FastQCFastQC Report
Sat 14 Jan 2017
SRR2936017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160651
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421653.6328749985999234No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC58600.5048890665669525No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT58140.5009257735529458No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT41530.3578164323297873No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC37370.3219744781161607No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC25450.21927349392711504No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC22230.19153044282906748No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG20870.1798128808746126TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG19900.17145550212768523No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT19020.16387355027480266No Hit
CGTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT18870.16258117211806133No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGT18660.16077184269862346No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT18460.1590486718229683TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG17440.15026050035712718No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC16800.14474635355503077TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC15680.13509659665136203TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC14320.12337903469690717No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC11720.1009778133133905No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT11620.10011622787556293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG502.1827873E-1145.0000042
CGTTATT30550.044.0425531
CGTTTTT236000.043.7701261
GTTATTT33300.040.810812
CGTTTCT7000.039.8571431
CGTTCTG5350.038.691591
GTTCGCG356.2483414E-638.571431
GCGTAAG1000.038.2500041
GTTTTTT269900.038.2391622
CGTAAGG1600.037.968752
CGTTTTC5100.037.9411771
ACTACGG301.1399288E-437.4999962
ACGTAAG301.1399288E-437.4999961
CCCGCTA301.1399288E-437.49999624
ACGGGTG2250.037.05
TAACGGG3300.036.818183
TGTTACG552.748493E-936.818181
TACGAAT800.036.562512
AAGGGCG3700.036.4864855
TACGGGT1800.036.2500044
Produced by FastQC (version 0.11.5)