##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936028.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707733
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02600980878382	33.0	31.0	34.0	30.0	34.0
2	32.14725468502952	33.0	31.0	34.0	30.0	34.0
3	32.07546348693646	33.0	31.0	34.0	30.0	34.0
4	35.72898395298792	37.0	35.0	37.0	33.0	37.0
5	31.848086778488497	37.0	35.0	37.0	0.0	37.0
6	33.73488872215935	37.0	35.0	37.0	17.0	37.0
7	35.44891081806274	37.0	35.0	37.0	32.0	37.0
8	35.68865377197333	37.0	35.0	37.0	33.0	37.0
9	37.714628256701324	39.0	37.0	39.0	35.0	39.0
10	37.32357824207717	39.0	37.0	39.0	34.0	39.0
11	37.23321082950774	39.0	37.0	39.0	34.0	39.0
12	37.265813520070424	39.0	37.0	39.0	34.0	39.0
13	37.214315285566734	39.0	37.0	39.0	34.0	39.0
14	38.491849327359326	40.0	38.0	41.0	34.0	41.0
15	38.58493104037822	40.0	38.0	41.0	34.0	41.0
16	38.59330849345728	40.0	38.0	41.0	34.0	41.0
17	38.521344913971795	40.0	38.0	41.0	34.0	41.0
18	38.39500206998967	40.0	38.0	41.0	34.0	41.0
19	38.225987766572985	40.0	37.0	41.0	34.0	41.0
20	38.1405219199896	40.0	37.0	41.0	34.0	41.0
21	38.16007449136892	40.0	37.0	41.0	34.0	41.0
22	38.20598446024136	40.0	37.0	41.0	34.0	41.0
23	38.16688073044496	40.0	37.0	41.0	34.0	41.0
24	38.141420563969746	40.0	36.0	41.0	34.0	41.0
25	37.970962213151005	40.0	36.0	41.0	34.0	41.0
26	37.9617157882987	40.0	36.0	41.0	34.0	41.0
27	37.92623630662976	40.0	36.0	41.0	34.0	41.0
28	37.85987653536009	40.0	36.0	41.0	33.0	41.0
29	37.83719283967259	40.0	36.0	41.0	33.0	41.0
30	37.678316540277194	40.0	36.0	41.0	33.0	41.0
31	37.64180135729152	40.0	36.0	41.0	33.0	41.0
32	37.4299107149165	40.0	36.0	41.0	33.0	41.0
33	37.22258817944055	40.0	36.0	41.0	32.0	41.0
34	36.992081759646645	40.0	36.0	41.0	31.0	41.0
35	36.92548602368407	40.0	36.0	41.0	31.0	41.0
36	36.790156739900496	40.0	35.0	41.0	30.0	41.0
37	36.748003837605424	40.0	36.0	41.0	30.0	41.0
38	36.641182479833496	40.0	35.0	41.0	30.0	41.0
39	36.5135623179928	40.0	35.0	41.0	30.0	41.0
40	36.524107255137174	40.0	35.0	41.0	30.0	41.0
41	36.407446028375105	40.0	35.0	41.0	29.0	41.0
42	36.36119553560453	40.0	35.0	41.0	30.0	41.0
43	36.26292118637961	40.0	35.0	41.0	29.0	41.0
44	36.29423666834809	40.0	35.0	41.0	29.0	41.0
45	36.31442931161893	40.0	35.0	41.0	29.0	41.0
46	36.19588743212483	39.0	35.0	41.0	29.0	41.0
47	36.07630278650282	39.0	35.0	41.0	28.0	41.0
48	36.08174410406184	39.0	35.0	41.0	28.0	41.0
49	36.06918569573554	39.0	35.0	41.0	28.0	41.0
50	35.952720870723844	39.0	35.0	41.0	28.0	41.0
51	35.069674580668135	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	6.0
9	27.0
10	20.0
11	12.0
12	11.0
13	17.0
14	16.0
15	20.0
16	41.0
17	81.0
18	170.0
19	347.0
20	765.0
21	1417.0
22	2508.0
23	3730.0
24	5233.0
25	6776.0
26	8810.0
27	9629.0
28	9305.0
29	9096.0
30	10081.0
31	12384.0
32	16049.0
33	22068.0
34	35719.0
35	49132.0
36	48836.0
37	76033.0
38	132835.0
39	246332.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35245919011831	19.941277289599327	24.53891509933831	14.167348420944057
2	32.1978768829488	28.241158742067984	23.77563290110819	15.785331473875036
3	28.241723926961154	27.12039709890594	29.29904356586453	15.338835408268373
