##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936034.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229475
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01362239895413	33.0	31.0	34.0	30.0	34.0
2	32.16148164288049	33.0	31.0	34.0	30.0	34.0
3	31.98391545920035	33.0	31.0	34.0	30.0	34.0
4	35.700773504739075	37.0	35.0	37.0	33.0	37.0
5	31.954387188146857	37.0	35.0	37.0	0.0	37.0
6	33.80458873515634	37.0	35.0	37.0	17.0	37.0
7	35.488946508334244	37.0	35.0	37.0	32.0	37.0
8	35.74503540690707	37.0	35.0	37.0	35.0	37.0
9	37.712138577187055	39.0	37.0	39.0	35.0	39.0
10	37.427392962196315	39.0	37.0	39.0	34.0	39.0
11	37.36637977993245	39.0	37.0	39.0	34.0	39.0
12	37.21981043686676	39.0	37.0	39.0	34.0	39.0
13	37.053661618912734	39.0	37.0	39.0	33.0	39.0
14	38.240710317028	40.0	37.0	41.0	33.0	41.0
15	38.42007190325744	40.0	37.0	41.0	34.0	41.0
16	38.43850528379998	40.0	37.0	41.0	34.0	41.0
17	38.414624686785054	40.0	37.0	41.0	34.0	41.0
18	38.19721538293932	39.0	37.0	41.0	34.0	41.0
19	37.92353851182046	39.0	37.0	41.0	34.0	41.0
20	37.79835276173875	39.0	35.0	41.0	34.0	41.0
21	37.712230090423795	39.0	35.0	41.0	34.0	41.0
22	37.72681555725025	39.0	35.0	41.0	34.0	41.0
23	37.73658568471511	39.0	35.0	41.0	34.0	41.0
24	37.69392744307659	39.0	35.0	41.0	34.0	41.0
25	37.43446998583724	39.0	35.0	41.0	33.0	41.0
26	37.44615753350038	39.0	35.0	41.0	33.0	41.0
27	37.40518139230853	39.0	35.0	41.0	33.0	41.0
28	37.32045756618368	39.0	35.0	41.0	33.0	41.0
29	37.232596143370735	39.0	35.0	41.0	33.0	41.0
30	37.01309946617278	39.0	35.0	41.0	33.0	41.0
31	36.82353633293387	39.0	35.0	41.0	32.0	41.0
32	36.50696590042488	39.0	35.0	41.0	31.0	41.0
33	36.14082579801721	39.0	35.0	41.0	30.0	41.0
34	35.810062098267785	39.0	35.0	41.0	27.0	41.0
35	35.642200675454845	39.0	35.0	41.0	25.0	41.0
36	35.45169190543632	39.0	35.0	41.0	24.0	41.0
37	35.37206667392962	39.0	35.0	41.0	23.0	41.0
38	35.257579256999676	39.0	35.0	41.0	23.0	41.0
39	35.186987689290774	39.0	35.0	41.0	23.0	41.0
40	35.09136507244798	39.0	35.0	41.0	23.0	41.0
41	34.99007735047391	38.0	35.0	41.0	22.0	41.0
42	34.952539492319424	38.0	35.0	41.0	22.0	41.0
43	34.82464320732106	38.0	35.0	41.0	21.0	41.0
44	34.789985837237175	38.0	35.0	41.0	21.0	41.0
45	34.79185096415731	38.0	35.0	41.0	20.0	41.0
46	34.67850528379998	38.0	35.0	41.0	20.0	41.0
47	34.57704325089879	38.0	34.0	40.0	20.0	41.0
48	34.548861531757275	38.0	35.0	40.0	20.0	41.0
49	34.55567273123434	38.0	35.0	40.0	20.0	41.0
50	34.43059592548208	38.0	34.0	40.0	20.0	41.0
51	33.57906089988016	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	10.0
11	9.0
12	7.0
13	7.0
14	4.0
15	9.0
16	22.0
17	36.0
18	80.0
19	158.0
20	288.0
21	516.0
22	863.0
23	1418.0
24	2187.0
25	3317.0
26	4690.0
27	4948.0
28	4427.0
29	4009.0
30	4094.0
31	4614.0
32	5750.0
33	8279.0
34	14383.0
35	22023.0
36	15849.0
37	22728.0
38	38567.0
39	66126.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.41714783745506	19.59952064495043	22.741039328902932	20.24229218869158
2	38.10131822638632	23.638740603551586	22.093038457348296	16.166902712713803
3	27.735047390783308	23.23477502995969	33.77535679267894	15.254820786578058
4	24.503322802048153	22.887024730362786	35.56509423684497	17.044558230744087
5	20.67240440135091	34.22900098049897	29.371391219087045	15.727203399063077
6	22.573265061553546	31.20775683625667	33.38664342520972	12.832334676980064
7	68.03660529469441	6.080836692450158	20.817954025492973	5.0646039873624575
8	68.5007081381414	7.095326288266696	18.843882775901513	5.56008279769038
9	62.998147946399385	7.868395250027237	20.83015579039111	8.303301013182264
10	34.871772524240114	24.52206122671315	27.84965682536224	12.756509423684498
11	27.737662054690055	22.02287830918401	33.089007517158734	17.15045211896721
12	25.699531539383376	19.345026691360715	36.922976359080515	18.032465410175398
13	20.881141736572612	25.265061553546136	38.05294694411156	15.800849765769692
14	16.067109706939753	29.22409848567382	36.651487090096964	18.057304717289465
