##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936033.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339737
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85829332689698	33.0	31.0	34.0	30.0	34.0
2	31.99308288470199	33.0	31.0	34.0	30.0	34.0
3	31.834471959191962	33.0	31.0	34.0	30.0	34.0
4	35.589873931894374	37.0	35.0	37.0	33.0	37.0
5	31.72935535428876	37.0	35.0	37.0	0.0	37.0
6	33.65855352817032	37.0	35.0	37.0	17.0	37.0
7	35.40438927758825	37.0	35.0	37.0	32.0	37.0
8	35.70003267233184	37.0	35.0	37.0	33.0	37.0
9	37.70262585470526	39.0	37.0	39.0	35.0	39.0
10	37.28776671366381	39.0	37.0	39.0	34.0	39.0
11	37.29930505067155	39.0	37.0	39.0	34.0	39.0
12	37.24160747872619	39.0	37.0	39.0	34.0	39.0
13	37.13886918410417	39.0	37.0	39.0	33.0	39.0
14	38.304335412392525	40.0	38.0	41.0	33.0	41.0
15	38.49637513723851	40.0	38.0	41.0	34.0	41.0
16	38.462790334876686	40.0	38.0	41.0	34.0	41.0
17	38.47294524882483	40.0	38.0	41.0	34.0	41.0
18	38.29710629104278	40.0	37.0	41.0	34.0	41.0
19	38.03469742771615	40.0	37.0	41.0	34.0	41.0
20	37.97935167497211	40.0	35.0	41.0	34.0	41.0
21	37.86689115403974	39.0	35.0	41.0	34.0	41.0
22	37.93988879633363	40.0	35.0	41.0	34.0	41.0
23	37.96940280275625	40.0	35.0	41.0	34.0	41.0
24	37.886135451834804	40.0	35.0	41.0	34.0	41.0
25	37.63677786052152	39.0	35.0	41.0	33.0	41.0
26	37.69447543246688	39.0	35.0	41.0	34.0	41.0
27	37.6305024180469	39.0	35.0	41.0	33.0	41.0
28	37.567636142074605	39.0	35.0	41.0	33.0	41.0
29	37.45731551170464	39.0	35.0	41.0	33.0	41.0
30	37.20615064005393	39.0	35.0	41.0	33.0	41.0
31	37.09482040519578	39.0	35.0	41.0	33.0	41.0
32	36.79161233542417	39.0	35.0	41.0	31.0	41.0
33	36.52210091924047	39.0	35.0	41.0	31.0	41.0
34	36.28177972961438	39.0	35.0	41.0	30.0	41.0
35	36.07529353588217	39.0	35.0	41.0	30.0	41.0
36	35.958520855838486	39.0	35.0	41.0	29.0	41.0
37	35.830047948854556	39.0	35.0	41.0	27.0	41.0
38	35.67044507957626	39.0	35.0	41.0	26.0	41.0
39	35.62962821241137	39.0	35.0	41.0	26.0	41.0
40	35.61698313695594	39.0	35.0	41.0	25.0	41.0
41	35.54747643029756	39.0	35.0	41.0	25.0	41.0
42	35.52347551194012	39.0	35.0	41.0	25.0	41.0
43	35.39683931982681	39.0	35.0	41.0	24.0	41.0
44	35.28315726576734	39.0	35.0	41.0	23.0	41.0
45	35.25474999779241	39.0	35.0	41.0	23.0	41.0
46	35.124858346309054	39.0	35.0	40.0	23.0	41.0
47	35.05126023953823	38.0	35.0	40.0	23.0	41.0
48	35.03793228291296	38.0	35.0	40.0	23.0	41.0
49	35.04358665673742	38.0	35.0	40.0	23.0	41.0
50	34.91969670657008	38.0	35.0	40.0	23.0	41.0
51	33.96415462548972	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	11.0
11	11.0
12	2.0
13	2.0
14	7.0
15	11.0
16	22.0
17	43.0
18	86.0
19	183.0
20	354.0
21	566.0
22	855.0
23	1535.0
24	2394.0
25	4058.0
26	5673.0
27	6339.0
28	6136.0
29	5813.0
30	6124.0
31	7001.0
32	8845.0
33	12299.0
34	20011.0
35	28628.0
36	26008.0
37	38140.0
38	65045.0
39	93461.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.842539964737426	20.990354303475925	25.79819095359057	18.368914778196075
2	36.80317422005845	23.149377312450515	24.75561978824797	15.29182867924306
3	27.060343736478508	22.887704312453458	35.196931744261	14.85502020680703
