##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936042.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547671
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.116511555295055	33.0	31.0	34.0	30.0	34.0
2	32.25861876929763	34.0	31.0	34.0	30.0	34.0
3	32.12677136456011	33.0	31.0	34.0	30.0	34.0
4	35.795494010089996	37.0	35.0	37.0	35.0	37.0
5	32.338476932318855	37.0	35.0	37.0	10.0	37.0
6	34.02712029667446	37.0	35.0	37.0	19.0	37.0
7	35.61215948991274	37.0	35.0	37.0	32.0	37.0
8	35.857445437132874	37.0	35.0	37.0	35.0	37.0
9	37.82316755862553	39.0	38.0	39.0	35.0	39.0
10	37.45081627473429	39.0	37.0	39.0	35.0	39.0
11	37.37335553644432	39.0	37.0	39.0	34.0	39.0
12	37.32325428952784	39.0	37.0	39.0	35.0	39.0
13	37.28012985898468	39.0	37.0	39.0	34.0	39.0
14	38.55314595806607	40.0	38.0	41.0	34.0	41.0
15	38.70493416668036	40.0	38.0	41.0	35.0	41.0
16	38.64158043789063	40.0	38.0	41.0	35.0	41.0
17	38.610019519017804	40.0	38.0	41.0	35.0	41.0
18	38.453867376581925	40.0	38.0	41.0	34.0	41.0
19	38.230402924383434	40.0	37.0	41.0	34.0	41.0
20	38.20968062942898	40.0	36.0	41.0	34.0	41.0
21	38.12047561400914	40.0	36.0	41.0	34.0	41.0
22	38.15489043604646	40.0	36.0	41.0	34.0	41.0
23	38.16431032499438	40.0	36.0	41.0	34.0	41.0
24	38.11728939454527	40.0	36.0	41.0	34.0	41.0
25	37.843214265498816	40.0	35.0	41.0	34.0	41.0
26	37.90447732306439	40.0	35.0	41.0	34.0	41.0
27	37.88375685402367	40.0	35.0	41.0	34.0	41.0
28	37.79227127235146	40.0	35.0	41.0	34.0	41.0
29	37.746824279540085	40.0	35.0	41.0	34.0	41.0
30	37.54565971176126	40.0	35.0	41.0	33.0	41.0
31	37.45305849679826	40.0	35.0	41.0	33.0	41.0
32	37.23479789873848	40.0	35.0	41.0	33.0	41.0
33	36.997286692192944	40.0	35.0	41.0	32.0	41.0
34	36.74191257159864	40.0	35.0	41.0	31.0	41.0
35	36.62152642736241	40.0	35.0	41.0	31.0	41.0
36	36.48412824487694	40.0	35.0	41.0	30.0	41.0
37	36.412552426548054	40.0	35.0	41.0	30.0	41.0
38	36.300224039615024	40.0	35.0	41.0	30.0	41.0
39	36.22020884801277	40.0	35.0	41.0	29.0	41.0
40	36.133244228743166	39.0	35.0	41.0	29.0	41.0
41	36.02729558439282	39.0	35.0	41.0	29.0	41.0
42	35.98444321499586	39.0	35.0	41.0	28.0	41.0
43	35.90192469566583	39.0	35.0	41.0	28.0	41.0
44	35.81242753404872	39.0	35.0	41.0	27.0	41.0
45	35.81103436187054	39.0	35.0	41.0	27.0	41.0
46	35.67202572347267	39.0	35.0	41.0	27.0	41.0
47	35.600068289173606	39.0	35.0	41.0	26.0	41.0
48	35.564212821201046	39.0	35.0	41.0	26.0	41.0
49	35.521964829249676	39.0	35.0	41.0	27.0	41.0
50	35.3953121490822	39.0	35.0	41.0	26.0	41.0
51	34.448415198175546	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	4.0
9	16.0
10	18.0
11	17.0
12	17.0
13	12.0
14	11.0
15	18.0
16	41.0
17	77.0
18	171.0
19	307.0
20	639.0
21	1000.0
22	1597.0
23	2382.0
24	3742.0
25	5838.0
26	7984.0
27	8814.0
28	8059.0
29	7626.0
30	8064.0
31	9707.0
32	12013.0
33	17333.0
34	29753.0
35	41740.0
36	38819.0
37	59253.0
38	100957.0
39	181504.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.51277317951836	21.025396634110624	22.076940352876086	17.384889833494928
2	35.82680112695395	23.96986511975255	24.272966799410593	15.930366953882896
