##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936047.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	823590
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95706480166102	33.0	31.0	34.0	30.0	34.0
2	32.08646899549533	33.0	31.0	34.0	30.0	34.0
3	31.891273570587305	33.0	31.0	34.0	30.0	34.0
4	35.68288225937663	37.0	35.0	37.0	33.0	37.0
5	31.053179373231828	37.0	35.0	37.0	0.0	37.0
6	33.36486237083986	37.0	35.0	37.0	17.0	37.0
7	29.73410313384087	35.0	32.0	37.0	0.0	37.0
8	32.750549423863816	35.0	35.0	37.0	17.0	37.0
9	36.60221105161549	39.0	35.0	39.0	32.0	39.0
10	36.92669896429049	38.0	37.0	39.0	34.0	39.0
11	37.06302529171068	39.0	37.0	39.0	34.0	39.0
12	37.08863390764822	39.0	37.0	39.0	34.0	39.0
13	36.97105234400612	39.0	37.0	39.0	33.0	39.0
14	38.26861909445233	40.0	38.0	41.0	34.0	41.0
15	38.34125475054335	40.0	38.0	41.0	34.0	41.0
16	38.34781505360677	40.0	38.0	41.0	34.0	41.0
17	38.274669435034426	40.0	37.0	41.0	34.0	41.0
18	38.1885440571158	40.0	37.0	41.0	34.0	41.0
19	38.06277273886278	40.0	37.0	41.0	34.0	41.0
20	37.97480785342221	40.0	36.0	41.0	33.0	41.0
21	37.8561335130344	40.0	36.0	41.0	33.0	41.0
22	37.839988343714715	40.0	36.0	41.0	33.0	41.0
23	37.7585655483918	40.0	36.0	41.0	33.0	41.0
24	37.685276654646124	40.0	36.0	41.0	33.0	41.0
25	37.55598416687915	39.0	36.0	41.0	33.0	41.0
26	37.404633373401815	39.0	35.0	41.0	33.0	41.0
27	37.36104008062264	39.0	35.0	41.0	33.0	41.0
28	37.22779174103619	39.0	35.0	41.0	32.0	41.0
29	37.178298668026564	39.0	35.0	41.0	32.0	41.0
30	37.010064473827995	39.0	35.0	41.0	31.0	41.0
31	36.88177491227431	39.0	35.0	41.0	31.0	41.0
32	36.71901431537537	39.0	35.0	41.0	31.0	41.0
33	36.50188079019901	39.0	35.0	41.0	30.0	41.0
34	36.27672749790551	39.0	35.0	41.0	30.0	41.0
35	36.11223060017727	39.0	35.0	41.0	29.0	41.0
36	35.94018990031448	39.0	35.0	40.0	28.0	41.0
37	35.818634271907136	39.0	35.0	40.0	27.0	41.0
38	35.64385677339453	39.0	35.0	40.0	27.0	41.0
39	35.58734928787382	39.0	35.0	40.0	26.0	41.0
40	35.419605629014434	38.0	35.0	40.0	25.0	41.0
41	35.27865321337073	38.0	34.0	40.0	25.0	41.0
42	35.19591058657827	38.0	34.0	40.0	24.0	41.0
43	34.99083281729987	38.0	34.0	40.0	23.0	41.0
44	34.8167559101009	38.0	34.0	40.0	23.0	41.0
45	34.65294867591884	38.0	34.0	40.0	23.0	41.0
46	34.50821039594944	38.0	34.0	40.0	23.0	41.0
47	34.34079942689931	38.0	33.0	40.0	22.0	41.0
48	34.25046078752777	37.0	33.0	40.0	22.0	41.0
49	34.07512475867847	37.0	33.0	40.0	21.0	41.0
50	33.89726077295742	37.0	33.0	40.0	20.0	41.0
51	31.944535509173253	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	53.0
10	57.0
11	46.0
12	21.0
13	15.0
14	32.0
15	60.0
16	111.0
17	200.0
18	384.0
19	738.0
20	1281.0
21	2100.0
22	3291.0
23	4733.0
24	6763.0
25	9178.0
26	11384.0
27	13009.0
28	14354.0
29	15682.0
30	18334.0
31	22306.0
32	28493.0
33	39493.0
34	55995.0
35	68139.0
36	80249.0
37	125185.0
38	155358.0
39	146512.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8671911995046	20.098835585667626	24.95999222914314	13.073980985684624
2	32.77261744314525	23.652424143080903	25.665440328318702	17.909518085455144
3	32.55964739737005	23.777486370645587	27.57719253512063	16.08567369686373
4	26.997413761701814	26.046940832210204	28.0293592685681	18.92628613751988
5	21.253779186245584	39.6086645053971	22.920870821646695	16.21668548671062
6	25.21242365740235	34.98306196044148	25.54863463616605	14.255879745990116
7	70.20762758168506	18.70615233307835	7.6665573889920955	3.419662696244491
8	84.44128753384572	4.395269490887456	8.192911521509489	2.970531453757331
9	78.45347806554233	7.3906919705193115	9.663424762321057	4.492405201617309
10	38.62686530919511	32.07166187059095	16.34927573185687	12.952197088357071
11	29.531320195728455	25.454534416396505	27.185857040517735	17.8282883473573
12	26.226763316698843	23.76425163005865	30.295778239172407	19.71320681407011
