##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936044.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	735311
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09544940848158	33.0	31.0	34.0	30.0	34.0
2	32.199874610878936	33.0	31.0	34.0	30.0	34.0
3	32.12641045761589	33.0	31.0	34.0	30.0	34.0
4	35.77034207294601	37.0	35.0	37.0	35.0	37.0
5	31.92893619162504	37.0	35.0	37.0	0.0	37.0
6	33.792485084542456	37.0	35.0	37.0	17.0	37.0
7	35.4771654442814	37.0	35.0	37.0	32.0	37.0
8	35.7206012149961	37.0	35.0	37.0	35.0	37.0
9	37.67445611448761	39.0	37.0	39.0	35.0	39.0
10	37.343514512906786	39.0	37.0	39.0	34.0	39.0
11	37.33230429029349	39.0	37.0	39.0	34.0	39.0
12	37.2814210585725	39.0	37.0	39.0	34.0	39.0
13	37.20393683761021	39.0	37.0	39.0	34.0	39.0
14	38.45263160757829	40.0	38.0	41.0	34.0	41.0
15	38.58828441298988	40.0	38.0	41.0	34.0	41.0
16	38.54577178907972	40.0	38.0	41.0	34.0	41.0
17	38.535195311915636	40.0	38.0	41.0	34.0	41.0
18	38.4323816725168	40.0	38.0	41.0	34.0	41.0
19	38.2774037108108	40.0	37.0	41.0	34.0	41.0
20	38.30712310845343	40.0	37.0	41.0	34.0	41.0
21	38.239928411243675	40.0	37.0	41.0	34.0	41.0
22	38.29654119141424	40.0	37.0	41.0	34.0	41.0
23	38.313446963257725	40.0	37.0	41.0	34.0	41.0
24	38.26761057566118	40.0	37.0	41.0	34.0	41.0
25	38.02741833047513	40.0	36.0	41.0	34.0	41.0
26	38.05425595428329	40.0	36.0	41.0	34.0	41.0
27	38.027539367696114	40.0	36.0	41.0	34.0	41.0
28	37.96720843289438	40.0	36.0	41.0	34.0	41.0
29	37.908584258905414	40.0	36.0	41.0	34.0	41.0
30	37.75619159784091	40.0	36.0	41.0	33.0	41.0
31	37.7278049695979	40.0	36.0	41.0	33.0	41.0
32	37.617920852537225	40.0	36.0	41.0	33.0	41.0
33	37.48186277643065	40.0	36.0	41.0	33.0	41.0
34	37.353933233692956	40.0	36.0	41.0	33.0	41.0
35	37.315295160823105	40.0	36.0	41.0	33.0	41.0
36	37.204630421685515	40.0	35.0	41.0	32.0	41.0
37	37.144761876267324	40.0	35.0	41.0	32.0	41.0
38	37.03720602574965	40.0	35.0	41.0	32.0	41.0
39	36.94015593401976	40.0	35.0	41.0	31.0	41.0
40	36.84896866767939	40.0	35.0	41.0	31.0	41.0
41	36.74559064123888	39.0	35.0	41.0	31.0	41.0
42	36.74027996317204	40.0	35.0	41.0	31.0	41.0
43	36.67439899579906	39.0	35.0	41.0	31.0	41.0
44	36.63063112070947	39.0	35.0	41.0	31.0	41.0
45	36.601436671014035	39.0	35.0	41.0	31.0	41.0
46	36.48581076578482	39.0	35.0	41.0	31.0	41.0
47	36.40633827047331	39.0	35.0	41.0	31.0	41.0
48	36.37405941159591	39.0	35.0	41.0	31.0	41.0
49	36.33470463518157	39.0	35.0	41.0	31.0	41.0
50	36.219188887423144	39.0	35.0	41.0	30.0	41.0
51	35.25086528013317	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	21.0
9	30.0
10	38.0
11	25.0
12	22.0
13	19.0
14	19.0
15	40.0
16	42.0
17	92.0
18	173.0
19	328.0
20	580.0
21	1044.0
22	1556.0
23	2491.0
24	3434.0
25	5038.0
26	6714.0
27	7648.0
28	8197.0
29	8923.0
30	10306.0
31	12597.0
32	16630.0
33	23816.0
34	40890.0
35	57316.0
36	54595.0
37	83928.0
38	139765.0
39	248806.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37987599804708	20.42781897727628	23.18787560637608	13.004429418300557
2	34.03811448489143	25.05402475959152	24.026840343745707	16.881020411771345
