##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608940
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02284625743095	33.0	31.0	34.0	30.0	34.0
2	32.14359707031891	33.0	31.0	34.0	30.0	34.0
3	32.04334417183959	33.0	31.0	34.0	30.0	34.0
4	35.73527769566788	37.0	35.0	37.0	33.0	37.0
5	31.926639734620817	37.0	35.0	37.0	0.0	37.0
6	33.78769172660689	37.0	35.0	37.0	17.0	37.0
7	35.46743521529215	37.0	35.0	37.0	32.0	37.0
8	35.68624659243932	37.0	35.0	37.0	33.0	37.0
9	37.630398725654416	39.0	37.0	39.0	35.0	39.0
10	37.35820934739055	39.0	37.0	39.0	34.0	39.0
11	37.35365060597103	39.0	37.0	39.0	34.0	39.0
12	37.26479291884258	39.0	37.0	39.0	34.0	39.0
13	37.17378231024403	39.0	37.0	39.0	34.0	39.0
14	38.3709117482839	40.0	38.0	41.0	34.0	41.0
15	38.49719184156074	40.0	38.0	41.0	34.0	41.0
16	38.48541399809505	40.0	38.0	41.0	34.0	41.0
17	38.46782277400072	40.0	38.0	41.0	34.0	41.0
18	38.33213945544717	40.0	38.0	41.0	34.0	41.0
19	38.11695240910434	40.0	37.0	41.0	34.0	41.0
20	38.03850461457615	40.0	36.0	41.0	34.0	41.0
21	38.00185896804283	40.0	36.0	41.0	34.0	41.0
22	38.04932341445791	40.0	36.0	41.0	34.0	41.0
23	38.0500738989063	40.0	36.0	41.0	34.0	41.0
24	38.01724143593786	40.0	35.0	41.0	34.0	41.0
25	37.78047590895655	40.0	35.0	41.0	34.0	41.0
26	37.79194009261996	40.0	35.0	41.0	34.0	41.0
27	37.775766085328605	40.0	35.0	41.0	34.0	41.0
28	37.69256905442244	40.0	35.0	41.0	33.0	41.0
29	37.64853023286366	40.0	35.0	41.0	33.0	41.0
30	37.47906526094525	40.0	35.0	41.0	33.0	41.0
31	37.406209150326795	40.0	35.0	41.0	33.0	41.0
32	37.19977173448944	40.0	35.0	41.0	33.0	41.0
33	36.97462968436956	40.0	35.0	41.0	32.0	41.0
34	36.7668276020626	40.0	35.0	41.0	31.0	41.0
35	36.69742339146714	40.0	35.0	41.0	31.0	41.0
36	36.5720530758367	40.0	35.0	41.0	31.0	41.0
37	36.467407297927544	40.0	35.0	41.0	30.0	41.0
38	36.36390613196703	40.0	35.0	41.0	30.0	41.0
39	36.2437629323086	39.0	35.0	41.0	30.0	41.0
40	36.1629848589352	39.0	35.0	41.0	29.0	41.0
41	36.04980622064571	39.0	35.0	41.0	29.0	41.0
42	36.032725720103784	39.0	35.0	41.0	29.0	41.0
43	35.95667060794167	39.0	35.0	41.0	29.0	41.0
44	35.90479521791966	39.0	35.0	41.0	28.0	41.0
45	35.91172200873649	39.0	35.0	41.0	28.0	41.0
46	35.793271915131214	39.0	35.0	41.0	28.0	41.0
47	35.68496896245936	39.0	35.0	41.0	27.0	41.0
48	35.656595066837454	39.0	35.0	41.0	27.0	41.0
49	35.62005944756462	39.0	35.0	41.0	27.0	41.0
50	35.53189312575952	38.0	35.0	41.0	27.0	41.0
51	34.525055013630244	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	21.0
10	26.0
11	18.0
12	20.0
13	18.0
14	13.0
15	24.0
16	42.0
17	79.0
18	158.0
19	292.0
20	637.0
21	1094.0
22	1801.0
23	2615.0
24	3861.0
25	5716.0
26	7817.0
27	8938.0
28	8989.0
29	8933.0
30	9734.0
31	11742.0
32	14752.0
33	21314.0
34	35528.0
35	49678.0
36	45738.0
37	66563.0
38	109598.0
39	193036.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.596150688080925	19.538542385128256	22.532761848457977	15.33254507833284
2	35.76280093276842	25.363089959601933	22.83591158406411	16.03819752356554
3	30.538312477419776	25.097710776102737	29.42654448714159	14.937432259335894
4	27.930666403915	23.762111209642985	30.75081288796926	17.556409498472757