15	14.357991066564985	24.367360278897486	45.08726440788757	16.187384246649962
16	16.526418999891053	22.487416929948797	41.33391436975705	19.652249700403093
17	16.685477720884627	23.04956966989868	37.06591131931583	23.19904128990086
18	18.62032901187493	23.279660093692122	37.85248937792788	20.247521516505067
19	19.578167556378688	25.149580564331625	35.77906089988016	19.493190979409523
20	21.779278788539056	25.099901950103497	36.27802592875041	16.842793332607037
21	19.640047935504956	25.120383484039653	36.90728837564004	18.33228020481534
22	17.20274539710208	24.500708138141412	35.96905981043687	22.327486654319642
23	16.77263318444275	25.754875258742782	35.84442749754875	21.628064059265714
24	18.786360169953152	22.858699204706394	37.886044231397754	20.468896393942696
25	16.871990412899006	26.200675454842575	35.71500163416494	21.212332498093474
26	16.213095108399607	27.109271162435995	36.66194574572393	20.01568798344046
27	18.031158078222028	27.822202854341434	35.72110251661401	18.425536550822528
28	14.957184878527071	26.392853251988235	39.151105784943894	19.4988560845408
29	16.986164070160147	25.682536223989544	37.55485346987689	19.77644623597342
30	18.96284998365835	27.76250136180412	35.33979736354723	17.934851290990302
31	21.633729164396993	26.549297309075065	31.53502560191742	20.281947924610524
32	22.169735265279442	29.963612593964484	30.028979191633077	17.837672949123
33	21.805425427606494	26.528815775138902	31.734611613465518	19.931147183789083
34	19.446562806405925	27.402113519991282	31.484911210371504	21.66641246323129
35	20.15687983440462	28.6218542324872	30.02244253186622	21.198823401241963
36	22.186730580673274	27.613029741801938	31.376402658241638	18.823837019283147
37	19.89759233031921	28.183898028107635	33.118640374768496	18.799869266804663
38	21.453753132149473	28.09238479137161	29.42891382503541	21.02494825144351
39	21.285978864800086	26.16014816428805	31.718052075389476	20.83582089552239
40	22.068634927552022	24.597450702690928	32.25013618041181	21.083778189345246
41	19.107092275847045	26.61292079747249	30.408977012746487	23.871009913933978
42	22.043795620437955	24.967425645495155	32.29807168536878	20.690707048698115
43	21.778843011221266	24.908159930275627	32.04401350909685	21.268983549406254
44	20.025275084431858	26.629916112866326	30.578494389367034	22.766314413334786
45	19.68406144460181	26.895304499400808	29.547445255474454	23.873188800522932
46	23.058720993572283	26.093038457348293	30.148817954025493	20.699422595053925
47	19.503649635036496	25.602788974833864	33.50648218760214	21.38707920252751
48	20.42836910338817	24.429240658023748	32.254058176271926	22.888332062316156
49	20.14293496023532	22.69092493735701	35.1972981806297	21.968841921777972
50	19.14979845299052	23.240440135090967	33.98801612376076	23.621745288157754
51	19.302320514217236	23.844863274866544	30.584595271816102	26.268220939100118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	484.0
1	526.5
2	569.0
3	4541.5
4	8514.0
5	5415.5
6	2317.0
7	2332.5
8	2348.0
9	2391.0
10	2434.0
11	2437.5
12	2441.0
13	2321.5
14	2202.0
15	2081.0
16	1960.0
17	1855.0
18	1750.0
19	1646.5
20	1543.0
21	1494.5
22	1446.0
23	1515.5
24	1585.0
25	1551.5
26	1893.0
27	2268.0
28	2483.5
29	2699.0
30	3216.0
31	3733.0
32	4208.0
33	4683.0
34	5263.5
35	5844.0
36	6295.5
37	6747.0
38	7715.0
39	8683.0
40	9755.0
41	10827.0
42	12212.0
43	13597.0
44	15603.0
45	17609.0
46	20707.0
47	23805.0
48	23583.0
49	23361.0
50	22717.5
51	22074.0
52	18391.5
53	14709.0
54	12430.0
55	10151.0
56	8436.5
57	6722.0
58	6290.5
59	5859.0
60	5652.5
61	5446.0
62	4581.5
63	3717.0
64	3016.5
65	2316.0
66	1801.0
67	1286.0
68	1038.0
69	790.0
70	737.5
71	685.0
72	493.5
73	302.0
74	257.5
75	156.0
76	99.0
77	73.0
78	47.0
79	41.0
80	35.0
81	44.0
82	53.0
83	27.0
84	1.0
85	2.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	229475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.79431310600283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9738715258766	30.6312234448197
2	9.70454893887295	7.723717180520754
