##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	760177
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84188682372658	33.0	31.0	34.0	30.0	34.0
2	31.978018277322256	33.0	31.0	34.0	30.0	34.0
3	31.863551514976116	33.0	31.0	34.0	30.0	34.0
4	35.58972055192409	37.0	35.0	37.0	33.0	37.0
5	31.997855762539515	37.0	35.0	37.0	0.0	37.0
6	33.77639549736443	37.0	35.0	37.0	17.0	37.0
7	35.4275188541616	37.0	35.0	37.0	32.0	37.0
8	35.68158863001643	37.0	35.0	37.0	33.0	37.0
9	37.62899956194413	39.0	37.0	39.0	35.0	39.0
10	37.21408566689074	39.0	37.0	39.0	34.0	39.0
11	37.24140430452381	39.0	37.0	39.0	34.0	39.0
12	37.15375892719722	39.0	37.0	39.0	34.0	39.0
13	37.0401551217677	39.0	37.0	39.0	33.0	39.0
14	37.96449248004083	40.0	37.0	41.0	33.0	41.0
15	38.194925655472346	40.0	38.0	41.0	33.0	41.0
16	38.22122347821626	40.0	37.0	41.0	33.0	41.0
17	38.27987823888384	40.0	37.0	41.0	34.0	41.0
18	38.15549799586149	40.0	37.0	41.0	34.0	41.0
19	37.92847718360329	40.0	37.0	41.0	34.0	41.0
20	37.860562737362486	40.0	35.0	41.0	34.0	41.0
21	37.77379215630044	39.0	35.0	41.0	33.0	41.0
22	37.87336238797017	40.0	35.0	41.0	34.0	41.0
23	37.896283365584594	40.0	35.0	41.0	34.0	41.0
24	37.832153564235696	40.0	35.0	41.0	34.0	41.0
25	37.573358573069164	39.0	35.0	41.0	33.0	41.0
26	37.619066349021345	39.0	35.0	41.0	33.0	41.0
27	37.6138412501299	39.0	35.0	41.0	33.0	41.0
28	37.55431695513019	39.0	35.0	41.0	33.0	41.0
29	37.513251519054116	39.0	35.0	41.0	33.0	41.0
30	37.322122347821626	39.0	35.0	41.0	33.0	41.0
31	37.19530056815715	39.0	35.0	41.0	33.0	41.0
32	37.013308742569166	39.0	35.0	41.0	32.0	41.0
33	36.79138674282437	39.0	35.0	41.0	31.0	41.0
34	36.53043567484941	39.0	35.0	41.0	31.0	41.0
35	36.41953255623361	39.0	35.0	41.0	30.0	41.0
36	36.26323474664453	39.0	35.0	41.0	30.0	41.0
37	36.15734756510655	39.0	35.0	41.0	30.0	41.0
38	36.02778694961831	39.0	35.0	41.0	29.0	41.0
39	35.93812230572617	39.0	35.0	41.0	29.0	41.0
40	35.80635562507153	39.0	35.0	41.0	27.0	41.0
41	35.61382151788334	39.0	35.0	41.0	26.0	41.0
42	35.635354660822415	39.0	35.0	41.0	27.0	41.0
43	35.58625293846038	39.0	35.0	41.0	26.0	41.0
44	35.54786056405285	39.0	35.0	41.0	26.0	41.0
45	35.55070332304187	39.0	35.0	41.0	26.0	41.0
46	35.4339068401175	39.0	35.0	40.0	26.0	41.0
47	35.350359192661706	38.0	35.0	40.0	26.0	41.0
48	35.314835886905286	38.0	35.0	40.0	26.0	41.0
49	35.32118572385116	38.0	35.0	40.0	26.0	41.0
50	35.19876686613775	38.0	35.0	40.0	25.0	41.0
51	34.22945314051859	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	23.0
10	26.0
11	12.0
12	19.0
13	12.0
14	22.0
15	25.0
16	58.0
17	109.0
18	239.0
19	406.0
20	718.0
21	1301.0
22	2043.0
23	3223.0
24	5193.0
25	8413.0
26	11403.0
27	12244.0
28	12319.0
29	12216.0
30	13736.0
31	16442.0
32	21428.0
33	30564.0
34	49433.0
35	56909.0
36	57619.0
37	84868.0
38	141613.0
39	217419.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.623504789016245	20.442870541992196	22.6428844861131	18.290740182878462
2	36.01134998822643	23.375608575371263	23.738418815617944	16.87462262078437
3	28.796977546018887	23.017534074301118	32.82261894269361	15.362869436986387
