##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936038.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	875334
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816690543266912	33.0	31.0	34.0	30.0	34.0
2	31.963977178996817	33.0	31.0	34.0	30.0	34.0
3	31.83298832217188	33.0	31.0	34.0	30.0	34.0
4	35.57340855033622	37.0	35.0	37.0	33.0	37.0
5	31.719067236049327	37.0	35.0	37.0	0.0	37.0
6	33.572873897278065	37.0	35.0	37.0	17.0	37.0
7	35.24321116282471	37.0	35.0	37.0	32.0	37.0
8	35.413460461949384	37.0	35.0	37.0	33.0	37.0
9	37.33796699317061	39.0	37.0	39.0	34.0	39.0
10	37.07859856923186	39.0	37.0	39.0	33.0	39.0
11	37.050813746524184	39.0	37.0	39.0	33.0	39.0
12	37.050652665154104	39.0	37.0	39.0	33.0	39.0
13	36.93942540790144	39.0	37.0	39.0	33.0	39.0
14	38.21617348349316	40.0	37.0	41.0	33.0	41.0
15	38.23409921241492	40.0	37.0	41.0	33.0	41.0
16	38.33963607034572	40.0	37.0	41.0	34.0	41.0
17	38.106063514041494	40.0	37.0	41.0	33.0	41.0
18	38.01206967854556	39.0	37.0	41.0	33.0	41.0
19	37.75586119127099	39.0	37.0	41.0	33.0	41.0
20	37.241007432591445	39.0	35.0	41.0	32.0	41.0
21	37.50967744883667	39.0	35.0	41.0	33.0	41.0
22	37.503039982452414	39.0	35.0	41.0	33.0	41.0
23	37.31253441543456	39.0	35.0	41.0	32.0	41.0
24	37.407832895786065	39.0	35.0	41.0	33.0	41.0
25	37.39596199850571	39.0	35.0	41.0	33.0	41.0
26	37.2008159171242	39.0	35.0	41.0	32.0	41.0
27	37.03314963202617	39.0	35.0	41.0	32.0	41.0
28	37.01398209140739	39.0	35.0	41.0	32.0	41.0
29	36.95419576984328	39.0	35.0	41.0	31.0	41.0
30	36.705872272755315	39.0	35.0	41.0	31.0	41.0
31	36.56659972079229	39.0	35.0	41.0	30.0	41.0
32	36.042371254858146	39.0	35.0	41.0	27.0	41.0
33	35.74349676809081	39.0	35.0	41.0	25.0	41.0
34	35.16612858634532	39.0	35.0	41.0	22.0	41.0
35	34.91619084829334	39.0	35.0	41.0	20.0	41.0
36	34.7127496475631	39.0	35.0	41.0	18.0	41.0
37	34.41741438125333	39.0	35.0	41.0	15.0	41.0
38	34.38532834323812	39.0	34.0	41.0	16.0	41.0
39	34.18064647323193	39.0	34.0	41.0	14.0	41.0
40	34.128965629119854	38.0	33.0	41.0	15.0	41.0
41	34.028776444191585	38.0	33.0	41.0	15.0	41.0
42	33.93525671343738	38.0	34.0	41.0	13.0	41.0
43	33.77430100967174	38.0	33.0	40.0	11.0	41.0
44	33.888605949271934	38.0	34.0	40.0	12.0	41.0
45	33.971260113282476	38.0	34.0	40.0	13.0	41.0
46	33.792587743649854	38.0	33.0	40.0	12.0	41.0
47	33.52643333858847	38.0	33.0	40.0	11.0	41.0
48	33.65134565777178	38.0	33.0	40.0	13.0	41.0
49	33.63274818526414	38.0	33.0	40.0	12.0	41.0
50	33.394723614071886	38.0	33.0	40.0	10.0	41.0
51	32.5353864924703	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	15.0
10	17.0
11	14.0
12	22.0
13	22.0
14	25.0
15	32.0
16	62.0
17	175.0
18	497.0
19	1465.0
20	3681.0
21	6288.0
22	8779.0
23	10858.0
24	13304.0
25	16424.0
26	19220.0
27	19273.0
28	17755.0
29	16424.0
30	16825.0
31	20106.0
32	24385.0
33	31551.0
34	45790.0
35	61516.0
36	63298.0
37	90816.0
38	148738.0
39	237749.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.788654387925064	16.176568030031966	19.945415121542176	17.089362460500794
2	33.82069015941344	32.5356949461577	19.82843120454592	13.815183689882948
3	24.947962720515825	32.03017362515337	30.28832422823745	12.733539426093355
4	22.709160160578705	19.641645360513817	42.54718770206573	15.102006776841753
