##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936027.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	875317
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97638798286792	33.0	31.0	34.0	30.0	34.0
2	32.10115306797423	33.0	31.0	34.0	30.0	34.0
3	32.03251393495157	33.0	31.0	34.0	30.0	34.0
4	35.70917964577404	37.0	35.0	37.0	33.0	37.0
5	32.02088957486259	37.0	35.0	37.0	0.0	37.0
6	33.798331347386146	37.0	35.0	37.0	17.0	37.0
7	35.41983075845665	37.0	35.0	37.0	32.0	37.0
8	35.60399489556355	37.0	35.0	37.0	33.0	37.0
9	37.54605931336876	39.0	37.0	39.0	35.0	39.0
10	37.246635219012084	39.0	37.0	39.0	34.0	39.0
11	37.222504532643605	39.0	37.0	39.0	34.0	39.0
12	37.16298323921505	39.0	37.0	39.0	34.0	39.0
13	37.0729118707851	39.0	37.0	39.0	33.0	39.0
14	38.30207684758779	40.0	38.0	41.0	33.0	41.0
15	38.36517170350856	40.0	38.0	41.0	34.0	41.0
16	38.39607593591807	40.0	38.0	41.0	34.0	41.0
17	38.27240416900391	40.0	37.0	41.0	34.0	41.0
18	38.16969280843397	40.0	37.0	41.0	34.0	41.0
19	38.022298207392296	40.0	37.0	41.0	34.0	41.0
20	37.90994691066208	40.0	36.0	41.0	33.0	41.0
21	37.9756271156621	40.0	36.0	41.0	34.0	41.0
22	37.99678173735915	40.0	36.0	41.0	34.0	41.0
23	37.961552214797614	40.0	36.0	41.0	34.0	41.0
24	37.920818400647995	40.0	36.0	41.0	33.0	41.0
25	37.783002043830976	40.0	36.0	41.0	33.0	41.0
26	37.7599372570166	40.0	36.0	41.0	33.0	41.0
27	37.6963054527674	40.0	36.0	41.0	33.0	41.0
28	37.631246736896465	40.0	36.0	41.0	33.0	41.0
29	37.59273268998545	40.0	36.0	41.0	33.0	41.0
30	37.4505224964213	40.0	35.0	41.0	33.0	41.0
31	37.419253824614394	40.0	35.0	41.0	33.0	41.0
32	37.16386406296233	40.0	35.0	41.0	32.0	41.0
33	37.04660483002158	40.0	35.0	41.0	32.0	41.0
34	36.82747278985784	40.0	35.0	41.0	31.0	41.0
35	36.71942279197137	40.0	35.0	41.0	31.0	41.0
36	36.61300991526498	40.0	35.0	41.0	30.0	41.0
37	36.53677810438961	40.0	35.0	41.0	30.0	41.0
38	36.387592152328814	39.0	35.0	41.0	30.0	41.0
39	36.24481987668467	39.0	35.0	41.0	30.0	41.0
40	36.228294435044674	39.0	35.0	41.0	30.0	41.0
41	36.14174978893361	39.0	35.0	41.0	29.0	41.0
42	36.138383008670004	39.0	35.0	41.0	29.0	41.0
43	36.09695230413667	39.0	35.0	41.0	29.0	41.0
44	36.13687955335038	39.0	35.0	41.0	29.0	41.0
45	36.11105919341222	39.0	35.0	41.0	29.0	41.0
46	35.97154173859299	39.0	35.0	41.0	29.0	41.0
47	35.846123175946545	39.0	35.0	41.0	28.0	41.0
48	35.823891230262866	39.0	35.0	41.0	28.0	41.0
49	35.78917123739171	39.0	35.0	41.0	28.0	41.0
50	35.66275989155929	39.0	35.0	41.0	28.0	41.0
51	34.773589453877854	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	15.0
9	36.0
10	27.0
11	22.0
12	13.0
13	24.0
14	20.0
15	29.0
16	69.0
17	99.0
18	249.0
19	609.0
20	1214.0
21	2327.0
22	3756.0
23	4990.0
24	6392.0
25	8109.0
26	9863.0
27	10753.0
28	11003.0
29	11886.0
30	13777.0
31	17244.0
32	22304.0
33	31220.0
34	50137.0
35	67130.0
36	64974.0
37	97751.0
38	163643.0
39	275447.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.778843550393745	18.751835049473506	22.26119223092891	13.208129169203842
2	32.106425443582154	28.93694512959305	23.080209798278794	15.876419628546001
3	29.256829240149568	28.961393415185583	26.661426660284214	15.120350684380629
4	26.36987514237699	22.760668420697872	33.52716787175389	17.342288565171245