3	29.32015753983687	23.488371668392155	31.999503351464657	15.191967440306314
4	26.674043358147504	25.01720923693239	31.009310334123953	17.299437070796152
5	21.294353726963816	36.03312937876937	26.37075908711617	16.301757807150643
6	24.82767939145947	31.776741876053322	29.120950351579687	14.274628380907517
7	77.36213894838325	5.286020256686952	13.416996700573886	3.934844094355918
8	79.19225228284864	4.837210661145104	12.34116832916112	3.6293687268451316
9	73.34129431720869	7.179310206309992	14.115773886146975	5.3636215903343425
10	37.80444829103604	28.939819709278012	20.564718599305056	12.691013400380886
11	28.131122516985563	23.44181086820372	30.75623869074682	17.6708279240639
12	25.703387617748614	21.407560378402362	33.699611628149015	19.18944037570001
13	22.98971462794269	22.817713554305413	34.46302615986605	19.729545657885847
14	18.914275176155027	25.88451826005029	33.884759280663026	21.316447283131662
15	18.57922000617159	24.947641923709675	36.87797966297284	19.595158407145895
16	21.196119568134883	23.6881266307692	35.335630332809295	19.780123468286618
17	21.193380697535563	24.12780665764665	32.5167847119895	22.162027932828284
18	22.211875377735904	24.612038979606368	33.2473327965147	19.92875284614303
19	22.714184245651133	26.61689225830837	30.475960932749775	20.192962563290735
20	24.026103262725247	24.777831946551853	32.03510866925581	19.160956121467084
21	23.347776310960413	26.07623920200266	31.88556633453296	18.69041815250397
22	22.39793598711635	23.900845580649698	32.21039638761227	21.49082204462168
23	21.653693549594554	25.413797699713882	32.08075651257781	20.851752238113757
24	21.409386292135242	24.070107783687654	33.53217533884394	20.988330585333166
25	20.54919833257558	26.324928652420887	31.696949445926474	21.428923569077053
26	19.655778743077505	28.105924907471824	31.15867007747352	21.079626271977155
27	19.772272039235233	27.220721929771706	31.883192646680214	21.123813384312847
28	18.832656832295303	26.598633120979564	33.891880344221256	20.676829702503877
29	20.01639670532126	25.440273448840635	32.980384208767674	21.56294563707043
30	20.715539073640926	26.366742076903833	32.095363822440845	20.822355027014396
31	21.653693549594554	25.832114535916634	31.00145890507257	21.512733009416237
32	22.23123006330443	26.592242422914484	30.74053583264405	20.435991681137033
33	22.63037480531195	26.116774486872597	29.30317654212109	21.94967416569437
34	20.026074048105524	26.73283778034623	30.652709382092535	22.588378789455714
35	21.17749524805951	27.16137973345311	29.840725545080897	21.82039947340648
36	22.175539694451597	27.7918677454165	28.993318981651395	21.03927357848051
37	21.300927016402184	27.769591597875365	30.12027293758479	20.80920844813766
38	21.078530723737426	27.19570691163125	29.54218864975505	22.183573714876268
39	22.591482842801607	25.966501786656586	28.90859658444577	22.533418786096032
40	22.558251212863198	25.827001977464576	29.91230136340978	21.70244544626245
41	20.22509864498942	26.181594424389825	30.468839869191537	23.124467061429215
