##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936024.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394832
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.839379786846052	33.0	31.0	34.0	30.0	34.0
2	31.998308141184097	33.0	31.0	34.0	30.0	34.0
3	31.87327774851076	33.0	31.0	34.0	30.0	34.0
4	35.616745856465535	37.0	35.0	37.0	33.0	37.0
5	32.284840134538236	37.0	35.0	37.0	19.0	37.0
6	33.88395570774405	37.0	35.0	37.0	19.0	37.0
7	35.301363617943835	37.0	35.0	37.0	32.0	37.0
8	35.416701280544636	37.0	35.0	37.0	33.0	37.0
9	37.31323195688293	39.0	37.0	39.0	34.0	39.0
10	37.057079973254446	39.0	37.0	39.0	33.0	39.0
11	37.00644831219354	39.0	37.0	39.0	33.0	39.0
12	36.92709810754954	39.0	35.0	39.0	33.0	39.0
13	36.73357276005998	39.0	35.0	39.0	32.0	39.0
14	37.977706974105445	40.0	37.0	41.0	33.0	41.0
15	38.02902247031649	40.0	37.0	41.0	33.0	41.0
16	38.19404455565912	40.0	37.0	41.0	34.0	41.0
17	37.85442416014913	40.0	36.0	41.0	32.0	41.0
18	37.77800938120517	39.0	36.0	41.0	33.0	41.0
19	37.58749290837622	39.0	36.0	41.0	33.0	41.0
20	36.98268630708757	39.0	35.0	41.0	31.0	41.0
21	37.30025428536694	39.0	35.0	41.0	32.0	41.0
22	37.193586132836245	39.0	35.0	41.0	32.0	41.0
23	36.97682811930137	39.0	35.0	41.0	31.0	41.0
24	37.115763160027555	39.0	35.0	41.0	32.0	41.0
25	37.20145276978563	39.0	35.0	41.0	33.0	41.0
26	36.93261944320622	39.0	35.0	41.0	31.0	41.0
27	36.697848705272115	39.0	35.0	41.0	31.0	41.0
28	36.65587135794465	39.0	35.0	41.0	30.0	41.0
29	36.635455079628805	39.0	35.0	41.0	30.0	41.0
30	36.44627841714957	39.0	35.0	41.0	30.0	41.0
31	36.43226992746282	39.0	35.0	41.0	30.0	41.0
32	35.7439088017182	39.0	35.0	41.0	26.0	41.0
33	35.42479079709851	39.0	35.0	41.0	23.0	41.0
34	35.09973102484094	39.0	35.0	41.0	21.0	41.0
35	34.69025306966001	39.0	35.0	41.0	18.0	41.0
36	34.46359970823033	39.0	35.0	41.0	16.0	41.0
37	34.315524070997284	39.0	35.0	41.0	13.0	41.0
38	34.25653949021356	39.0	34.0	41.0	15.0	41.0
39	34.091897313287674	39.0	34.0	41.0	12.0	41.0
40	34.0648807594116	38.0	34.0	41.0	15.0	41.0
41	34.16879330955951	38.0	33.0	41.0	18.0	41.0
42	33.876861551242044	38.0	34.0	41.0	13.0	41.0
43	33.72760313247153	38.0	34.0	41.0	11.0	41.0
44	33.90221157353001	38.0	34.0	41.0	12.0	41.0
45	33.95251651335251	38.0	34.0	41.0	15.0	41.0
46	33.809620294201075	38.0	34.0	40.0	13.0	41.0
47	33.5573535073145	38.0	33.0	40.0	11.0	41.0
48	33.56355614539855	38.0	33.0	40.0	11.0	41.0
49	33.59513160027556	38.0	34.0	40.0	11.0	41.0
50	33.42057887911821	38.0	33.0	40.0	10.0	41.0
51	32.67653837581554	37.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	12.0
10	11.0
11	11.0
12	5.0
13	5.0
14	11.0
15	21.0
16	36.0
17	71.0
18	184.0
19	546.0
20	1294.0
21	2835.0
22	4586.0
23	5983.0
24	7012.0
25	8221.0
26	9208.0
27	8794.0
28	7740.0
29	6997.0
30	7522.0
31	8757.0
32	10775.0
33	14763.0
34	22455.0
35	29652.0
36	26279.0
37	37229.0
38	61965.0
39	111758.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.412722373059935	18.243708716618716	21.428607610325404	17.914961299995948
2	32.09213032378328	36.08977995704502	18.68237630181951	13.135713417352191
3	23.903584309275843	34.82747092434251	28.530109008388376	12.738835757993272
4	22.65875106374357	19.730163715200387	42.989170077400004	14.621915143656036
