##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936026.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	937008
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10762554855455	33.0	31.0	34.0	30.0	34.0
2	32.22167046599389	34.0	31.0	34.0	30.0	34.0
3	32.15790580229838	33.0	31.0	34.0	30.0	34.0
4	35.774394668988954	37.0	35.0	37.0	35.0	37.0
5	31.943009024469376	37.0	35.0	37.0	0.0	37.0
6	33.80387147174837	37.0	35.0	37.0	17.0	37.0
7	35.5007406553626	37.0	35.0	37.0	32.0	37.0
8	35.7708461400543	37.0	35.0	37.0	35.0	37.0
9	37.74769799190615	39.0	38.0	39.0	35.0	39.0
10	37.36293500162218	39.0	37.0	39.0	34.0	39.0
11	37.31920752010655	39.0	37.0	39.0	34.0	39.0
12	37.224324658914334	39.0	37.0	39.0	34.0	39.0
13	37.15575534040264	39.0	37.0	39.0	34.0	39.0
14	38.25215579802947	40.0	38.0	41.0	33.0	41.0
15	38.43651921861927	40.0	38.0	41.0	34.0	41.0
16	38.40282687020815	40.0	38.0	41.0	34.0	41.0
17	38.42415646397896	40.0	38.0	41.0	34.0	41.0
18	38.31381375612588	40.0	37.0	41.0	34.0	41.0
19	38.17892376585899	40.0	37.0	41.0	34.0	41.0
20	38.23942805184161	40.0	37.0	41.0	34.0	41.0
21	38.136190939671806	40.0	37.0	41.0	34.0	41.0
22	38.22946122124891	40.0	37.0	41.0	34.0	41.0
23	38.24839595819886	40.0	37.0	41.0	34.0	41.0
24	38.204205300274914	40.0	36.0	41.0	34.0	41.0
25	37.93415531137408	40.0	36.0	41.0	34.0	41.0
26	38.00634786469272	40.0	36.0	41.0	34.0	41.0
27	37.983402489626556	40.0	36.0	41.0	34.0	41.0
28	37.925095623516555	40.0	36.0	41.0	34.0	41.0
29	37.8811824445469	40.0	36.0	41.0	34.0	41.0
30	37.695372931714566	40.0	36.0	41.0	33.0	41.0
31	37.64790802213001	40.0	36.0	41.0	33.0	41.0
32	37.564222503970086	40.0	35.0	41.0	33.0	41.0
33	37.45127789730717	40.0	35.0	41.0	33.0	41.0
34	37.33982100472995	40.0	35.0	41.0	33.0	41.0
35	37.28735293615423	40.0	35.0	41.0	33.0	41.0
36	37.18191306797807	40.0	35.0	41.0	32.0	41.0
37	37.13678218328979	40.0	35.0	41.0	32.0	41.0
38	37.00655490668169	40.0	35.0	41.0	32.0	41.0
39	36.9120701210662	39.0	35.0	41.0	32.0	41.0
40	36.853024734047096	39.0	35.0	41.0	31.0	41.0
41	36.72712933080614	39.0	35.0	41.0	31.0	41.0
42	36.714483761077815	39.0	35.0	41.0	31.0	41.0
43	36.66376914604785	39.0	35.0	41.0	31.0	41.0
44	36.60763728804877	39.0	35.0	41.0	31.0	41.0
45	36.56817124293496	39.0	35.0	41.0	31.0	41.0
46	36.47136097023718	39.0	35.0	41.0	31.0	41.0
47	36.418149044618616	39.0	35.0	41.0	31.0	41.0
48	36.35435556580093	39.0	35.0	41.0	31.0	41.0
49	36.31540178952581	39.0	35.0	41.0	31.0	41.0
50	36.20895766098048	39.0	35.0	41.0	31.0	41.0
51	35.22753914587709	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	24.0
9	57.0
10	45.0
11	39.0
12	22.0
13	18.0
14	40.0
15	38.0
16	65.0
17	147.0
18	275.0
19	465.0
20	799.0
21	1125.0
22	1787.0
23	2745.0
24	4093.0
25	6026.0
26	7742.0
27	9021.0
28	10148.0
29	11246.0
30	13418.0
31	16867.0
32	22415.0
33	34084.0
34	58215.0
35	70351.0
36	71198.0
37	111348.0
38	181398.0
39	301512.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.385123712924546	20.589685466933048	24.672574833939517	12.352615986202894
