##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936020.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1095821
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.174234660587814	33.0	31.0	34.0	30.0	34.0
2	32.297356046288584	34.0	31.0	34.0	30.0	34.0
3	32.21913615453619	34.0	31.0	34.0	30.0	34.0
4	35.84044383161118	37.0	35.0	37.0	35.0	37.0
5	32.03858933165179	37.0	35.0	37.0	0.0	37.0
6	33.888297450039744	37.0	35.0	37.0	17.0	37.0
7	35.57063699272052	37.0	35.0	37.0	32.0	37.0
8	35.821330308508415	37.0	35.0	37.0	35.0	37.0
9	37.7992226832667	39.0	38.0	39.0	35.0	39.0
10	37.39863536106718	39.0	37.0	39.0	34.0	39.0
11	37.4094135812327	39.0	37.0	39.0	35.0	39.0
12	37.4014496893197	39.0	37.0	39.0	35.0	39.0
13	37.40316803565546	39.0	37.0	39.0	35.0	39.0
14	38.68170896524158	40.0	38.0	41.0	35.0	41.0
15	38.81739444672077	40.0	38.0	41.0	35.0	41.0
16	38.73001977512751	40.0	38.0	41.0	35.0	41.0
17	38.72561394607331	40.0	38.0	41.0	35.0	41.0
18	38.61298971273593	40.0	38.0	41.0	35.0	41.0
19	38.455959504335105	40.0	37.0	41.0	34.0	41.0
20	38.51462693268335	40.0	38.0	41.0	35.0	41.0
21	38.420945574140305	40.0	37.0	41.0	34.0	41.0
22	38.47806895469242	40.0	37.0	41.0	35.0	41.0
23	38.49205572807968	40.0	37.0	41.0	35.0	41.0
24	38.46274893436063	40.0	37.0	41.0	35.0	41.0
25	38.19358271104496	40.0	37.0	41.0	34.0	41.0
26	38.268547509127856	40.0	37.0	41.0	34.0	41.0
27	38.263834148095356	40.0	37.0	41.0	34.0	41.0
28	38.18280175320604	40.0	37.0	41.0	34.0	41.0
29	38.12449752286185	40.0	37.0	41.0	34.0	41.0
30	37.94825432255816	40.0	36.0	41.0	34.0	41.0
31	37.91818189284564	40.0	37.0	41.0	34.0	41.0
32	37.84160186745828	40.0	37.0	41.0	34.0	41.0
33	37.713396622258564	40.0	36.0	41.0	33.0	41.0
34	37.5935221172071	40.0	36.0	41.0	33.0	41.0
35	37.54666683701079	40.0	36.0	41.0	33.0	41.0
36	37.43650924740446	40.0	36.0	41.0	33.0	41.0
37	37.39949681562956	40.0	36.0	41.0	33.0	41.0
38	37.28290113075037	40.0	36.0	41.0	33.0	41.0
39	37.18952639162783	40.0	36.0	41.0	32.0	41.0
40	37.14183520848752	40.0	35.0	41.0	32.0	41.0
41	37.036469459884415	40.0	35.0	41.0	32.0	41.0
42	37.00840830756118	40.0	35.0	41.0	32.0	41.0
43	36.948301775563706	40.0	35.0	41.0	32.0	41.0
44	36.88145235398847	40.0	35.0	41.0	31.0	41.0
45	36.85991142714002	40.0	35.0	41.0	31.0	41.0
46	36.73037293499577	39.0	35.0	41.0	31.0	41.0
47	36.69702989813117	39.0	35.0	41.0	31.0	41.0
48	36.64064203916516	39.0	35.0	41.0	31.0	41.0
49	36.578573507899556	39.0	35.0	41.0	31.0	41.0
50	36.495830979694674	39.0	35.0	41.0	31.0	41.0
51	35.549880865579325	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	18.0
9	41.0
10	46.0
11	38.0
12	26.0
13	31.0
14	28.0
15	61.0
16	66.0
17	132.0
18	208.0
19	434.0
20	684.0
21	1163.0
22	1865.0
23	2812.0
24	4484.0
25	6888.0
26	9441.0
27	11005.0
28	11585.0
29	12144.0
30	14130.0
31	17121.0
32	22131.0
33	32805.0
34	56663.0
35	76723.0
36	78224.0
37	122849.0
38	210826.0
39	400787.0
40	361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16593859763593	21.11512737938039	24.249763419390575	13.469170603593106
