##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	828266
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14304704044353	33.0	31.0	34.0	30.0	34.0
2	32.27503000243883	34.0	31.0	34.0	30.0	34.0
3	32.167923106828	33.0	31.0	34.0	30.0	34.0
4	35.808667746834956	37.0	35.0	37.0	35.0	37.0
5	32.071969632944004	37.0	35.0	37.0	0.0	37.0
6	33.89262628189495	37.0	35.0	37.0	17.0	37.0
7	35.56774393733414	37.0	35.0	37.0	32.0	37.0
8	35.828598542014284	37.0	35.0	37.0	35.0	37.0
9	37.804072604694625	39.0	38.0	39.0	35.0	39.0
10	37.413586939461474	39.0	37.0	39.0	34.0	39.0
11	37.3901222554107	39.0	37.0	39.0	35.0	39.0
12	37.385729946659644	39.0	37.0	39.0	35.0	39.0
13	37.38404329044051	39.0	37.0	39.0	35.0	39.0
14	38.676064211255806	40.0	38.0	41.0	35.0	41.0
15	38.78939374548756	40.0	38.0	41.0	35.0	41.0
16	38.71322739313216	40.0	38.0	41.0	35.0	41.0
17	38.69775410315044	40.0	38.0	41.0	35.0	41.0
18	38.55207022864635	40.0	38.0	41.0	35.0	41.0
19	38.36788543776999	40.0	37.0	41.0	34.0	41.0
20	38.391510698253946	40.0	37.0	41.0	34.0	41.0
21	38.29753122789056	40.0	37.0	41.0	34.0	41.0
22	38.356783931732075	40.0	37.0	41.0	34.0	41.0
23	38.35745883568805	40.0	37.0	41.0	34.0	41.0
24	38.32934588646643	40.0	37.0	41.0	34.0	41.0
25	38.07088423284307	40.0	36.0	41.0	34.0	41.0
26	38.129062402658086	40.0	36.0	41.0	34.0	41.0
27	38.12882093433752	40.0	36.0	41.0	34.0	41.0
28	38.068486452419876	40.0	36.0	41.0	34.0	41.0
29	38.04096389324203	40.0	36.0	41.0	34.0	41.0
30	37.84819248888642	40.0	36.0	41.0	34.0	41.0
31	37.79321015229407	40.0	36.0	41.0	33.0	41.0
32	37.643263154590436	40.0	36.0	41.0	33.0	41.0
33	37.451487807057156	40.0	36.0	41.0	33.0	41.0
34	37.26133271195486	40.0	36.0	41.0	33.0	41.0
35	37.160827560228235	40.0	36.0	41.0	32.0	41.0
36	37.034917526495114	40.0	36.0	41.0	32.0	41.0
37	36.9865055429053	40.0	36.0	41.0	32.0	41.0
38	36.871672868378035	40.0	35.0	41.0	31.0	41.0
39	36.79968633265159	40.0	35.0	41.0	31.0	41.0
40	36.74424037688375	40.0	35.0	41.0	31.0	41.0
41	36.64122153994007	40.0	35.0	41.0	30.0	41.0
42	36.60872594070021	40.0	35.0	41.0	30.0	41.0
43	36.549566202162104	40.0	35.0	41.0	30.0	41.0
44	36.49311694552233	40.0	35.0	41.0	30.0	41.0
45	36.46783279767611	40.0	35.0	41.0	30.0	41.0
46	36.350753260425996	39.0	35.0	41.0	30.0	41.0
47	36.299185285886416	39.0	35.0	41.0	30.0	41.0
48	36.255852588419664	39.0	35.0	41.0	30.0	41.0
49	36.21237863198537	39.0	35.0	41.0	30.0	41.0
50	36.11907648026117	39.0	35.0	41.0	29.0	41.0
51	35.17572494826541	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	11.0
9	23.0
10	30.0
11	19.0
12	22.0
13	17.0
14	29.0
15	28.0
16	48.0
17	106.0
18	191.0
19	359.0
20	684.0
21	1101.0
22	1718.0
23	2655.0
24	4478.0
25	7014.0
26	9576.0
27	10560.0
28	10465.0
29	10345.0
30	11252.0
31	13441.0
32	17024.0
33	24898.0
34	41901.0
35	58765.0
36	57483.0
37	90144.0
38	156852.0
39	296778.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.60164971156609	21.272996839179683	24.532819166789412	14.592534282464811
2	35.953304856169396	23.413251298495894	24.401943337043896	16.231500508290814
