##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936018.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736418
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15501386440853	33.0	31.0	34.0	30.0	34.0
2	32.28063409639634	34.0	31.0	34.0	30.0	34.0
3	32.19455119239345	33.0	31.0	34.0	30.0	34.0
4	35.832003020023954	37.0	35.0	37.0	35.0	37.0
5	32.02005247020035	37.0	35.0	37.0	0.0	37.0
6	33.86403238378204	37.0	35.0	37.0	17.0	37.0
7	35.55377652365912	37.0	35.0	37.0	32.0	37.0
8	35.82279493439867	37.0	35.0	37.0	35.0	37.0
9	37.804248402401896	39.0	38.0	39.0	35.0	39.0
10	37.45626532757211	39.0	37.0	39.0	34.0	39.0
11	37.38467962488695	39.0	37.0	39.0	35.0	39.0
12	37.268336732670846	39.0	37.0	39.0	34.0	39.0
13	37.177408754267276	39.0	37.0	39.0	34.0	39.0
14	38.29853154051096	40.0	38.0	41.0	33.0	41.0
15	38.45666048358405	40.0	38.0	41.0	34.0	41.0
16	38.471924912210184	40.0	38.0	41.0	34.0	41.0
17	38.45757436673194	40.0	38.0	41.0	34.0	41.0
18	38.35480257136572	40.0	37.0	41.0	34.0	41.0
19	38.19685966394086	40.0	37.0	41.0	34.0	41.0
20	38.194298618447675	40.0	36.0	41.0	34.0	41.0
21	38.113969240295596	40.0	36.0	41.0	34.0	41.0
22	38.18379235705808	40.0	36.0	41.0	34.0	41.0
23	38.20168165362607	40.0	36.0	41.0	34.0	41.0
24	38.167768848670185	40.0	36.0	41.0	34.0	41.0
25	37.94125618874063	40.0	36.0	41.0	34.0	41.0
26	37.95762596786064	40.0	36.0	41.0	34.0	41.0
27	37.940532415014296	40.0	36.0	41.0	34.0	41.0
28	37.89951902316348	40.0	36.0	41.0	34.0	41.0
29	37.87011860112056	40.0	36.0	41.0	34.0	41.0
30	37.695065845756076	40.0	35.0	41.0	33.0	41.0
31	37.60186605976497	40.0	35.0	41.0	33.0	41.0
32	37.459269056432625	40.0	35.0	41.0	33.0	41.0
33	37.297623089060835	40.0	35.0	41.0	33.0	41.0
34	37.09636918163326	40.0	35.0	41.0	32.0	41.0
35	37.01902587932397	40.0	35.0	41.0	32.0	41.0
36	36.90254855258834	40.0	35.0	41.0	32.0	41.0
37	36.8236042573647	40.0	35.0	41.0	31.0	41.0
38	36.7160661472153	40.0	35.0	41.0	31.0	41.0
39	36.64117525644403	40.0	35.0	41.0	31.0	41.0
40	36.5728988156183	40.0	35.0	41.0	31.0	41.0
41	36.41142666257479	39.0	35.0	41.0	30.0	41.0
42	36.41991097447374	39.0	35.0	41.0	30.0	41.0
43	36.355139336626756	39.0	35.0	41.0	30.0	41.0
44	36.319663560640834	39.0	35.0	41.0	30.0	41.0
45	36.320228457207726	39.0	35.0	41.0	30.0	41.0
46	36.22252036207697	39.0	35.0	41.0	30.0	41.0
47	36.113522483154945	39.0	35.0	41.0	30.0	41.0
48	36.08557096648914	39.0	35.0	41.0	30.0	41.0
49	36.05154545380477	39.0	35.0	41.0	30.0	41.0
50	35.951857504841	39.0	35.0	41.0	30.0	41.0
51	34.989865809906874	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	12.0
9	29.0
10	41.0
11	14.0
12	23.0
13	19.0
14	19.0
15	40.0
16	66.0
17	93.0
18	205.0
19	357.0
20	708.0
21	1273.0
22	1887.0
23	2882.0
24	4054.0
25	5887.0
26	7747.0
27	8674.0
28	8667.0
29	9043.0
30	10262.0
31	12605.0
32	17399.0
33	25867.0
34	45954.0
35	56600.0
36	52910.0
37	81705.0
38	135120.0
39	246020.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95753227107431	19.50509085872426	23.651784720091033	13.885592150110398
2	34.61444451384948	24.31513080886127	24.79013277785171	16.28029189943755
3	30.780344858490693	24.622429109554627	28.653835185994907	15.943390845959767
4	27.27622084196747	24.46205823323167	30.198066858767707	18.06365406603315
5	23.070049890143913	36.191402165617895	24.024127601443745	16.714420342794444
6	24.23745753091315	34.087705623708274	27.381595778484503	14.293241066894074
7	77.20370767689003	5.816261959919503	12.52644557846223	4.453584784728239
8	78.98557612660201	6.263698062784995	10.717418639957197	4.033307170655796
9	73.11418243443262	7.407342025860313	12.378296022096148	7.100179517610922
10	40.889005972151686	26.109627955862024	19.74340659788327	13.257959474103023
11	30.956875035645513	24.669956464942462	26.463910442167354	17.909258057244664
12	27.59709838705735	22.48600658864938	30.467071690262866	19.4498233340304
