##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397543
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.783243573651152	31.0	31.0	34.0	30.0	34.0
2	31.895963455525568	31.0	31.0	34.0	30.0	34.0
3	31.82870029154079	33.0	31.0	34.0	30.0	34.0
4	35.57536668989267	37.0	35.0	37.0	33.0	37.0
5	31.15653904105971	37.0	35.0	37.0	0.0	37.0
6	33.31653934291385	37.0	35.0	37.0	17.0	37.0
7	29.50770105372249	35.0	32.0	37.0	0.0	37.0
8	32.386101629257716	35.0	33.0	37.0	17.0	37.0
9	36.26756602430429	39.0	35.0	39.0	32.0	39.0
10	36.66207177588336	38.0	35.0	39.0	32.0	39.0
11	36.76572848723283	39.0	35.0	39.0	33.0	39.0
12	36.79625097159301	39.0	35.0	39.0	33.0	39.0
13	36.539197017681104	39.0	35.0	39.0	32.0	39.0
14	37.86401219490722	40.0	37.0	41.0	33.0	41.0
15	37.87781195996408	40.0	36.0	41.0	33.0	41.0
16	37.97144962934827	40.0	37.0	41.0	33.0	41.0
17	37.86837147176532	40.0	36.0	41.0	33.0	41.0
18	37.727800514661304	40.0	36.0	41.0	33.0	41.0
19	37.607599681040796	39.0	36.0	41.0	32.0	41.0
20	37.65335322216716	39.0	36.0	41.0	32.0	41.0
21	37.649102109708885	39.0	36.0	41.0	33.0	41.0
22	37.65768985996483	39.0	36.0	41.0	33.0	41.0
23	37.66878803047721	39.0	36.0	41.0	33.0	41.0
24	37.574775558870364	39.0	36.0	41.0	33.0	41.0
25	37.48742400193186	39.0	36.0	41.0	33.0	41.0
26	37.24867750155329	39.0	35.0	41.0	32.0	41.0
27	37.24635825558493	39.0	35.0	41.0	32.0	41.0
28	37.166832267201286	39.0	35.0	41.0	32.0	41.0
29	37.06227753978815	39.0	35.0	41.0	31.0	41.0
30	36.8909803467801	39.0	35.0	41.0	31.0	41.0
31	36.82275376500152	39.0	35.0	40.0	31.0	41.0
32	36.61378517544014	39.0	35.0	40.0	30.0	41.0
33	36.51149938497219	39.0	35.0	40.0	30.0	41.0
34	36.404230485758774	39.0	35.0	40.0	30.0	41.0
35	36.04426187858923	39.0	35.0	40.0	29.0	41.0
36	36.0721028920142	39.0	35.0	40.0	29.0	41.0
37	35.71164880277102	38.0	35.0	40.0	27.0	41.0
38	35.77286230671902	38.0	35.0	40.0	27.0	41.0
39	35.74015641075305	38.0	35.0	40.0	27.0	41.0
40	35.52533436634528	38.0	35.0	40.0	26.0	41.0
41	35.494067308442105	38.0	35.0	40.0	26.0	41.0
42	35.359800071941905	38.0	34.0	40.0	25.0	41.0
43	35.24103053003071	38.0	34.0	40.0	25.0	41.0
44	35.09651031460748	38.0	34.0	40.0	24.0	41.0
45	34.99221467866369	38.0	34.0	40.0	24.0	41.0
46	34.76048880246917	38.0	34.0	40.0	23.0	41.0
47	34.48593988574821	38.0	33.0	40.0	23.0	41.0
48	34.36227024497979	38.0	33.0	40.0	22.0	41.0
49	34.20672229167663	38.0	33.0	40.0	22.0	41.0
50	34.0913813096948	37.0	33.0	40.0	21.0	41.0
51	31.688255106994713	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	12.0
10	13.0
11	12.0
12	10.0
13	4.0
14	8.0
15	22.0
16	44.0
17	96.0
18	237.0
19	349.0
20	579.0
21	945.0
22	1551.0
23	2350.0
24	3225.0
25	4167.0
26	5060.0
27	5942.0
28	6939.0
29	8109.0
30	9791.0
31	12177.0
32	15485.0
33	21049.0
34	28392.0
35	34516.0
36	38486.0
37	57296.0
38	72038.0
39	68622.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46900838399871	20.027267490560767	25.2772656039724	11.226458521468118
2	28.275934930309425	31.402389175510574	24.2014071433782	16.1202687508018
3	28.72645223284022	31.199643812115923	24.98245472816777	15.091449226876087
4	25.76148995203035	23.475699484080966	33.167481253600236	17.595329310288445