2	34.433999713456856	23.951083251735454	24.65658168624255	16.95833534856514
3	32.841403842415865	23.41751070658438	27.75279904290938	15.98828640809037
4	29.463114870038083	24.652931455046033	27.33950161568358	18.544452059232302
5	22.162743732781177	37.80060794600578	23.03870796416568	16.997940357047366
6	24.721099522641016	35.2511039667975	25.91947042445801	14.108326086103478
7	78.51364410793369	6.800106951774058	10.63138961563978	4.054859324652475
8	80.30755022946265	6.094973540386614	9.759440638571446	3.8380355915792816
9	73.6483422018742	8.64639389097307	12.012180821502783	5.6930830856499375
10	37.62795201041046	26.885869133736257	20.766621555892797	14.719557299960487
11	28.2296104929546	27.25518127504401	26.050422468633105	18.464785763368287
12	26.43679944078458	23.507671417138383	30.23349616406329	19.822032978013745
13	23.79768228570177	24.431636188757107	31.510438292385345	20.26024323315578
14	20.221185759353034	26.903755266599198	31.068486550266876	21.80657242378089
15	20.116150356673216	26.76486396957167	33.09655500305251	20.022430670702608
16	22.522382761418154	25.67125470309476	31.779004052669187	20.027358482817906
17	22.285482756764107	26.183382139966287	29.515678199267946	22.015456904001656
18	22.367521702905858	26.51473187683025	30.78951763107296	20.328228789190934
19	23.086069713940507	27.22999467978803	29.152388939434452	20.531546666837013
20	23.999448815089323	26.655448289456036	29.798936140117778	19.546166755336866
21	23.558774653889643	27.707353664512723	29.728395422245057	19.005476259352577
22	22.54574424107587	25.595512405767	30.018315035028532	21.8404283181286
23	21.75784183730737	26.453042969609086	30.415825212329384	21.373289980754155
24	20.935170981392034	26.15436280195397	31.64850828739365	21.261957929260344
25	20.82566404549648	28.016163223738182	29.436194414963758	21.721978315801575
26	20.409537689093384	27.84131714942495	29.966938030937534	21.782207130544133
27	21.135021139401417	27.921713491528273	30.19398241136098	20.749282957709333
28	20.166706058744996	27.580051851534147	31.1397573143789	21.11348477534196
29	20.571699209998716	26.843070172957077	30.931146601497872	21.654084015546335
30	20.72938919768831	27.390969875554493	30.418015351047295	21.461625575709903
31	21.604714638613423	28.41896623627399	28.739730302668043	21.236588822444542
32	21.860687101269278	27.946717575224422	28.821404225690145	21.37119109781616
33	21.242885471258536	27.99453560389881	28.4626777548523	22.299901169990356
34	20.5645812591655	28.377536112193507	28.940219251136817	22.117663377504172
35	21.43662149201375	26.710658036303375	29.069437435493573	22.783283036189307
36	20.7595948608395	29.432909206886894	27.99243672096081	21.815059211312796
37	20.52223857728589	29.464574962516686	28.71144101089503	21.301745449302395
38	21.01620611395474	28.526465545011458	28.107601515210966	22.34972682582283
39	21.532348805142444	27.839583289606608	28.275420894470905	22.352647010780043