4	25.520917651006513	23.2291448973765	34.2771025822916	16.97283486932539
5	19.571904149386143	34.59411250467273	29.928150304500246	15.905833041440879
6	20.613592278733258	30.729358297742078	33.071758448446886	15.58529097507778
7	69.96941752002283	5.253181137173755	19.45387167132223	5.323529671481175
8	70.6110903434127	4.641531537630579	18.26471653072818	6.482661588228542
9	66.58827269328921	5.708239020183259	20.289223723056367	7.414264563471155
10	32.819798844400225	24.455387549781154	28.13911937763623	14.585694228182389
11	24.050074027850954	24.510135781501575	33.540944907384244	17.898845283263228
12	22.27222822359649	20.791965549822365	39.25359910754496	17.682207119036196
13	19.59074225062328	24.470693507036327	39.83463679257779	16.10392744976261
14	15.503168627497152	27.517462036810826	37.874296882588595	19.105072453103432
15	14.070884242811351	25.154163367546072	44.11736137070734	16.657591018935236
16	15.90759911343186	24.47687475900476	39.80284749674012	19.812678630823257
17	16.183695034688597	24.330879474416975	36.160324015341274	23.325101475553147
18	16.97548397731186	23.934985003105343	39.55765783532556	19.531873184257233
19	17.27777663310149	26.583209953581743	35.99666801084368	20.14234540247309
20	18.68356993792257	26.412195315788388	36.74930902433353	18.15492572195551
21	18.104592670212547	27.664340357394103	36.85174119981044	17.37932577258291
22	15.942626207919655	24.697633757877416	35.730579830869175	23.629160203333754
23	15.467258497013866	25.636889711747617	35.92837989385907	22.967471897379443
24	18.378039483482812	23.235031804013104	37.09575347989769	21.2911752326064
25	16.50865228102915	26.102249681371177	34.96469327744697	22.424404760152704
26	17.1570950470511	26.150816661123162	36.11852697822139	20.573561313604348
27	18.74008424163397	27.92100948675004	34.78484827969871	18.55405799191728
28	16.558690987440286	25.04260648678242	37.34094314131225	21.05775938446504
29	18.709177981791797	22.240438927758827	38.229866043439486	20.820517047009893
30	18.994398608335274	25.988632383284717	36.51589317619217	18.501075832187837
31	20.680997359722372	26.481660814100316	31.91086045970854	20.926481366468767
32	20.451408000894812	27.261381598118543	33.03614266329543	19.251067737691212
33	18.701525003164214	25.40170779161528	33.19626652381106	22.700500681409444
34	19.293747810806593	23.18646482426112	33.7899610581126	23.72982630681969
35	17.656599075166966	25.482064067204924	33.07323017510604	23.788106682522066
36	21.330323161739816	25.0726297106291	32.152812322472975	21.444234805158107
37	18.48959636424646	26.154348805105126	34.67388008959872	20.682174741049693
38	17.550929101039923	27.11362024153978	33.114732866894094	22.2207177905262
39	18.623229144897376	22.34110503124476	35.54720268913895	23.488463134718916
40	19.740858369856685	23.937634111091814	33.66545298274842	22.65605453630308
41	17.333407900817395	25.750507009834077	29.826012474355164	27.09007261499336
42	20.177961187624543	25.060561552024062	31.135849200999598	23.62562805935179
43	21.189037402461317	23.641228361938794	32.32353261493449	22.846201620665397