5	25.465924440878098	36.31456219830307	21.026153145697446	17.193360215121384
6	24.418490578377686	38.34729827968295	24.53998686934495	12.694224272594411
7	67.30441738377986	18.33763894723338	10.495468414737525	3.862475254249226
8	78.94164907947065	8.769617374724243	8.298724917807633	3.9900086279974745
9	74.04959966594808	6.927552491177055	8.145785487356084	10.877062355518774
10	36.86242746067721	32.07653008605359	16.907604963488225	14.153437489780979
11	26.860239018169104	25.212366964076843	29.824949753863105	18.10244426389095
12	25.804504166844843	22.981161786272178	31.88133107613516	19.333002970747817
13	20.784669834458157	25.822866960303664	36.23457085145506	17.157892353783115
14	18.61811175143318	31.741975081940822	28.912344073471296	20.727569093154703
15	16.812772454803632	26.66981936545229	39.75695710904229	16.760451070701784
16	19.145853404537373	27.87723592164873	31.22354059812398	21.753370075689926
17	20.135683435502575	24.486407759663734	31.81165307903799	23.566255725795703
18	20.101976389975423	27.081347175022575	32.2043150049177	20.61236143008429
19	21.093315691635873	29.09622355317538	28.16299117328188	21.647469581906865
20	22.90343434546703	28.372025164573394	30.991867546403785	17.73267294355579
21	21.444472673396337	29.872240235647464	29.480584490231244	19.20270260072495
22	19.829553029483606	25.300659299748705	28.915362614861788	25.9544250559059
23	19.078690858598943	30.363256301834014	30.049327997222942	20.508724842344098
24	21.756388617080415	25.598237171827954	29.367389188087827	23.2779850230038
25	18.727785421954355	32.49057334678261	27.46721738277369	21.314423848489344
26	19.67158269671457	27.998732212615995	29.697416380114856	22.63226871055458
27	21.07294053725006	29.568625280787238	28.210784745297996	21.14764943666471
28	18.486553655830942	26.68742752356349	33.73874021175068	21.08727860885489
29	25.02496585275052	24.53520751214334	29.023527014687716	21.416299620418418
30	19.87835278196321	27.48558017623251	32.91417532191486	19.72189171988942
31	23.261131500240225	25.938074623374074	29.56661291986024	21.23418095652546
32	26.229363867556465	27.96477362197297	26.9286592896869	18.877203220783663
33	20.76228231914535	25.310721104383678	28.51037497830423	25.41662159816674
34	24.492696387560592	25.288585134186743	30.413062234777115	19.805656243475546
35	20.561800861793568	27.505452240386575	29.494922561836077	22.43782433598378
36	25.118037545623995	25.391215541463442	30.615052962824148	18.87569395008842
37	21.969950420457664	26.860490563284976	32.8316685239081	18.337890492349253
38	20.8468014780791	26.79936510012753	27.719014043763817	24.634819378029547
39	23.83339663885416	25.408572154458763	30.62561785769087	20.13241334899621
40	19.858732262925017	27.422190807032194	30.913133925135146	21.805943004907647
41	21.750854624531183	26.905014048794722	29.881798950050687	21.462332376623408
42	21.158465876647305	26.81269699126887	32.29562588198006	19.733211250103764
43	24.695190205839367	24.707767461633082	27.847553597975566	22.74948873455199
44	22.520079588874662	25.026223578329894	29.52762342689973	22.926073405895714
45	20.468226078688343	24.994025803497983	28.793614778778647	25.744133339035024