5	25.77089214535991	34.67406665242421	23.254775127182494	16.300266075033388
6	23.552724327300854	36.850878024761315	25.911412665354376	13.684984982583453
7	72.39902800928121	5.974521230594173	17.196512806217633	4.429937953906984
8	72.473629553636	10.81322538006231	11.960352649383022	4.752792416918671
9	66.54332087689374	7.946492527849911	13.527442058134367	11.98274453712198
10	37.95196483102693	26.74973752366285	21.183982488629834	14.11431515668038
11	30.426691130184835	23.50702659722135	28.079884201951977	17.986398070641833
12	26.363705948816257	21.07476491373982	30.784275868056945	21.777253269386975
13	24.953588242888006	22.36778218633935	33.79438534839378	18.88424422237886
14	19.580791873115682	28.460089316213438	30.570981712910868	21.388137097760012
15	18.262983581948024	24.59314739688593	37.23633837798192	19.907530643184128
16	20.874266123016007	24.972095823570204	30.989915653414705	23.163722399999088
17	20.092606450006112	26.34622656706085	32.11647894419964	21.444688038733396
18	21.112351296730214	25.188017598195856	31.97607266853037	21.72355843654356
19	21.3339852876158	27.140338871517404	28.873311040457345	22.65236480040945
20	24.754346139741372	25.59278524237505	31.724506664442707	17.92836195344087
21	22.63385721972725	29.41460065324905	29.849300310630323	18.102241816393374
22	21.002790988864607	23.521078649220797	32.543295743142195	22.9328346187724
23	22.51732800802452	26.896198748567663	30.82597504675449	19.760498196653327
24	21.767314013094683	24.958158015895958	30.63427306907098	22.640254901938384
25	19.793057829334973	29.79686216536409	28.514698103658446	21.89538190164249
26	19.167227415896186	25.14391928866913	32.05661491779549	23.632238377639187
27	22.30357687557765	26.14378562280865	28.617517996337327	22.935119505276376
28	18.162105842797523	27.624277833059335	32.406888018854886	21.806728305288257
29	21.512434923576258	24.07584909238596	30.890980067792583	23.5207359162452
30	20.906597267047253	26.832221926456356	29.966857721259842	22.29432308523655
31	23.681820414775448	24.159590182756645	28.177563099996917	23.98102630247099
32	23.800748757307353	26.071468964957837	29.494800169538575	20.63298210819623
33	23.906995979742195	24.547678155456822	28.28643794191133	23.258887922889652
34	21.366887653273043	24.246073136932107	31.17533419321229	23.211705016582567
35	20.82971083618849	25.798882005033608	29.180971008217593	24.19043615056031
36	22.1424923770474	27.64301390239193	28.476312010391663	21.738181710169002
37	20.20593682060328	26.21530257038307	31.89564466359045	21.6831159454232
38	23.157210473462758	26.417972003285666	27.729839589542987	22.69497793370859
39	22.328253649820578	24.797530494666503	31.846405359429784	21.027810496083134
40	22.399313620094205	25.110902678686692	30.464506001825626	22.025277699393477
41	19.117416890109524	25.625344875056694	31.39239841108993	23.864839823743857
42	20.16915014788928	25.71582638061411	31.197726080951245	22.917297390545368
43	21.87904496313907	24.48838535067867	29.93635448643177	23.69621519975049
44	21.93593863708805	24.151821568643133	29.85786863502023	24.054371159248593
45	21.043461968635363	23.625726451102857	29.3002420837251	26.030569496536682