13	25.53305323878558	23.114589811764514	32.452221428590825	18.900135520859077
14	20.608404465941895	26.412037728572628	32.882411891072735	20.09714591441274
15	19.24070297032392	25.196831147527625	36.153380281307626	19.40908560084083
16	23.90734609963363	24.545299001382368	31.499094264398753	20.04826063458525
17	24.170104478706385	25.14984696191565	30.02126509672496	20.658783462653005
18	24.36917620155944	24.18286896843912	31.575816995239116	19.87213783476232
19	24.16562332805553	26.32730324353832	29.412643362872714	20.094430065533434
20	24.684893633778643	27.035053461485187	29.92213661263033	18.35791629210584
21	24.011498904154976	26.811674891162358	31.10271612046419	18.07411008421847
22	22.963045444299297	23.73339597891415	32.27324698744463	21.030311589341924
23	22.024991241387365	26.01973335795703	31.341031859623204	20.614243541032405
24	22.088677897607063	25.585740707044096	31.669377989131174	20.656203406217664
25	22.528509623610503	26.7822079308219	29.465330831131233	21.223951614436366
26	20.95128038695415	26.604591414115355	30.283072928689958	22.161055270240542
27	21.17031359907009	26.80732953295547	31.13476313723999	20.887593730734448
28	19.756985842279793	26.499623854930217	31.740397437324997	22.002992865464993
29	21.419085356414428	25.762678261530812	31.535079262049543	21.283157120005214
30	22.530818095157912	26.515918948206046	30.752778992365748	20.20048396427029
31	23.938578361745638	25.978724039879523	29.019795822481253	21.062901775893582
32	25.6107265167337	26.166117612551567	28.684931655662954	19.538224215051777
33	24.892112903269613	26.118318672275798	27.552694257880717	21.436874166573876
34	21.89164306141349	26.601739772792083	30.510253687443818	20.99636347835061
35	22.76207262723073	27.644489950001223	28.8558943426152	20.737543080152847
36	23.914678891607753	29.041251028627762	27.21389211018742	19.83017796957706
37	23.20611391899709	28.99277312613217	27.843833257742208	19.957279697128534
38	23.379520869940713	27.638107705134857	26.87210252872689	22.110268896197542
39	22.568704187024217	27.24281590075202	27.605381726139232	22.583098186084534
40	23.4702302225095	25.638292382858648	29.06447153654582	21.827005858086032
41	21.519707557392675	27.54400354146694	28.162266538840715	22.77402236229967
42	22.2417159819559	26.577976095098165	29.198227093851592	21.982080829094347
43	22.17857249551206	26.369534693611506	28.85426483328762	22.597627977588814
44	22.044137975986462	25.791602052095413	29.019660030037286	23.144599941880834
45	22.049841258633005	25.042435138739137	28.33662403689209	24.571099565735764
46	22.961415934971715	25.99746339714673	28.193634593396684	22.84748607448487
47	20.901037182687006	26.75206200826161	30.603135719116047	21.743765089935334
48	21.446108052763513	27.529066372630762	29.03459719887347	21.99022837573226
49	22.751616609045406	25.411790586324614	30.13288648566439	21.703706318965587
50	21.4281834501601	25.984155737638137	29.66290883710067	22.9247519751011
51	20.589257731342798	25.936628382250298	28.47200910352544	25.002104782881464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	468.0
1	922.5
2	1377.0
3	6456.5
4	11536.0
5	7494.0
6	3452.0
7	3323.5
8	3195.0
9	3169.0
10	3143.0
11	3112.5
12	3082.0
13	3028.0
14	2974.0
15	2915.0
16	2856.0
17	2800.0
18	2744.0
19	2823.0
20	2902.0
21	3056.0
22	3210.0
23	3489.0
24	3768.0
25	4389.0
26	6368.5
27	7727.0
28	8883.5
29	10040.0
30	10977.5
31	11915.0
32	14529.5
33	17144.0
34	20179.0
35	23214.0
36	24281.0
37	25348.0
38	26981.0
39	28614.0
40	31507.5
41	34401.0
42	37066.0
43	39731.0
44	43473.0
45	47215.0
46	55346.0
47	63477.0
48	62935.0
49	62393.0
50	61753.0
51	61113.0
52	56396.0
53	51679.0
54	46197.0
55	40715.0
56	38006.0
57	35297.0
58	33637.0
59	31977.0
60	29275.5
61	26574.0
62	24108.0
63	21642.0
64	18477.0
65	15312.0
66	13124.5
67	10937.0
68	9430.5
69	7924.0
70	6426.0
71	4928.0
72	3959.0