40	22.0037761641728	26.989535699717383	29.491312906031187	21.515375230078636
41	19.614243567151934	27.364596955159648	28.862469326650974	24.15869015103744
42	19.67885265933031	27.67888186117988	28.873785043360183	23.768480436129625
43	20.901497598604152	26.79278823822504	28.955002687482718	23.35071147568809
44	20.7082178567485	27.52383829110776	28.40947563516304	23.358468216980693
45	20.675639543319573	27.73409160802722	27.68134576723753	23.90892308141567
46	20.389552673292442	28.42635795444694	28.703684269602427	22.480405102658192
47	20.60728896416477	26.706642781987206	29.978801282326216	22.707266971521808
48	19.79383494202064	26.823450180275792	30.098802632911763	23.283912244791804
49	20.287893734469407	26.731646865683356	29.847575470811382	23.132883929035856
50	19.726488176444875	26.56711269450029	30.116049975315313	23.590349153739528
51	19.914201315725837	25.695163717431953	29.042790747758986	25.34784421908323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	535.0
1	1295.0
2	2055.0
3	9511.5
4	16968.0
5	10722.5
6	4477.0
7	4325.5
8	4174.0
9	4103.0
10	4032.0
11	4005.5
12	3979.0
13	3835.0
14	3691.0
15	3630.0
16	3569.0
17	3633.0
18	3697.0
19	3854.0
20	4011.0
21	5333.0
22	6655.0
23	6460.0
24	6265.0
25	7531.5
26	10662.5
27	12527.0
28	15425.0
29	18323.0
30	21619.0
31	24915.0
32	27868.5
33	30822.0
34	33647.0
35	36472.0
36	38035.5
37	39599.0
38	42855.5
39	46112.0
40	50696.5
41	55281.0
42	58291.0
43	61301.0
44	67799.0
45	74297.0
46	80351.5
47	86406.0
48	89823.5
49	93241.0
50	90243.5
51	87246.0
52	80281.0
53	73316.0
54	65916.5
55	58517.0
56	53757.5
57	48998.0
58	47076.5
59	45155.0
60	42425.0
61	39695.0
62	34841.0
63	29987.0
64	25787.5
65	21588.0
66	17770.5
67	13953.0
68	12022.0
69	10091.0
70	7984.5
71	5878.0
72	4857.0
73	3836.0
74	3198.0
75	1934.5
76	1309.0
77	1010.5
78	712.0
79	571.5
80	431.0
81	334.0
82	237.0
83	154.0
84	71.0
85	44.5
86	18.0
87	15.0
88	12.0
89	8.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1095821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.625876693327317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22434344938156	20.67485149838519
2	9.930243258665461	5.685238381146047
3	3.9622580836259766	3.402693339870498
4	2.1377370589064064	2.447783898040439
5	1.406185520854051	2.0126646663955157
6	0.9601995200054667	1.6491931836401137
7	0.739844807445914	1.4825094361101656
8	0.5909391419437234	1.353292080843334
9	0.4932962123518166	1.2708932893261657
>10	7.138595715646781	48.9774994062477
>50	0.363910526085899	6.299168815591403
>100	0.04762032118337573	2.631569487809225
>500	0.002574071415296549	0.5382692970735825
>1k	0.0019305535614724117	0.6488889797883965
>5k	0.0	0.0
>10k+	3.2175892691206864E-4	0.9254842397322651
