##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560519
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86773508123721	31.0	31.0	34.0	30.0	34.0
2	32.010492061821274	33.0	31.0	34.0	30.0	34.0
3	31.868434433087906	33.0	31.0	34.0	30.0	34.0
4	35.639935488359896	37.0	35.0	37.0	33.0	37.0
5	31.10137568931651	37.0	35.0	37.0	0.0	37.0
6	33.36184678842287	37.0	35.0	37.0	17.0	37.0
7	29.634883028050787	35.0	32.0	37.0	0.0	37.0
8	32.677759362305295	35.0	35.0	37.0	17.0	37.0
9	36.51942931461735	39.0	35.0	39.0	32.0	39.0
10	36.762077645896035	38.0	35.0	39.0	33.0	39.0
11	36.90763024982204	39.0	37.0	39.0	33.0	39.0
12	37.00622280422252	39.0	37.0	39.0	33.0	39.0
13	36.888922587815934	39.0	37.0	39.0	33.0	39.0
14	38.165013139608114	40.0	37.0	41.0	33.0	41.0
15	38.247734688743826	40.0	37.0	41.0	34.0	41.0
16	38.26542365200823	40.0	37.0	41.0	34.0	41.0
17	38.23237927706286	40.0	37.0	41.0	34.0	41.0
18	38.119251978969494	40.0	37.0	41.0	33.0	41.0
19	38.02670560676801	40.0	37.0	41.0	34.0	41.0
20	37.959271675001204	40.0	37.0	41.0	33.0	41.0
21	37.878867620901346	40.0	36.0	41.0	33.0	41.0
22	37.86675919995576	40.0	36.0	41.0	33.0	41.0
23	37.78163630492454	39.0	36.0	41.0	33.0	41.0
24	37.76175829900503	40.0	36.0	41.0	33.0	41.0
25	37.638413684460296	39.0	36.0	41.0	33.0	41.0
26	37.474023182086604	39.0	36.0	41.0	33.0	41.0
27	37.41033399403053	39.0	36.0	41.0	33.0	41.0
28	37.280561408266266	39.0	36.0	41.0	32.0	41.0
29	37.24145301051347	39.0	36.0	41.0	32.0	41.0
30	37.00513274304707	39.0	35.0	41.0	31.0	41.0
31	36.88866925117614	39.0	35.0	41.0	31.0	41.0
32	36.7230209859077	39.0	35.0	41.0	30.0	41.0
33	36.48500942876156	39.0	35.0	41.0	30.0	41.0
34	36.3570369603885	39.0	35.0	40.0	30.0	41.0
35	36.01796906081685	39.0	35.0	40.0	28.0	41.0
36	35.82897100722723	39.0	35.0	40.0	27.0	41.0
37	35.68457804284957	39.0	35.0	40.0	26.0	41.0
38	35.515623912837924	39.0	35.0	40.0	25.0	41.0
39	35.55409361680871	39.0	35.0	40.0	26.0	41.0
40	35.36893843027622	38.0	35.0	40.0	24.0	41.0
41	35.19086061311035	38.0	34.0	40.0	24.0	41.0
42	35.07857003955263	38.0	34.0	40.0	24.0	41.0
43	34.88225376838252	38.0	34.0	40.0	23.0	41.0
44	34.74999063368057	38.0	34.0	40.0	23.0	41.0
45	34.63092598109966	38.0	34.0	40.0	23.0	41.0
46	34.4531924876766	38.0	33.0	40.0	22.0	41.0
47	34.22375155882316	38.0	33.0	40.0	21.0	41.0
48	34.050854654347134	38.0	33.0	40.0	20.0	41.0
49	33.85138594766636	38.0	33.0	40.0	19.0	41.0
50	33.72763099912759	37.0	33.0	40.0	19.0	41.0
51	31.542258157172192	35.0	28.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	22.0
10	30.0
11	19.0
12	19.0
13	6.0
14	15.0
15	26.0
16	46.0
17	103.0
18	206.0
19	437.0
20	800.0
21	1417.0
22	2312.0
23	3389.0
24	4903.0
25	6354.0
26	7732.0
27	8824.0
28	9857.0
29	11267.0
30	13233.0
31	15955.0
32	20264.0
33	27140.0
34	38356.0
35	47283.0
36	55123.0
37	84030.0
38	103796.0
39	97527.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.948155191884666	20.856206480065794	26.580544102876086	12.615094225173456
2	32.139142473314905	25.66799698136905	25.780571220600905	16.41228932471513
3	31.023034009551864	25.01984767688517	27.52752359866481	16.42959471489816
4	27.489166290527173	26.192154057221966	27.865959940697817	18.45271971155304
5	21.91130006297735	39.30678531860651	22.035649103777036	16.74626551463911
6	24.7165573334713	34.88980748199437	26.324352965733546	14.06928221880079
7	69.53323616148606	18.98240737602115	7.6803819317454005	3.8039745307473964
8	83.50278937912898	4.731329357256399	8.073053723424184	3.6928275401904305
9	77.80449904463542	6.984776608821468	9.502086459156603	5.708637887386511
10	37.94572530101566	29.051111559108612	18.631304201998507	14.371858937877215
11	27.46311900221045	26.672780048490775	27.226195722178907	18.637905227119866