46	23.2082776868266	26.311724780850827	28.519382120991594	21.96061541133098
47	19.686696362574928	25.135807941580023	33.15781596838631	22.019679727458737
48	21.017414262490046	24.7335536725552	30.319187220172804	23.929844844781947
49	20.53393227824891	22.79391351933071	33.576407175914554	23.095747026505826
50	20.094777092184888	23.11459734016362	31.667612990493733	25.12301257715776
51	19.71160162546826	22.475057607700982	30.078245938328628	27.73509482850213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	965.0
2	1550.0
3	8089.5
4	14629.0
5	9396.0
6	4163.0
7	3912.0
8	3661.0
9	3680.5
10	3700.0
11	3579.5
12	3459.0
13	3404.5
14	3350.0
15	3308.5
16	3267.0
17	3211.5
18	3156.0
19	3173.0
20	3190.0
21	3666.5
22	4143.0
23	4352.0
24	4561.0
25	5281.0
26	7057.0
27	8113.0
28	9845.0
29	11577.0
30	13419.5
31	15262.0
32	17952.5
33	20643.0
34	21891.5
35	23140.0
36	25211.0
37	27282.0
38	29371.0
39	31460.0
40	35047.0
41	38634.0
42	43355.0
43	48076.0
44	52859.0
45	57642.0
46	81270.5
47	104899.0
48	91578.0
49	78257.0
50	76082.5
51	73908.0
52	65136.5
53	56365.0
54	51570.0
55	46775.0
56	43825.0
57	40875.0
58	39218.5
59	37562.0
60	34325.5
61	31089.0
62	27859.5
63	24630.0
64	20399.5
65	16169.0
66	12784.0
67	9399.0
68	7461.0
69	5523.0
70	4814.5
71	4106.0
72	3030.5
73	1955.0
74	1533.0
75	1019.0
76	927.0
77	689.5
78	452.0
79	285.5
80	119.0
81	120.0
82	121.0
83	70.5
84	20.0
85	12.0
86	4.0
87	4.5
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	875317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.814362065502994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18144754281202	22.55039621061824
2	10.40988234502569	6.415477672778179
3	3.88511811777795	3.5915230904536575
4	2.077364845397444	2.5605068995289795
5	1.2474028371756793	1.921896133313353
6	0.9382327598348151	1.7346626377959669
7	0.6690483296232018	1.4431408227830531
8	0.5569105211123718	1.372867394851566
9	0.4729490167149199	1.3116260015619454
>10	6.274005359225825	43.063425848438335
>50	0.23184478593873628	4.368269658000469
>100	0.048253871880068966	2.6688913760970228
>500	0.002261900244378229	0.5272730339980142
>1k	0.0037698337406303815	2.0973594687891928
>5k	0.0011309501221891144	3.061645314838436
>10k+	3.7698337406303815E-4	1.3110384361536875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	11286	1.2893614541931666	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	9294	1.0617867583972436	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	8789	1.0040933741718714	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8273	0.9451433023693131	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3781	0.4319577935764986	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	2251	0.25716397602240104	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	1937	0.2212912579099915	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA	1783	0.20369763182938294	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	1698	0.19398686418748864	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT	1596	0.18233394301721548	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	1339	0.15297315144113505	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	1333	0.15228768548994248	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1289	0.1472609351811972	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1048	0.11972805280829689	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	944	0.1078466429876262	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	888	0.10144896077649583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2848865039751313E-4	0.0	0.0	0.1601705439286567	0.0
