##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799467
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14782473823185	33.0	31.0	34.0	30.0	34.0
2	32.276694347609094	34.0	31.0	34.0	30.0	34.0
3	32.16217805112656	33.0	31.0	34.0	30.0	34.0
4	35.82591901854611	37.0	35.0	37.0	35.0	37.0
5	32.16026677774067	37.0	35.0	37.0	0.0	37.0
6	33.96063001975066	37.0	35.0	37.0	17.0	37.0
7	35.607240824199124	37.0	35.0	37.0	32.0	37.0
8	35.85959145280543	37.0	35.0	37.0	35.0	37.0
9	37.85294202262257	39.0	38.0	39.0	35.0	39.0
10	37.50338287884303	39.0	37.0	39.0	35.0	39.0
11	37.417494405647766	39.0	37.0	39.0	35.0	39.0
12	37.436944864515986	39.0	37.0	39.0	35.0	39.0
13	37.44939315819165	39.0	37.0	39.0	35.0	39.0
14	38.76456814352562	40.0	38.0	41.0	35.0	41.0
15	38.85539615769006	40.0	38.0	41.0	35.0	41.0
16	38.802136923725435	40.0	38.0	41.0	35.0	41.0
17	38.76028654090788	40.0	38.0	41.0	35.0	41.0
18	38.60626642500566	40.0	38.0	41.0	35.0	41.0
19	38.40908380208314	40.0	37.0	41.0	34.0	41.0
20	38.38280754552721	40.0	37.0	41.0	34.0	41.0
21	38.340319237692114	40.0	37.0	41.0	34.0	41.0
22	38.389296869039	40.0	37.0	41.0	34.0	41.0
23	38.376455813685865	40.0	37.0	41.0	35.0	41.0
24	38.34580664367635	40.0	37.0	41.0	34.0	41.0
25	38.13331131866606	40.0	36.0	41.0	34.0	41.0
26	38.17617237484474	40.0	37.0	41.0	34.0	41.0
27	38.13639837541762	40.0	37.0	41.0	34.0	41.0
28	38.08462763316059	40.0	36.0	41.0	34.0	41.0
29	38.07239948615765	40.0	36.0	41.0	34.0	41.0
30	37.883089608451634	40.0	36.0	41.0	34.0	41.0
31	37.81672789496002	40.0	36.0	41.0	33.0	41.0
32	37.603053034083956	40.0	36.0	41.0	33.0	41.0
33	37.384589983076225	40.0	36.0	41.0	33.0	41.0
34	37.12079798165528	40.0	36.0	41.0	32.0	41.0
35	37.02648889822844	40.0	36.0	41.0	31.0	41.0
36	36.89776188385512	40.0	36.0	41.0	31.0	41.0
37	36.79671956440979	40.0	36.0	41.0	31.0	41.0
38	36.6752048552348	40.0	35.0	41.0	30.0	41.0
39	36.564758770530865	40.0	35.0	41.0	30.0	41.0
40	36.50548803140092	40.0	35.0	41.0	30.0	41.0
41	36.40027543350757	40.0	35.0	41.0	30.0	41.0
42	36.36872941597339	40.0	35.0	41.0	30.0	41.0
43	36.284494544490265	40.0	35.0	41.0	29.0	41.0
44	36.23144670136478	39.0	35.0	41.0	29.0	41.0
45	36.23956961325483	39.0	35.0	41.0	29.0	41.0
46	36.13369032117648	39.0	35.0	41.0	29.0	41.0
47	36.06021636915595	39.0	35.0	41.0	29.0	41.0
48	36.02672280406821	39.0	35.0	41.0	28.0	41.0
49	35.99991119083089	39.0	35.0	41.0	28.0	41.0
50	35.88436545848672	39.0	35.0	41.0	28.0	41.0
51	34.93995499501543	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	19.0
10	27.0
11	22.0
12	13.0
13	15.0
14	12.0
15	25.0
16	41.0
17	96.0
18	186.0
19	415.0
20	799.0
21	1368.0
22	2183.0
23	3161.0
24	4785.0
25	7512.0
26	10291.0
27	11072.0
28	10671.0
29	10305.0
30	11072.0
31	13034.0
32	16396.0
33	23259.0
34	37816.0
35	52443.0
36	54985.0
37	86075.0
38	153291.0
39	287830.0
40	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90095901394304	20.30315197500335	24.446412422276342	15.349476588777272
2	35.187693800994914	24.739983013682867	23.905802240742897	16.166520944579325
