##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936032.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671014
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.004825532701254	33.0	31.0	34.0	30.0	34.0
2	32.11962343557661	33.0	31.0	34.0	30.0	34.0
3	32.022188806790915	33.0	31.0	34.0	30.0	34.0
4	35.713545469990194	37.0	35.0	37.0	33.0	37.0
5	31.94441844730512	37.0	35.0	37.0	0.0	37.0
6	33.784901656299276	37.0	35.0	37.0	17.0	37.0
7	35.48364117589201	37.0	35.0	37.0	32.0	37.0
8	35.7369712107348	37.0	35.0	37.0	35.0	37.0
9	37.72231875937015	39.0	37.0	39.0	35.0	39.0
10	37.297399160077134	39.0	37.0	39.0	34.0	39.0
11	37.26155788105763	39.0	37.0	39.0	34.0	39.0
12	37.23774168646258	39.0	37.0	39.0	34.0	39.0
13	37.17251651977455	39.0	37.0	39.0	34.0	39.0
14	38.320638615587754	40.0	38.0	41.0	33.0	41.0
15	38.47281576837443	40.0	38.0	41.0	34.0	41.0
16	38.46203804987675	40.0	38.0	41.0	34.0	41.0
17	38.45540927611049	40.0	38.0	41.0	34.0	41.0
18	38.32290384403306	40.0	38.0	41.0	34.0	41.0
19	38.132363855299594	40.0	37.0	41.0	34.0	41.0
20	38.104152819464275	40.0	36.0	41.0	34.0	41.0
21	38.0629822924708	40.0	36.0	41.0	34.0	41.0
22	38.12867689794848	40.0	36.0	41.0	34.0	41.0
23	38.14228168115717	40.0	36.0	41.0	34.0	41.0
24	38.11307662731329	40.0	36.0	41.0	34.0	41.0
25	37.85195688912601	40.0	36.0	41.0	34.0	41.0
26	37.889871150229354	40.0	36.0	41.0	34.0	41.0
27	37.8689416316202	40.0	36.0	41.0	34.0	41.0
28	37.812462929238436	40.0	36.0	41.0	34.0	41.0
29	37.7580199518937	40.0	36.0	41.0	33.0	41.0
30	37.58329483438497	40.0	35.0	41.0	33.0	41.0
31	37.509138408438574	40.0	35.0	41.0	33.0	41.0
32	37.32441648013305	40.0	35.0	41.0	33.0	41.0
33	37.134585865570614	40.0	35.0	41.0	32.0	41.0
34	36.90922693118176	40.0	35.0	41.0	31.0	41.0
35	36.823303239574734	40.0	35.0	41.0	31.0	41.0
36	36.70768121082421	40.0	35.0	41.0	31.0	41.0
37	36.61774269985425	40.0	35.0	41.0	30.0	41.0
38	36.48214940373823	40.0	35.0	41.0	30.0	41.0
39	36.397987225303794	40.0	35.0	41.0	30.0	41.0
40	36.33333134629084	39.0	35.0	41.0	30.0	41.0
41	36.23319930731699	39.0	35.0	41.0	29.0	41.0
42	36.21258274790094	39.0	35.0	41.0	30.0	41.0
43	36.13378111335978	39.0	35.0	41.0	29.0	41.0
44	36.08406083926714	39.0	35.0	41.0	29.0	41.0
45	36.076441028056045	39.0	35.0	41.0	29.0	41.0
46	35.97517041373205	39.0	35.0	41.0	29.0	41.0
47	35.892021626970525	39.0	35.0	41.0	28.0	41.0
48	35.84528191662171	39.0	35.0	41.0	28.0	41.0
49	35.821374814832474	39.0	35.0	41.0	28.0	41.0
50	35.70910741057563	39.0	35.0	41.0	28.0	41.0
51	34.713980632296796	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	18.0
9	26.0
10	23.0
11	16.0
12	12.0
13	11.0
14	23.0
15	20.0
16	33.0
17	80.0
18	173.0
19	365.0
20	685.0
21	1174.0
22	1776.0
23	2734.0
24	4063.0
25	6371.0
26	8580.0
27	9351.0
28	9364.0
29	9327.0
30	10228.0
31	12540.0
32	16437.0
33	23678.0
34	38214.0
35	48525.0
36	48746.0
37	75816.0
38	126560.0
39	215889.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.658594306527135	20.115675678897908	24.567147630302795	13.658582384272162
2	34.65874035415059	25.61466675807062	24.057322201921274	15.669270685857523