5	26.889278835278873	31.654888305492534	26.293620492292085	15.162212366936506
6	20.199032597842653	37.04368846634542	30.455688914174473	12.301590021637455
7	64.50943297072888	4.628404700377228	26.83901230844455	4.023150020449337
8	64.14477216696713	13.718192141513983	17.432659990357966	4.704375701160928
9	59.57623033036532	6.329126938974151	18.11411415528244	15.980528575378084
10	37.20259923640576	24.28490153472846	25.30371263997514	13.208786588890643
11	28.704928632956104	21.845032867454023	32.53603767247702	16.91400082711285
12	21.91586297344785	18.905126500284464	35.78679681127432	23.392213714993364
13	24.58250222200897	20.913616973635207	38.82323775838709	15.68064304596874
14	17.038296238921372	30.029223130827777	33.73306646377269	19.199414166478167
15	14.817886658121356	23.829304014239135	40.50431035467604	20.848498972963466
16	16.968151585566197	24.1569503755138	34.15656195235191	24.718336086568097
17	17.071197965576566	28.233679943884276	35.289158195614476	19.405963894924678
18	17.623101581796206	24.119935933026706	34.68321806304793	23.573744422129153
19	17.778242362343974	25.971343510020173	32.18348653199807	24.06692759563778
20	23.474582273737795	24.804931603250875	36.524458092568096	15.196028030443237
21	18.886847763253797	30.918026718943853	33.836341327995946	16.358784189806403
22	17.112553608108446	21.715710802962068	39.04932288703512	22.122412701894362
23	21.52664011680113	25.36289005111192	35.09860236206979	18.011867470017158
24	19.01788345934238	27.260337197001373	33.165168952651214	20.556610391005034
25	16.49576047542995	32.780630022368605	31.248529132879565	19.47508036932188
26	17.661829655879927	24.044764626988098	38.06044321367615	20.232962503455823
27	23.628352149008265	24.91654614124437	32.43413371353107	19.0209679962163
28	16.06712409206086	28.833108276383644	35.240948026696095	19.858819604859402
29	20.80737181464447	21.93505564733005	37.28862354255632	19.96894899546916
30	18.529955422730065	29.496626430596777	35.348792575177015	16.62462557149614
31	18.94294063751665	26.673703980423475	30.009116520094043	24.374238861965832
32	24.714451854949083	26.4796066415791	29.632688779368788	19.17325272410303
33	24.732159381447538	25.030902489792467	29.14967315333347	21.08726497542652
34	21.150212376075874	27.77762545496919	28.658889064060116	22.413273104894817
35	22.984483637103096	26.347657008638986	30.05732668901242	20.610532665245497
36	23.364110156808714	31.12331978421951	28.076825531739885	17.435744527231893
37	17.581517455051443	27.15934717490695	32.8272408017968	22.431894568244807
38	20.626412318040884	28.3045100498781	27.01814393134506	24.050933700735946
39	21.96430162657911	27.773512739137292	28.372941071636653	21.889244562646944
40	21.04785144870415	26.52119076832386	30.588323999753236	21.842633783218748
41	19.647700192155224	28.826596476316467	28.28645979706032	23.239243534467985
42	20.133914597170907	27.517039210175774	31.233563416935706	21.115482775717613
43	24.3897757884419	25.276408776535586	28.308165797284236	22.025649637738283
44	20.749679550891432	24.894840141020456	31.13931367912134	23.21616662896677
45	19.82420424660758	24.62659967509545	29.46612378817686	26.083072290120114