3	29.085378133181234	25.0330532717423	30.660427509828423	15.221141085248046
4	26.310779556879773	24.54035000819296	32.020458630562615	17.128411804364656
5	23.10114113528138	35.13340763283538	25.42806644927183	16.337384782611412
6	23.7754654038253	33.775628012163104	27.81528193158692	14.633624652424679
7	77.0283201182788	4.847354549968917	14.393714812493824	3.7306105192584558
8	78.63914332924311	5.857777744422221	11.959718162225583	3.5433607641090874
9	72.55959282872215	7.365282119211924	13.461969036870816	6.613156015195123
10	34.12561118845431	34.65183678625884	19.765293626878908	11.457258398407939
11	24.68244467876723	24.656552428055193	33.09167232668766	17.569330566489924
12	22.937532130782134	23.222972305298406	34.558024283679	19.28147128024046
13	22.254952362011192	24.10531016289603	36.04776682464692	17.59197065044586
14	18.706213014420857	28.3287490290406	33.02913065830109	19.935907298237453
15	17.738067987796867	26.32679022398673	37.47121519712508	18.463926591091315
16	20.922564658703863	26.64087448262405	33.44678391978656	18.989776938885534
17	20.536807648095543	26.1021405511422	32.967089323261625	20.393962477500637
18	21.658304845603382	24.834295849609802	34.03667693600861	19.470722368778198
19	20.504411063871306	26.903549489847613	32.28976305463515	20.302276391645936
20	23.040850966956736	26.809236653920678	33.18723599598232	16.96267638314027
21	22.080836357223	27.386871503138966	33.11068499387717	17.421607145760866
22	21.48168717407973	23.799606487822512	34.05931701996455	20.659389318133208
23	19.567912121450917	26.93507049071444	33.68531784301291	19.811699544821735
24	19.857605129417475	25.941908796735824	34.097092187670036	20.103393886176665
25	19.053944690650145	28.74264979042287	32.05410604815459	20.1492994707724
26	19.501117619614067	27.837046432185442	33.14470766147946	19.51712828672103
27	20.24836547349672	26.82499715435409	33.14595849484719	19.780678877302
28	17.931947159795214	27.071286244460374	34.863978125426065	20.13278847031835
29	19.24982519603686	25.08321168978832	35.61910622952542	20.0478568846494
30	19.743529126280386	25.882744378442133	34.785550873269315	19.588175622008166
31	21.54935725927399	26.14454380230829	31.803313957924466	20.502784980493256
32	21.37836833790513	26.93907315749118	32.78121548481676	18.901343019786935
33	21.345721587007343	26.12515588510845	31.063195854237886	21.465926673646315
34	19.78618254412002	26.89829598970314	32.65287998128753	20.662641484889306
35	20.64913248451781	25.992317381455393	32.81248631901004	20.546063815016755
36	21.761373515104438	27.81740834831206	30.9188496836017	19.5023684529818
37	21.184364082570013	27.037888993541948	32.31390413863237	19.46384278525568
38	21.25466091783651	27.26766708319418	29.995234324868942	21.482437674100368
39	21.80302626624989	26.44274247717542	30.727722345012364	21.026508911562328
40	22.00365993843398	26.011580215318457	31.283967943642455	20.70079190260511
41	21.245654917588844	26.908802989992083	30.369483668494134	21.47605842392494