12	24.66624681768147	23.51891728915523	31.82621820134554	19.988617691817762
13	22.617074532709864	25.26801054023146	33.55693562573258	18.557979301326093
14	20.411261705669208	28.22687544936032	31.388231264239035	19.97363158073143
15	19.609683168634785	26.56680683438028	34.80452937366976	19.018980623315176
16	22.281314281942272	25.712420096374967	31.874387844123035	20.131877777559726
17	22.523411338420285	24.79737528968688	29.50872316549484	23.170490206397997
18	22.604586106804586	26.496336431057642	30.49637924851789	20.40269821361988
19	22.855960279669375	27.527345192580448	29.080013344775107	20.536681182975062
20	23.74335214328149	27.734117844354966	29.227019958288658	19.295510054074885
21	22.978525259625453	27.199791621693464	30.059997966170638	19.76168515251044
22	21.851177212547658	25.65497333721069	28.989561460004033	23.504287990237618
23	20.28994556830366	27.929829318899092	30.345090888979676	21.43513422381757
24	21.50988637316487	25.674776412574772	31.248004081931207	21.567333132329143
25	21.16449219384178	28.161935634652885	28.929973827827425	21.743598343677913
26	20.48726269760704	28.62775392091972	28.825249456307457	22.059733925165784
27	21.129524601307004	27.19533146958444	29.623973495992107	22.05117043311645
28	19.82591134288044	27.147161826806943	31.416062613399365	21.610864216913253
29	21.977845532444036	25.423223833625624	30.39326053175718	22.205670102173166
30	22.027442423896424	25.56095333075239	30.98485510749859	21.426749137852596
31	22.693789148985136	26.5832201941415	28.343374622448124	22.379616034425236
32	23.173166297663418	26.261910836207157	29.429689270122868	21.135233596006557
33	22.454011371603816	25.796984580362132	28.763164138949794	22.98583990908426
34	22.479701847751816	25.505112226347364	29.677852133469163	22.337333792431657
35	21.967319573466735	24.900672412531957	31.162904379690964	21.969103634310343
36	23.605801052239087	26.437819235387206	29.036125448022283	20.92025426435143
37	21.789270301274357	26.301516986935326	30.967906529484278	20.94130618230604
38	21.424965077008988	27.181951013257354	28.546757558619777	22.84632635111388
39	22.957651747755207	26.085288812689665	29.08697118206519	21.870088257489932
40	24.07144093242156	24.626283854784585	30.56435196665947	20.737923246134386
41	21.570366035763282	26.00286520171484	29.572592543696107	22.854176218825767
42	21.16181610257636	26.70917488970044	30.085688442318638	22.04332056540456
43	21.950370995452428	25.901530545797733	29.693195056724214	22.45490340202562
44	21.444946558457428	25.9869870602067	29.23576185642235	23.332304524913518
45	22.136270135356696	25.181126777147604	29.027561955972946	23.65504113152275
46	23.946199861200064	25.825172741691183	28.28610626936821	21.94252112774054
47	21.757157206089357	25.329203827167323	31.03427359286661	21.87936537387671
48	21.660461108365638	24.873376281624708	30.318508382409874	23.14765422759978
49	21.411763026766266	24.572583623391893	30.957558976591336	23.05809437325051
50	20.624456976480726	24.635917783340084	30.674250114625913	24.06537512555328
51	21.134698377753473	24.620039641831944	29.44075044735326	24.804511533061323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	323.0
1	572.5
2	822.0
3	2418.5
4	4015.0
5	3122.5
6	2230.0
7	1865.0
8	1500.0
9	1455.5
10	1411.0
11	1485.5
12	1560.0
13	1594.5
14	1629.0
15	1578.0
16	1527.0
17	1522.0
18	1517.0
19	1704.0
20	1891.0
21	2055.5
22	2220.0
23	2738.5
24	3257.0
25	3754.5
26	5181.0
27	6110.0
28	7011.5
29	7913.0
30	9141.0
31	10369.0
32	12305.0
33	14241.0
34	16597.5
35	18954.0
36	20366.0
37	21778.0
38	23690.5
39	25603.0
40	27956.5
41	30310.0
42	32973.5
43	35637.0
44	38842.5
45	42048.0
46	46694.5
47	51341.0
48	52395.0
49	53449.0
50	51144.5
51	48840.0
52	43719.0