2	34.11827860594573	23.57471867902942	24.88559329269334	17.421409422331507
3	32.866421631405494	23.21890528149173	27.299233304304764	16.61543978279801
4	28.705197821149874	24.570761402250568	27.367855984153817	19.35618479244574
5	22.126491982992675	37.39818656831105	23.389127947680276	17.086193501016
6	25.324863821867048	33.50110137800318	26.40788552499018	14.766149275139592
7	78.87862216758022	6.314140327510544	10.153488550791455	4.653748954117788
8	80.58309000563496	5.6171345388726674	9.552213001383125	4.24756245410925
9	74.51323788057306	7.290652801256766	12.104805935488278	6.091303382681898
10	40.223669381691515	25.89486962758055	19.142739442993015	14.738721547734917
11	30.15000939159538	26.030087256458856	25.300104161330534	18.519799190615235
12	27.436051773303966	22.854660792650648	30.759929477656538	18.949357956388845
13	24.859019346686477	23.374293495893312	32.11541416935608	19.651272988064136
14	21.17996858084456	26.01696036746751	32.58083175383775	20.222239297850177
15	20.290008196301418	25.068302511824875	34.66053651623039	19.981152775643324
16	24.948239502757712	24.362225295835255	31.264300838413334	19.4252343629937
17	24.793064733705585	24.44152024315694	29.095589365298906	21.66982565783857
18	25.396688181957895	24.113454741048102	31.00261683998429	19.487240237009715
19	24.674709287434045	26.091346071751786	28.418647439509588	20.815297201304578
20	24.751015999863395	26.788992196438024	28.951727199767774	19.508264603930808
21	24.667131977528474	26.316637638099138	30.696002595495447	18.320227788876934
22	23.845047214111297	23.877170739203933	30.783515188771066	21.4942668579137
23	21.99308863958472	25.006296637808855	31.17358656489592	21.827028157710497
24	22.142607106876355	25.597540255792907	30.953631132284894	21.306221505045848
25	23.040358246674522	26.232326725065313	28.983850724860403	21.743464303399758
26	21.37302989942455	25.912265423560953	28.767950753888975	23.94675392312552
27	21.000354319280092	26.21834605467616	30.731968136878233	22.049331489165514
28	20.03462083568123	26.62741406690231	30.171567371890102	23.166397725526355
29	21.239626556016596	26.600733398220722	30.1383766200502	22.02126342571248
30	22.76554735925414	26.201056981370492	29.85171951573519	21.181676143640182
31	24.56745299933405	25.77704779468265	28.457921383808888	21.19757782217441
32	24.50736813346311	26.02432423202363	28.094103785666718	21.37420384884654
33	23.562445571435887	26.023683895975275	28.096131516486516	22.317739016102315
34	21.795118077967317	25.479078086846645	30.095687550159656	22.630116285026382
35	21.53770298652733	27.095926608951043	29.177765824838207	22.188604579683417
36	22.99959018492905	28.226226456977955	27.4859979850759	21.288185373017093
37	21.98817939654731	27.71406434096614	28.75044823523385	21.5473080272527
38	22.89991120673463	27.478100507146152	28.106803783959155	21.515184502160068