13	23.653638339465026	24.776769994778956	31.33330904940565	20.23628261635037
14	20.81084034531745	27.105234400611955	30.693063296057506	21.390861958013087
15	20.615961825665682	25.580689420706904	33.48292232785731	20.320426425770105
16	24.7150888184655	25.917507497662672	30.52416857902597	18.843235104845856
17	23.15824621474277	25.48913901334402	29.64581891475127	21.706795857161936
18	24.922837819788974	25.24411418302796	29.30547966828155	20.527568328901516
19	24.372806857781175	27.09297101713231	27.722531842300175	20.811690282786337
20	26.506149904685582	26.228948870190266	27.984798261270775	19.280102963853373
21	25.113345232457895	27.00397042217608	28.81567284692626	19.067011498439758
22	24.816838475455018	24.12547505433529	29.407957843101546	21.649728627108146
23	23.57216576209036	26.73271894996297	29.049527070508386	20.645588217438288
24	23.22575553370002	24.879491008875775	30.553187872606514	21.341565584817687
25	23.244818416930755	26.0084508068335	28.597724595976153	22.149006180259594
26	22.518000461394628	26.988792967374543	28.557413276023265	21.935793295207567
27	22.358090797605605	26.345147464150852	28.94243494942872	22.354326788814824
28	21.03716655131801	27.29780594713389	29.97644459014801	21.68858291140009
29	22.833327262351414	26.16581065821586	29.569324542551513	21.431537536881216
30	22.80600784370864	25.560412341092047	30.20022098374191	21.4333588314574
31	24.322417707840067	25.8971089984094	27.86119306936704	21.919280224383492
32	24.37134982212023	26.425041586226154	27.709782780266877	21.49382581138673
33	23.109071261185786	26.29415121601768	27.644580434439465	22.95219708835707
34	21.786325720322004	27.442295316844543	29.4105076555082	21.360871307325247
35	21.7458929807307	26.627205284182665	28.678104396605107	22.948797338481526
36	21.89244648429437	29.552204373535375	26.95358127223497	21.601767869935284
37	22.30685171019561	28.373462523828604	26.91788389854175	22.40180186743404
38	22.2289003023349	28.986146019257152	26.68888646049612	22.096067217911823
39	22.775652934105562	27.19083524569264	26.41581369370682	23.61769812649498
40	23.358467198484682	26.17321725615901	28.206267681734843	22.26204786362146
41	20.82286088952027	27.1973919061669	28.049757767821365	23.929989436491457
42	22.859796743525298	27.19107808496946	27.52996029577824	22.419164875727
43	22.33380686992314	26.27812382374725	27.846015614565502	23.542053691764107
44	22.282082103959493	26.871015918114594	27.0849573209971	23.76194465692881
45	22.651197804732938	26.343569008851492	27.321118517709053	23.684114668706517
46	22.2525771318253	26.701878361806237	27.16133027355844	23.884214232810013
47	21.069464175135685	27.187556915455506	28.73432168919001	23.008657220218797
48	21.949756553624983	26.589322356998018	28.4759406986486	22.984980390728396
49	22.447212812200245	26.236841146687066	28.152843040833424	23.163103000279264
50	20.583664201848006	26.503600092278923	29.0151653128377	23.89757039303537
51	20.56751538993917	25.914472006702365	28.16037105841499	25.357641544943476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	1010.5
2	1533.0
3	5779.0
4	10025.0
5	7606.0
6	5187.0
7	4174.0
8	3161.0
9	3005.0
10	2849.0
11	2766.5
12	2684.0
13	2688.0
14	2692.0
15	2682.5
16	2673.0
17	2708.5
18	2744.0
19	2931.0
20	3118.0
21	3234.5
22	3351.0
23	3731.5
24	4112.0
25	4894.0
26	7040.0
27	8404.0
28	9551.0
29	10698.0
30	12852.0
31	15006.0
32	17502.5
33	19999.0
34	23399.5
35	26800.0
36	28052.0
37	29304.0
38	31424.0
39	33544.0
40	36468.0
41	39392.0
42	42367.5
43	45343.0
44	48896.5
45	52450.0
46	55294.5
47	58139.0
48	60202.5
49	62266.0
50	61807.0
51	61348.0
52	58804.5
53	56261.0
54	52595.5
55	48930.0
56	47032.0
57	45134.0
58	42208.5
59	39283.0
60	37052.0
61	34821.0
62	31122.5
63	27424.0
64	23177.0
65	18930.0
66	15769.5
67	12609.0
68	10465.0
69	8321.0
70	7224.0
71	6127.0