5	29.692882035903878	29.998834947521985	24.849049722413582	15.459233294160555
6	19.428769704583214	39.36383069254772	29.084268752279453	12.123130850589618
7	63.813470032824085	4.826609798597885	27.764213640231794	3.5957065283462333
8	63.0997487539004	16.358349880455485	16.52196377193338	4.019937593710743
9	58.81995380313653	6.49618065405033	16.57287149977712	18.110994043036026
10	39.313682781537466	23.558120922316327	24.137101349434698	12.991094946711513
11	32.29525469060259	20.871408599100377	30.674058840215583	16.15927787008145
12	23.184797584795557	18.494701543947805	33.70775418405803	24.612746687198605
13	26.444918345017626	21.34046885764072	37.422498682984155	14.7921141143575
14	17.13969485755967	32.51661466142562	31.864945090570167	18.478745390444544
15	15.197603031162622	22.368247761073064	40.45670057138226	21.977448636382054
16	17.181991327957206	23.127558050006076	33.5215990598533	26.16885156218341
17	16.90541800056733	28.353578230741178	34.91003768691494	19.830966081776552
18	17.79921586902784	23.15668436195648	33.75992827329092	25.284171495724767
19	18.656542529480895	25.21224216882117	30.974693033999273	25.156522267698666
20	25.04178992584188	24.182690359444017	35.4631843416947	15.312335373019412
21	18.52737366778782	32.487994894030884	32.697451067795924	16.28718037038538
22	17.671060096446084	21.03502249057827	39.16678486039632	22.127132552579326
23	22.57719739028245	26.373495562669692	33.515773797463225	17.533533249584636
24	18.99643392632816	27.964552011994975	31.90901446691251	21.129999594764357
25	15.949315151760748	34.92346111763991	30.6844430036066	18.442780726992748
26	16.483719657981116	23.629037160108606	38.68632734935365	21.200915832556632
27	24.527647201847874	24.81840377679621	31.497447015439477	19.15650200591644
28	15.521285002228796	29.14657373262552	35.79851683754103	19.53362442760465
29	18.356414880252867	21.118855614539854	38.277799165214574	22.24693033999271
30	17.053582283097622	30.12724399238157	35.89374721400495	16.925426510515866
31	21.693023868379463	21.85511812619038	29.429225594683306	27.02263241074685
32	25.356100822628356	25.15120354986425	29.542691575150947	19.950004052356444
33	23.020423876484173	23.101977549945293	31.351562183409655	22.52603639016088
34	18.798375005065445	23.187077035296024	29.158224257405678	28.85632370223285
35	21.685932244600234	25.0043056287231	31.76490254082749	21.54485958584917
36	19.920371195850386	28.239605705717874	30.701665518499006	21.13835757993273
37	17.20427928840621	24.313885399359727	33.962292823276734	24.51954248895733
38	19.919358106739068	23.782773432751146	30.47118774567411	25.826680714835675
39	19.624042630789802	24.446346800664585	29.419347975847955	26.510262592697654
40	17.794150423471248	25.147657737974633	32.690612716294524	24.367579122259595
41	16.465990598533047	25.49210803582283	30.628469830206267	27.413431535437855
42	18.642106009644607	25.342424119625562	32.758236414475014	23.25723345625481
43	21.165964258216153	24.492948899785226	30.159662843943753	24.181423998054868
44	20.083478542772625	23.598897759046885	32.45405640880172	23.86356728937877
45	19.633413705069497	24.080621631478706	29.38794221339709	26.89802245005471
