##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936013.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041074
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07619919429359	33.0	31.0	34.0	30.0	34.0
2	32.232290884221484	34.0	31.0	34.0	30.0	34.0
3	32.06571482910917	33.0	31.0	34.0	30.0	34.0
4	35.76801360902299	37.0	35.0	37.0	35.0	37.0
5	32.073113918895295	37.0	35.0	37.0	0.0	37.0
6	33.8844318463433	37.0	35.0	37.0	17.0	37.0
7	35.559440539289234	37.0	35.0	37.0	32.0	37.0
8	35.827436858475	37.0	35.0	37.0	35.0	37.0
9	37.81669026409266	39.0	38.0	39.0	35.0	39.0
10	37.440614211861984	39.0	37.0	39.0	34.0	39.0
11	37.3921892199786	39.0	37.0	39.0	34.0	39.0
12	37.396931438111025	39.0	37.0	39.0	35.0	39.0
13	37.38567863571658	39.0	37.0	39.0	35.0	39.0
14	38.65091434422529	40.0	38.0	41.0	35.0	41.0
15	38.78376657182871	40.0	38.0	41.0	35.0	41.0
16	38.70108368857545	40.0	38.0	41.0	35.0	41.0
17	38.6991040022131	40.0	38.0	41.0	35.0	41.0
18	38.49341257201697	40.0	38.0	41.0	35.0	41.0
19	38.2350716663753	40.0	37.0	41.0	34.0	41.0
20	38.184440299152605	40.0	36.0	41.0	34.0	41.0
21	38.07450094805941	40.0	36.0	41.0	34.0	41.0
22	38.12360216468762	40.0	36.0	41.0	34.0	41.0
23	38.136434105548695	40.0	36.0	41.0	34.0	41.0
24	38.076256827084336	40.0	35.0	41.0	34.0	41.0
25	37.80444041441819	40.0	35.0	41.0	34.0	41.0
26	37.86404520716107	40.0	35.0	41.0	34.0	41.0
27	37.85145628456767	40.0	35.0	41.0	34.0	41.0
28	37.780926235791114	40.0	35.0	41.0	34.0	41.0
29	37.72796170108945	40.0	35.0	41.0	33.0	41.0
30	37.502377352618545	40.0	35.0	41.0	33.0	41.0
31	37.38559891035604	40.0	35.0	41.0	33.0	41.0
32	37.140401162645496	40.0	35.0	41.0	33.0	41.0
33	36.823134570645315	40.0	35.0	41.0	31.0	41.0
34	36.50521960974916	40.0	35.0	41.0	30.0	41.0
35	36.33347773549239	40.0	35.0	41.0	30.0	41.0
36	36.15493327083377	40.0	35.0	41.0	29.0	41.0
37	36.07209285795246	40.0	35.0	41.0	28.0	41.0
38	35.94234223503805	39.0	35.0	41.0	27.0	41.0
39	35.83647752225106	39.0	35.0	41.0	26.0	41.0
40	35.747105393084446	39.0	35.0	41.0	26.0	41.0
41	35.599011213419985	39.0	35.0	41.0	25.0	41.0
42	35.54139283086505	39.0	35.0	41.0	24.0	41.0
43	35.43129691069031	39.0	35.0	41.0	24.0	41.0
44	35.34832298184375	39.0	35.0	41.0	23.0	41.0
45	35.296709936085236	39.0	35.0	41.0	23.0	41.0
46	35.14478509692875	39.0	35.0	41.0	23.0	41.0
47	35.06463325373605	38.0	35.0	41.0	23.0	41.0
48	35.000055711697726	38.0	35.0	41.0	23.0	41.0
49	34.955538223027375	38.0	35.0	41.0	23.0	41.0
50	34.842127456837844	38.0	35.0	40.0	23.0	41.0
51	33.85935870072637	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	21.0
9	51.0
10	48.0
11	41.0
12	23.0
13	26.0
14	22.0
15	47.0
16	89.0
17	181.0
18	347.0
19	657.0
20	1174.0
21	1783.0
22	2799.0
23	4386.0
24	7287.0
25	12256.0
26	17571.0
27	19408.0
28	18366.0
29	16823.0
30	17175.0
31	19566.0
32	23987.0
33	33111.0
34	55004.0
35	75031.0
36	80082.0
37	119205.0
38	192986.0
39	321277.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.8995863886717	19.95544985274822	23.32840893154569	19.816554827034388
2	39.095875989603044	21.731116135836643	22.90682506719023	16.26618280737008
3	29.275248445355466	21.51816297400569	33.35497764808265	15.851610932556188
4	25.521912947590664	23.622432219035343	32.96758923957375	17.88806559380025
5	21.06459291078252	35.08482586252274	28.01577985810807	15.834801368586671
6	23.57613387713073	30.7942567002922	31.276066830984156	14.353542591592912
7	74.02153929499728	5.44860403775332	16.06235483740829	4.467501829841106
8	75.81507174321902	4.465580736816019	15.774671156901432	3.9446763630635284
9	70.22478709486549	6.800381144856177	17.65638177497469	5.318449985303638
10	36.08369818091701	26.285835589016727	24.098767234605802	13.531698995460456
11	25.477055425454864	23.67900840862417	32.24717935516592	18.596756810755046
12	22.79472928917637	21.060558615429837	36.103677548377924	20.041034547015872
13	21.525943400757296	21.636982577607355	37.79885003371518	19.038223987920166
14	19.23023723577767	24.04814643339474	37.29590787974726	19.425708451080325
