SRR2936025.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936025.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	696671
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15660	2.2478329082163606	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	2205	0.31650520834080936	RNA PCR Primer, Index 4 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1858	0.2666969057130267	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG	1041	0.1494249078833481	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	839	0.12042987292423539	RNA PCR Primer, Index 48 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	836	0.11999925359315947	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT	741	0.10636297477575499	RNA PCR Primer, Index 4 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	730	0.10478403722847657	TruSeq Adapter, Index 4 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	20	7.031371E-4	45.0	1
ATTCGCG	20	7.031371E-4	45.0	1
GGACGTA	20	7.031371E-4	45.0	8
CCAACGG	20	7.031371E-4	45.0	2
TAGTGCG	25	3.889218E-5	45.0	1
CCGTTAC	20	7.031371E-4	45.0	23
CGAATAT	55	1.8189894E-12	44.999996	14
CGTTTTT	8265	0.0	44.07441	1
CGTTATT	1095	0.0	43.767128	1
GCGATCA	70	0.0	41.785713	9
AGTACGG	115	0.0	41.086956	2
TACGGGT	55	6.002665E-11	40.90909	4
CTAACGG	85	0.0	39.705883	2
CGTAAGG	120	0.0	39.375004	2
GGCGATA	180	0.0	38.75	8
TATAGCG	35	6.2447452E-6	38.571426	1
CACAACG	140	0.0	38.571426	12
ACGGGTA	65	9.094947E-12	38.07692	5
TAGCGCG	30	1.1394603E-4	37.500004	1
AAGCGCG	30	1.1394603E-4	37.500004	1