##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537906
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.016209151784885	33.0	31.0	34.0	30.0	34.0
2	32.14500674839098	33.0	31.0	34.0	30.0	34.0
3	32.04787453569955	33.0	31.0	34.0	30.0	34.0
4	35.72328064754809	37.0	35.0	37.0	33.0	37.0
5	32.10304774440144	37.0	35.0	37.0	0.0	37.0
6	33.86545418716281	37.0	35.0	37.0	17.0	37.0
7	35.499591006607105	37.0	35.0	37.0	32.0	37.0
8	35.728536212646816	37.0	35.0	37.0	35.0	37.0
9	37.67351172881507	39.0	37.0	39.0	35.0	39.0
10	37.32612575431395	39.0	37.0	39.0	34.0	39.0
11	37.2743081504947	39.0	37.0	39.0	34.0	39.0
12	37.205009797250824	39.0	37.0	39.0	34.0	39.0
13	37.12557584410659	39.0	37.0	39.0	33.0	39.0
14	38.32686008336029	40.0	38.0	41.0	34.0	41.0
15	38.48470922428826	40.0	38.0	41.0	34.0	41.0
16	38.41842069060394	40.0	38.0	41.0	34.0	41.0
17	38.43027592181534	40.0	38.0	41.0	34.0	41.0
18	38.31187047551059	40.0	37.0	41.0	34.0	41.0
19	38.14731570199998	40.0	37.0	41.0	34.0	41.0
20	38.16838443891684	40.0	36.0	41.0	34.0	41.0
21	38.09955828713567	40.0	36.0	41.0	34.0	41.0
22	38.16759619710507	40.0	36.0	41.0	34.0	41.0
23	38.15907240298491	40.0	36.0	41.0	34.0	41.0
24	38.12001353396318	40.0	36.0	41.0	34.0	41.0
25	37.8598119374017	40.0	35.0	41.0	34.0	41.0
26	37.892388261146	40.0	36.0	41.0	34.0	41.0
27	37.84413819514934	40.0	35.0	41.0	34.0	41.0
28	37.77470227140058	40.0	36.0	41.0	33.0	41.0
29	37.74732016374608	40.0	36.0	41.0	33.0	41.0
30	37.566374050484654	40.0	35.0	41.0	33.0	41.0
31	37.51511044680669	40.0	35.0	41.0	33.0	41.0
32	37.41402587069116	40.0	35.0	41.0	33.0	41.0
33	37.25224295694787	40.0	35.0	41.0	33.0	41.0
34	37.135285719066154	40.0	35.0	41.0	32.0	41.0
35	37.05441842998591	40.0	35.0	41.0	32.0	41.0
36	36.899744565035526	40.0	35.0	41.0	31.0	41.0
37	36.864162139853434	40.0	35.0	41.0	31.0	41.0
38	36.751813513885324	40.0	35.0	41.0	31.0	41.0
39	36.71009804687064	40.0	35.0	41.0	31.0	41.0
40	36.63425579934041	39.0	35.0	41.0	31.0	41.0
41	36.503530356605054	39.0	35.0	41.0	31.0	41.0
42	36.479858934460665	39.0	35.0	41.0	31.0	41.0
43	36.38895085758478	39.0	35.0	41.0	30.0	41.0
44	36.3199257862898	39.0	35.0	41.0	30.0	41.0
45	36.30998538778151	39.0	35.0	41.0	30.0	41.0
46	36.18246124787602	39.0	35.0	41.0	30.0	41.0
47	36.09968098515354	39.0	35.0	41.0	30.0	41.0
48	36.057857692608	39.0	35.0	41.0	30.0	41.0
49	36.02453960357386	39.0	35.0	41.0	29.0	41.0
50	35.90732209716939	39.0	35.0	41.0	29.0	41.0
51	34.949037564184074	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	20.0
10	25.0
11	11.0
12	8.0
13	17.0
14	14.0
15	17.0
16	30.0
17	73.0
18	140.0
19	274.0
20	479.0
21	839.0
22	1269.0
23	1858.0
24	2708.0
25	4002.0
26	5567.0
27	6389.0
28	6743.0
29	7027.0
30	7911.0
31	9990.0
32	12890.0
33	18810.0
34	32564.0
35	44709.0
36	40019.0
37	60264.0
38	100124.0
39	172984.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.762906530137236	21.143657070194422	24.749491546850194	15.34394485281815
2	34.635047759273924	24.96235401724465	23.941171877614302	16.46142634586712
3	31.178309964938112	24.089710841671224	28.63269790632564	16.099281287065025