15	18.39360122335204	23.52522491196591	38.29650918186411	19.784664682817937
16	21.573778617081977	23.69149551328724	36.072747950674014	18.661977918956772
17	21.587034158955078	23.886198291379866	34.23791200241289	20.288855547252165
18	21.755706126557765	23.827797063417204	35.16320645794631	19.253290352078718
19	22.013901029129535	25.552170162735788	33.125310976933434	19.308617831201243
20	22.838722319450873	25.575607497641855	33.140679721134134	18.44499046177313
21	22.642770830891944	24.653386790948577	34.30111596293827	18.402726415221203
22	22.079986629192547	22.718461896080395	33.75600581706968	21.44554565765738
23	21.117230859669917	23.973319860067583	34.15127070698144	20.758178573281054
24	20.760772048864922	23.833560342492465	34.768517895942075	20.637149712700538
25	20.90379742458269	25.05038066458292	33.25488870147559	20.790933209358798
26	20.609101754534258	25.503854673154834	33.41779739000302	20.469246182307884
27	20.577019501015297	24.290780482463305	34.42118427700625	20.711015739515155
28	19.32110493586431	24.769805028268884	34.99760823918376	20.91148179668304
29	20.52111569398525	23.599283048083038	34.348663015309185	21.530938242622522
30	21.331048513362163	23.81982452736309	34.09911303135031	20.75001392792443
31	22.03340012333417	24.39864985582197	32.73821073237829	20.829739288465564
32	22.080659011751326	25.228562042659792	32.42516862394027	20.265610321648605
33	21.22433179581855	24.423528010496852	32.61814241831032	21.733997775374277
34	20.563763959142193	25.67752148262275	32.39577590065644	21.362938657578614
35	21.253724519102388	26.923926637299555	31.2704956612114	20.55185318238665
36	22.034456724497968	26.707995781279713	30.25865596489779	20.998891529324524
37	21.150081550398912	27.072523182790082	31.082228544752823	20.695166722058183
38	21.034431750288643	27.685255803141757	29.37399262684497	21.906319819724633
39	21.285038335411315	26.270370790164773	29.37005438614354	23.074536488280373
40	22.50887064704334	25.162188278643015	30.72039067347758	21.60855040083606
41	21.09888442128033	25.588670930212455	30.44298484065494	22.869459807852273
42	22.362195194577907	24.87344799697236	30.525207622128686	22.23914918632105
43	21.326341835450698	24.3116243417855	31.82866923965059	22.533364583113208
44	21.235378080712803	25.01167064012741	30.96369710510492	22.78925417405487
45	21.548516243802073	25.00235333895573	30.430978009248143	23.01815240799405
46	21.832645902212523	25.03981465294494	30.35740014638729	22.77013929845525
47	21.081690638705798	25.457076057993955	31.156190626218695	22.305042677081552
48	20.8513515849978	24.89092994350065	31.399112839241017	22.85860563226053
49	21.75080733934379	24.04017389734063	31.608896197580577	22.600122565735
50	20.67317020692093	24.30586106271024	31.888703396684576	23.132265333684256
51	20.64137611735573	24.005017894981528	30.757563823513024	24.596042164149715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	706.0
1	1283.5
2	1861.0
3	17979.5
4	34098.0
5	21877.5
6	9657.0
7	9382.0
8	9107.0
9	9054.0
10	9001.0
11	8890.0
12	8779.0
13	8366.5
14	7954.0
15	7586.5
16	7219.0
17	6798.0
18	6377.0
19	6289.5
20	6202.0
21	6089.5
22	5977.0
23	6042.0
24	6107.0
25	6909.5
26	8934.0
27	10156.0
28	11652.5
29	13149.0
30	14379.0
31	15609.0
32	18632.0
33	21655.0
34	24756.0
35	27857.0
36	30581.5
37	33306.0
38	35908.5
39	38511.0
40	42292.5
41	46074.0
42	49500.5
43	52927.0
44	57580.5
45	62234.0
46	71133.0
47	80032.0
48	83864.5
49	87697.0
50	85233.5
51	82770.0
52	74643.5
53	66517.0
54	60205.5
55	53894.0
56	50679.5
57	47465.0
58	44732.0
59	41999.0
60	39543.0
61	37087.0
62	33154.0
63	29221.0
64	25432.0
65	21643.0
66	19281.0
67	16919.0
68	14127.5
69	11336.0
70	9800.0
71	8264.0
72	7183.5
73	6103.0
74	5001.0
75	2945.0
76	1991.0
77	1465.5
78	940.0
79	729.5
80	519.0
81	435.0
82	351.0
83	239.0
84	127.0
85	77.0
86	27.0
87	20.0
88	13.0
89	12.0
90	11.0
91	9.5