3	31.883787948228708	24.521732981010754	27.064738593601888	16.529740477158644
4	28.712612758411066	24.745855835150024	27.986253435621116	18.55527797081779
5	23.02345538146444	37.075876737870104	22.617096711459507	17.283571169205956
6	24.609314970128285	34.47616042735659	26.687755249139478	14.226769353375646
7	76.04265406066276	6.851658685916571	12.362388159567857	4.743299093852805
8	76.55318633884166	7.518179382601375	11.02635483489299	4.902279443663973
9	70.15643720820171	8.74011132704393	13.342789649549648	7.760661815204722
10	38.021055036576364	26.367482602599445	20.92434357707147	14.687118783752725
11	30.044022189250537	25.700553915282104	26.249301316041784	18.006122579425575
12	27.611854031831427	22.41337338894699	30.335735491513116	19.639037087708466
13	24.214924025344377	24.40232772255549	31.80354979049681	19.579198461603323
14	20.096394586780285	27.847536620559193	30.615073077922133	21.44099571473839
15	19.11055322169803	26.599629272511905	35.120377636129476	19.169439869660593
16	21.189129497586737	24.51547712464522	33.19547783182898	21.099915545939066
17	20.844241416217084	25.498326558422217	30.17457919166176	23.48285283369894
18	21.594128198816556	25.8551823650129	31.315185003352326	21.23550443281822
19	23.30524091166867	26.951589191512166	28.947751359628782	20.795418537190386
20	24.891100500332513	25.606444076044017	30.599569433885797	18.902885989737676
21	23.881051691053173	26.900046374935233	29.739525180501857	19.47937675350974
22	21.79281963686114	25.319762658249367	29.58367275887346	23.30374494601604
23	21.68715006303455	26.55121438411774	30.09257307452221	21.6690624783255
24	21.496074450130624	25.523621977639394	30.859051476178106	22.121252096051876
25	21.386868957488737	27.01849965524791	29.441148031241205	22.153483356022146
26	20.801946387310945	27.870384095981155	29.63875149426569	21.688918022442206
27	22.24800118589277	27.602334250405608	29.296039362936227	20.853625200765393
28	19.550231126693333	27.242622509387182	31.128325293651258	22.078821070268226
29	21.025253260185146	27.086090103371223	30.357494991914987	21.531161644528645
30	21.921336686109687	27.07480236253776	29.786308106365876	21.217552844986677
31	22.038565994524767	27.31198091691815	28.016444742428714	22.63300834612837
32	23.547451350516994	28.846977673392622	27.882623814957206	19.72294716113318
33	22.65123192771494	27.458041563365704	27.671284667304043	22.219441841615318
34	21.264879758360745	27.05113890585072	29.13161913802459	22.55236219776394
35	22.07025326698499	27.42934622221074	27.85868836451515	22.641712146289123
36	21.889785410526976	28.484545994823957	27.543311605565535	22.082356989083532
37	22.110100352095916	27.69073222078821	28.729068380589982	21.470099046525892
38	21.443035667901064	28.320261766789834	26.582629662822942	23.65407290248616
39	21.948264067857	26.717266571559517	28.446602865998194	22.887866494585285
40	22.077325104615596	26.551486377872763	30.18273900431246	21.188449513199174
