FastQCFastQC Report
Sat 14 Jan 2017
SRR2936013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041074
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207051.98881155422189No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.2355260048757341No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC19140.1838486025008789No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT14580.14004768152888267Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG14130.13572522222243566Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT13490.1295777245421555No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC12950.12439077337441912Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC12500.12006831406797211Illumina PCR Primer Index 11 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC10970.10537195242605232Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT10910.10479562451852606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90050.044.0005571
CGTTATT12150.042.592591
TCGATAG451.9279469E-840.01
TATTACG451.9279469E-840.01
TGCGTAG451.9279469E-840.01
GCGTAAG1300.039.807691
CTGACGG850.039.7058832
ACGTGCG403.457808E-739.3751
TAACGGG2650.039.0566023
GGTACGA356.247723E-638.5714268
CACGTAG356.247723E-638.5714261
TGTAACG356.247723E-638.5714261
TACGGGA5350.038.2710274
ATTGTCG301.1398478E-437.5000041
TAGCCGT301.1398478E-437.50000444
GTTTTTT109200.037.1909332
ATTACGG850.037.0588232
ACGGGAT6150.036.9512185
TCACGAC552.748493E-936.8181825
CGGTCTA552.748493E-936.8181831
Produced by FastQC (version 0.11.5)