2	2.2848865039751313E-4	0.0	0.0	0.9249220568091332	0.0
3	2.2848865039751313E-4	0.0	0.0	1.1772877711731864	0.0
4	2.2848865039751313E-4	0.0	0.0	1.6934436324211686	0.0
5	2.2848865039751313E-4	0.0	0.0	3.5974395561836454	0.0
6	2.2848865039751313E-4	0.0	0.0	4.306668327017526	0.0
7	2.2848865039751313E-4	0.0	0.0	5.1959461543646475	0.0
8	2.2848865039751313E-4	0.0	0.0	6.26698670310299	0.0
9	2.2848865039751313E-4	0.0	0.0	6.643878731933688	0.0
10	2.2848865039751313E-4	0.0	0.0	8.893692228072801	0.0
11	2.2848865039751313E-4	0.0	0.0	9.972844123900256	0.0
12	2.2848865039751313E-4	0.0	0.0	11.972576792179291	0.0
13	3.427329755962697E-4	0.0	0.0	12.410475290666124	0.0
14	3.427329755962697E-4	0.0	0.0	12.617714496576669	0.0
15	3.427329755962697E-4	0.0	0.0	13.117076442020434	0.0
16	3.427329755962697E-4	0.0	0.0	13.699037034582899	0.0
17	3.427329755962697E-4	0.0	0.0	14.34120438652511	0.0
18	3.427329755962697E-4	0.0	0.0	14.996738324515576	0.0
19	3.427329755962697E-4	0.0	0.0	15.677862991350562	0.0
20	4.5697730079502627E-4	0.0	0.0	16.170141788632005	0.0
21	4.5697730079502627E-4	0.0	0.0	16.721142169065608	0.0
22	4.5697730079502627E-4	0.0	0.0	17.391413625006713	0.0
23	4.5697730079502627E-4	0.0	0.0	17.96960415483762	0.0
24	4.5697730079502627E-4	0.0	0.0	18.42052650639711	0.0
25	4.5697730079502627E-4	0.0	0.0	18.864479954119478	0.0
26	4.5697730079502627E-4	0.0	0.0	19.254624324673234	0.0
27	4.5697730079502627E-4	0.0	0.0	19.68384025444496	0.0
28	5.712216259937829E-4	0.0	0.0	20.08757969969737	0.0
29	5.712216259937829E-4	0.0	0.0	20.493147054152953	0.0
30	5.712216259937829E-4	0.0	0.0	20.97868543624767	0.0
31	5.712216259937829E-4	0.0	0.0	21.39647693349952	0.0
32	5.712216259937829E-4	0.0	0.0	21.81918093673492	0.0
33	5.712216259937829E-4	0.0	0.0	22.254451815742183	0.0
34	9.139546015900525E-4	0.0	0.0	22.667673539986087	0.0
35	9.139546015900525E-4	0.0	0.0	23.11482582881402	0.0
36	9.139546015900525E-4	0.0	0.0	23.53752983204942	0.0
37	9.139546015900525E-4	0.0	0.0	23.982168745722976	0.0
38	9.139546015900525E-4	0.0	0.0	24.426236437770545	0.0
39	9.139546015900525E-4	0.0	0.0	24.868247731964534	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGCGA	30	2.1649066E-6	45.000004	11
CGCATCG	20	7.032365E-4	45.0	21
TAGCACG	50	2.1827873E-11	45.0	1
TCTACGG	20	7.032365E-4	45.0	2
GTCGTTA	20	7.032365E-4	45.0	17
GTTCACG	50	2.1827873E-11	45.0	1
CCAACCG	20	7.032365E-4	45.0	40
TAATACG	385	0.0	42.662334	4
CCAATCG	65	0.0	41.53846	40
CGTTTTT	3400	0.0	41.42647	1
CGATGAA	1325	0.0	41.09434	19
CTTGGCG	55	6.002665E-11	40.909092	1
TACGAAT	110	0.0	40.909092	12
TACGGGT	100	0.0	40.5	4
AACGAGC	100	0.0	40.5	15
GCTAACG	50	1.0804797E-9	40.5	1
CGTTATT	560	0.0	40.17857	1
TACGGCT	1240	0.0	39.737904	7
AATACGG	415	0.0	39.578312	5
GCTACGA	115	0.0	39.130436	10
>>END_MODULE