46	24.173066012886494	27.306044089638128	27.584137050694977	20.9367528467804
47	17.50769947724602	26.38084045872675	36.300502492199215	19.810957571828016
48	20.48643473679945	25.228704866880094	30.242736151071846	24.042124245248612
49	18.82446204968189	22.902398995015602	35.74710256514163	22.52603639016088
50	19.62682862584593	23.254194188920856	32.95680188029339	24.162175304939822
51	18.690734286987883	22.95431981197066	30.35063014142724	28.00431575961422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	223.0
1	552.0
2	881.0
3	6215.0
4	11549.0
5	7608.5
6	3668.0
7	3460.0
8	3252.0
9	3213.0
10	3174.0
11	3159.5
12	3145.0
13	3086.0
14	3027.0
15	2854.0
16	2681.0
17	2609.5
18	2538.0
19	2467.0
20	2396.0
21	2254.0
22	2112.0
23	2226.0
24	2340.0
25	2743.5
26	3587.0
27	4027.0
28	4661.5
29	5296.0
30	6228.0
31	7160.0
32	7894.5
33	8629.0
34	10248.0
35	11867.0
36	11624.0
37	11381.0
38	12561.0
39	13741.0
40	14948.0
41	16155.0
42	17779.5
43	19404.0
44	22480.0
45	25556.0
46	40927.5
47	56299.0
48	44176.0
49	32053.0
50	31362.0
51	30671.0
52	28017.5
53	25364.0
54	23439.5
55	21515.0
56	19561.0
57	17607.0
58	15877.0
59	14147.0
60	12310.0
61	10473.0
62	8973.5
63	7474.0
64	6044.5
65	4615.0
66	3690.0
67	2765.0
68	2332.5
69	1900.0
70	1463.0
71	1026.0
72	840.5
73	655.0
74	561.5
75	339.5
76	211.0
77	163.5
78	116.0
79	83.5
80	51.0
81	52.5
82	54.0
83	34.5
84	15.0
85	8.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.35716627012887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.69621192872481	27.884569293816885
2	8.429713432748784	6.1296098576797275
3	3.372662924996167	3.6786140021155447
4	1.89186249605317	2.7513103731690456
5	1.303585309367969	2.369733391999432
6	1.0179174975534735	2.220515742469508
7	0.7930404641467196	2.018289280974572
8	0.6722123843679809	1.9551789941845168
9	0.6059164750809019	1.9826465427294069
>10	5.095849754930862	34.21766098532782
>50	0.07177202248241904	1.6787315983426336
>100	0.03870060035816713	2.5997059589085403
>500	0.0035182363961970115	0.8122482207768763
>1k	0.0042218836754364135	2.2957844199217914
>5k	0.002814589116957609	7.4054013375836965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	8018	2.0307371236373952	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	7663	1.9408254650079022	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6838	1.731875835798517	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	6428	1.628034201888398	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	1788	0.45285083275924953	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	1671	0.42321797625319124	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1576	0.3991571098593832	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC	1378	0.3490091988491308	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTA	1320	0.33431940673501637	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATT	1241	0.31431089678648133	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.2026178222636463	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	718	0.18184949548162255	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	574	0.14537828747416623	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	548	0.1387932082505977	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	535	0.13550066863881347	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCCATCGAATGC	462	0.11701179235725574	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	454	0.11498561413461929	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	438	0.11093325768934635	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.598168334886737E-4	0.0	0.0	0.06686388134700329	0.0