46	24.276619133024603	27.50394296969133	27.634746429945945	20.584691467338125
47	19.618003587033353	25.14771986929716	33.94475566165169	21.289520882017797
48	22.780680328920393	25.577862017442136	28.536535670355157	23.10492198328231
49	20.415150059238872	23.39948131397107	32.948385457673766	23.236983169116296
50	20.72782063827058	24.71657154068868	30.342126512100577	24.213481308940167
51	20.25290345949998	24.58778044136106	28.38736941664172	26.771946682497237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	450.5
2	678.0
3	1154.0
4	1630.0
5	1336.0
6	1042.0
7	918.5
8	795.0
9	815.0
10	835.0
11	886.5
12	938.0
13	941.5
14	945.0
15	977.5
16	1010.0
17	1076.0
18	1142.0
19	1155.0
20	1168.0
21	1421.0
22	1674.0
23	1840.5
24	2007.0
25	2730.0
26	4066.0
27	4679.0
28	5577.5
29	6476.0
30	7472.5
31	8469.0
32	10110.0
33	11751.0
34	13043.5
35	14336.0
36	15950.0
37	17564.0
38	18515.5
39	19467.0
40	20641.0
41	21815.0
42	24149.5
43	26484.0
44	31759.5
45	37035.0
46	45837.5
47	54640.0
48	45130.5
49	35621.0
50	33238.0
51	30855.0
52	26567.5
53	22280.0
54	19877.0
55	17474.0
56	15251.5
57	13029.0
58	12021.0
59	11013.0
60	9959.5
61	8906.0
62	7668.0
63	6430.0
64	5372.5
65	4315.0
66	3417.5
67	2520.0
68	2098.0
69	1676.0
70	1382.0
71	1088.0
72	925.0
73	762.0
74	632.5
75	405.5
76	308.0
77	257.5
78	207.0
79	164.5
80	122.0
81	104.0
82	86.0
83	65.5
84	45.0
85	33.5
86	22.0
87	16.5
88	11.0
89	10.0
90	9.0
91	7.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.565899148634735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74634338750907	26.96601354888243
2	8.983263783617282	6.5696223507466485
3	4.021498927216179	4.411491725967887
4	2.4801438948510692	3.6275476613290536
5	1.7640133279311785	3.22513667229895
6	1.2693690775438546	2.7849373003118454
7	0.9421498431926523	2.4115389304359183
8	0.7159273472752469	2.094282174257302
9	0.6088918324303773	2.0038209603368915
>10	5.365794497935037	36.99417753581045
>50	0.07161627095169631	1.626120265030172
>100	0.020658539697604707	1.243133438905847
>500	0.006886179899201568	1.9086391466287866
>1k	0.0027544719596806276	2.721195680424446
>5k	6.886179899201569E-4	1.4123426086333597
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	5609	1.410916554938711	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	4322	1.0871779908085415	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC	4190	1.0539740355131395	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1235	0.3106582181047082	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	1060	0.2666378228267131	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTC	928	0.23343386753131107	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTT	882	0.22186279220109523	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	846	0.21280716802962196	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	799	0.20098454758353182	No Hit
GAATATGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	795	0.1999783671200348	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	763	0.19192892341205858	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC	705	0.17733930669135162	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC	658	0.16551668624526153	No Hit
GAATCTATCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	626	0.15746724253728528	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.14539307697532092	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTA	415	0.10439122308781691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.515451158742576E-4	0.0	0.0	0.07772744080514561	0.0
2	2.515451158742576E-4	0.0	0.0	0.6323844213078836	0.0
3	2.515451158742576E-4	0.0	0.0	0.8069567317246185	0.0
4	2.515451158742576E-4	0.0	0.0	1.2911810797825645	0.0
5	2.515451158742576E-4	0.0	0.0	3.558860299388997	0.0
6	2.515451158742576E-4	0.0	0.0	4.042581557215194	0.0
7	2.515451158742576E-4	0.0	0.0	4.938836805075174	0.0
8	2.515451158742576E-4	0.0	0.0	6.0056396414979005	0.0
9	2.515451158742576E-4	0.0	0.0	6.296174250332668	0.0
10	2.515451158742576E-4	0.0	0.0	9.299622933871305	0.0
11	2.515451158742576E-4	0.0	0.0	10.276875709042795	0.0
12	2.515451158742576E-4	0.0	0.0	12.714096336748478	0.0
13	2.515451158742576E-4	0.0	0.0	13.120593244001277	0.0
14	2.515451158742576E-4	0.0	0.0	13.292147013027522	0.0
15	2.515451158742576E-4	0.0	0.0	13.692606837499339	0.0
16	2.515451158742576E-4	0.0	0.0	14.197709430174848	0.0
17	2.515451158742576E-4	0.0	0.0	14.794625990144462	0.0
18	2.515451158742576E-4	0.0	0.0	15.46625144952873	0.0
19	2.515451158742576E-4	0.0	0.0	15.876521533519645	0.0
20	2.515451158742576E-4	0.0	0.0	16.258115474300894	0.0
21	2.515451158742576E-4	0.0	0.0	16.72347393866827	0.0
22	2.515451158742576E-4	0.0	0.0	17.203422019756353	0.0
23	2.515451158742576E-4	0.0	0.0	17.715567875676342	0.0
24	2.515451158742576E-4	0.0	0.0	18.07578048160828	0.0
25	2.515451158742576E-4	0.0	0.0	18.39499123365271	0.0
26	2.515451158742576E-4	0.0	0.0	18.701624729903433	0.0
27	2.515451158742576E-4	0.0	0.0	19.030394196351086	0.0
28	2.515451158742576E-4	0.0	0.0	19.372244008824204	0.0
29	2.515451158742576E-4	0.0	0.0	19.719879358962427	0.0
30	2.515451158742576E-4	0.0	0.0	20.147757601064537	0.0
31	2.515451158742576E-4	0.0	0.0	20.54947515111573	0.0
32	2.515451158742576E-4	0.0	0.0	20.911448572858784	0.0
33	2.515451158742576E-4	0.0	0.0	21.26436637043037	0.0
34	2.515451158742576E-4	0.0	0.0	21.610995540105094	0.0
35	2.515451158742576E-4	0.0	0.0	22.00416055621656	0.0
36	2.515451158742576E-4	0.0	0.0	22.372171060740598	0.0
37	2.515451158742576E-4	0.0	0.0	22.74722482850912	0.0
38	2.515451158742576E-4	0.0	0.0	23.132340400912607	0.0
39	2.515451158742576E-4	0.0	0.0	23.499847815204895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	30	2.1619053E-6	45.000004	2
CGCGGGT	35	1.209246E-7	45.000004	4
TACGGGT	35	1.209246E-7	45.000004	4
ACGATGG	30	2.1619053E-6	45.000004	2
TAGGCGA	30	2.1619053E-6	45.000004	6
GGTAGCG	30	2.1619053E-6	45.000004	1
TCGCCGC	20	7.0277124E-4	45.0	28
CGGGTAC	25	3.886186E-5	45.0	6
ACTTGCG	20	7.0277124E-4	45.0	1
TAGCGCA	20	7.0277124E-4	45.0	19
CGCGAAG	20	7.0277124E-4	45.0	2
ACGGGTA	40	6.7957444E-9	45.0	5
TCTAGCG	20	7.0277124E-4	45.0	1
ATCGTTC	20	7.0277124E-4	45.0	30
GCCGCGG	20	7.0277124E-4	45.0	30
CACTACG	20	7.0277124E-4	45.0	16
ACGTGCG	25	3.886186E-5	45.0	1
AGACCGA	20	7.0277124E-4	45.0	7
AGCGCAT	20	7.0277124E-4	45.0	20
CGTCGGG	20	7.0277124E-4	45.0	2
>>END_MODULE