4	26.45318129856599	23.00819414425851	33.452209156551696	17.086415400623803
5	20.213844933482598	33.86408691659969	29.29396706293403	16.62810108698369
6	21.483286129414598	30.344117225330418	33.396958866158805	14.775637779096185
7	71.77999334365549	5.199973164144666	17.74823495054441	5.27179854165543
8	72.59861847964356	4.831900991479616	16.39184032139883	6.177640207477995
9	67.74698524159504	5.7223515049784455	19.214472418923485	7.316190834503017
10	35.66616722158129	20.629142949602528	27.515170808903715	16.189519019912467
11	27.044359405769974	23.29996829685718	30.966340733802785	18.689331563570065
12	24.88367840647639	19.452443312544315	35.68405779180375	19.979820489175548
13	23.017270977680198	20.4570777595218	37.871048453189196	18.654602809608814
14	18.32757370980706	23.734472366304164	37.800275462162105	20.137678461726676
15	16.70847710467431	22.901903109407414	41.46626377804117	18.92335600787711
16	20.560211634921867	23.366005548707736	35.73667711598746	20.33710570038294
17	20.645981133341316	22.977938032852872	33.65584594114265	22.720234892663154
18	21.314641195405805	22.281389728971014	36.752493169353976	19.651475906269198
19	20.636772751609165	24.644786674682344	33.43037213701546	21.288068436693035
20	19.543474743382134	27.45281690974602	33.706228944048554	19.29747940282329
21	19.157248903873704	26.49317198494561	36.31299026410954	18.03658884707114
22	17.762179071453097	22.302569006954958	36.2436643044975	23.691587617094438
23	17.573801890875416	24.209493315372605	35.97859445892206	22.238110334829912
24	20.122681954334322	24.01046072164772	34.3627865615508	21.504070762467162
25	19.781314088692504	24.85039668393019	32.28629648095115	23.08199274642616
26	16.962496892171167	23.8548390703744	33.65663523100541	25.526028806449023
27	18.892573703229644	23.247612069294387	34.41264337121486	23.447170856261106
28	15.922870594611519	23.06068192013176	34.65587619725406	26.36057128800266
29	17.77770177208729	22.68169123769859	34.03930926613144	25.501297724082683
30	20.112421186118496	22.15010451513266	32.48388204326098	25.253592255487867
31	18.37230013536321	23.970075390336724	31.492139330708508	26.16548514359156
32	19.69422910716846	24.40773661923473	30.66772606906023	25.230308204536573
33	19.058719219339707	23.91640367966934	29.726234811103204	27.29864228988775
34	16.075466634744277	24.408920554028864	32.648843624576905	26.866769186649954
35	16.189913664843846	26.01183671697512	30.085361698657024	27.712887919524004
36	18.629477082311094	26.860586416058364	27.571605034090744	26.938331467539793
37	16.340931125251092	27.793263937214626	31.210757494636116	24.655047442898166
38	17.60366335734967	27.46728722389654	29.624284870497263	25.304764548256525
39	18.39663657279818	24.990232537948398	31.018302316434198	25.59482857281922
40	20.386699413426086	24.512185977739396	30.701928629779644	24.399185979054877
41	17.764283844420444	26.960694680317875	27.86272144513712	27.412300030124566
42	18.41663191598799	25.97658176977204	30.83663409968994	24.77015221455003
43	19.470728527698157	23.806429292125387	31.883890199256225	24.838951980920232