73	2990.0
74	2527.0
75	1680.5
76	1297.0
77	964.5
78	632.0
79	393.0
80	154.0
81	118.5
82	83.0
83	94.0
84	105.0
85	65.5
86	26.0
87	20.5
88	15.0
89	12.0
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	736418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.64104131303105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48287159540313	23.25074575950306
2	9.529689010544638	6.030585673659617
3	3.73588717275615	3.546220811220003
4	2.1035502027436075	2.662340754761813
5	1.396480040964616	2.209304133449235
6	0.9916856281187807	1.882677955730729
7	0.722230010095012	1.5996476710848972
8	0.604147508583766	1.5292685022610975
9	0.5271446786856424	1.5011465900613201
>10	6.65364941363075	47.43269882703626
>50	0.22004880840524035	4.108055928038481
>100	0.02739738125767045	1.5427741760613367
>500	0.003044153473074494	0.6525004586945111
>1k	0.0017395162703282825	1.164373446114077
>5k	4.3487906758207063E-4	0.8876593123235537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6451	0.8759970560198149	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	2418	0.32834612950796965	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	2327	0.3159890171071321	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	2248	0.30526141403387747	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1469	0.1994791001849493	Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.10795499295237217	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	759	0.10306646496962323	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10265908763772749	0.0
2	0.0	0.0	0.0	0.5024320426714176	0.0
3	0.0	0.0	0.0	0.6852086722486413	0.0
4	0.0	0.0	0.0	0.948917598429153	0.0
5	0.0	0.0	0.0	1.8314326917592998	0.0
6	0.0	0.0	0.0	2.347851356159138	0.0
7	0.0	0.0	0.0	2.8938727733434	0.0
8	0.0	0.0	0.0	3.6530883275531014	0.0
9	0.0	0.0	0.0	3.970842646431782	0.0
10	0.0	0.0	0.0	4.954251525628108	0.0
11	0.0	0.0	0.0	5.922044273768431	0.0
12	0.0	0.0	0.0	7.020333560559356	0.0
13	0.0	0.0	0.0	7.360629425136268	0.0
14	0.0	0.0	0.0	7.538789111618673	0.0
15	0.0	0.0	0.0	7.837125111010323	0.0
16	0.0	0.0	0.0	8.291350836074077	0.0
17	0.0	0.0	0.0	8.816867594219588	0.0
18	0.0	0.0	0.0	9.347272880347846	0.0
19	0.0	0.0	0.0	9.785882474355597	0.0
20	0.0	0.0	0.0	10.157682185932446	0.0
21	0.0	0.0	0.0	10.609463647004826	0.0
22	0.0	0.0	0.0	11.130906631831378	0.0
23	0.0	0.0	0.0	11.5977610541839	0.0
24	0.0	0.0	0.0	11.983683179933136	0.0
25	0.0	0.0	0.0	12.333756100475545	0.0
26	0.0	0.0	0.0	12.671200323729186	0.0
27	0.0	0.0	0.0	13.049110695284472	0.0
28	0.0	0.0	0.0	13.393072955848444	0.0
29	0.0	0.0	0.0	13.778859289153715	0.0
30	1.35792443965248E-4	0.0	0.0	14.227381731570928	0.0
31	1.35792443965248E-4	0.0	0.0	14.602033084471048	0.0
32	1.35792443965248E-4	0.0	0.0	14.95373551434104	0.0
33	1.35792443965248E-4	0.0	0.0	15.311820189077398	0.0
34	1.35792443965248E-4	0.0	0.0	15.681175636662873	0.0
35	2.71584887930496E-4	0.0	0.0	16.082034931248284	0.0
36	2.71584887930496E-4	0.0	0.0	16.441613322868264	0.0
37	2.71584887930496E-4	0.0	0.0	16.821017411307164	0.0
38	2.71584887930496E-4	0.0	0.0	17.2389865538322	0.0
39	2.71584887930496E-4	0.0	0.0	17.876124700917142	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAC	20	7.031634E-4	45.0	15
ATTGTCG	20	7.031634E-4	45.0	2
CACGATA	25	3.889435E-5	45.0	27
GGACGTA	20	7.031634E-4	45.0	8
CGTGTAT	25	3.889435E-5	45.0	20
TATAGCG	25	3.889435E-5	45.0	1
CGATCGA	35	1.2111377E-7	45.0	41
CGGTCTA	30	2.1644355E-6	44.999996	31
CCCGCTA	30	2.1644355E-6	44.999996	34
AATCGTT	30	2.1644355E-6	44.999996	22
CGTTTTT	2885	0.0	42.58232	1
TCACGAC	50	1.0804797E-9	40.5	25
GCCGATC	50	1.0804797E-9	40.5	39
GCGATAA	50	1.0804797E-9	40.5	9
CGCCGAT	40	3.4559707E-7	39.375	38
TAATACG	75	0.0	39.000004	4
CGTTATT	425	0.0	38.647057	1
CGGGTAT	70	0.0	38.571426	6
CGACGGT	35	6.2452327E-6	38.571426	28
CGTTGAT	35	6.2452327E-6	38.571426	25
>>END_MODULE