##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936035.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	971605
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.004420520684846	33.0	31.0	34.0	30.0	34.0
2	32.123562558858794	33.0	31.0	34.0	30.0	34.0
3	32.01401289618724	33.0	31.0	34.0	30.0	34.0
4	35.700502776334005	37.0	35.0	37.0	33.0	37.0
5	31.805365349087335	37.0	35.0	37.0	0.0	37.0
6	33.72004775603254	37.0	35.0	37.0	17.0	37.0
7	35.47059041482907	37.0	35.0	37.0	32.0	37.0
8	35.76153889697974	37.0	35.0	37.0	35.0	37.0
9	37.733052011877255	39.0	37.0	39.0	35.0	39.0
10	37.30416167063776	39.0	37.0	39.0	34.0	39.0
11	37.280257923744735	39.0	37.0	39.0	34.0	39.0
12	37.34558797042008	39.0	37.0	39.0	34.0	39.0
13	37.34846877074531	39.0	37.0	39.0	34.0	39.0
14	38.613597089352155	40.0	38.0	41.0	34.0	41.0
15	38.75100889764874	40.0	38.0	41.0	35.0	41.0
16	38.65620288080032	40.0	38.0	41.0	35.0	41.0
17	38.65138919622686	40.0	38.0	41.0	35.0	41.0
18	38.50712583817498	40.0	38.0	41.0	35.0	41.0
19	38.28871403502452	40.0	37.0	41.0	34.0	41.0
20	38.28707962597969	40.0	37.0	41.0	34.0	41.0
21	38.19796213481816	40.0	37.0	41.0	34.0	41.0
22	38.254476870744796	40.0	37.0	41.0	34.0	41.0
23	38.25521894185394	40.0	37.0	41.0	34.0	41.0
24	38.20341599724168	40.0	37.0	41.0	34.0	41.0
25	37.91916056422106	40.0	36.0	41.0	34.0	41.0
26	37.99332959381642	40.0	36.0	41.0	34.0	41.0
27	37.97337292418215	40.0	36.0	41.0	34.0	41.0
28	37.890073641037255	40.0	36.0	41.0	34.0	41.0
29	37.834528434909245	40.0	36.0	41.0	34.0	41.0
30	37.63150868923071	40.0	36.0	41.0	33.0	41.0
31	37.56341723231149	40.0	36.0	41.0	33.0	41.0
32	37.404477128051006	40.0	36.0	41.0	33.0	41.0
33	37.16233037088117	40.0	36.0	41.0	32.0	41.0
34	36.95386087967847	40.0	36.0	41.0	31.0	41.0
35	36.866189449416176	40.0	36.0	41.0	31.0	41.0
36	36.72195490965979	40.0	36.0	41.0	30.0	41.0
37	36.64660227149922	40.0	35.0	41.0	30.0	41.0
38	36.5287673488712	40.0	35.0	41.0	30.0	41.0
39	36.467981329861416	40.0	35.0	41.0	30.0	41.0
40	36.40037257939183	40.0	35.0	41.0	30.0	41.0
41	36.29169158248465	40.0	35.0	41.0	29.0	41.0
42	36.251298624441	40.0	35.0	41.0	29.0	41.0
43	36.16034705461582	39.0	35.0	41.0	29.0	41.0
44	36.10947144158377	39.0	35.0	41.0	28.0	41.0
45	36.12250863262334	39.0	35.0	41.0	28.0	41.0
46	36.00580894499308	39.0	35.0	41.0	28.0	41.0
47	35.96117866828598	39.0	35.0	41.0	27.0	41.0
48	35.90125719814122	39.0	35.0	41.0	27.0	41.0
49	35.84812861193592	39.0	35.0	41.0	27.0	41.0
50	35.7244188739251	39.0	35.0	41.0	27.0	41.0
51	34.76921485583133	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	16.0
9	26.0
10	30.0
11	24.0
12	18.0
13	15.0
14	24.0
15	35.0
16	65.0
17	123.0
18	238.0
19	498.0
20	969.0
21	1616.0
22	2534.0
23	3929.0
24	6135.0
25	9829.0
26	13507.0
27	14544.0
28	14023.0
29	13676.0
30	14656.0
31	17401.0
32	21896.0
33	30083.0
34	47301.0
35	65747.0
36	70271.0
37	108759.0
38	185409.0
39	327944.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22015119312889	20.345202011105336	24.181122987222174	15.253523808543596