44	19.693439817305705	22.352294268308565	30.6694361970962	27.284829717289526
45	19.253279170508975	21.730333856457115	29.672036907193984	29.34435006583993
46	20.49956786380014	23.27642114928497	29.891064844108673	26.332946142806215
47	16.55930132061349	24.3228879589885	33.177141639381354	25.940669081016658
48	17.297945083842315	24.947742433670054	31.082760988559244	26.671551493928387
49	19.790259373803732	22.11721743751784	32.595698107151364	25.496825081527064
50	18.415053336262478	21.57497530180471	32.1565898468383	27.85338151509451
51	17.16271342069018	22.87914525169796	29.09953866007522	30.858602667536637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	470.0
1	772.5
2	1075.0
3	11310.0
4	21545.0
5	13488.0
6	5431.0
7	5189.0
8	4947.0
9	5094.5
10	5242.0
11	5081.0
12	4920.0
13	4808.5
14	4697.0
15	4427.0
16	4157.0
17	3950.5
18	3744.0
19	3476.5
20	3209.0
21	3187.0
22	3165.0
23	3235.5
24	3306.0
25	3645.0
26	4782.0
27	5580.0
28	6433.0
29	7286.0
30	8068.5
31	8851.0
32	10077.0
33	11303.0
34	13531.5
35	15760.0
36	16830.0
37	17900.0
38	20320.0
39	22740.0
40	26073.5
41	29407.0
42	33757.5
43	38108.0
44	44844.0
45	51580.0
46	64859.5
47	78139.0
48	84753.0
49	91367.0
50	89038.5
51	86710.0
52	72541.0
53	58372.0
54	51438.5
55	44505.0
56	40000.0
57	35495.0
58	32043.5
59	28592.0
60	23752.5
61	18913.0
62	16062.0
63	13211.0
64	11519.0
65	9827.0
66	7794.5
67	5762.0
68	4873.5
69	3985.0
70	3324.5
71	2664.0
72	2252.5
73	1841.0
74	1520.0
75	868.0
76	537.0
77	463.0
78	389.0
79	270.0
80	151.0
81	113.0
82	75.0
83	46.5
84	18.0
85	15.0
86	12.0
87	9.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	760177.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.725889814267106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73537968893807	27.440069197437587
2	11.509648078366244	8.684234304108722
3	4.561146619068134	5.162199443330441
4	2.293672005705246	3.461232694292206
5	1.4694393419503935	2.7717953351584854
6	0.9695400629991792	2.194605695233477
7	0.7041968366665323	1.8596516587345933
8	0.5377462591308027	1.6229564896002389
9	0.44380900227862724	1.506878056668898
>10	4.633676180779563	34.32818242447148
>50	0.08898689877342102	2.2403732405583154
>100	0.04607621546698975	3.33256155040801
>500	0.002462087849381096	0.5571740374343271
>1k	0.0038689951918845795	2.996187131523483
>5k	0.0	0.0
>10k+	3.517268356258708E-4	1.8418987410397465
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13881	1.8260220974851908	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	3991	0.525009307042965	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2789	0.3668882378709169	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	2351	0.30927007788975464	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	2075	0.2729627442029948	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	2052	0.2699371330624315	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	2033	0.2674377151637053	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1730	0.2275785770945451	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	1691	0.22244819298663338	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1613	0.21218742477080993	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1180	0.15522700634194403	No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1075	0.1414144337437202	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.261932418370985E-4	0.0	0.0	0.3211094258310893	0.0