4	28.457202559555018	24.88334392998033	28.93405167445613	17.725401836008523
5	22.625142682922295	35.93936487044205	24.40742434551762	17.028068101118038
6	24.593888151461407	33.260829959137844	27.508709700207845	14.636572189192906
7	75.19789703033616	6.322666042022212	13.392302744345667	5.087134183295966
8	75.27225946540845	6.486263399181269	12.632690470082133	5.608786665328143
9	68.83377393076114	8.469695448647162	15.006153491502234	7.69037712908947
10	38.391466166951105	25.055492967172704	22.51136815726168	14.041672708614517
11	29.528393436771484	24.654493536045333	27.74871445940369	18.0683985677795
12	27.11235792127249	21.657687402631687	31.549006703773525	19.6809479723223
13	23.504292571564548	24.228210876993376	33.58653742475451	18.68095912668756
14	19.143121660661897	27.668774841700966	32.51813513885325	20.669968358783876
15	18.512528211248807	25.11963056742256	37.29183165831949	19.07600956300915
16	20.31953538350567	24.94748153023019	34.6131108409276	20.119872245336545
17	19.53426806914219	25.07668626116831	31.6090543700944	23.779991299595096
18	20.283655508583283	25.454633337423267	33.35025078731228	20.91146036668117
19	22.176551293348652	26.790554483497115	29.912661320007583	21.120232903146647
20	23.349060988351127	26.719910170178434	30.745334686729652	19.18569415474079
21	22.466007071867576	27.178540488486835	30.893501838611208	19.46195060103438
22	20.66885292225779	25.760634757745777	29.964343212382833	23.6061691076136
23	20.314330013050608	26.769547095589193	31.16046298052076	21.75565991083944
24	21.78298810572851	24.643153264696807	31.777299379445477	21.796559250129206
25	20.468446159737947	26.37858659319658	29.892025744275024	23.26094150279045
26	20.021342018865752	27.7717668142761	30.905028016047414	21.30186315081074
27	21.2674705245898	26.48771346666518	31.898138336437963	20.346677672307056
28	18.935278654634825	26.20011674902306	33.11377824378237	21.75082635255974
29	21.34443564488963	24.66936602305979	32.2338847307894	21.752313601261186
30	21.72386996984603	24.841515060252163	32.741036537982474	20.69357843191933
31	22.637784296884586	25.590158875342535	30.295813766717604	21.47624306105528
32	22.68890847099679	25.518770937673125	29.338211509074075	22.454109082256007
33	21.4470558052894	25.26203463058601	31.554212074228584	21.736697489896002
34	22.04734656241053	24.244756518796965	31.10097303246292	22.606923886329582
35	21.699888084535214	22.899168256163716	30.86003874282867	24.540904916472396
36	21.919071361910817	24.61526735154469	33.030678222589074	20.43498306395541
37	20.789505973162598	25.24920711053604	32.73229895186148	21.22898796443988
38	19.467713689752483	25.448870248705163	30.833825984465683	24.249590077076665
39	22.162236524597233	23.44034087740237	31.720969834878215	22.67645276312218
40	23.672537580915627	22.336058716578734	32.084602142381755	21.90680156012389
41	20.97634159128175	24.128937026171858	30.881975661174998	24.01274572137139
42	21.177491978152315	24.125776622681286	31.894792026859715	22.801939372306684
43	22.101259327837948	23.117607909188596	31.70237922611014	23.078753536863317