2	35.92169657422512	23.525403842096328	24.785175045414544	15.767724538264005
3	30.15886085394785	22.201820698740743	31.52587728552241	16.113441161788998
4	26.63232486452828	25.100220768728033	30.969169569938398	17.298284796805284
5	21.031077444023033	36.677970986151784	26.15301485686056	16.13793671296463
6	23.249262817708843	33.06600933506929	29.50725860817925	14.177469239042614
7	75.74178807231333	5.737825556681985	13.89597624549071	4.624410125513969
8	77.37218314026791	4.963642632551294	13.306847947468365	4.357326279712435
9	71.71422543111655	7.321596739415709	15.331847818815259	5.632330010652477
10	34.752600079250314	28.410722464375954	23.025921027578082	13.810756428795653
11	24.655904405596925	26.00264510783703	31.21052279475713	18.130927691808914
12	23.06564910637553	22.478373413063952	35.2598020800634	19.196175400497115
13	22.146551324869673	23.012129414731294	36.62681851163796	18.21450074876107
14	18.953484183387282	26.925242253796554	34.761142645416605	19.36013091739956
15	18.28582603012541	26.29329820245882	37.77090484301748	17.64997092439829
16	20.531388784536926	26.13510634465652	34.5936877640605	18.739817106746052
17	20.78931252926858	25.723725176383404	32.66677301990006	20.82018927444795
18	21.53076610350914	25.287436766998933	34.07485552256318	19.106941606928743
19	21.26316764528795	27.173800052490467	32.26722793727904	19.295804364942544
20	21.422079960477767	26.332408746352687	33.39700804339212	18.84850324977743
21	21.791674600274803	26.736791185718477	33.44496992090407	18.026564293102652
22	20.28416897813412	25.197688361010904	32.973996634434776	21.5441460264202
23	19.542406636441765	26.651674291507348	32.93262179589442	20.873297276156464
24	19.653357074119626	25.845791242325845	33.62940701210883	20.8714446714457
25	19.248048332398454	27.480406132121594	32.06447064393452	21.20707489154543
26	18.66705091060668	27.472378178374957	33.01732700016982	20.843243910848543
27	19.399653151229153	27.233186325718783	32.92222662501737	20.444933898034694
28	18.169420700799193	26.729792456811154	34.37446287328698	20.72632396910267
29	19.058876806932858	25.676277911291113	33.55128884680503	21.713556434971
30	19.941848796578856	25.986795045311624	32.62889754581337	21.44245861229615
31	19.5293354809825	26.65229182641094	32.16667267047823	21.651700022128335
32	19.073491799651094	26.58240745982163	32.31539565975885	22.028705080768418
33	19.59633801802173	26.908054198980036	31.495926842698424	21.99968094029981
34	18.649142398402642	25.68543801236099	32.84791659161902	22.817502997617346
35	18.62372054487163	26.184303291975645	31.25827882730122	23.9336973358515
36	19.74783991436849	26.865032600696786	31.180675274417073	22.20645221051765
37	19.181766252746744	27.507268900427643	32.68663705929879	20.624327787526823
38	18.596446086629857	26.774254969869443	31.27855455663567	23.35074438686503
39	20.854565384080978	24.728258911800577	31.69436139171783	22.722814312400615
40	20.211608626962605	24.783322440703785	32.21175271844011	22.793316213893505