4	25.213604565563568	23.673899620337046	33.89738785671998	17.21510795737941
5	24.114178652118806	35.40191004234648	24.445094407071593	16.03881689846312
6	23.132169900230735	35.32885989490387	27.3849036289109	14.154066575954491
7	74.9901445884253	5.106869398487848	15.824187935280678	4.078798077806178
8	76.24245301547336	8.199278541483865	11.658633976372446	3.8996344666703404
9	71.17401618972127	7.008857860238253	12.690520295083033	9.126605654957448
10	35.55804236908552	33.647152245267634	19.485173080808725	11.309632304838125
11	25.214876231573207	23.979523351320342	32.99775480301187	17.807845614094582
12	23.39554606044935	21.2663532716434	34.721286134742904	20.616814533164344
13	22.464262652723555	23.64097760030972	36.59784127630053	17.2969184706662
14	17.807563021647994	28.789105495999195	32.97698425818776	20.426347224165045
15	16.397144120734797	26.303422335824383	38.081734213326214	19.217699330114606
16	19.08911976691775	26.031144513538294	33.27709743646262	21.602638283081333
17	18.870110620813215	26.68209621425029	33.424186804910896	21.023606360025603
18	19.577580810842505	25.4807957238111	33.77219940288216	21.169424062464234
19	19.69951945154458	27.63966071950863	31.465679853843188	21.195139975103604
20	22.206679637659967	26.691139172541057	34.183512708888806	16.918668480910174
21	21.009900626366157	29.057285727809784	32.448960271740894	17.483853374083164
22	20.047673345739142	23.668106475182025	33.83309807512155	22.45112210395728
23	19.63155596814053	27.54470965745557	32.93968205523834	19.88405231916556
24	19.803513471888408	26.209884236004257	32.890228377085705	21.096373915021626
25	18.444808988700544	30.68148581456566	30.8229233340822	20.050781862651593
26	17.670081796383663	28.491253057297033	32.368986609356924	21.469678536962387
27	20.362905219906377	28.21770356900131	30.874637751807533	20.544753459284788
28	16.427098920072964	28.559640429370965	34.36069817289854	20.652562477657536
29	19.004907217834972	25.20413771860292	34.055498330585124	21.735456732976985
30	18.00664940026818	28.344870169965226	31.948771641282804	21.699708788483793
31	19.939581734919805	27.791695455772164	29.340302063066154	22.928420746241873
32	21.968736797634136	27.715112902747226	30.220718830406383	20.09543146921226
33	19.71689888700965	25.953572886950305	29.473544401631692	24.855983824408355
34	18.912499487801192	26.96101495903116	29.97048321895404	24.156002334213607
35	20.69749467666479	25.024126330127324	29.883586041628696	24.3947929515792
36	19.345289819748405	29.705411504055906	29.596189523450228	21.353109152745457
37	18.58356187997451	26.80940411143751	32.462666005400344	22.144368003187644
38	18.836482119669423	27.946556116501565	29.232069156023528	23.984892607805484
39	19.564016373406357	26.071131344730286	31.73866415724574	22.626188124617617
40	21.253919203993597	24.440714224149502	30.47392166254788	23.83144490930902
41	18.750856608353715	25.90072809943863	30.414859841211307	24.93355545099635
42	20.597598246796462	25.778789458736558	31.65233216481357	21.971280129653415
43	20.285616185764972	26.350897866850914	30.529451078302127	22.834034869081986
44	19.532648611835253	25.53208625286655	30.810347970209108	24.124917165089094