42	22.72839279169747	25.718009623911932	31.181774857498805	20.371822726891793
43	22.481103034897	25.414557448900332	30.893082516226432	21.211256999976232
44	21.095179663450775	26.051857049759402	31.01341268620218	21.839550600587643
45	21.07491616289353	25.5431431191031	30.31457208365073	23.067368634352636
46	22.18002744328409	26.28538763951483	30.21613149761028	21.318453419590803
47	20.151300804160773	26.76433173601912	32.299019221556364	20.785348238263744
48	21.495321257788003	25.569535703162234	31.511744699906313	21.423398339143453
49	21.623906927990774	25.0944691900979	32.20720805236489	21.074415829546435
50	20.29977472491047	25.03067668834361	32.053855881481034	22.615692705264884
51	20.136791137095088	24.62565684387223	30.43277583690134	24.804776182131345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	329.0
1	814.0
2	1299.0
3	8512.5
4	15726.0
5	10485.0
6	5244.0
7	5016.5
8	4789.0
9	4782.5
10	4776.0
11	4728.5
12	4681.0
13	4503.5
14	4326.0
15	4165.5
16	4005.0
17	4155.0
18	4305.0
19	4240.0
20	4175.0
21	4344.0
22	4513.0
23	5039.5
24	5566.0
25	6510.0
26	8722.5
27	9991.0
28	11647.5
29	13304.0
30	15841.5
31	18379.0
32	20495.5
33	22612.0
34	24712.0
35	26812.0
36	29624.0
37	32436.0
38	34644.0
39	36852.0
40	39978.5
41	43105.0
42	47194.0
43	51283.0
44	54037.5
45	56792.0
46	66913.0
47	77034.0
48	74532.5
49	72031.0
50	69445.0
51	66859.0
52	58650.0
53	50441.0
54	44625.0
55	38809.0
56	34465.5
57	30122.0
58	26460.0
59	22798.0
60	20976.0
61	19154.0
62	16851.0
63	14548.0
64	12347.0
65	10146.0
66	7916.0
67	5686.0
68	4499.5
69	3313.0
70	2908.5
71	2504.0
72	1850.5
73	1197.0
74	1096.5
75	733.5
76	471.0
77	410.5
78	350.0
79	224.0
80	98.0
81	97.0
82	96.0
83	70.0
84	44.0
85	26.5
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	799467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.28592370800146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16512355453956	25.428197679854453
2	9.502757188981862	6.516216159941892
3	3.686435060471951	3.791784937135292
4	1.9996673931848965	2.7424177473646205
5	1.311115531898693	2.247640354952717
6	0.9851565170197844	2.026620070978846
7	0.7649258159562936	1.8358331717710772
8	0.6335861932336542	1.737847030692171
9	0.5453848906299331	1.6829122276471147
>10	6.250128838145565	43.97167647066704
>50	0.1183612490398654	2.5591548978797642
>100	0.031809583695291035	1.931033180728609
>500	0.0025891521612446083	0.5715614727486581
>1k	0.0025891521612446083	1.875487601615244
>5k	3.6987888017780117E-4	1.081616996022477
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8529	1.066835779337984	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	3499	0.43766659536916475	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	3203	0.40064192768431967	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	2907	0.36361725999947464	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1656	0.20713800569629515	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1298	0.16235817113151638	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC	1225	0.15322708754707823	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1001	0.12520842010989822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.254166838656254E-4	0.0	0.0	0.10356900284814757	0.0