5	22.872532597628666	35.72092488586725	24.621144940388216	16.785397576115873
6	23.167799783229874	34.90343876243965	28.157289716556637	13.771471737773835
7	71.39800308733209	7.063585903373075	16.930732091831707	4.607678917463132
8	71.20915032679738	8.580155680362598	14.99556606562223	5.215127927217789
9	64.39829868295726	8.930436496206523	17.425033665057313	9.246231155778894
10	37.382172299405525	24.19548724012218	24.5206424278254	13.901698032646895
11	29.374486813150718	24.272342102670212	28.683778368969026	17.669392715210037
12	26.46270568528919	20.91158406411141	32.953657174762704	19.672053075836697
13	23.877886162840344	23.069103688376526	34.845469175945084	18.207540972838046
14	19.256084343285053	26.66108319374651	33.51857325844911	20.564259204519328
15	18.57276578973298	24.454133412158832	37.81160705488226	19.161493743225932
16	20.661641541038524	24.07265083587874	34.197293657831636	21.068413965251093
17	20.683318553552073	24.36529050481164	32.83344828718757	22.117942654448715
18	21.27894373829934	24.505698426774394	33.298190297894706	20.917167537031563
19	22.457384964035864	25.409071501297337	31.899201891812	20.234341642854798
20	23.355338785430423	25.95575918809735	32.922619634118305	17.766282392353926
21	22.683844056885736	26.650408907281502	32.68039544125858	17.98535159457418
22	20.2364765001478	23.804972575294777	33.48737149801294	22.471179426544488
23	20.479686011758137	25.9212730318258	32.91013892994384	20.68890202647223
24	21.493579006141818	24.298453049561534	32.29464315039249	21.913324793904163
25	19.542976319506028	27.20350116596052	31.17581370906822	22.077708805465235
26	18.95901073997438	26.15512201530528	32.68335139751043	22.202515847209906
27	21.69868952606168	25.4253292606825	31.90675600223339	20.969225211022433
28	17.943639767464774	26.135744079876506	34.19499458074687	21.725621571911844
29	20.050908135448484	25.796794429664665	32.865635366374356	21.286662068512495
30	21.43626629881433	27.084113377344238	31.16497520281144	20.314645121029987
31	22.833612506979343	26.402108582126317	28.875258646172036	21.889020264722305
32	23.0884816238053	27.433080434854006	29.802936249876833	19.675501691463857
33	22.006765855420895	27.049955660656224	28.902847571189277	22.0404309127336
34	21.015370972509608	26.738594935461624	30.298387361644824	21.947646730383948
35	22.861201431996584	26.18238250073899	29.28892830163891	21.667487765625513
36	22.5588727953493	29.42391697047328	27.405984169212072	20.61122606496535
37	22.651821197490722	26.40194436233455	30.737675304627714	20.208559135547016
38	20.673793805629455	28.351397510427955	28.25910598745361	22.715702696488982
39	22.124347226327718	26.589483364535095	30.09869609485335	21.187473314283835
40	22.891417873682133	26.259237363287024	30.39199264295333	20.457352120077513
41	21.631195191644498	27.225178178474067	28.385062567740665	22.758564062140767
42	22.35491181397182	25.95001149538542	29.153282753637466	22.541793937005288
43	22.040266692941834	25.87611258908924	29.891450717640488	22.19217000032844
44	20.90107399743817	25.750977107761024	29.970933096856832	23.37701579794397
45	21.416559923802016	24.948599205176208	29.01386015042533	24.620980720596446