44	20.037558464341533	23.36572113134572	32.4774752234817	24.119245180831054
45	19.993112319235173	22.801755475559034	31.39428440234652	25.81084780285927
46	22.035868922136828	23.913203448549908	31.866119969270347	22.184807660042914
47	17.117947117917684	24.0271150919682	35.186335312315116	23.668602477799002
48	18.371563886182546	23.1696871403468	33.4187915946747	25.03995737879595
49	19.944250994151357	19.585444034650333	36.77226796021629	23.698037010982024
50	18.949069427233418	20.29952580967042	35.36382554740873	25.387579215687428
51	17.319279324889546	21.00065639008998	30.965717599201735	30.71434668581874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	546.0
1	561.5
2	577.0
3	5858.0
4	11139.0
5	6983.5
6	2828.0
7	2913.0
8	2998.0
9	2999.0
10	3000.0
11	2920.5
12	2841.0
13	2807.5
14	2774.0
15	2582.0
16	2390.0
17	2296.5
18	2203.0
19	2145.5
20	2088.0
21	2071.0
22	2054.0
23	2256.5
24	2459.0
25	2463.5
26	2787.0
27	3106.0
28	3603.0
29	4100.0
30	4765.0
31	5430.0
32	6195.5
33	6961.0
34	7549.5
35	8138.0
36	9026.0
37	9914.0
38	11212.5
39	12511.0
40	13714.5
41	14918.0
42	17908.5
43	20899.0
44	24775.0
45	28651.0
46	33551.0
47	38451.0
48	42027.5
49	45604.0
50	41844.5
51	38085.0
52	29931.5
53	21778.0
54	17611.5
55	13445.0
56	11076.5
57	8708.0
58	7468.5
59	6229.0
60	5407.0
61	4585.0
62	4034.5
63	3484.0
64	2749.5
65	2015.0
66	1512.5
67	1010.0
68	789.5
69	569.0
70	474.5
71	380.0
72	283.5
73	187.0
74	140.5
75	79.5
76	65.0
77	46.5
78	28.0
79	22.5
80	17.0
81	10.5
82	4.0
83	4.0
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.97117033132568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73908963206685	29.47437711760236
2	11.554451331312004	9.236898844977699
3	4.562893936113241	5.471526321724663
4	2.2261041909671704	3.5591995916970687
5	1.36761617143716	2.7332609468195113
6	0.9124323219030092	2.1882592652755295
7	0.6624382761410803	1.8534903218737415
8	0.5262400939868236	1.6827545945536129
9	0.4005518053884748	1.4409471995732468
>10	3.883388024160698	30.698787560154667
>50	0.10567521508007106	2.8194208040382445
>100	0.0509901387449294	3.347859129698215
>500	0.00443392510825473	1.4435316363891986
>1k	0.00295595007216982	1.9704930522513493
>5k	7.38987518042455E-4	2.079193613370896
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7039	2.071896790752847	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	2289	0.6737564645593502	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1556	0.4580013363278065	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	1495	0.4400462710861637	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1331	0.3917736366660093	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	872	0.256669129355943	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	857	0.25225394937848983	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	844	0.24842746006469713	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	811	0.23871406411430018	No Hit
ACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	761	0.2239967975227897	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	742	0.21840423621801572	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	434	0.12774587401431106	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	380	0.11185122609547973	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	377	0.11096819009998912	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.830359954906295E-4	0.0	0.0	0.4580013363278065	0.0
2	8.830359954906295E-4	0.0	0.0	2.2944218616164855	0.0
3	8.830359954906295E-4	0.0	0.0	3.0544215084020876	0.0
4	8.830359954906295E-4	0.0	0.0	3.9380461945563776	0.0
5	8.830359954906295E-4	0.0	0.0	6.391120190029346	0.0
6	8.830359954906295E-4	0.0	0.0	7.918478116896305	0.0
7	8.830359954906295E-4	0.0	0.0	9.492048260860606	0.0
8	8.830359954906295E-4	0.0	0.0	11.734076653411314	0.0
9	8.830359954906295E-4	0.0	0.0	12.49701975351522	0.0
10	8.830359954906295E-4	0.0	0.0	14.591286789487162	0.0
11	8.830359954906295E-4	0.0	0.0	17.312215036925622	0.0
12	8.830359954906295E-4	0.0	0.0	19.54070354421214	0.0
13	8.830359954906295E-4	0.0	0.0	20.274506456464852	0.0
14	8.830359954906295E-4	0.0	0.0	20.547953269735117	0.0
15	8.830359954906295E-4	0.0	0.0	21.06776712574727	0.0
16	8.830359954906295E-4	0.0	0.0	22.263692208973413	0.0
17	8.830359954906295E-4	0.0	0.0	23.679787600408552	0.0
18	8.830359954906295E-4	0.0	0.0	25.30486817744314	0.0
19	8.830359954906295E-4	0.0	0.0	26.27355866449636	0.0
20	8.830359954906295E-4	0.0	0.0	27.168074127928367	0.0
21	0.0011773813273208394	0.0	0.0	28.277461683596428	0.0
22	0.0011773813273208394	0.0	0.0	29.425997168397906	0.0
23	0.0011773813273208394	0.0	0.0	30.504478464223798	0.0
24	0.0011773813273208394	0.0	0.0	31.33806444396695	0.0
25	0.0011773813273208394	0.0	0.0	32.12220040796263	0.0
26	0.001471726659151049	0.0	0.0	32.805375923140545	0.0
27	0.001766071990981259	0.0	0.0	33.45793952380812	0.0
28	0.001766071990981259	0.0	0.0	34.07223823133777	0.0
29	0.001766071990981259	0.0	0.0	34.73186611996927	0.0
30	0.001766071990981259	0.0	0.0	35.49686963739599	0.0
31	0.001766071990981259	0.0	0.0	36.20800795909777	0.0
32	0.0020604173228114687	0.0	0.0	36.8932438915985	0.0
33	0.002354762654641679	0.0	0.0	37.50665956313266	0.0
34	0.002354762654641679	0.0	0.0	38.15627971048193	0.0
35	0.002354762654641679	0.0	0.0	38.81649628977709	0.0
36	0.002354762654641679	0.0	0.0	39.436976249275176	0.0
37	0.0026491079864718885	0.0	0.0	40.063343115409864	0.0
38	0.0026491079864718885	0.0	0.0	40.64290907378354	0.0
39	0.0026491079864718885	0.0	0.0	41.219531578838925	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGCT	25	3.8849845E-5	45.000004	5
TTGTGCG	50	2.1827873E-11	45.000004	1
ACGGGTC	55	1.8189894E-12	45.000004	5
TGTGACG	25	3.8849845E-5	45.000004	1
TCTACGG	25	3.8849845E-5	45.000004	2
GCCCCCG	20	7.026263E-4	45.0	15
TGCCCCC	20	7.026263E-4	45.0	14
CAACGCT	20	7.026263E-4	45.0	15
ACGCATG	20	7.026263E-4	45.0	1
CCAGCGG	20	7.026263E-4	45.0	2
GCGCCAA	20	7.026263E-4	45.0	33
TTCGTGC	20	7.026263E-4	45.0	29
GTCTACG	20	7.026263E-4	45.0	1
CACGACG	20	7.026263E-4	45.0	26
CGCTGAT	20	7.026263E-4	45.0	18
TTATCCG	35	1.2085547E-7	45.0	1
CGTTATC	20	7.026263E-4	45.0	19
ACGATCT	20	7.026263E-4	45.0	12
CTAACGG	20	7.026263E-4	45.0	2
CCACTCG	20	7.026263E-4	45.0	35
>>END_MODULE