2	6.254166838656254E-4	0.0	0.0	0.593020099641386	0.0
3	6.254166838656254E-4	0.0	0.0	0.8358068563180219	0.0
4	6.254166838656254E-4	0.0	0.0	1.1704047821861314	0.0
5	6.254166838656254E-4	0.0	0.0	2.2546271453355797	0.0
6	6.254166838656254E-4	0.0	0.0	2.910814330047394	0.0
7	6.254166838656254E-4	0.0	0.0	3.5828870985293952	0.0
8	6.254166838656254E-4	0.0	0.0	4.473355373017273	0.0
9	6.254166838656254E-4	0.0	0.0	4.841725799814126	0.0
10	6.254166838656254E-4	0.0	0.0	6.076548500438417	0.0
11	6.254166838656254E-4	0.0	0.0	7.257460282913491	0.0
12	6.254166838656254E-4	0.0	0.0	8.513547150789213	0.0
13	6.254166838656254E-4	0.0	0.0	8.920318161975416	0.0
14	6.254166838656254E-4	0.0	0.0	9.10218933364354	0.0
15	6.254166838656254E-4	0.0	0.0	9.435911676154237	0.0
16	7.505000206387506E-4	0.0	0.0	9.951755357006606	0.0
17	7.505000206387506E-4	0.0	0.0	10.590806124580501	0.0
18	7.505000206387506E-4	0.0	0.0	11.226729808735069	0.0
19	8.755833574118757E-4	0.0	0.0	11.780098490619375	0.0
20	8.755833574118757E-4	0.0	0.0	12.214137669222119	0.0
21	8.755833574118757E-4	0.0	0.0	12.727229516665478	0.0
22	8.755833574118757E-4	0.0	0.0	13.318373366255267	0.0
23	8.755833574118757E-4	0.0	0.0	13.868740048057019	0.0
24	8.755833574118757E-4	0.0	0.0	14.305280893395224	0.0
25	8.755833574118757E-4	0.0	0.0	14.697792404189292	0.0
26	8.755833574118757E-4	0.0	0.0	15.076919997948634	0.0
27	8.755833574118757E-4	0.0	0.0	15.465679008639507	0.0
28	0.0010006666941850008	0.0	0.0	15.878704186664365	0.0
29	0.0010006666941850008	0.0	0.0	16.287726697912483	0.0
30	0.0010006666941850008	0.0	0.0	16.76817179445806	0.0
31	0.0010006666941850008	0.0	0.0	17.212467806676198	0.0
32	0.0010006666941850008	0.0	0.0	17.628244818110065	0.0
33	0.0010006666941850008	0.0	0.0	18.0246339123441	0.0
34	0.0012508333677312509	0.0	0.0	18.41614475644398	0.0
35	0.001375916704504376	0.0	0.0	18.835424101307495	0.0
36	0.001375916704504376	0.0	0.0	19.2705890299412	0.0
37	0.001375916704504376	0.0	0.0	19.686616208048612	0.0
38	0.001375916704504376	0.0	0.0	20.111399219730146	0.0
39	0.001375916704504376	0.0	0.0	20.543687231618065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.0319977E-4	45.0	1
ACCGGTC	20	7.0319977E-4	45.0	39
TCCGCGG	20	7.0319977E-4	45.0	2
CGTGATA	25	3.8897364E-5	45.0	22
CGCATCG	20	7.0319977E-4	45.0	21
CGCATAT	20	7.0319977E-4	45.0	25
CACGAAC	20	7.0319977E-4	45.0	42
ATCGTTC	20	7.0319977E-4	45.0	22
TACGAAG	40	6.8102963E-9	45.0	1
TTACCGG	20	7.0319977E-4	45.0	2
TCGACGT	20	7.0319977E-4	45.0	26
TCGACGG	25	3.8897364E-5	45.0	2
CGTCATA	20	7.0319977E-4	45.0	38
TACGTAG	25	3.8897364E-5	45.0	1
GTACGCG	20	7.0319977E-4	45.0	1
CGGACTC	40	6.8102963E-9	45.0	6
CGTTTTT	4575	0.0	43.327873	1
CGTTATT	625	0.0	41.76	1
CGGTCGA	60	3.6379788E-12	41.249996	41
GCGATAC	55	6.002665E-11	40.909092	9
>>END_MODULE