46	23.184550201990344	26.553847669721154	28.784280881531842	21.47732124675666
47	20.880710743258778	25.794002693204586	32.15554898676388	21.16973757677275
48	21.250862153906787	25.116596052156204	30.466219988833053	23.16632180510395
49	20.90156665681348	24.329490590205932	32.89601602785168	21.872926725128913
50	20.741616579630175	23.93372089204191	31.67996846979998	23.644694058527936
51	20.00722567083785	24.150491017177387	29.101060859854826	26.741222452129932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	524.0
1	1004.5
2	1485.0
3	8784.5
4	16084.0
5	10245.0
6	4406.0
7	4157.5
8	3909.0
9	3840.5
10	3772.0
11	3707.5
12	3643.0
13	3494.0
14	3345.0
15	3207.0
16	3069.0
17	2912.0
18	2755.0
19	2667.5
20	2580.0
21	2829.5
22	3079.0
23	3281.0
24	3483.0
25	4316.5
26	5636.0
27	6122.0
28	6898.5
29	7675.0
30	9361.0
31	11047.0
32	12028.5
33	13010.0
34	14543.5
35	16077.0
36	16532.5
37	16988.0
38	18760.0
39	20532.0
40	23299.5
41	26067.0
42	29021.0
43	31975.0
44	36467.5
45	40960.0
46	49754.0
47	58548.0
48	59140.5
49	59733.0
50	56963.0
51	54193.0
52	47103.0
53	40013.0
54	35480.0
55	30947.0
56	29145.5
57	27344.0
58	25233.0
59	23122.0
60	21375.0
61	19628.0
62	18005.5
63	16383.0
64	13914.0
65	11445.0
66	9401.5
67	7358.0
68	6010.0
69	4662.0
70	3865.5
71	3069.0
72	2450.0
73	1831.0
74	1587.0
75	1062.0
76	781.0
77	599.0
78	417.0
79	328.5
80	240.0
81	163.5
82	87.0
83	57.0
84	27.0
85	24.5
86	22.0
87	13.0
88	4.0
89	4.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	608940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.0576289496096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52759586503552	25.041755775282294
2	10.695768223235733	7.285450109559756
3	4.040725847362286	4.128526247896848
4	2.091379391014999	2.8490969316819736
5	1.2994954479019392	2.2128866893175494
6	0.9062649274225048	1.8519140776920326
7	0.6668298057613524	1.5897449468012268
8	0.5199066713844009	1.4165430801949235
9	0.44523179125107715	1.3647185228699303
>10	5.597193255276062	40.63330641420628
>50	0.15684013185962814	3.4118033215654107
>100	0.04446246728708844	2.8480229160783876
>500	0.0034201897913144954	0.7631336893447552
>1k	0.004397386874547209	2.992296119013822
>5k	4.885985416163565E-4	1.6108011584948168
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9680	1.5896475843268632	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	3256	0.534699642000854	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	3072	0.504483200315302	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	2886	0.4739383190462115	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	2336	0.3836174335730942	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1645	0.27014155746050517	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1353	0.22218937826386834	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	1196	0.19640687095608764	Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1173	0.19262981574539362	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	1065	0.1748940782343088	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	707	0.11610339278089796	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCT	706	0.11593917298912866	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	689	0.11314743652905049	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.11249055736197328	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	641	0.10526488652412389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.284395835386081E-4	0.0	0.0	0.2029756626268598	0.0