2	7.598168334886737E-4	0.0	0.0	0.6704117194148397	0.0
3	7.598168334886737E-4	0.0	0.0	0.8786015317907363	0.0
4	7.598168334886737E-4	0.0	0.0	1.5388823600923938	0.0
5	7.598168334886737E-4	0.0	0.0	4.120739960286907	0.0
6	7.598168334886737E-4	0.0	0.0	4.7432832191919605	0.0
7	7.598168334886737E-4	0.0	0.0	5.6578494144344935	0.0
8	7.598168334886737E-4	0.0	0.0	6.544302386837947	0.0
9	7.598168334886737E-4	0.0	0.0	6.792509219110913	0.0
10	7.598168334886737E-4	0.0	0.0	9.526076913725332	0.0
11	7.598168334886737E-4	0.0	0.0	10.372259593953885	0.0
12	7.598168334886737E-4	0.0	0.0	12.824695060177493	0.0
13	7.598168334886737E-4	0.0	0.0	13.20460347692183	0.0
14	7.598168334886737E-4	0.0	0.0	13.391265145682214	0.0
15	7.598168334886737E-4	0.0	0.0	13.952009968796855	0.0
16	7.598168334886737E-4	0.0	0.0	14.394983182720752	0.0
17	7.598168334886737E-4	0.0	0.0	14.865056530372412	0.0
18	7.598168334886737E-4	0.0	0.0	15.347033675082061	0.0
19	0.0010130891113182315	0.0	0.0	16.09773270656887	0.0
20	0.0010130891113182315	0.0	0.0	16.477894395591036	0.0
21	0.0010130891113182315	0.0	0.0	16.84843173805568	0.0
22	0.0010130891113182315	0.0	0.0	17.40461766016939	0.0
23	0.0010130891113182315	0.0	0.0	17.798202779916522	0.0
24	0.0010130891113182315	0.0	0.0	18.123657656927502	0.0
25	0.0010130891113182315	0.0	0.0	18.40656279126312	0.0
26	0.0010130891113182315	0.0	0.0	18.684909024597804	0.0
27	0.0010130891113182315	0.0	0.0	19.044555659115776	0.0
28	0.0010130891113182315	0.0	0.0	19.316570085504722	0.0
29	0.0010130891113182315	0.0	0.0	19.621509908011507	0.0
30	0.0010130891113182315	0.0	0.0	19.959628398913967	0.0
31	0.0010130891113182315	0.0	0.0	20.26228877092029	0.0
32	0.0010130891113182315	0.0	0.0	20.569508043927545	0.0
33	0.0010130891113182315	0.0	0.0	20.873688049600844	0.0
34	0.0010130891113182315	0.0	0.0	21.174828787940186	0.0
35	0.0010130891113182315	0.0	0.0	21.474703164890382	0.0
36	0.0010130891113182315	0.0	0.0	21.773311180451433	0.0
37	0.0010130891113182315	0.0	0.0	22.088128621793572	0.0
38	0.0010130891113182315	0.0	0.0	22.372300117518336	0.0
39	0.0010130891113182315	0.0	0.0	22.70180735097459	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	25	3.8861384E-5	45.0	8
AGGTACG	20	7.027654E-4	45.0	1
CCGATCG	20	7.027654E-4	45.0	40
GCGAAAG	35	1.2092278E-7	45.0	1
TAAACGG	35	1.2092278E-7	45.0	2
CGACAGG	25	3.8861384E-5	45.0	2
CTGACGG	35	1.2092278E-7	45.0	2
TTGAGCG	20	7.027654E-4	45.0	1
TCGGATG	25	3.8861384E-5	45.0	21
CTAACGG	45	3.8380676E-10	45.0	2
TCGGAAC	20	7.027654E-4	45.0	35
CTCGGAT	25	3.8861384E-5	45.0	20
CTTGCGG	45	3.8380676E-10	45.0	2
TACGAAT	20	7.027654E-4	45.0	12
CCATACT	20	7.027654E-4	45.0	14
CGAATAC	45	3.8380676E-10	45.0	45
GACGGGT	40	6.7957444E-9	45.0	4
GCGATCG	20	7.027654E-4	45.0	33
ACTCGCG	20	7.027654E-4	45.0	1
CGTAAGC	45	3.8380676E-10	45.0	43
>>END_MODULE