FastQCFastQC Report
Sat 14 Jan 2017
SRR2936020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095821
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100480.9169380765654245No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC14410.1314995788545757RNA PCR Primer, Index 40 (96% over 27bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12290.11215335351302813No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.11023698213485597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG207.033143E-445.022
GTCTCGA253.8906863E-545.045
GTACGCG207.033143E-445.01
CGTTTTT39200.041.7857131
CGTTATT5900.041.186441
TACGGGT2000.040.54
TCGTAAG850.039.7058831
TCGTGCG403.4580262E-739.3751
CTTGCGG750.039.02
GTTACGG1350.038.3333322
CGGGCAC2300.038.1521726
CGTAAGG2250.038.02
TATACGG1250.037.8000032
TATGGGC3950.037.5949364
ACGGGTC2400.037.5000045
ACTAACG301.1398871E-437.5000041
TGCACGG1150.037.1739122
CGTATGG1100.036.818182
GTATACG552.748493E-936.818181
TACGGGC2450.036.7346954
Produced by FastQC (version 0.11.5)