41	19.178575655744876	25.788257573808288	30.816947216204117	24.21621955424272
42	20.721486612357904	26.225060595612415	30.518986625223217	22.534466166806467
43	20.31885385521894	25.840542195645348	31.861919195557864	21.97868475357784
44	19.522130907107314	26.117609522388214	31.118098404186888	23.242161166317587
45	19.545700155927566	24.704380895528534	31.3472038534178	24.40271509512611
46	19.865377391017955	26.322013575475626	30.725037438053533	23.087571595452886
47	18.882982281894392	25.54566927918238	32.56333592354918	23.008012515374045
48	19.27449941076878	24.523237323809575	32.0410043175982	24.16125894782345
49	20.159015237673746	23.892013729859357	32.740259673426955	23.20871135903994
50	18.396776467803274	23.95685489473603	32.765269837022245	24.88109880043845
51	18.704000082337988	23.730219585119467	31.096072992625608	26.469707339916944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	417.0
1	969.0
2	1521.0
3	11307.0
4	21093.0
5	13881.0
6	6669.0
7	6515.0
8	6361.0
9	6290.0
10	6219.0
11	6057.0
12	5895.0
13	5787.0
14	5679.0
15	5363.0
16	5047.0
17	4863.5
18	4680.0
19	4736.5
20	4793.0
21	5321.5
22	5850.0
23	6069.5
24	6289.0
25	7032.0
26	9376.5
27	10978.0
28	12623.5
29	14269.0
30	16978.5
31	19688.0
32	22625.5
33	25563.0
34	28518.5
35	31474.0
36	33856.0
37	36238.0
38	38359.5
39	40481.0
40	46167.0
41	51853.0
42	56555.5
43	61258.0
44	64588.0
45	67918.0
46	79117.5
47	90317.0
48	93999.5
49	97682.0
50	93025.5
51	88369.0
52	75108.0
53	61847.0
54	53255.0
55	44663.0
56	40394.0
57	36125.0
58	33650.0
59	31175.0
60	28445.5
61	25716.0
62	22126.0
63	18536.0
64	14946.5
65	11357.0
66	9464.0
67	7571.0
68	5847.5
69	4124.0
70	3373.5
71	2623.0
72	2116.0
73	1609.0
74	1209.5
75	700.0
76	590.0
77	434.0
78	278.0
79	208.5
80	139.0
81	93.0
82	47.0
83	25.0
84	3.0
85	5.5
86	8.0
87	5.0
88	2.0
89	1.5
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	971605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.53742206508043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23715846335274	28.242089722162095
2	10.089388904673097	7.5745929938690715
3	3.8386879740295288	4.322833519718847
4	2.0440931187741906	3.0691994455901352
5	1.284283342885665	2.410434294652581
6	0.9070693740504935	2.0429427561625007
7	0.6516767619013377	1.7123585963046806
8	0.542024774573606	1.6276970186319617
9	0.4284925773633686	1.4476056055418602
>10	4.765395843382782	38.348569814781676
>50	0.16987908706667987	3.9672662057723262
>100	0.03771986564959932	2.481453684287502
>500	0.002477947378169696	0.6483198234282249
>1k	0.001376637432316498	0.8696973241110335
>5k	0.0	0.0
>10k+	2.7532748646329956E-4	1.2349391949854711
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11949	1.2298207604942337	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2472	0.25442438027799363	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	1794	0.1846429361726216	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	1647	0.1695133310347312	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1380	0.14203302782509353	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1122	0.11547902697083691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.14612419656136E-4	0.0	0.0	0.17764420726529814	0.0