72	5286.0
73	4445.0
74	3840.0
75	2646.5
76	2058.0
77	1660.0
78	1262.0
79	1029.5
80	797.0
81	593.5
82	390.0
83	325.0
84	260.0
85	200.5
86	141.0
87	113.5
88	86.0
89	65.5
90	45.0
91	36.5
92	28.0
93	18.5
94	9.0
95	6.5
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	823590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.41266307948419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78600342966479	26.86744882866049
2	8.03588042825406	5.852156131620725
3	3.8837573531485217	4.2425384394799925
4	2.4854400348393053	3.6200596237146025
5	1.7961331326957855	3.2700995303375056
6	1.3031921240940334	2.8471617445484037
7	1.0087269935200478	2.571130530795857
8	0.8512671547632991	2.4797523277621707
9	0.6850202762524447	2.2449071269615906
>10	6.086668161524169	42.498259086631776
>50	0.0577497619426367	1.3611109277106637
>100	0.018110862931273074	1.1088961984822887
>500	0.0013668575797182421	0.34665448933695975
>1k	6.834287898591211E-4	0.6898250139569652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4285	0.5202831505967775	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.15286732476110684	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.10757779963331268	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04577520368144344	0.0
2	0.0	0.0	0.0	0.18747192170861715	0.0
3	0.0	0.0	0.0	0.27707961485690696	0.0
4	0.0	0.0	0.0	0.4004419674838208	0.0
5	0.0	0.0	0.0	0.7526803385179519	0.0
6	0.0	0.0	0.0	1.086584344151823	0.0
7	0.0	0.0	0.0	1.386976529583895	0.0
8	0.0	0.0	0.0	1.992981944899768	0.0
9	0.0	0.0	0.0	2.295802523100086	0.0
10	0.0	0.0	0.0	2.8708459306208187	0.0
11	0.0	0.0	0.0	3.4292548476790636	0.0
12	0.0	0.0	0.0	3.930717954321932	0.0
13	0.0	0.0	0.0	4.1578940977913765	0.0
14	0.0	0.0	0.0	4.259158076227249	0.0
15	1.2141963841231681E-4	0.0	0.0	4.413968115202953	0.0
16	1.2141963841231681E-4	0.0	0.0	4.685341007054481	0.0
17	1.2141963841231681E-4	0.0	0.0	5.041464806517806	0.0
18	1.2141963841231681E-4	0.0	0.0	5.443728068577812	0.0
19	1.2141963841231681E-4	0.0	0.0	5.689481416724341	0.0
20	1.2141963841231681E-4	0.0	0.0	5.956361781954613	0.0
21	1.2141963841231681E-4	0.0	0.0	6.253597056787965	0.0
22	1.2141963841231681E-4	0.0	0.0	6.5782731699025	0.0
23	1.2141963841231681E-4	0.0	0.0	6.91630544324239	0.0
24	1.2141963841231681E-4	0.0	0.0	7.170679585716194	0.0
25	1.2141963841231681E-4	0.0	0.0	7.407205041343387	0.0
26	1.2141963841231681E-4	0.0	0.0	7.634016925897595	0.0
27	1.2141963841231681E-4	0.0	0.0	7.869813863694314	0.0
28	1.2141963841231681E-4	0.0	0.0	8.119331220631626	0.0
29	1.2141963841231681E-4	0.0	0.0	8.385118809116186	0.0
30	1.2141963841231681E-4	0.0	0.0	8.686482351655556	0.0
31	1.2141963841231681E-4	0.0	0.0	8.96963294843308	0.0
32	1.2141963841231681E-4	0.0	0.0	9.25533335761726	0.0
33	1.2141963841231681E-4	0.0	0.0	9.548440364744593	0.0
34	1.2141963841231681E-4	0.0	0.0	9.82284874755643	0.0
35	1.2141963841231681E-4	0.0	0.0	10.117655629621535	0.0
36	1.2141963841231681E-4	0.0	0.0	10.414769484816475	0.0
37	1.2141963841231681E-4	0.0	0.0	10.73422455347928	0.0
38	1.2141963841231681E-4	0.0	0.0	11.056350854187157	0.0
39	2.4283927682463362E-4	0.0	0.0	11.398875654148302	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCCGT	20	7.032121E-4	45.0	37
GCGATCG	25	3.8898394E-5	45.0	9
CGTTTTA	165	0.0	42.272728	1
CGAATAT	120	0.0	41.249996	14
TTTCGAC	55	6.002665E-11	40.909092	24
CGTTATT	355	0.0	40.56338	1
GTTAACG	50	1.0804797E-9	40.5	1
CGTTTTT	1765	0.0	39.900852	1
TTTCGTC	130	0.0	39.807693	16
CGCATAG	40	3.4566256E-7	39.375	2
ATGACGG	120	0.0	39.374996	2
ACACGAC	190	0.0	37.894737	26
CACGACC	190	0.0	37.894737	27
CGACGTT	60	1.546141E-10	37.499996	27
TAGCACG	30	1.13964095E-4	37.499996	1
TAATTCG	30	1.13964095E-4	37.499996	36
AATAGCG	30	1.13964095E-4	37.499996	1
TCAAGCG	205	0.0	37.317074	17
GAACGGG	310	0.0	37.01613	3
ATTACGG	55	2.746674E-9	36.81818	2
>>END_MODULE