3	31.219439165678658	22.94299174419812	29.963441696266656	15.874127393856563
4	27.410759345427678	24.628078419251786	29.78451367072897	18.17664856459157
5	21.984241777400012	36.47765331427343	25.122001868964798	16.41610303936175
6	23.9145395319861	33.93692364530235	28.354779744671397	13.793757078040148
7	76.83932456481372	6.259221071491526	12.600420637814421	4.301033725880333
8	78.74137052589386	5.644080524855542	11.72063081184064	3.8939181374099623
9	72.39280617579377	7.8990324364395015	13.800759659336492	5.907401728430239
10	36.762585932538585	27.966498685204993	21.620107549989978	13.650807832266446
11	27.025858842449164	25.330147561290694	29.05829769663369	18.585695899626447
12	25.63439764520094	22.44423892807383	32.442596943494	19.478766483231233
13	23.310868730576892	23.57696681983807	33.55371342056779	19.55845102901725
14	19.469107750408686	26.778957484672798	32.75747163350904	20.994463131409475
15	18.964438960430584	26.502476257627382	35.5284413461376	19.00464343580444
16	21.89465703047089	25.805840152801153	33.14019892160248	19.15930389512548
17	21.481383999826143	26.100914440529976	31.044857569911116	21.372843989732768
18	21.82125066101953	25.674843588895357	32.74902024228931	19.754885507795805
19	22.26398282677304	27.934262664409744	30.497931823834374	19.303822684982844
20	23.937237554119086	26.09717168156124	31.23984323876629	18.725747525553384
21	22.9770387773976	27.236177749660133	31.31578502558357	18.4709984473587
22	21.839602253382367	25.223418563601545	31.463805106089104	21.473174076926977
23	21.105055622227642	26.51141058548824	31.902070107912195	20.481463684371928
24	21.351836245843728	25.586224715248484	32.702054653939676	20.35988438496811
25	20.519857147341554	27.65741923488348	30.44939669140107	21.373326926373895
26	20.014946889042893	28.009842248746175	31.130216621230378	20.844994240980554
27	21.061470590365897	27.365121832841137	31.605426276099706	19.967981300693253
28	19.38435236989083	27.195852540125998	32.56405550873753	20.85573958124564
29	20.407574378279442	25.937561121668644	32.32765802290569	21.32720647714623
30	21.549478066225102	26.680800612363658	31.505216923065777	20.26450439834546
31	21.574349303243164	26.572502070590847	29.97020280924244	21.88294581692355
32	21.824148280866293	27.532097176510927	29.951971951039884	20.691782591582896
33	22.239594526396107	26.809503227224102	29.852124800486802	21.098777445892985
34	20.477962393723757	26.538937974032496	30.39216869942748	22.59093093281627
35	21.424518210333396	26.034993589016086	30.516162682036928	22.024325518613587
36	21.535231435311843	27.20478686798685	30.33180161928656	20.928180077414744
37	20.82628044613687	28.112587019146023	30.241975404036868	20.819157130680242
38	19.99526722091695	28.056928571256094	29.836912296291292	22.110891911535667
39	21.716091207414042	26.916352959073535	29.406495014886524	21.9610608186259
40	21.934016366722766	25.80970364593017	31.061398149869728	21.194881837477332