2	5.261932418370985E-4	0.0	0.0	1.6410651729794508	0.0
3	5.261932418370985E-4	0.0	0.0	2.200671685673205	0.0
4	5.261932418370985E-4	0.0	0.0	2.90708611283951	0.0
5	5.261932418370985E-4	0.0	0.0	4.90740972168324	0.0
6	5.261932418370985E-4	0.0	0.0	6.321685607430901	0.0
7	5.261932418370985E-4	0.0	0.0	7.783845078185738	0.0
8	5.261932418370985E-4	0.0	0.0	9.949130268345398	0.0
9	5.261932418370985E-4	0.0	0.0	10.733815940234972	0.0
10	5.261932418370985E-4	0.0	0.0	12.588515569400284	0.0
11	5.261932418370985E-4	0.0	0.0	14.883638941983248	0.0
12	5.261932418370985E-4	0.0	0.0	16.80029782537488	0.0
13	5.261932418370985E-4	0.0	0.0	17.523945081211348	0.0
14	5.261932418370985E-4	0.0	0.0	17.818350200019207	0.0
15	5.261932418370985E-4	0.0	0.0	18.299027726437394	0.0
16	5.261932418370985E-4	0.0	0.0	19.245649368502335	0.0
17	5.261932418370985E-4	0.0	0.0	20.346445630425546	0.0
18	5.261932418370985E-4	0.0	0.0	21.580500330844	0.0
19	5.261932418370985E-4	0.0	0.0	22.363212778076687	0.0
20	5.261932418370985E-4	0.0	0.0	23.128429300018286	0.0
21	5.261932418370985E-4	0.0	0.0	24.098466541344976	0.0
22	5.261932418370985E-4	0.0	0.0	25.09205093024388	0.0
23	5.261932418370985E-4	0.0	0.0	26.069717973577205	0.0
24	5.261932418370985E-4	0.0	0.0	26.85624532181321	0.0
25	5.261932418370985E-4	0.0	0.0	27.53437686223077	0.0
26	5.261932418370985E-4	0.0	0.0	28.181068356448563	0.0
27	5.261932418370985E-4	0.0	0.0	28.898927486624824	0.0
28	5.261932418370985E-4	0.0	0.0	29.55653749061074	0.0
29	5.261932418370985E-4	0.0	0.0	30.25703224380638	0.0
30	5.261932418370985E-4	0.0	0.0	31.071316285549287	0.0
31	6.577415522963731E-4	0.0	0.0	31.715245265247436	0.0
32	6.577415522963731E-4	0.0	0.0	32.332206841301435	0.0
33	6.577415522963731E-4	0.0	0.0	32.939039197450064	0.0
34	6.577415522963731E-4	0.0	0.0	33.5891509477398	0.0
35	6.577415522963731E-4	0.0	0.0	34.2564955266997	0.0
36	6.577415522963731E-4	0.0	0.0	34.87858748686161	0.0
37	6.577415522963731E-4	0.0	0.0	35.508835442271995	0.0
38	6.577415522963731E-4	0.0	0.0	36.197490847526296	0.0
39	7.892898627556477E-4	0.0	0.0	37.244352302161204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGTG	45	3.8380676E-10	45.000004	1
TCACGAC	50	2.1827873E-11	45.0	25
TTAGGCG	25	3.8895545E-5	45.0	1
GCGACTT	25	3.8895545E-5	45.0	1
TAACGCC	40	6.8102963E-9	45.0	12
GCGTTAC	20	7.031779E-4	45.0	26
CGTAGTC	20	7.031779E-4	45.0	40
CCTCACG	20	7.031779E-4	45.0	10
GACGGTC	50	2.1827873E-11	45.0	29
CGAATAT	70	0.0	45.0	14
TCGCGTG	20	7.031779E-4	45.0	41
TAGCACG	30	2.1645283E-6	44.999996	1
TTACGCG	30	2.1645283E-6	44.999996	1
CGTTTTT	5560	0.0	43.66457	1
CGTTATT	620	0.0	43.185482	1
CTCACGA	55	6.002665E-11	40.90909	24
CGACGGT	55	6.002665E-11	40.90909	28
CACGACG	55	6.002665E-11	40.90909	26
CGGTCTA	55	6.002665E-11	40.90909	31
ATAACGC	55	6.002665E-11	40.90909	11
>>END_MODULE