262274806	32.33584918626092	18.40504850365925
17	21.177144296954634	27.232829017442995	29.145717132614845	22.444309552987527
18	21.869366531902465	26.758672820771253	30.929643924730566	20.44231672259572
19	22.30549860845464	29.312244453861396	28.191449154192387	20.190807783491575
20	24.345057437036868	28.0847067447001	29.707603678719092	17.86263213954394
21	22.757894013491693	28.267268333466955	29.54313790615372	19.431699746887634
22	21.642367116007925	26.587564280233185	29.503968481211274	22.266100122547616
23	20.476217745352926	27.53404418586007	29.67965824104085	22.310079827746154
24	20.642974127564052	27.856562023982683	30.825192124884037	20.675271723569228
25	20.556618143919003	29.22795001889753	29.080434757710766	21.1349970794727
26	19.143082931522223	31.65233126796697	28.434253776642652	20.77033202386815
27	20.17660600368788	30.28483730944991	29.276052821458432	20.262503865403776
28	18.01427049809307	29.995533311190776	31.27598410316906	20.7142120875471
29	18.8650029205273	28.22420487212672	31.276900347027357	21.633891860318624
30	20.769873901939	29.52298054127106	29.813887966282227	19.893257590507712
31	22.019630524664137	30.54871554064114	26.51243228385235	20.91922165084237
32	21.619461019550354	30.69279488735927	26.494336467650864	21.193407625439512
33	21.026193121299233	30.633697158498734	26.714464054608133	21.625645665593897
34	19.448650258266238	29.708061800648245	27.705839909291857	23.13744803179366
35	19.262423694066175	29.08936813532922	27.611695852851238	24.03651231775337
36	21.57891722882045	30.488701567922305	27.613299279603265	20.319081923653982
37	20.17087947957349	30.47449978811861	28.669728448226493	20.684892284081407
38	19.595020214630125	30.705622301375513	26.39813086252906	23.301226621465304
39	21.25342159815835	28.30597963648025	27.97613184749121	22.46446691787019
40	20.50966064618098	28.083561439877226	28.569857867671484	22.836920046270315
41	19.372831078991673	28.203589385314903	28.12799926700491	24.29558026868851
42	21.2302864407362	28.839233562012527	27.58100168359809	22.34947831365318
43	21.830655228889167	28.744860444607333	27.297424209453347	22.127060117050153
44	19.364813945231525	29.4489938497131	27.581230744562667	23.60496146049271
45	19.424598856985785	30.260785908169456	26.65193041127896	23.66268482356579
46	21.482253501769495	29.641405059956703	26.972386700720396	21.9039547375534
47	19.748720121860433	28.90611936366864	28.149301936710458	23.195858577760468
48	20.302131412275376	27.732181920218068	28.703400410019125	23.262286257487432
49	20.937088406079276	26.66636125204723	29.241693676772073	23.154856665101416
50	18.95639824539301	27.475404578928682	29.06829452658825	24.499902649090053
51	18.76902637637007	27.837320902958325	27.463035286841592	25.930617433830015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	324.0
1	583.0
2	842.0
3	1334.5
4	1827.0
5	1460.5
6	1094.0
7	989.5
8	885.0
9	967.0
10	1049.0
11	1053.5
12	1058.0
13	1093.0
14	1128.0
15	1208.5
16	1289.0
17	1280.5
18	1272.0
19	1501.0
20	1730.0
21	1921.0
22	2112.0
23	2220.5
24	2329.0
25	2969.0
26	4659.0
27	5709.0
28	6786.0
29	7863.0
30	9480.5
31	11098.0
32	12663.0
33	14228.0
34	15717.5
35	17207.0
36	18843.0
37	20479.0
38	21805.0
39	23131.0
40	25238.0
41	27345.0
42	29697.5
43	32050.0
44	33981.5
45	35913.0
46	38455.5
47	40998.0
48	40245.0
49	39492.0
50	37153.5
51	34815.0
52	30489.5
53	26164.0
54	23018.5
55	19873.0
56	17556.0
57	15239.0
58	14201.5
59	13164.0
60	11767.5
61	10371.0
62	8744.5
63	7118.0
64	6012.5
65	4907.0
66	4098.0
67	3289.0
68	2584.5
69	1880.0
70	1607.5
71	1335.0
72	1136.5
73	938.0
74	796.0
75	484.0
76	314.0
77	261.5
78	209.0
79	147.0
80	85.0
81	70.5
82	56.0
83	43.0
84	30.0
85	26.5
86	23.0
87	25.5
88	28.0