45	18.78716973774008	25.934074008135838	29.854479019630286	25.424277234493797
46	21.829560017690287	27.13424412878868	28.498883053354866	22.537312800166166
47	18.092133615360595	25.885326811099667	33.47505344529646	22.54748612824328
48	19.88136769092299	25.422581679814282	31.029215820090343	23.666834809172386
49	19.396015163910686	24.057660162801508	33.90092026230231	22.6454044109855
50	19.306433358342765	24.442551075052315	31.738946749692325	24.512068816912596
51	18.678654238250868	24.427008490490056	29.459556075525654	27.43478119573342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	295.0
1	626.5
2	958.0
3	6568.5
4	12179.0
5	8052.0
6	3925.0
7	3819.0
8	3713.0
9	3718.5
10	3724.0
11	3660.5
12	3597.0
13	3429.0
14	3261.0
15	3269.5
16	3278.0
17	3198.0
18	3118.0
19	3047.5
20	2977.0
21	3533.5
22	4090.0
23	4131.0
24	4172.0
25	4852.5
26	7123.0
27	8713.0
28	10411.0
29	12109.0
30	13155.5
31	14202.0
32	16462.5
33	18723.0
34	21322.5
35	23922.0
36	26595.0
37	29268.0
38	30676.5
39	32085.0
40	34511.5
41	36938.0
42	41343.5
43	45749.0
44	50471.0
45	55193.0
46	70189.5
47	85186.0
48	74892.5
49	64599.0
50	62308.5
51	60018.0
52	51875.5
53	43733.0
54	37946.0
55	32159.0
56	28862.0
57	25565.0
58	22840.0
59	20115.0
60	18142.0
61	16169.0
62	14035.5
63	11902.0
64	9434.0
65	6966.0
66	5410.0
67	3854.0
68	3077.0
69	2300.0
70	1852.0
71	1404.0
72	1142.5
73	881.0
74	700.5
75	426.5
76	333.0
77	242.5
78	152.0
79	123.5
80	95.0
81	62.5
82	30.0
83	23.0
84	16.0
85	10.5
86	5.0
87	5.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	707733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.58037841553422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88915196498753	24.073365318222077
2	10.177953556102038	6.632031567070731
3	3.8004624677638708	3.7146151606134588
4	2.0660425848923305	2.6924979695360247
5	1.3139213557747806	2.140402748969706
6	0.8994940259679054	1.7583513449128034
7	0.7070722553182721	1.6125677151776177
8	0.5718707419406307	1.4905412141758452
9	0.45432140312056823	1.3321766912350124
>10	5.845009251419784	42.179686028720184
>50	0.23395535839866688	4.728000658017989
>100	0.032420779991528856	1.7427213723602026
>500	0.00481930513387591	1.2083001406363434
>1k	0.0017524745941366947	0.9804859617284162
>5k	0.0017524745941366947	3.714256108623543
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6937	0.9801719009852586	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	6499	0.9182841551828161	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	6472	0.9144691571538984	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	6113	0.8637438129916226	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	2916	0.41201978712311005	No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	1532	0.2164658140852553	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	1408	0.19894508239689263	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC	1013	0.1431330741960598	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTA	953	0.13465530079846494	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACATCCGTT	942	0.13310104234223924	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	824	0.11642808799363603	Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	813	0.11487382953741028	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	806	0.11388475597435756	No Hit
TCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	735	0.10385272412053698	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	709	0.10017902231491255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.825924465864952E-4	0.0	0.0	0.12165604825548618	0.0
2	2.825924465864952E-4	0.0	0.0	0.8222027233434077	0.0
3	2.825924465864952E-4	0.0	0.0	1.0642431538447408	0.0
4	2.825924465864952E-4	0.0	0.0	1.5729095577004322	0.0
5	2.825924465864952E-4	0.0	0.0	3.3057664401688207	0.0
6	2.825924465864952E-4	0.0	0.0	4.024540328061571	0.0
7	2.825924465864952E-4	0.0	0.0	4.804212888193711	0.0
8	2.825924465864952E-4	0.0	0.0	5.819002363885816	0.0
9	2.825924465864952E-4	0.0	0.0	6.14030997565466	0.0
10	2.825924465864952E-4	0.0	0.0	7.895067772733503	0.0
11	2.825924465864952E-4	0.0	0.0	9.033491443807199	0.0
12	2.825924465864952E-4	0.0	0.0	10.924176207694144	0.0
13	2.825924465864952E-4	0.0	0.0	11.358944686767467	0.0
14	2.825924465864952E-4	0.0	0.0	11.545596997737848	0.0
15	2.825924465864952E-4	0.0	0.0	11.94984549257983	0.0
16	4.238886698797428E-4	0.0	0.0	12.507400389694984	0.0
17	4.238886698797428E-4	0.0	0.0	13.246662229965255	0.0
18	4.238886698797428E-4	0.0	0.0	13.984511108002595	0.0
19	4.238886698797428E-4	0.0	0.0	14.640690768976436	0.0
20	4.238886698797428E-4	0.0	0.0	15.130988663804006	0.0
21	4.238886698797428E-4	0.0	0.0	15.710585771752907	0.0
22	4.238886698797428E-4	0.0	0.0	16.396296343395036	0.0
23	5.651848931729904E-4	0.0	0.0	17.021390835244365	0.0
24	5.651848931729904E-4	0.0	0.0	17.490353000354652	0.0
25	5.651848931729904E-4	0.0	0.0	17.91254611555488	0.0
26	5.651848931729904E-4	0.0	0.0	18.320185719755898	0.0
27	5.651848931729904E-4	0.0	0.0	18.744780870752106	0.0
28	5.651848931729904E-4	0.0	0.0	19.176864721582856	0.0
29	5.651848931729904E-4	0.0	0.0	19.62689319277185	0.0
30	5.651848931729904E-4	0.0	0.0	20.157177918791408	0.0
31	5.651848931729904E-4	0.0	0.0	20.624161936775593	0.0
32	5.651848931729904E-4	0.0	0.0	21.04564857085935	0.0
33	5.651848931729904E-4	0.0	0.0	21.488894823330266	0.0
34	7.064811164662379E-4	0.0	0.0	21.920413489267844	0.0
35	7.064811164662379E-4	0.0	0.0	22.39531009575645	0.0
36	8.477773397594856E-4	0.0	0.0	22.84477338205227	0.0
37	8.477773397594856E-4	0.0	0.0	23.289150004309533	0.0
38	8.477773397594856E-4	0.0	0.0	23.7335266265668	0.0
39	8.477773397594856E-4	0.0	0.0	24.171827511222453	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.0314476E-4	45.0	1
CAGCGCG	25	3.889281E-5	45.0	1
TCTTGCG	20	7.0314476E-4	45.0	1
TACCCGA	20	7.0314476E-4	45.0	22
CCCTCGA	25	3.889281E-5	45.0	41
GGTCGTT	20	7.0314476E-4	45.0	8
AACCCGC	20	7.0314476E-4	45.0	21
GCGAACC	25	3.889281E-5	45.0	33
GCGCGCA	20	7.0314476E-4	45.0	25
TTTCGCG	20	7.0314476E-4	45.0	1
CGCGCAT	20	7.0314476E-4	45.0	36
CTACGCG	20	7.0314476E-4	45.0	1
TCGCTCG	20	7.0314476E-4	45.0	30
ACTAACG	20	7.0314476E-4	45.0	1
CGTCGTG	20	7.0314476E-4	45.0	33
CGGTCTA	25	3.889281E-5	45.0	31
AATAGCG	20	7.0314476E-4	45.0	1
ATCGGGT	25	3.889281E-5	45.0	4
TACCGTA	20	7.0314476E-4	45.0	23
TGTTGCG	30	2.1643154E-6	44.999996	1
>>END_MODULE