2	5.14612419656136E-4	0.0	0.0	0.7275590389098451	0.0
3	5.14612419656136E-4	0.0	0.0	1.0391053977696698	0.0
4	5.14612419656136E-4	0.0	0.0	1.4259910148671529	0.0
5	7.204573875185903E-4	0.0	0.0	2.459435676020605	0.0
6	8.233798714498175E-4	0.0	0.0	3.378533457526464	0.0
7	8.233798714498175E-4	0.0	0.0	4.210044205206849	0.0
8	8.233798714498175E-4	0.0	0.0	5.499662928865125	0.0
9	8.233798714498175E-4	0.0	0.0	6.017671790490992	0.0
10	8.233798714498175E-4	0.0	0.0	7.182960153560346	0.0
11	8.233798714498175E-4	0.0	0.0	8.634578866926374	0.0
12	8.233798714498175E-4	0.0	0.0	9.82600954091426	0.0
13	8.233798714498175E-4	0.0	0.0	10.348546991833102	0.0
14	8.233798714498175E-4	0.0	0.0	10.578578743419394	0.0
15	8.233798714498175E-4	0.0	0.0	10.913385583647676	0.0
16	8.233798714498175E-4	0.0	0.0	11.587630775881145	0.0
17	8.233798714498175E-4	0.0	0.0	12.433241903860107	0.0
18	8.233798714498175E-4	0.0	0.0	13.383319353029266	0.0
19	8.233798714498175E-4	0.0	0.0	13.962772937562075	0.0
20	8.233798714498175E-4	0.0	0.0	14.545005429161028	0.0
21	9.263023553810447E-4	0.0	0.0	15.234071459080594	0.0
22	9.263023553810447E-4	0.0	0.0	16.01206251511674	0.0
23	9.263023553810447E-4	0.0	0.0	16.76360249278256	0.0
24	9.263023553810447E-4	0.0	0.0	17.339350867893845	0.0
25	9.263023553810447E-4	0.0	0.0	17.851904837871356	0.0
26	9.263023553810447E-4	0.0	0.0	18.34253631877152	0.0
27	9.263023553810447E-4	0.0	0.0	18.872278343565544	0.0
28	9.263023553810447E-4	0.0	0.0	19.404902197909646	0.0
29	9.263023553810447E-4	0.0	0.0	19.93464422270367	0.0
30	0.001029224839312272	0.0	0.0	20.529947869761887	0.0
31	0.0011321473232434993	0.0	0.0	21.09005202731563	0.0
32	0.0012350698071747265	0.0	0.0	21.649435727481848	0.0
33	0.0013379922911059534	0.0	0.0	22.201203163837157	0.0
34	0.0013379922911059534	0.0	0.0	22.72404938220779	0.0
35	0.0013379922911059534	0.0	0.0	23.241646553897933	0.0
36	0.0015438372589684079	0.0	0.0	23.750289469486056	0.0
37	0.0015438372589684079	0.0	0.0	24.28805944802672	0.0
38	0.0015438372589684079	0.0	0.0	24.823359286953032	0.0
39	0.0015438372589684079	0.0	0.0	25.37100982395109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	30	2.165154E-6	45.000004	1
GTATGCG	25	3.8903578E-5	45.000004	1
GATGCGA	30	2.165154E-6	45.000004	11
TGCCGGT	25	3.8903578E-5	45.000004	36
ACGCAAA	20	7.0327485E-4	45.0	37
ATCGTCG	20	7.0327485E-4	45.0	24
TAGGCGT	20	7.0327485E-4	45.0	2
CGTTATT	810	0.0	44.166668	1
CGTTTTT	6030	0.0	43.208958	1
TCGTAAG	60	3.6379788E-12	41.250004	1
CGTAAGG	140	0.0	40.178574	2
TGCGAAG	85	0.0	39.705883	1
TTACGCG	40	3.4574805E-7	39.375	1
TTGTGCG	75	0.0	39.0	1
TAACGGG	320	0.0	38.671875	3
TATACGA	35	6.247292E-6	38.57143	43
TACGGGA	585	0.0	37.69231	4
TAGCGTA	30	1.1397918E-4	37.500004	14
CGGTCTA	30	1.1397918E-4	37.500004	31
CACGGGC	145	0.0	37.241383	4
>>END_MODULE