41	19.536718880166518	25.928023123006376	31.701772136004617	22.83348586082249
42	20.092337485783553	26.458166820803946	31.733283751838176	21.716211941574326
43	21.178582725839284	26.275858238778365	30.29847899104877	22.247080044333583
44	21.001707181026386	26.70905240586961	29.69287644307505	22.59636397002895
45	21.16337022164377	26.27935952942654	28.859207066328935	23.69806318260076
46	21.481504733986426	27.20659788039108	29.24096848114012	22.070928904482376
47	20.67427613834203	26.47289638835833	30.849026761933967	22.003800711365674
48	20.017844508889656	25.863188878935027	31.53105403336609	22.587912578809224
49	20.64288525666875	25.4012599816967	31.45800986639558	22.49784489523897
50	19.768166265426807	25.520062395414033	31.058862732503812	23.65290860665535
51	19.403911303856493	25.32121323342984	30.254773225026742	25.020102237686924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	464.0
1	953.0
2	1442.0
3	8525.0
4	15608.0
5	10330.0
6	5052.0
7	4748.5
8	4445.0
9	4438.5
10	4432.0
11	4408.0
12	4384.0
13	4256.0
14	4128.0
15	3843.5
16	3559.0
17	3568.5
18	3578.0
19	3556.5
20	3535.0
21	4043.5
22	4552.0
23	4794.5
24	5037.0
25	5744.0
26	7800.5
27	9150.0
28	11487.0
29	13824.0
30	15424.5
31	17025.0
32	19614.0
33	22203.0
34	24846.5
35	27490.0
36	28950.0
37	30410.0
38	32232.5
39	34055.0
40	37793.5
41	41532.0
42	46193.0
43	50854.0
44	53476.5
45	56099.0
46	62201.5
47	68304.0
48	71351.0
49	74398.0
50	71170.0
51	67942.0
52	60564.5
53	53187.0
54	47589.0
55	41991.0
56	38450.5
57	34910.0
58	32699.5
59	30489.0
60	29384.5
61	28280.0
62	24745.0
63	21210.0
64	18270.5
65	15331.0
66	12193.5
67	9056.0
68	7325.5
69	5595.0
70	4589.5
71	3584.0
72	2722.0
73	1860.0
74	1620.5
75	1019.5
76	658.0
77	570.0
78	482.0
79	340.5
80	199.0
81	122.0
82	45.0
83	30.0
84	15.0
85	17.0
86	19.0
87	17.5
88	16.0
89	9.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	828266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.266935606139306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2481202573421	24.700074354778863
2	9.339847494662422	6.214162103521925
3	3.66687857813552	3.659574406030979
4	2.050916432209037	2.7291081953548395
5	1.3415252741121055	2.2314217453947895
6	0.9935545481443495	1.983150910458294
7	0.7732095912113099	1.8005619576613157
8	0.616383411734103	1.6404149813480686
9	0.5313844087174819	1.590977781621979
>10	6.199932447414485	45.285913947839305
>50	0.19890729904179358	4.003612701656987
>100	0.035295931761043176	2.0044358927714168
>500	0.002573661690909381	0.6795610092895988
>1k	0.0011029978675325919	0.42038358332043757
>5k	3.6766595584419734E-4	1.0566464289511959
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8639	1.0430224106748316	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	1244	0.15019329539061121	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC	1134	0.13691253775960863	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1059	0.12785747573847048	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG	994	0.12000975532015079	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	977	0.11795727459535947	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC	858	0.10358990952182028	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4146832056368364E-4	0.0	0.0	0.10165816295731081	0.0