89	17.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	436565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.10313525363831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37326629406114	27.957514896038315
2	9.545517925544242	7.274283211660823
3	4.129486792389774	4.720391813356216
4	2.4744001270365032	3.771296108483667
5	1.7154830559989616	3.268264145402661
6	1.2792333627568728	2.924568110525501
7	0.9580614336459754	2.5553601071255057
8	0.7167982971913802	2.184980997396863
9	0.5438343411494456	1.8649614110751542
>10	5.141258024457357	39.3577331282823
>50	0.10052164921503885	2.4168954849503397
>100	0.021540353403222613	1.5411954983741436
>500	5.983431500895171E-4	0.16255508732851545
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	713	0.16332046774248965	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	468	0.10720053142143783	No Hit
TAGGGGAGGAGCTTTGACCAATTTGACATAGAGCTGGAATGTAACGTTGCT	454	0.10399367791737771	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2906096457572182E-4	0.0	0.0	0.10765865335058926	0.0
2	2.2906096457572182E-4	0.0	0.0	0.48950328129831755	0.0
3	2.2906096457572182E-4	0.0	0.0	0.657863090261473	0.0
4	2.2906096457572182E-4	0.0	0.0	0.9052489320032526	0.0
5	2.2906096457572182E-4	0.0	0.0	1.6070917274632643	0.0
6	2.2906096457572182E-4	0.0	0.0	2.0251279878139568	0.0
7	2.2906096457572182E-4	0.0	0.0	2.4807302463550673	0.0
8	2.2906096457572182E-4	0.0	0.0	3.294583853492607	0.0
9	2.2906096457572182E-4	0.0	0.0	3.635426568781281	0.0
10	2.2906096457572182E-4	0.0	0.0	4.49325988111736	0.0
11	2.2906096457572182E-4	0.0	0.0	5.588400352753886	0.0
12	2.2906096457572182E-4	0.0	0.0	6.522052844364528	0.0
13	2.2906096457572182E-4	0.0	0.0	6.873432364023685	0.0
14	2.2906096457572182E-4	0.0	0.0	7.019115137493844	0.0
15	2.2906096457572182E-4	0.0	0.0	7.283680551578803	0.0
16	2.2906096457572182E-4	0.0	0.0	7.870992864750954	0.0
17	2.2906096457572182E-4	0.0	0.0	8.638347096079622	0.0
18	2.2906096457572182E-4	0.0	0.0	9.489308579478427	0.0
19	2.2906096457572182E-4	0.0	0.0	9.974001580520655	0.0
20	4.5812192915144364E-4	0.0	0.0	10.460756130244064	0.0
21	4.5812192915144364E-4	0.0	0.0	11.023558920206613	0.0
22	4.5812192915144364E-4	0.0	0.0	11.658974035939666	0.0
23	4.5812192915144364E-4	0.0	0.0	12.266214653029904	0.0
24	4.5812192915144364E-4	0.0	0.0	12.745181129957738	0.0
25	4.5812192915144364E-4	0.0	0.0	13.14878654954016	0.0
26	4.5812192915144364E-4	0.0	0.0	13.558576615166126	0.0
27	4.5812192915144364E-4	0.0	0.0	13.97065729043785	0.0
28	4.5812192915144364E-4	0.0	0.0	14.38823542885939	0.0
29	4.5812192915144364E-4	0.0	0.0	14.835820553640351	0.0
30	4.5812192915144364E-4	0.0	0.0	15.359912040589602	0.0
31	4.5812192915144364E-4	0.0	0.0	15.83338105436762	0.0
32	4.5812192915144364E-4	0.0	0.0	16.2798208743257	0.0
33	4.5812192915144364E-4	0.0	0.0	16.72580257235463	0.0
34	4.5812192915144364E-4	0.0	0.0	17.186673233080985	0.0
35	4.5812192915144364E-4	0.0	0.0	17.669991868335757	0.0
36	4.5812192915144364E-4	0.0	0.0	18.163160125067286	0.0
37	4.5812192915144364E-4	0.0	0.0	18.617846139750096	0.0
38	4.5812192915144364E-4	0.0	0.0	19.082610836874235	0.0
39	4.5812192915144364E-4	0.0	0.0	19.546917412069224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	45	3.8380676E-10	45.000004	1
ATTACGG	35	1.2096098E-7	45.000004	2
TAAAGCG	20	7.028475E-4	45.0	1
CCGATAC	20	7.028475E-4	45.0	11
CGGCCAG	20	7.028475E-4	45.0	10
GATCCGC	20	7.028475E-4	45.0	9
TAGCACG	25	3.8868173E-5	45.0	1
TCGTCAA	20	7.028475E-4	45.0	33
CTCGATT	20	7.028475E-4	45.0	28
TTGCACG	20	7.028475E-4	45.0	1
AATACCG	20	7.028475E-4	45.0	16
TAGTACG	25	3.8868173E-5	45.0	1
GTCGGAG	40	6.7975634E-9	45.0	1
CGGACTA	20	7.028475E-4	45.0	6
GTAACGT	90	0.0	42.500004	41
AACGTTG	85	0.0	42.35294	43
ACGTTGC	85	0.0	42.35294	44
TAGACGG	50	1.0786607E-9	40.5	2
CGTTCGG	45	1.9235813E-8	40.000004	2
AACGCAT	40	3.4516597E-7	39.375	42
>>END_MODULE