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10048	0.9169380765654245	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1441	0.1314995788545757	RNA PCR Primer, Index 40 (96% over 27bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1229	0.11215335351302813	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1208	0.11023698213485597	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.125577991296024E-5	0.0	0.0	0.08797057183609366	0.0
2	9.125577991296024E-5	0.0	0.0	0.362559213594191	0.0
3	1.8251155982592049E-4	0.0	0.0	0.5294660350549953	0.0
4	1.8251155982592049E-4	0.0	0.0	0.7336052147202874	0.0
5	1.8251155982592049E-4	0.0	0.0	1.363270096119713	0.0
6	1.8251155982592049E-4	0.0	0.0	1.9223030038665074	0.0
7	1.8251155982592049E-4	0.0	0.0	2.394186641796425	0.0
8	1.8251155982592049E-4	0.0	0.0	3.1016014476816927	0.0
9	1.8251155982592049E-4	0.0	0.0	3.387232038809258	0.0
10	1.8251155982592049E-4	0.0	0.0	4.038707051607881	0.0
11	1.8251155982592049E-4	0.0	0.0	4.970428564519206	0.0
12	1.8251155982592049E-4	0.0	0.0	5.718360936685827	0.0
13	1.8251155982592049E-4	0.0	0.0	6.034653469864147	0.0
14	1.8251155982592049E-4	0.0	0.0	6.1812102524043615	0.0
15	1.8251155982592049E-4	0.0	0.0	6.40095417043477	0.0
16	1.8251155982592049E-4	0.0	0.0	6.859879487617047	0.0
17	1.8251155982592049E-4	0.0	0.0	7.398379844883426	0.0
18	1.8251155982592049E-4	0.0	0.0	7.979496651369156	0.0
19	1.8251155982592049E-4	0.0	0.0	8.359029440027157	0.0
20	1.8251155982592049E-4	0.0	0.0	8.737832182445855	0.0
21	2.737673397388807E-4	0.0	0.0	9.207708193217687	0.0
22	2.737673397388807E-4	0.0	0.0	9.709067448059491	0.0
23	3.6502311965184097E-4	0.0	0.0	10.17912597039115	0.0
24	3.6502311965184097E-4	0.0	0.0	10.58822563174095	0.0
25	3.6502311965184097E-4	0.0	0.0	10.968488466638256	0.0
26	3.6502311965184097E-4	0.0	0.0	11.314347872508375	0.0
27	3.6502311965184097E-4	0.0	0.0	11.647979003870157	0.0
28	3.6502311965184097E-4	0.0	0.0	11.990370690103584	0.0
29	3.6502311965184097E-4	0.0	0.0	12.372732407938887	0.0
30	3.6502311965184097E-4	0.0	0.0	12.809756337942055	0.0
31	4.562788995648012E-4	0.0	0.0	13.216209581674379	0.0
32	4.562788995648012E-4	0.0	0.0	13.585065444082565	0.0
33	4.562788995648012E-4	0.0	0.0	13.9592141417257	0.0
34	4.562788995648012E-4	0.0	0.0	14.334275397167968	0.0
35	6.387904593907216E-4	0.0	0.0	14.721747438678397	0.0
36	6.387904593907216E-4	0.0	0.0	15.093249718704058	0.0
37	6.387904593907216E-4	0.0	0.0	15.465573300748936	0.0
38	6.387904593907216E-4	0.0	0.0	15.84455855472746	0.0
39	6.387904593907216E-4	0.0	0.0	16.2637876076476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	20	7.033143E-4	45.0	22
GTCTCGA	25	3.8906863E-5	45.0	45
GTACGCG	20	7.033143E-4	45.0	1
CGTTTTT	3920	0.0	41.785713	1
CGTTATT	590	0.0	41.18644	1
TACGGGT	200	0.0	40.5	4
TCGTAAG	85	0.0	39.705883	1
TCGTGCG	40	3.4580262E-7	39.375	1
CTTGCGG	75	0.0	39.0	2
GTTACGG	135	0.0	38.333332	2
CGGGCAC	230	0.0	38.152172	6
CGTAAGG	225	0.0	38.0	2
TATACGG	125	0.0	37.800003	2
TATGGGC	395	0.0	37.594936	4
ACGGGTC	240	0.0	37.500004	5
ACTAACG	30	1.1398871E-4	37.500004	1
TGCACGG	115	0.0	37.173912	2
CGTATGG	110	0.0	36.81818	2
GTATACG	55	2.748493E-9	36.81818	1
TACGGGC	245	0.0	36.734695	4
>>END_MODULE