42	20.682124852329224	26.180864058896674	30.40931508149966	22.72769600727444
43	22.09227802823228	25.90679440759142	29.809867602995226	22.19105996118107
44	21.1260044807923	26.592972788407636	29.620702940268885	22.660319790531176
45	21.100624279905272	25.802352142070696	28.54761343945544	24.549410138568593
46	21.347852999337196	27.3468925687137	28.777313387051716	22.527941044897393
47	19.975496237704753	26.036799465372457	31.61715701579963	22.37054728112316
48	20.31164695592792	25.35317736378227	31.695306123566887	22.63986955672292
49	21.274451267275428	24.766511281408	30.938830064034796	23.02020738728178
50	20.08322514794466	24.902541854507543	31.240288421333247	23.773944576214554
51	19.924918427303982	25.033277277781735	29.744499891358135	25.297304403556147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	685.0
2	1067.0
3	7045.0
4	13023.0
5	8541.0
6	4059.0
7	3941.5
8	3824.0
9	3784.5
10	3745.0
11	3693.0
12	3641.0
13	3542.0
14	3443.0
15	3295.0
16	3147.0
17	2969.0
18	2791.0
19	2724.5
20	2658.0
21	2791.5
22	2925.0
23	3061.5
24	3198.0
25	3429.5
26	4584.5
27	5508.0
28	6314.0
29	7120.0
30	8201.0
31	9282.0
32	10999.0
33	12716.0
34	14850.0
35	16984.0
36	17875.5
37	18767.0
38	20358.5
39	21950.0
40	23433.0
41	24916.0
42	27577.5
43	30239.0
44	32501.5
45	34764.0
46	38611.5
47	42459.0
48	43806.5
49	45154.0
50	44483.5
51	43813.0
52	39425.0
53	35037.0
54	31995.5
55	28954.0
56	27186.0
57	25418.0
58	23991.5
59	22565.0
60	22183.5
61	21802.0
62	18602.0
63	15402.0
64	13451.5
65	11501.0
66	9389.0
67	7277.0
68	6400.5
69	5524.0
70	4389.5
71	3255.0
72	2873.5
73	2492.0
74	2012.5
75	1195.0
76	857.0
77	660.0
78	463.0
79	341.0
80	219.0
81	149.5
82	80.0
83	78.5
84	77.0
85	63.5
86	50.0
87	27.5
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	547671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.7809039520787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55948958756788	27.03586839600857
2	8.843069070300693	6.32826010092054
3	3.43145751943014	3.6834195575510424
4	1.9187439458325768	2.746175713378717
5	1.328583352919389	2.3768956671569668
6	0.960725929436178	2.0625385323256453
7	0.7442654895497064	1.8641344397497415
8	0.6252824998057778	1.789853845477296
9	0.5336247263047809	1.7184217570529081
>10	5.905880513013914	43.71130831655601
>50	0.11682737735977619	2.6007805263376316
>100	0.026880635498702548	1.5570279831239167
>500	0.004135482384415776	1.001025830917871
>1k	5.16935298051972E-4	0.19199275914500183
>5k	5.16935298051972E-4	1.3322965742981199
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7203	1.315205661793303	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1038	0.1895298454729208	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	858	0.1566633982810848	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	814	0.14862937785641378	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	775	0.14150831429818267	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	682	0.12452731658240074	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	644	0.1175888443974576	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	574	0.10480744826729917	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	549	0.10024266393509973	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.651827465759553E-4	0.0	0.0	0.12215362872965704	0.0