3	28.81698444443782	24.94657339489191	30.128730548095866	16.107711612574402
4	26.55220308369125	24.44986244698322	31.726759799348446	17.27117466997708
5	22.35705961425544	35.13980334240418	26.024941357408338	16.478195685932036
6	23.866715150503566	33.03001129633659	28.970334449057695	14.13293910410215
7	75.99662600184199	5.197507056484663	14.538593829636937	4.267273112036411
8	77.15695946731364	5.997490365327699	12.417624669529994	4.427925497828659
9	71.74947169507641	6.902389517953425	13.990319128960053	7.357819658010116
10	37.29609218287547	26.217932859821108	22.302515297743415	14.183459659560011
11	27.5924198302867	24.11887084323129	30.244227393169144	18.044481933312866
12	25.13524307987613	21.239050153946117	33.72373750771221	19.901969258465545
13	22.970459632734936	23.537362856810738	35.52712760091444	17.96504990953989
14	19.42314169301982	26.450565860026764	34.24533616288185	19.88095628407157
15	18.21258572846468	25.20319993323537	37.65867776231197	18.925536575987984
16	21.997007514001197	24.805294673434535	33.315549302995166	19.8821485095691
17	21.91504201104597	25.014679276438343	31.61469060258057	21.455588109935114
18	22.313990468157147	24.26268304387092	33.35384954710334	20.069476940868597
19	21.628758863451434	26.428211631948066	31.35672281055239	20.586306694048112
20	22.42695383404817	26.773062857108794	32.296345530793694	18.503637778049338
21	22.113249500010433	26.67470425356252	33.50794469265917	17.704101553767877
22	20.797181578923837	23.463891960525412	34.0158327546072	21.72309370594354
23	19.856515661372192	25.59454795279979	33.197966063301216	21.350970322526802
24	20.99866768800651	25.486055432524505	32.47443421448733	21.040842664981653
25	20.1295353003067	27.58914121016849	30.755841159796965	21.525482329727843
26	19.135070207178988	27.431767444494454	31.81647476803763	21.616687580288936
27	20.5882440604816	26.40049238913048	31.829142163948887	21.182121386439032
28	18.64640678137863	26.75771295382809	32.673684900762126	21.922195364031154
29	20.025066541085582	26.586479566745254	31.51856742184217	21.869886470326996
30	20.18020488395175	26.48707776588864	31.498895701132913	21.833821649026696
31	21.831735254406016	26.013019102433034	29.577773340049536	22.57747230311141
32	21.946039873981764	27.87438116045269	29.795801577910446	20.3837773876551
33	21.692990012130895	25.3693663619537	30.498171424143163	22.439472201772244
34	19.55473358230976	26.863671994921116	30.854348791530427	22.727245631238695
35	19.244009812015843	26.940272483137463	30.878938442417	22.936779262429695
36	22.513539210806332	27.103309319924772	29.019811807205215	21.363339662063684
37	20.99643226519864	27.41999421770634	29.712494821270496	21.87107869582453
38	21.20119699439952	27.25263556349048	29.697293946176977	21.84887349593302
39	21.194341697788722	26.26770827434301	29.735743218472344	22.802206809395926
40	21.35544116814254	24.56073941825357	31.789649694343186	22.2941697192607
41	19.970075140011982	25.445519765608466	30.005484237288645	24.57892085709091
42	21.209840629256618	25.056109112477532	31.66416796072809	22.069882297537756