41	19.703227613893983	27.601926259773073	29.157866535384347	23.536979590948594
42	21.046060782444435	27.07371438751766	28.669365751362346	23.210859078675554
43	22.650823937082404	25.89203751881857	27.75397076883115	23.70316777526788
44	21.978183380909574	26.395497959366853	27.868752133451014	23.757566526272555
45	21.59848009889693	26.203742362075367	27.76648248156222	24.43129505746548
46	21.971111543278965	27.496392682824002	28.188752786236027	22.343742987661003
47	20.64364602188734	26.92697375668255	30.23727375219465	22.19210646923547
48	21.10086752408165	25.921276847483583	29.348670154533252	23.629185473901522
49	20.855529157050555	25.57965269117421	30.307720134745708	23.257098017029527
50	19.987053097260887	25.63758736099419	29.87715402054369	24.498205521201232
51	19.723491148643227	25.269579810447553	28.926399849859447	26.080529191049774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	479.0
1	1034.5
2	1590.0
3	6068.0
4	10546.0
5	6825.0
6	3104.0
7	2966.0
8	2828.0
9	2788.5
10	2749.0
11	2751.0
12	2753.0
13	2650.5
14	2548.0
15	2589.5
16	2631.0
17	2520.0
18	2409.0
19	2422.5
20	2436.0
21	3307.0
22	4178.0
23	3864.5
24	3551.0
25	4266.5
26	6020.0
27	7058.0
28	8990.5
29	10923.0
30	12462.5
31	14002.0
32	15703.0
33	17404.0
34	19032.5
35	20661.0
36	23119.5
37	25578.0
38	26778.5
39	27979.0
40	31692.0
41	35405.0
42	38330.0
43	41255.0
44	46226.0
45	51197.0
46	59413.0
47	67629.0
48	66927.5
49	66226.0
50	64472.5
51	62719.0
52	54426.0
53	46133.0
54	41857.0
55	37581.0
56	35918.5
57	34256.0
58	32443.0
59	30630.0
60	29754.0
61	28878.0
62	25653.5
63	22429.0
64	18356.0
65	14283.0
66	11944.0
67	9605.0
68	7888.0
69	6171.0
70	5091.0
71	4011.0
72	3442.0
73	2873.0
74	2298.5
75	1437.5
76	1151.0
77	784.5
78	418.0
79	316.0
80	214.0
81	149.5
82	85.0
83	57.0
84	29.0
85	17.0
86	5.0
87	4.0
88	3.0
89	4.0
90	5.0
91	4.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	735311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.104651105529463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9310565390425	22.684950684045603
2	10.43747506698986	6.493080407627646
3	4.010851893846586	3.7426844638215053
4	2.065593631051225	2.5699827687860837
5	1.3907887009620443	2.162999865246847
6	0.9150996410760593	1.7078313037479638
7	0.6909289687174277	1.5043773157461207
8	0.5443824667857785	1.3546261357871296
9	0.4539975973299	1.2709293180925676
>10	6.182749574156297	44.99111583285739
>50	0.3219478615765252	6.105294004908442
>100	0.05027678932082034	2.858401364621088
>500	0.002205122338632471	0.4996063622378463
>1k	0.002205122338632471	1.1983968095853446
>5k	4.410244677264942E-4	0.8557233628884364
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6238	0.8483485219179367	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2059	0.2800175707965745	TruSeq Adapter, Index 23 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	1991	0.27076978312577943	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	1923	0.26152199545498434	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	1683	0.2288827448521782	No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	1080	0.14687662771262772	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC	860	0.1169573146600554	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	816	0.11097345204954094	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15082053716046678	0.0