3	3.6093650758886526	4.308966118313542
4	1.9590880220766989	3.1184224861095977
5	1.1257364375041066	2.2398954134437306
6	0.8191156179504588	1.955768602244253
7	0.5727238879519919	1.5953807604314196
8	0.4018922884863882	1.279442205033228
9	0.36246961168663355	1.2981806296982243
>10	4.313497886506494	36.00653665976686
>50	0.11826803039926412	2.9040200457566185
>100	0.03175715631091351	2.389367033445909
>500	0.0054753717777437086	1.8211134110469551
>1k	0.0010950743555487418	0.483712822747576
>5k	0.0010950743555487418	2.2442531866216364
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5150	2.2442531866216364	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	1110	0.483712822747576	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTC	999	0.43534154047281837	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	989	0.4309837672949123	No Hit
GCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	904	0.3939426952827106	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.281947924610524	No Hit
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	640	0.27889748338598974	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	449	0.19566401568798347	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	443	0.1930493517812398	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	382	0.16646693539601262	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	313	0.13639830046846063	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	284	0.12376075825253295	No Hit
ACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	283	0.12332498093474235	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2030722300904238	0.0
2	0.0	0.0	0.0	1.2005665105131278	0.0
3	0.0	0.0	0.0	1.6633620220067546	0.0
4	0.0	0.0	0.0	2.3009042379344153	0.0
5	0.0	0.0	0.0	4.222682209391001	0.0
6	0.0	0.0	0.0	5.117768820132912	0.0
7	0.0	0.0	0.0	6.147510622072121	0.0
8	0.0	0.0	0.0	7.56335112757381	0.0
9	0.0	0.0	0.0	8.103715001634164	0.0
10	0.0	0.0	0.0	9.756618368013944	0.0
11	0.0	0.0	0.0	11.55550713585358	0.0
12	0.0	0.0	0.0	13.448959581653774	0.0
13	0.0	0.0	0.0	14.08170824708574	0.0
14	0.0	0.0	0.0	14.352325961433708	0.0
15	0.0	0.0	0.0	14.815993027562916	0.0
16	0.0	0.0	0.0	15.67185967970367	0.0
17	0.0	0.0	0.0	16.704651922867416	0.0
18	0.0	0.0	0.0	17.856411373787996	0.0
19	0.0	0.0	0.0	18.557141300795294	0.0
20	0.0	0.0	0.0	19.182481751824817	0.0
21	0.0	0.0	0.0	19.952936049678613	0.0
22	0.0	0.0	0.0	20.733849003159385	0.0
23	0.0	0.0	0.0	21.42412027453971	0.0
24	0.0	0.0	0.0	21.98932345571413	0.0
25	0.0	0.0	0.0	22.48175182481752	0.0
26	0.0	0.0	0.0	22.92580891164615	0.0
27	0.0	0.0	0.0	23.393397973635473	0.0
28	0.0	0.0	0.0	23.84355594291317	0.0
29	0.0	0.0	0.0	24.339470530558884	0.0
30	0.0	0.0	0.0	24.90772415295784	0.0
31	0.0	0.0	0.0	25.40930384573483	0.0
32	0.0	0.0	0.0	25.88125068090206	0.0
33	4.35777317790609E-4	0.0	0.0	26.314413334785925	0.0
34	4.35777317790609E-4	0.0	0.0	26.782002396775248	0.0
35	4.35777317790609E-4	0.0	0.0	27.28358208955224	0.0
36	4.35777317790609E-4	0.0	0.0	27.78603333696481	0.0
37	4.35777317790609E-4	0.0	0.0	28.204815339361588	0.0
38	4.35777317790609E-4	0.0	0.0	28.68722083015579	0.0
39	4.35777317790609E-4	0.0	0.0	29.160039219958602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.0214743E-4	45.000004	13
ACTATTC	20	7.0214743E-4	45.000004	10
CGGGTCA	20	7.0214743E-4	45.000004	7
CGTGGCG	25	3.8810173E-5	45.000004	5
CGGCGGA	20	7.0214743E-4	45.000004	44
GTACCTA	20	7.0214743E-4	45.000004	24
CGGCGAA	40	6.7739165E-9	45.000004	31
CGAACAC	25	3.8810173E-5	45.000004	34
CCGATTC	20	7.0214743E-4	45.000004	11
CAACGAG	20	7.0214743E-4	45.000004	14
GCGAACA	25	3.8810173E-5	45.000004	33
GGCACCG	25	3.8810173E-5	45.000004	8
TTTCGCG	20	7.0214743E-4	45.000004	1
CGGCAGG	20	7.0214743E-4	45.000004	2
CCAATCA	20	7.0214743E-4	45.000004	41
CGACCCG	25	3.8810173E-5	45.000004	10
TTCGTTG	20	7.0214743E-4	45.000004	1
GTTAGCG	20	7.0214743E-4	45.000004	1
ATCACTA	20	7.0214743E-4	45.000004	25
TGTTGCG	20	7.0214743E-4	45.000004	1
>>END_MODULE