43	22.34349804922103	25.447457132041958	29.936931271180633	22.272113547556387
44	21.565272855707928	25.297832832101864	29.56585108507423	23.571043227115975
45	20.652922293722632	25.66503828534129	28.598509122015336	25.083530298920735
46	21.234281251955995	26.2128659014566	29.657652448384088	22.895200398203315
47	19.147588574903057	25.982617352246002	32.01125460869669	22.85853946415425
48	20.236835595084454	25.594995037361368	30.874616624988448	23.29355274256573
49	21.580473730801444	24.05598094823655	31.864908928874808	22.4986363920872
50	20.214928451567328	24.123043632472648	31.39442694191179	24.267600974048232
51	19.370236686566898	24.466404575761462	29.411159826769634	26.75219891090201
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	277.0
1	673.0
2	1069.0
3	6909.5
4	12750.0
5	8358.5
6	3967.0
7	3829.5
8	3692.0
9	3681.0
10	3670.0
11	3589.0
12	3508.0
13	3507.5
14	3507.0
15	3309.0
16	3111.0
17	3071.5
18	3032.0
19	2938.5
20	2845.0
21	3141.5
22	3438.0
23	3550.5
24	3663.0
25	4510.5
26	5841.0
27	6324.0
28	7558.5
29	8793.0
30	10466.0
31	12139.0
32	13959.5
33	15780.0
34	17447.5
35	19115.0
36	21049.0
37	22983.0
38	24815.5
39	26648.0
40	29352.5
41	32057.0
42	35939.0
43	39821.0
44	43388.0
45	46955.0
46	56665.5
47	66376.0
48	65651.5
49	64927.0
50	62596.5
51	60266.0
52	53027.5
53	45789.0
54	40255.0
55	34721.0
56	31626.5
57	28532.0
58	26812.0
59	25092.0
60	21519.0
61	17946.0
62	15904.5
63	13863.0
64	12212.0
65	10561.0
66	8795.0
67	7029.0
68	5845.5
69	4662.0
70	3683.5
71	2705.0
72	2210.5
73	1716.0
74	1508.5
75	941.5
76	582.0
77	422.5
78	263.0
79	182.0
80	101.0
81	74.0
82	47.0
83	34.5
84	22.0
85	13.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	671014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.599225229624764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.98515762300829	28.193838303464027
2	10.126955272625686	7.615313443715784
3	3.776291098435876	4.259568586281527
4	2.0558039133105694	3.0918653745803226
5	1.3086954229890915	2.4602966982972947
6	0.9138182053141393	2.0615313912322684
7	0.6872572775770418	1.8088238819222553
8	0.5585180400270023	1.679987646542707
9	0.4749935515037084	1.607345057304657
>10	4.960728338488725	38.688542114728776
>50	0.11474179892624983	2.7523099719354924
>100	0.031805621366247615	2.1613398793638305
>500	0.003220822416835202	0.9465512162531189
>1k	0.001610411208417601	1.5674936581322636
>5k	4.0260280210440027E-4	1.1051927762456453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7301	1.0880547946838666	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	3138	0.46765045140637906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	2690	0.40088582354466523	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	2329	0.3470866479685968	No Hit
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2198	0.32756395544653316	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	975	0.14530248251154224	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	948	0.14127872145737647	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.12667395911262655	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	797	0.11877546519148631	No Hit
GAACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT	786	0.11713615513238174	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	751	0.11192016858068536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4902818719132537E-4	0.0	0.0	0.1463456798218815	0.0
2	1.4902818719132537E-4	0.0	0.0	0.834110763709848	0.0
3	2.9805637438265074E-4	0.0	0.0	1.162866944653912	0.0
4	2.9805637438265074E-4	0.0	0.0	1.6433338201587449	0.0
5	2.9805637438265074E-4	0.0	0.0	3.084436390298861	0.0
6	2.9805637438265074E-4	0.0	0.0	3.962063384668576	0.0
7	2.9805637438265074E-4	0.0	0.0	4.790958161826728	0.0
8	4.470845615739761E-4	0.0	0.0	5.909861791259199	0.0
9	4.470845615739761E-4	0.0	0.0	6.349345915286417	0.0
10	4.470845615739761E-4	0.0	0.0	7.730241097801238	0.0
11	5.961127487653015E-4	0.0	0.0	9.148095270739507	0.0
12	5.961127487653015E-4	0.0	0.0	10.559243175254167	0.0
13	5.961127487653015E-4	0.0	0.0	11.031513500463477	0.0
14	5.961127487653015E-4	0.0	0.0	11.251181048383492	0.0
15	5.961127487653015E-4	0.0	0.0	11.629712643849457	0.0
16	5.961127487653015E-4	0.0	0.0	12.311665628436963	0.0
17	5.961127487653015E-4	0.0	0.0	13.12208091038339	0.0
18	5.961127487653015E-4	0.0	0.0	14.038008148861275	0.0
19	5.961127487653015E-4	0.0	0.0	14.703419004670543	0.0
20	5.961127487653015E-4	0.0	0.0	15.28000906091378	0.0
21	5.961127487653015E-4	0.0	0.0	15.998473951363161	0.0
22	5.961127487653015E-4	0.0	0.0	16.77655011668907	0.0
23	5.961127487653015E-4	0.0	0.0	17.530334687502794	0.0
24	5.961127487653015E-4	0.0	0.0	18.112587814859303	0.0
25	5.961127487653015E-4	0.0	0.0	18.66518433296474	0.0
26	5.961127487653015E-4	0.0	0.0	19.194383425681135	0.0
27	5.961127487653015E-4	0.0	0.0	19.718515560033026	0.0
28	5.961127487653015E-4	0.0	0.0	20.24652242725189	0.0
29	5.961127487653015E-4	0.0	0.0	20.789730169564272	0.0
30	5.961127487653015E-4	0.0	0.0	21.45782353274298	0.0
31	7.451409359566268E-4	0.0	0.0	22.022640362198107	0.0
32	7.451409359566268E-4	0.0	0.0	22.56957380919027	0.0
33	7.451409359566268E-4	0.0	0.0	23.103988888458364	0.0
34	7.451409359566268E-4	0.0	0.0	23.638403967726457	0.0
35	7.451409359566268E-4	0.0	0.0	24.19651452875797	0.0
36	7.451409359566268E-4	0.0	0.0	24.738828101947202	0.0
37	7.451409359566268E-4	0.0	0.0	25.275180547648784	0.0
38	7.451409359566268E-4	0.0	0.0	25.84953518108415	0.0
39	7.451409359566268E-4	0.0	0.0	26.565317564164086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	7.0311845E-4	45.000004	1
CGTGAAG	20	7.0311845E-4	45.000004	43
CGTCTAG	20	7.0311845E-4	45.000004	33
TATTGCG	20	7.0311845E-4	45.000004	1
CGTAGTC	20	7.0311845E-4	45.000004	3
GTAGACG	40	6.8084773E-9	45.000004	1
CACGTCT	20	7.0311845E-4	45.000004	40
CGTTATT	385	0.0	43.246754	1
CGTTTTT	3515	0.0	42.63158	1
ACGGGTC	75	0.0	42.0	5
CCCGTCG	40	3.4553523E-7	39.375004	40
CGCACGG	40	3.4553523E-7	39.375004	2
GCGCATT	35	6.2444014E-6	38.57143	21
CCGTCGA	35	6.2444014E-6	38.57143	41
TTGAGCG	70	0.0	38.57143	1
CGTCGAA	35	6.2444014E-6	38.57143	42
CACGGGA	235	0.0	38.29787	4
GCGATAA	65	9.094947E-12	38.07692	9
CGATGAA	315	0.0	37.857143	19
GTTTGCG	60	1.546141E-10	37.499996	1
>>END_MODULE