2	3.6220248084552545E-4	0.0	0.0	0.4523908985760613	0.0
3	3.6220248084552545E-4	0.0	0.0	0.6797333223867694	0.0
4	3.6220248084552545E-4	0.0	0.0	0.9435374626025939	0.0
5	4.829366411273673E-4	0.0	0.0	1.7222727964204736	0.0
6	4.829366411273673E-4	0.0	0.0	2.378583691712566	0.0
7	4.829366411273673E-4	0.0	0.0	2.944706169274122	0.0
8	4.829366411273673E-4	0.0	0.0	3.7764437994557305	0.0
9	4.829366411273673E-4	0.0	0.0	4.122950839464616	0.0
10	4.829366411273673E-4	0.0	0.0	4.936578345603949	0.0
11	4.829366411273673E-4	0.0	0.0	6.02282358565968	0.0
12	4.829366411273673E-4	0.0	0.0	6.955132771356062	0.0
13	4.829366411273673E-4	0.0	0.0	7.32735618750498	0.0
14	4.829366411273673E-4	0.0	0.0	7.483948393390529	0.0
15	4.829366411273673E-4	0.0	0.0	7.748356204407763	0.0
16	4.829366411273673E-4	0.0	0.0	8.245298008127824	0.0
17	4.829366411273673E-4	0.0	0.0	8.85271156850577	0.0
18	4.829366411273673E-4	0.0	0.0	9.477148645483457	0.0
19	4.829366411273673E-4	0.0	0.0	9.922899165244015	0.0
20	4.829366411273673E-4	0.0	0.0	10.32373657737973	0.0
21	4.829366411273673E-4	0.0	0.0	10.818988102855846	0.0
22	4.829366411273673E-4	0.0	0.0	11.352633091301586	0.0
23	4.829366411273673E-4	0.0	0.0	11.88639881390761	0.0
24	4.829366411273673E-4	0.0	0.0	12.31114158977913	0.0
25	4.829366411273673E-4	0.0	0.0	12.694110346193131	0.0
26	4.829366411273673E-4	0.0	0.0	13.07031798963135	0.0
27	4.829366411273673E-4	0.0	0.0	13.427087433264193	0.0
28	4.829366411273673E-4	0.0	0.0	13.802570671740721	0.0
29	4.829366411273673E-4	0.0	0.0	14.195681097618397	0.0
30	4.829366411273673E-4	0.0	0.0	14.64155235153924	0.0
31	4.829366411273673E-4	0.0	0.0	15.067140266532732	0.0
32	4.829366411273673E-4	0.0	0.0	15.463269046417455	0.0
33	4.829366411273673E-4	0.0	0.0	15.861450307026969	0.0
34	4.829366411273673E-4	0.0	0.0	16.244781265921816	0.0
35	6.036708014092091E-4	0.0	0.0	16.64078931164626	0.0
36	7.244049616910509E-4	0.0	0.0	17.04597315355212	0.0
37	7.244049616910509E-4	0.0	0.0	17.430269985729222	0.0
38	7.244049616910509E-4	0.0	0.0	17.824587753209716	0.0
39	7.244049616910509E-4	0.0	0.0	18.237619315533898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.032145E-4	45.0	4
ATGTCGC	20	7.032145E-4	45.0	27
ACGCTTT	20	7.032145E-4	45.0	5
CCGACCG	25	3.8898586E-5	45.0	15
CGTAAGG	50	2.1827873E-11	45.0	2
ATGCGCG	30	2.1647647E-6	44.999996	1
CGTTTTT	4040	0.0	43.217823	1
CGTTAGG	95	0.0	42.63158	2
CGTTATT	555	0.0	41.756756	1
GCGTTAG	85	0.0	39.705883	1
ATGGGCG	230	0.0	39.130436	5
CGGGTTA	35	6.246177E-6	38.571426	6
CGCTGTT	35	6.246177E-6	38.571426	13
CCGACGG	35	6.246177E-6	38.571426	2
CTTACGG	65	9.094947E-12	38.07692	2
CCGCTCG	30	1.1396469E-4	37.499996	19
ACGGGCG	120	0.0	37.499996	5
TTAACGG	60	1.546141E-10	37.499996	2
TATAGCG	60	1.546141E-10	37.499996	1
GCGTAAG	30	1.1396469E-4	37.499996	1
>>END_MODULE