2	0.0	0.0	0.0	0.7181995101392472	0.0
3	0.0	0.0	0.0	0.9934571902229125	0.0
4	1.3599687751169234E-4	0.0	0.0	1.384856203701563	0.0
5	1.3599687751169234E-4	0.0	0.0	2.502342546215139	0.0
6	1.3599687751169234E-4	0.0	0.0	3.182190936896089	0.0
7	1.3599687751169234E-4	0.0	0.0	3.852111555518685	0.0
8	1.3599687751169234E-4	0.0	0.0	4.7947059135522245	0.0
9	1.3599687751169234E-4	0.0	0.0	5.165297404771587	0.0
10	1.3599687751169234E-4	0.0	0.0	6.228928983790532	0.0
11	1.3599687751169234E-4	0.0	0.0	7.388438361455221	0.0
12	1.3599687751169234E-4	0.0	0.0	8.538427957694092	0.0
13	1.3599687751169234E-4	0.0	0.0	8.960426268612872	0.0
14	1.3599687751169234E-4	0.0	0.0	9.155309794087128	0.0
15	1.3599687751169234E-4	0.0	0.0	9.484014247032889	0.0
16	1.3599687751169234E-4	0.0	0.0	10.07165675476091	0.0
17	1.3599687751169234E-4	0.0	0.0	10.770408711415985	0.0
18	1.3599687751169234E-4	0.0	0.0	11.51172769073222	0.0
19	1.3599687751169234E-4	0.0	0.0	12.013828162505389	0.0
20	1.3599687751169234E-4	0.0	0.0	12.485329336838426	0.0
21	2.719937550233847E-4	0.0	0.0	13.062228091243025	0.0
22	2.719937550233847E-4	0.0	0.0	13.657486424111703	0.0
23	2.719937550233847E-4	0.0	0.0	14.25029681318517	0.0
24	2.719937550233847E-4	0.0	0.0	14.719214046845485	0.0
25	2.719937550233847E-4	0.0	0.0	15.16569179571637	0.0
26	2.719937550233847E-4	0.0	0.0	15.555594843542393	0.0
27	2.719937550233847E-4	0.0	0.0	15.964265460465027	0.0
28	2.719937550233847E-4	0.0	0.0	16.373616061775223	0.0
29	2.719937550233847E-4	0.0	0.0	16.800646257161937	0.0
30	4.07990632535077E-4	0.0	0.0	17.29037101308154	0.0
31	4.07990632535077E-4	0.0	0.0	17.74296862144045	0.0
32	4.07990632535077E-4	0.0	0.0	18.18237453268073	0.0
33	4.07990632535077E-4	0.0	0.0	18.597572999723926	0.0
34	4.07990632535077E-4	0.0	0.0	19.025691170130735	0.0
35	4.07990632535077E-4	0.0	0.0	19.502632219564237	0.0
36	4.07990632535077E-4	0.0	0.0	19.948837974680103	0.0
37	4.07990632535077E-4	0.0	0.0	20.366348388640997	0.0
38	4.07990632535077E-4	0.0	0.0	20.78372280572438	0.0
39	4.07990632535077E-4	0.0	0.0	21.253184026894743	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGTC	25	3.8894294E-5	45.000004	19
CGGTCGA	25	3.8894294E-5	45.000004	36
AACGTTG	45	3.8380676E-10	45.0	1
CGCGGTA	20	7.031628E-4	45.0	31
CGACCGT	20	7.031628E-4	45.0	35
TTCGTGT	20	7.031628E-4	45.0	28
TGCGGAT	20	7.031628E-4	45.0	18
TCGGACG	20	7.031628E-4	45.0	1
TATTACG	20	7.031628E-4	45.0	1
GTATACG	20	7.031628E-4	45.0	1
GTCGACC	30	2.1644319E-6	44.999996	41
CGTTTTT	2220	0.0	41.655403	1
CGTTATT	330	0.0	41.59091	1
CGTTCAT	50	1.0804797E-9	40.500004	17
TGTAGCG	50	1.0804797E-9	40.500004	1
GCGAGAC	45	1.9266736E-8	40.0	21
ACACGAC	45	1.9266736E-8	40.0	26
TCACGTC	45	1.9266736E-8	40.0	38
ATGGGAC	345	0.0	39.782608	5
TAGGGCG	165	0.0	39.545456	5
>>END_MODULE