2	3.284395835386081E-4	0.0	0.0	1.036555325647847	0.0
3	3.284395835386081E-4	0.0	0.0	1.3694288435642263	0.0
4	3.284395835386081E-4	0.0	0.0	1.849443294905902	0.0
5	3.284395835386081E-4	0.0	0.0	3.3890038427431275	0.0
6	3.284395835386081E-4	0.0	0.0	4.1568955890563934	0.0
7	3.284395835386081E-4	0.0	0.0	5.0141229020921605	0.0
8	3.284395835386081E-4	0.0	0.0	6.193056787203994	0.0
9	3.284395835386081E-4	0.0	0.0	6.5914540020363255	0.0
10	3.284395835386081E-4	0.0	0.0	8.096692613393767	0.0
11	3.284395835386081E-4	0.0	0.0	9.57549183827635	0.0
12	3.284395835386081E-4	0.0	0.0	11.28124281538411	0.0
13	3.284395835386081E-4	0.0	0.0	11.825467205307584	0.0
14	3.284395835386081E-4	0.0	0.0	12.061451046080073	0.0
15	3.284395835386081E-4	0.0	0.0	12.551154465136138	0.0
16	3.284395835386081E-4	0.0	0.0	13.351069070844417	0.0
17	3.284395835386081E-4	0.0	0.0	14.230301835977272	0.0
18	3.284395835386081E-4	0.0	0.0	15.17686471573554	0.0
19	3.284395835386081E-4	0.0	0.0	15.864781423457154	0.0
20	3.284395835386081E-4	0.0	0.0	16.445791046736954	0.0
21	4.926593753079121E-4	0.0	0.0	17.153085689887345	0.0
22	4.926593753079121E-4	0.0	0.0	17.881893125759518	0.0
23	4.926593753079121E-4	0.0	0.0	18.552402535553586	0.0
24	4.926593753079121E-4	0.0	0.0	19.126186487995533	0.0
25	4.926593753079121E-4	0.0	0.0	19.663842086248234	0.0
26	6.568791670772162E-4	0.0	0.0	20.134988668834367	0.0
27	6.568791670772162E-4	0.0	0.0	20.63470949518836	0.0
28	6.568791670772162E-4	0.0	0.0	21.133773442375276	0.0
29	6.568791670772162E-4	0.0	0.0	21.614280553092257	0.0
30	6.568791670772162E-4	0.0	0.0	22.183794790948205	0.0
31	6.568791670772162E-4	0.0	0.0	22.701908233980358	0.0
32	6.568791670772162E-4	0.0	0.0	23.193253850954118	0.0
33	9.853187506158243E-4	0.0	0.0	23.701842546063652	0.0
34	9.853187506158243E-4	0.0	0.0	24.203041350543568	0.0
35	0.0011495385423851282	0.0	0.0	24.71491444148849	0.0
36	0.0011495385423851282	0.0	0.0	25.206916937629323	0.0
37	0.0011495385423851282	0.0	0.0	25.69382862022531	0.0
38	0.0011495385423851282	0.0	0.0	26.194698985121686	0.0
39	0.0011495385423851282	0.0	0.0	26.728905967747234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	100	0.0	45.000004	4
CCGATCG	25	3.8886366E-5	45.000004	40
ACGGGTA	25	3.8886366E-5	45.000004	5
ACGTCGC	25	3.8886366E-5	45.000004	20
ACAACGA	40	6.8066583E-9	45.0	13
AATCACG	20	7.030671E-4	45.0	35
CGACGGT	20	7.030671E-4	45.0	28
CGCCTAA	20	7.030671E-4	45.0	30
GCCGATT	20	7.030671E-4	45.0	9
CGATCGA	45	3.8380676E-10	45.0	41
GATTACG	20	7.030671E-4	45.0	1
CGTATGG	65	0.0	44.999996	2
ACGATCG	30	2.1638134E-6	44.999996	40
CGTTATT	390	0.0	44.423077	1
CGTTTTT	3575	0.0	43.174824	1
ACTATGC	280	0.0	41.785717	8
AATGCGG	70	0.0	41.785717	2
CGTGTAC	275	0.0	41.727272	18
GCGTTAG	60	3.6379788E-12	41.249996	1
ACGGGCG	60	3.6379788E-12	41.249996	5
>>END_MODULE