2	3.651827465759553E-4	0.0	0.0	0.5074214263672898	0.0
3	3.651827465759553E-4	0.0	0.0	0.7453379857615247	0.0
4	3.651827465759553E-4	0.0	0.0	1.0064436495633327	0.0
5	3.651827465759553E-4	0.0	0.0	1.7349832289823635	0.0
6	3.651827465759553E-4	0.0	0.0	2.2986427983223505	0.0
7	3.651827465759553E-4	0.0	0.0	2.7987605697581213	0.0
8	3.651827465759553E-4	0.0	0.0	3.5446463296395097	0.0
9	3.651827465759553E-4	0.0	0.0	3.8745889411708854	0.0
10	3.651827465759553E-4	0.0	0.0	4.679269123251003	0.0
11	3.651827465759553E-4	0.0	0.0	5.592408581064179	0.0
12	3.651827465759553E-4	0.0	0.0	6.442188832346427	0.0
13	3.651827465759553E-4	0.0	0.0	6.828369586850499	0.0
14	3.651827465759553E-4	0.0	0.0	7.014977970350813	0.0
15	3.651827465759553E-4	0.0	0.0	7.278457322005364	0.0
16	3.651827465759553E-4	0.0	0.0	7.731101336386261	0.0
17	3.651827465759553E-4	0.0	0.0	8.257694856948788	0.0
18	3.651827465759553E-4	0.0	0.0	8.852942733867597	0.0
19	5.477741198639329E-4	0.0	0.0	9.248253057036067	0.0
20	5.477741198639329E-4	0.0	0.0	9.662187700279912	0.0
21	5.477741198639329E-4	0.0	0.0	10.121405004099177	0.0
22	5.477741198639329E-4	0.0	0.0	10.642520783463064	0.0
23	5.477741198639329E-4	0.0	0.0	11.13679563095362	0.0
24	5.477741198639329E-4	0.0	0.0	11.543244027892658	0.0
25	5.477741198639329E-4	0.0	0.0	11.902949033269975	0.0
26	5.477741198639329E-4	0.0	0.0	12.236178289520533	0.0
27	5.477741198639329E-4	0.0	0.0	12.58949259683277	0.0
28	5.477741198639329E-4	0.0	0.0	12.951753881436117	0.0
29	5.477741198639329E-4	0.0	0.0	13.324240282943592	0.0
30	7.303654931519105E-4	0.0	0.0	13.770311007886122	0.0
31	7.303654931519105E-4	0.0	0.0	14.16945574989364	0.0
32	7.303654931519105E-4	0.0	0.0	14.536647001575764	0.0
33	7.303654931519105E-4	0.0	0.0	14.883205428076344	0.0
34	7.303654931519105E-4	0.0	0.0	15.23305049929611	0.0
35	7.303654931519105E-4	0.0	0.0	15.598781019991929	0.0
36	7.303654931519105E-4	0.0	0.0	15.966520045793917	0.0
37	9.129568664398882E-4	0.0	0.0	16.348683790085655	0.0
38	9.129568664398882E-4	0.0	0.0	16.736690458322606	0.0
39	9.129568664398882E-4	0.0	0.0	17.133278921104093	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACTA	35	1.2103737E-7	45.000004	9
CGTGCTA	20	7.030047E-4	45.000004	29
GCGAATG	20	7.030047E-4	45.000004	1
ATTACGA	20	7.030047E-4	45.000004	25
AGGCGAT	30	2.1634114E-6	45.000004	30
TCGATCA	25	3.888121E-5	45.000004	17
CATTACG	20	7.030047E-4	45.000004	24
TAGCACG	25	3.888121E-5	45.000004	1
TACGACG	20	7.030047E-4	45.000004	1
TCCACGT	20	7.030047E-4	45.000004	19
TCGTACA	25	3.888121E-5	45.000004	34
CGTTTAG	20	7.030047E-4	45.000004	11
TGTAACG	45	3.8380676E-10	45.0	1
CGTTTTT	3365	0.0	43.93016	1
CGAATAT	140	0.0	41.785717	14
TCACGAC	45	1.9252184E-8	40.0	25
CTCACGA	45	1.9252184E-8	40.0	24
ACGATAG	45	1.9252184E-8	40.0	1
CGTTATT	525	0.0	39.857143	1
TACGGTT	75	0.0	39.0	33
>>END_MODULE