39	21.760753376705427	26.037130952990793	29.076912897221796	23.12520277308198
40	23.993071563956764	24.690824411317728	28.76955159400987	22.54655243071564
41	20.994057681471237	26.062210781551492	28.161445793419055	24.78228574355822
42	21.82766849375886	25.60693185116883	29.788112801598277	22.777286853474035
43	22.20002390587914	24.95389580451821	29.86580690879907	22.980273380803578
44	22.0306550210884	24.903095811348464	28.887800317606683	24.178448849956457
45	21.67761641309359	25.03639243208169	28.083538240868812	25.20245291395591
46	22.239831463552072	25.664028482147433	28.747460000341512	23.348680053958983
47	21.018390451308846	25.910878028789508	30.025357307514984	23.045374212386662
48	20.937708109215716	26.30116285026382	30.097822003654183	22.663307036866282
49	22.49020285846012	24.841516828031352	30.678179908816144	21.990100404692384
50	20.834400560080596	25.241940303604665	30.501020268770386	23.422638867544354
51	20.668233355531648	24.895411778768164	29.152579273602786	25.283775592097403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	551.0
1	1072.5
2	1594.0
3	6277.5
4	10961.0
5	6930.0
6	2899.0
7	2761.0
8	2623.0
9	2692.0
10	2761.0
11	2722.5
12	2684.0
13	2637.5
14	2591.0
15	2495.0
16	2399.0
17	2495.0
18	2591.0
19	2656.5
20	2722.0
21	3052.0
22	3382.0
23	4053.5
24	4725.0
25	5575.5
26	7716.5
27	9007.0
28	10864.0
29	12721.0
30	15243.0
31	17765.0
32	19652.0
33	21539.0
34	24806.0
35	28073.0
36	29708.0
37	31343.0
38	35059.0
39	38775.0
40	41246.5
41	43718.0
42	47852.0
43	51986.0
44	55990.0
45	59994.0
46	66876.5
47	73759.0
48	77582.5
49	81406.0
50	80968.5
51	80531.0
52	72502.0
53	64473.0
54	60284.5
55	56096.0
56	52523.0
57	48950.0
58	46382.0
59	43814.0
60	39762.0
61	35710.0
62	31581.0
63	27452.0
64	24435.5
65	21419.0
66	17910.5
67	14402.0
68	12221.0
69	10040.0
70	8103.5
71	6167.0
72	5078.5
73	3990.0
74	3192.5
75	1828.0
76	1261.0
77	964.5
78	668.0
79	501.5
80	335.0
81	214.5
82	94.0
83	113.0
84	132.0
85	76.5
86	21.0
87	30.5
88	40.0
89	28.0
90	16.0
91	9.5
92	3.0
93	1.5
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	937008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.350592252719107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48291530404364	21.99899407681402
2	10.014828204001859	6.079119346013832
3	3.964820348868562	3.610039372913798
4	2.1716445767824193	2.6364279627100786
5	1.3947010156692727	2.116500092051565
6	1.0001810409513523	1.8213652171688792
7	0.7202706134063132	1.5302447789377622
8	0.5861896772397623	1.4232963101325635
9	0.5060841833938201	1.382395922616254
>10	6.8062979102877454	48.158924046806675
>50	0.31207573860263726	5.675207136042882
>100	0.0360636612680258	1.931290845201487
>500	0.002856527625190046	0.6314814968289543
>1k	7.141319062975115E-4	0.2917364320342449
>5k	3.570659531487558E-4	0.7129769637270791
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6579	0.7021284770247426	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1683	0.17961426156446902	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1009	0.10768317879890034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.403360483581784E-4	0.0	0.0	0.10352099448457217	0.0