44	20.607132101147783	24.958449989403352	30.781214561652035	23.65320334779683
45	21.610281350273098	23.855097359018117	29.42837596159924	25.106245329109544
46	22.860871602101483	24.79429491398125	29.61260889449086	22.732224589426405
47	20.599695857640555	24.410027030745148	32.32572233810369	22.664554773510616
48	20.142924600208957	23.462835514011743	32.10114778418534	24.293092101593956
49	20.514550869482772	21.796745156216886	34.04107780913394	23.647626165166404
50	19.801043304964065	22.54724803218406	32.66964860031307	24.982060062538807
51	19.545422434403037	22.677010481385224	30.0699378701855	27.70762921402624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	464.0
1	816.0
2	1168.0
3	5463.0
4	9758.0
5	6223.0
6	2688.0
7	2536.5
8	2385.0
9	2386.0
10	2387.0
11	2415.5
12	2444.0
13	2430.0
14	2416.0
15	2273.0
16	2130.0
17	2191.5
18	2253.0
19	2173.5
20	2094.0
21	2712.0
22	3330.0
23	3572.0
24	3814.0
25	4090.5
26	4742.0
27	5117.0
28	6000.0
29	6883.0
30	8241.5
31	9600.0
32	10303.0
33	11006.0
34	12535.5
35	14065.0
36	14860.0
37	15655.0
38	16974.5
39	18294.0
40	20667.0
41	23040.0
42	26997.0
43	30954.0
44	34811.5
45	38669.0
46	44857.5
47	51046.0
48	52922.0
49	54798.0
50	52790.5
51	50783.0
52	43498.0
53	36213.0
54	32153.5
55	28094.0
56	24891.5
57	21689.0
58	21163.0
59	20637.0
60	19103.0
61	17569.0
62	16326.0
63	15083.0
64	12240.0
65	9397.0
66	7625.0
67	5853.0
68	4818.5
69	3784.0
70	3423.5
71	3063.0
72	2628.5
73	2194.0
74	1693.0
75	911.5
76	631.0
77	578.5
78	526.0
79	370.0
80	214.0
81	148.0
82	82.0
83	72.0
84	62.0
85	34.5
86	7.0
87	4.5
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	537906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.929162902659435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.66827977038521	23.92905622415046
2	11.034186669963306	7.266930607071499
3	4.149609930219833	4.099295441241064
4	2.2440582896671644	2.9557984393405348
5	1.295463577717018	2.132926559255285
6	0.9166710390309094	1.8111125983439342
7	0.6644288640555696	1.5315360411200367
8	0.5683969436821811	1.4973468441507427
9	0.46724607466966706	1.3847419897583078
>10	5.686961794520928	42.27622651278778
>50	0.244559471202369	5.0152005485367965
>100	0.05383744799302727	3.0375609218837814
>500	0.0028636940421823015	0.7232736952330996
>1k	0.0028636940421823015	1.2505939695151715
>5k	5.727388084364603E-4	1.0883996076115299
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5771	1.072864032005592	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1705	0.31696987949567396	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1614	0.30005242551672595	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	1131	0.21025978516692506	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	1091	0.2028235416596952	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	1090	0.20263763557201447	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC	939	0.17456581633222162	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	835	0.1552315832134239	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	785	0.14593627882938653	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	712	0.132365134428692	No Hit
ACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	564	0.10485103345194142	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.859060876807472E-4	0.0	0.0	0.21211884604373255	0.0
2	1.859060876807472E-4	0.0	0.0	1.125103642643882	0.0
3	1.859060876807472E-4	0.0	0.0	1.5571493904139384	0.0
4	1.859060876807472E-4	0.0	0.0	2.1167267143329878	0.0
5	1.859060876807472E-4	0.0	0.0	3.648221064646983	0.0
6	1.859060876807472E-4	0.0	0.0	4.64523541287883	0.0
7	1.859060876807472E-4	0.0	0.0	5.697463869151859	0.0
8	3.718121753614944E-4	0.0	0.0	7.161102497462382	0.0
9	3.718121753614944E-4	0.0	0.0	7.7323919049053185	0.0
10	3.718121753614944E-4	0.0	0.0	9.074633857960313	0.0
11	3.718121753614944E-4	0.0	0.0	10.801329600339093	0.0
12	3.718121753614944E-4	0.0	0.0	12.233364193743888	0.0
13	3.718121753614944E-4	0.0	0.0	12.761895201020252	0.0
14	3.718121753614944E-4	0.0	0.0	12.98014894795745	0.0
15	3.718121753614944E-4	0.0	0.0	13.38337925213699	0.0
16	3.718121753614944E-4	0.0	0.0	14.175896903920016	0.0
17	3.718121753614944E-4	0.0	0.0	15.078099147434681	0.0
18	3.718121753614944E-4	0.0	0.0	16.063959130405685	0.0
19	3.718121753614944E-4	0.0	0.0	16.67075660059564	0.0
20	3.718121753614944E-4	0.0	0.0	17.243161444564663	0.0
21	3.718121753614944E-4	0.0	0.0	17.918372355021138	0.0
22	3.718121753614944E-4	0.0	0.0	18.639687975222436	0.0
23	3.718121753614944E-4	0.0	0.0	19.348361981461444	0.0
24	3.718121753614944E-4	0.0	0.0	19.901618498399348	0.0
25	3.718121753614944E-4	0.0	0.0	20.395385067279413	0.0
26	3.718121753614944E-4	0.0	0.0	20.865541563024024	0.0
27	5.577182630422415E-4	0.0	0.0	21.298702747320164	0.0
28	5.577182630422415E-4	0.0	0.0	21.782616293553147	0.0
29	5.577182630422415E-4	0.0	0.0	22.263369436295562	0.0
30	5.577182630422415E-4	0.0	0.0	22.781861514837164	0.0
31	7.436243507229888E-4	0.0	0.0	23.260011972352046	0.0
32	7.436243507229888E-4	0.0	0.0	23.72849531330753	0.0
33	7.436243507229888E-4	0.0	0.0	24.186567913352892	0.0
34	7.436243507229888E-4	0.0	0.0	24.633114335962045	0.0
35	0.001115436526084483	0.0	0.0	25.1540231936435	0.0
36	0.001115436526084483	0.0	0.0	25.619160225020728	0.0
37	0.001115436526084483	0.0	0.0	26.12686975047685	0.0
38	0.001115436526084483	0.0	0.0	26.599257119273627	0.0
39	0.001115436526084483	0.0	0.0	27.056400188880584	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	20	7.0299354E-4	45.000004	15
CGAACTA	20	7.0299354E-4	45.000004	12
ACGCATG	30	2.1633405E-6	45.000004	1
CGACGAT	20	7.0299354E-4	45.000004	20
GCGCAAC	20	7.0299354E-4	45.000004	15
CTAACGG	20	7.0299354E-4	45.000004	2
CGTAGTC	20	7.0299354E-4	45.000004	12
AACGCGC	20	7.0299354E-4	45.000004	31
GCGATAT	40	6.8030204E-9	45.000004	9
TCGTAGT	20	7.0299354E-4	45.000004	11
TCGTAAG	35	1.2103192E-7	45.000004	1
GTATACG	20	7.0299354E-4	45.000004	1
CCGAACT	30	2.1633405E-6	45.000004	11
GACGATT	20	7.0299354E-4	45.000004	42
TAGGACG	40	6.8030204E-9	45.000004	1
TAGTGCG	20	7.0299354E-4	45.000004	1
CGCCGAC	20	7.0299354E-4	45.000004	13
TGTCGTC	20	7.0299354E-4	45.000004	35
ATCGCAT	20	7.0299354E-4	45.000004	14
TCGCGCC	20	7.0299354E-4	45.000004	38
>>END_MODULE