53	38598.0
54	34634.0
55	30670.0
56	27293.5
57	23917.0
58	21716.0
59	19515.0
60	17861.5
61	16208.0
62	14229.0
63	12250.0
64	10234.5
65	8219.0
66	6712.0
67	5205.0
68	4490.5
69	3776.0
70	3129.5
71	2483.0
72	2186.5
73	1890.0
74	1589.0
75	1015.5
76	743.0
77	580.0
78	417.0
79	331.0
80	245.0
81	204.0
82	163.0
83	125.5
84	88.0
85	70.5
86	53.0
87	42.0
88	31.0
89	18.0
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.94581381801472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.82615240832324	32.99361637682273
2	8.459051386262836	7.265616918229262
3	3.4974326005052214	4.50600267907057
4	2.1546812471425136	3.7013815870780062
5	1.4663425901266136	3.148663793450151
6	1.0842672675557727	2.7938844120850663
7	0.8107930122815996	2.437411602527503
8	0.6246273066040771	2.1460102412053352
9	0.511072281021267	1.9753573525458694
>10	4.508070430732931	36.271011683217075
>50	0.04345159913955181	1.1708638661160862
>100	0.012353886025067139	0.9544369980536496
>500	0.001277988209489704	0.32619535509481645
>1k	4.259960698299014E-4	0.3095471345038864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1692	0.301863094738983	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCG	640	0.11417989399110466	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	609	0.10864930537591054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06369097211691307	0.0
2	0.0	0.0	0.0	0.4171134252362543	0.0
3	0.0	0.0	0.0	0.6385153759283807	0.0
4	0.0	0.0	0.0	0.9228946743999757	0.0
5	0.0	0.0	0.0	1.8745127283820888	0.0
6	0.0	0.0	0.0	2.5048214244298586	0.0
7	0.0	0.0	0.0	3.134238090055823	0.0
8	0.0	0.0	0.0	4.147406243142516	0.0
9	0.0	0.0	0.0	4.569158226572159	0.0
10	0.0	0.0	0.0	5.700966425758984	0.0
11	0.0	0.0	0.0	6.857751476756364	0.0
12	0.0	0.0	0.0	7.961728326782857	0.0
13	0.0	0.0	0.0	8.394541487442888	0.0
14	0.0	0.0	0.0	8.57491003873196	0.0
15	0.0	0.0	0.0	8.879270818652	0.0
16	0.0	0.0	0.0	9.49352296710727	0.0
17	0.0	0.0	0.0	10.249251140460894	0.0
18	0.0	0.0	0.0	11.057965920869766	0.0
19	0.0	0.0	0.0	11.528957983583073	0.0
20	0.0	0.0	0.0	11.970334636292437	0.0
21	0.0	0.0	0.0	12.538379608898182	0.0
22	0.0	0.0	0.0	13.173862081392423	0.0
23	0.0	0.0	0.0	13.807738899127417	0.0
24	0.0	0.0	0.0	14.282299083527944	0.0
25	0.0	0.0	0.0	14.73206082220228	0.0
26	0.0	0.0	0.0	15.134366542436563	0.0
27	0.0	0.0	0.0	15.558973023215984	0.0
28	1.7840608436110104E-4	0.0	0.0	15.98768284393571	0.0
29	1.7840608436110104E-4	0.0	0.0	16.44029907995982	0.0
30	1.7840608436110104E-4	0.0	0.0	16.962493688884766	0.0
31	1.7840608436110104E-4	0.0	0.0	17.437232279369656	0.0
32	1.7840608436110104E-4	0.0	0.0	17.879500962500824	0.0
33	1.7840608436110104E-4	0.0	0.0	18.342821563586604	0.0
34	1.7840608436110104E-4	0.0	0.0	18.78758793189883	0.0
35	1.7840608436110104E-4	0.0	0.0	19.250730126900248	0.0
36	1.7840608436110104E-4	0.0	0.0	19.714942758407833	0.0
37	1.7840608436110104E-4	0.0	0.0	20.16773740051631	0.0
38	1.7840608436110104E-4	0.0	0.0	20.626241037324338	0.0
39	1.7840608436110104E-4	0.0	0.0	21.06940175087731	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAAA	20	7.0301886E-4	45.000004	1
CGTGAAC	20	7.0301886E-4	45.000004	28
ATTCGGA	20	7.0301886E-4	45.000004	18
GTTCGCA	20	7.0301886E-4	45.000004	7
TGACACG	20	7.0301886E-4	45.000004	1
GTGCGTA	20	7.0301886E-4	45.000004	9
CCCGACA	20	7.0301886E-4	45.000004	17
CGTTATT	110	0.0	45.0	1
AACGGCT	25	3.888239E-5	45.0	7
ATGCGCG	25	3.888239E-5	45.0	1
TGTAACG	35	1.2104465E-7	45.0	1
AGTAACG	35	1.2104465E-7	45.0	1
AGGCGAT	130	0.0	43.26923	7
TAGGCGA	60	3.6379788E-12	41.249996	6
CGTTTTT	695	0.0	40.143887	1
CGGTTTT	230	0.0	40.1087	1
CGATGAT	45	1.9252184E-8	40.0	10
TTTGACG	45	1.9252184E-8	40.0	1
TAGCCGT	40	3.453988E-7	39.375004	44
GTAACGG	105	0.0	38.57143	2
>>END_MODULE