2	6.403360483581784E-4	0.0	0.0	0.476196574629032	0.0
3	6.403360483581784E-4	0.0	0.0	0.6718192374024555	0.0
4	6.403360483581784E-4	0.0	0.0	0.928593992794085	0.0
5	6.403360483581784E-4	0.0	0.0	1.6579367518740502	0.0
6	6.403360483581784E-4	0.0	0.0	2.2731929716715333	0.0
7	6.403360483581784E-4	0.0	0.0	2.815130713932005	0.0
8	6.403360483581784E-4	0.0	0.0	3.60669279237744	0.0
9	6.403360483581784E-4	0.0	0.0	3.965601147482199	0.0
10	6.403360483581784E-4	0.0	0.0	4.706469955432611	0.0
11	6.403360483581784E-4	0.0	0.0	5.673163943104008	0.0
12	6.403360483581784E-4	0.0	0.0	6.465152912248348	0.0
13	6.403360483581784E-4	0.0	0.0	6.816804125471714	0.0
14	6.403360483581784E-4	0.0	0.0	7.011893174871506	0.0
15	6.403360483581784E-4	0.0	0.0	7.268667930263136	0.0
16	6.403360483581784E-4	0.0	0.0	7.735899800215153	0.0
17	6.403360483581784E-4	0.0	0.0	8.271861072690948	0.0
18	6.403360483581784E-4	0.0	0.0	8.834716459197788	0.0
19	6.403360483581784E-4	0.0	0.0	9.236954220241449	0.0
20	6.403360483581784E-4	0.0	0.0	9.643567610948892	0.0
21	6.403360483581784E-4	0.0	0.0	10.140788552499018	0.0
22	6.403360483581784E-4	0.0	0.0	10.642705291737103	0.0
23	6.403360483581784E-4	0.0	0.0	11.116767412871608	0.0
24	6.403360483581784E-4	0.0	0.0	11.523487526253778	0.0
25	6.403360483581784E-4	0.0	0.0	11.89402865290371	0.0
26	6.403360483581784E-4	0.0	0.0	12.247814319621604	0.0
27	7.470587230845415E-4	0.0	0.0	12.62529242012875	0.0
28	8.537813978109045E-4	0.0	0.0	12.986015060703858	0.0
29	8.537813978109045E-4	0.0	0.0	13.400312483991598	0.0
30	8.537813978109045E-4	0.0	0.0	13.917383843040827	0.0
31	9.605040725372676E-4	0.0	0.0	14.321862780253744	0.0
32	9.605040725372676E-4	0.0	0.0	14.725381213394122	0.0
33	9.605040725372676E-4	0.0	0.0	15.10840889298704	0.0
34	9.605040725372676E-4	0.0	0.0	15.529963458156173	0.0
35	0.0010672267472636306	0.0	0.0	15.977344910609087	0.0
36	0.0010672267472636306	0.0	0.0	16.380329730375834	0.0
37	0.0010672267472636306	0.0	0.0	16.775417072212832	0.0
38	0.0010672267472636306	0.0	0.0	17.22994894387241	0.0
39	0.0010672267472636306	0.0	0.0	17.910305995252976	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	25	3.8902526E-5	45.0	12
TATCTCG	25	3.8902526E-5	45.0	23
TATCGAC	20	7.032618E-4	45.0	30
CGTTTTT	2545	0.0	41.905697	1
TAGCACG	50	1.0804797E-9	40.5	1
TTGCACG	45	1.927583E-8	40.000004	1
TCGAAAG	45	1.927583E-8	40.000004	1
ACAACGA	220	0.0	39.886364	13
CCTCACG	40	3.4573168E-7	39.375	42
CGTTATT	350	0.0	39.214287	1
CACAACG	230	0.0	39.130436	12
CGTAAGG	145	0.0	38.793102	2
TGCACGG	210	0.0	38.57143	2
ATATCGT	35	6.2470535E-6	38.571426	33
ACGTAAG	35	6.2470535E-6	38.571426	1
GCGTAAG	95	0.0	37.894737	1
CGACAAT	30	1.13976086E-4	37.500004	20
AAAGTCG	30	1.13976086E-4	37.500004	33
GCCGACC	30	1.13976086E-4	37.500004	14
TATTCGC	30	1.13976086E-4	37.500004	26
>>END_MODULE