46	24.650933243767522	27.208242796463978	28.013535404771204	20.127288554997293
47	18.157754639943153	27.57473147392881	34.38778797578981	19.879725910338227
48	19.816093057050225	27.28250016565105	29.969131782839465	22.93227499445926
49	19.742521140501797	23.558664464078856	35.105342646349854	21.593471749069497
50	20.865978015249038	24.18379727052759	31.54852890439535	23.40169580982802
51	19.0127425645525	23.79514562441308	30.51315269371463	26.67895911731979
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	476.0
1	928.0
2	1380.0
3	14763.0
4	28146.0
5	18531.0
6	8916.0
7	8537.5
8	8159.0
9	7977.0
10	7795.0
11	7581.5
12	7368.0
13	7084.0
14	6800.0
15	6512.5
16	6225.0
17	5858.5
18	5492.0
19	5371.0
20	5250.0
21	5504.0
22	5758.0
23	6106.0
24	6454.0
25	6681.5
26	8096.0
27	9283.0
28	10534.0
29	11785.0
30	14165.5
31	16546.0
32	17889.0
33	19232.0
34	22300.5
35	25369.0
36	26296.0
37	27223.0
38	29049.5
39	30876.0
40	34063.0
41	37250.0
42	41197.5
43	45145.0
44	50903.5
45	56662.0
46	89496.0
47	122330.0
48	99460.5
49	76591.0
50	73669.0
51	70747.0
52	61388.0
53	52029.0
54	46750.5
55	41472.0
56	37758.5
57	34045.0
58	31168.5
59	28292.0
60	25439.5
61	22587.0
62	19312.0
63	16037.0
64	12930.0
65	9823.0
66	7999.5
67	6176.0
68	4976.0
69	3776.0
70	3391.0
71	3006.0
72	2357.5
73	1709.0
74	1410.5
75	849.0
76	586.0
77	388.0
78	190.0
79	158.5
80	127.0
81	114.5
82	102.0
83	82.5
84	63.0
85	43.0
86	23.0
87	13.5
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	875334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.322504298499794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14020213860191	28.019253237665108
2	9.202213586525632	6.684948851045809
3	3.4716308940455507	3.7829498421532257
4	1.8667748475378485	2.712237496960992
5	1.216853097051628	2.209957592415027
6	0.870534502084375	1.8971995916371256
7	0.6686948011612374	1.7002068852694456
8	0.5165168342877632	1.5008947946931825
9	0.45451716329487485	1.4858281455768023
>10	4.39288454324092	32.22858784325191
>50	0.147468045469671	3.3608563441198496
>100	0.042452899450134626	2.8762276247711918
>500	0.003830336792492148	0.8540105080643708
>1k	0.003830336792492148	3.246654392794811
>5k	3.19194732707679E-4	0.6448556130673405
>10k+	0.001276778930830716	6.795331236513825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	17146	1.9587951570486237	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16476	1.8822529457327146	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG	13154	1.5027406681335354	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	11835	1.3520553297369917	No Hit
GAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT	5562	0.6354145960284875	No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	4973	0.5681259953343524	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCT	3526	0.40281766731327695	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTA	2555	0.2918885819584296	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTC	2548	0.291088887213338	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACGAAGT	2505	0.2861764766363468	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTT	2435	0.27817952918543093	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1901	0.21717424434558696	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	1699	0.19409733884437255	No Hit
GAATATGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	1650	0.18849947562873143	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG	1517	0.17330527547199126	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC	1378	0.15742562267660115	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	1316	0.15034261207721852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.427263193249662E-4	0.0	0.0	0.2042648863176799	0.0
2	3.427263193249662E-4	0.0	0.0	1.0367471159580228	0.0
3	3.427263193249662E-4	0.0	0.0	1.3091002977149293	0.0
4	4.5696842576662165E-4	0.0	0.0	2.245771328430062	0.0
5	4.5696842576662165E-4	0.0	0.0	4.965990124912319	0.0
6	4.5696842576662165E-4	0.0	0.0	5.859249155179623	0.0
7	4.5696842576662165E-4	0.0	0.0	6.842987933748717	0.0
8	4.5696842576662165E-4	0.0	0.0	7.952050303084309	0.0
9	4.5696842576662165E-4	0.0	0.0	8.2868939170648	0.0
10	4.5696842576662165E-4	0.0	0.0	10.689633899745697	0.0
11	4.5696842576662165E-4	0.0	0.0	11.83468253261041	0.0
12	5.712105322082771E-4	0.0	0.0	14.25775761023792	0.0
13	5.712105322082771E-4	0.0	0.0	14.736546278334899	0.0
14	5.712105322082771E-4	0.0	0.0	14.991077691486907	0.0
15	5.712105322082771E-4	0.0	0.0	15.592905108221547	0.0
16	5.712105322082771E-4	0.0	0.0	16.21746670413808	0.0
17	5.712105322082771E-4	0.0	0.0	16.892866037421143	0.0
18	5.712105322082771E-4	0.0	0.0	17.53513515983613	0.0
19	5.712105322082771E-4	0.0	0.0	18.372986768479233	0.0
20	5.712105322082771E-4	0.0	0.0	18.866398426200742	0.0
21	5.712105322082771E-4	0.0	0.0	19.40379329490229	0.0
22	5.712105322082771E-4	0.0	0.0	20.083990796655904	0.0
23	5.712105322082771E-4	0.0	0.0	20.66274130788933	0.0
24	5.712105322082771E-4	0.0	0.0	21.09743252289983	0.0
25	6.854526386499324E-4	0.0	0.0	21.478544189989194	0.0
26	6.854526386499324E-4	0.0	0.0	21.85725677284328	0.0
27	6.854526386499324E-4	0.0	0.0	22.26647199811729	0.0
28	7.996947450915879E-4	0.0	0.0	22.663006349576275	0.0
29	7.996947450915879E-4	0.0	0.0	23.074049448553353	0.0
30	7.996947450915879E-4	0.0	0.0	23.540842695473955	0.0
31	7.996947450915879E-4	0.0	0.0	23.976447847335987	0.0
32	7.996947450915879E-4	0.0	0.0	24.380979146245892	0.0
33	7.996947450915879E-4	0.0	0.0	24.789280434668367	0.0
34	7.996947450915879E-4	0.0	0.0	25.19735323887796	0.0
35	9.139368515332433E-4	0.0	0.0	25.60314120095872	0.0
36	9.139368515332433E-4	0.0	0.0	25.998990099779057	0.0
37	9.139368515332433E-4	0.0	0.0	26.408319567159506	0.0
38	0.0010281789579748987	0.0	0.0	26.810566023940577	0.0
39	0.0011424210644165541	0.0	0.0	27.22412244925937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	30	2.1649066E-6	45.000004	2
CGTCGAA	115	0.0	45.000004	42
GTCGATC	20	7.032365E-4	45.0	14
GTAGACG	20	7.032365E-4	45.0	1
TCCGTTG	25	3.8900405E-5	45.0	1
CACGTTA	20	7.032365E-4	45.0	44
CGATCGA	20	7.032365E-4	45.0	41
GCCGTAC	20	7.032365E-4	45.0	33
CCCGCGA	35	1.2114833E-7	45.0	41
TACGGGT	65	0.0	44.999996	4
CGTTTTT	7910	0.0	44.004425	1
TAATACG	645	0.0	43.953487	4
GTCGAAT	375	0.0	43.8	43
CGATGAA	3815	0.0	43.23067	19
CCGATGA	3760	0.0	43.085106	18
CAGTCGA	235	0.0	43.085106	41
GATGAAT	3945	0.0	42.88973	20
CCGTCGA	105	0.0	42.857147	41
CCCGTCG	105	0.0	42.857147	40
CCGTACA	105	0.0	42.857147	41
>>END_MODULE