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1041074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.297251790255686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.20681570545298	26.192966585084736
2	9.41628000883857	6.64737612799052
3	3.9159096630498302	4.146625480936954
4	2.1138184406013174	2.9844792694716133
5	1.3866649285879125	2.447273056654223
6	0.9853436998227738	2.086795480355194
7	0.7955427058370197	1.9656329838481417
8	0.6625654898089043	1.8709392737055186
9	0.5599914004381568	1.7789541715479165
>10	5.800020776286571	41.85941899519246
>50	0.12760145689218003	2.760026702953426
>100	0.025083394846380534	1.5890554790904072
>500	0.0016358735765529173	0.39610903288629495
>1k	0.0024538103648293758	1.2817726455326928
>5k	0.0	0.0
>10k+	2.7264559609215287E-4	1.9925747147499364
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20705	1.98881155422189	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2452	0.2355260048757341	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	1914	0.1838486025008789	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	1458	0.14004768152888267	Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1413	0.13572522222243566	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1349	0.1295777245421555	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1295	0.12439077337441912	Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1250	0.12006831406797211	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	1097	0.10537195242605232	Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	1091	0.10479562451852606	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.60546512543777E-5	0.0	0.0	0.147251780372961	0.0
2	9.60546512543777E-5	0.0	0.0	0.49410512605251883	0.0
3	9.60546512543777E-5	0.0	0.0	0.7974457147138436	0.0
4	9.60546512543777E-5	0.0	0.0	1.1100075498955886	0.0
5	9.60546512543777E-5	0.0	0.0	1.9208048611337907	0.0
6	9.60546512543777E-5	0.0	0.0	2.7947100782461187	0.0
7	2.8816395376313306E-4	0.0	0.0	3.5708316603814905	0.0
8	2.8816395376313306E-4	0.0	0.0	4.709079277745866	0.0
9	2.8816395376313306E-4	0.0	0.0	5.278491250381817	0.0
10	2.8816395376313306E-4	0.0	0.0	6.144231822137523	0.0
11	2.8816395376313306E-4	0.0	0.0	7.296887637190055	0.0
12	2.8816395376313306E-4	0.0	0.0	8.1459146996275	0.0
13	2.8816395376313306E-4	0.0	0.0	8.529364867434976	0.0
14	2.8816395376313306E-4	0.0	0.0	8.715710890868468	0.0
15	2.8816395376313306E-4	0.0	0.0	8.954406699235598	0.0
16	3.842186050175108E-4	0.0	0.0	9.382138061271341	0.0
17	3.842186050175108E-4	0.0	0.0	9.931378557143873	0.0
18	3.842186050175108E-4	0.0	0.0	10.511740760022823	0.0
19	4.8027325627188845E-4	0.0	0.0	10.900954206905562	0.0
20	5.763279075262661E-4	0.0	0.0	11.2638486793446	0.0
21	5.763279075262661E-4	0.0	0.0	11.72750448094948	0.0
22	5.763279075262661E-4	0.0	0.0	12.24091659190413	0.0
23	5.763279075262661E-4	0.0	0.0	12.735117772607904	0.0
24	5.763279075262661E-4	0.0	0.0	13.14383031369528	0.0
25	5.763279075262661E-4	0.0	0.0	13.506724786134319	0.0
26	5.763279075262661E-4	0.0	0.0	13.841091027150808	0.0
27	5.763279075262661E-4	0.0	0.0	14.179395508868726	0.0
28	6.723825587806438E-4	0.0	0.0	14.527977838270862	0.0
29	6.723825587806438E-4	0.0	0.0	14.89346578629377	0.0
30	6.723825587806438E-4	0.0	0.0	15.325423552984706	0.0
31	7.684372100350216E-4	0.0	0.0	15.70531969869577	0.0
32	7.684372100350216E-4	0.0	0.0	16.093188380460948	0.0
33	7.684372100350216E-4	0.0	0.0	16.479135969201035	0.0
34	7.684372100350216E-4	0.0	0.0	16.85730313118952	0.0
35	7.684372100350216E-4	0.0	0.0	17.24565208621097	0.0
36	7.684372100350216E-4	0.0	0.0	17.612004526095166	0.0
37	7.684372100350216E-4	0.0	0.0	17.985176846218426	0.0
38	7.684372100350216E-4	0.0	0.0	18.36776252216461	0.0
39	7.684372100350216E-4	0.0	0.0	18.784927872562374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9005	0.0	44.000557	1
CGTTATT	1215	0.0	42.59259	1
TCGATAG	45	1.9279469E-8	40.0	1
TATTACG	45	1.9279469E-8	40.0	1
TGCGTAG	45	1.9279469E-8	40.0	1
GCGTAAG	130	0.0	39.80769	1
CTGACGG	85	0.0	39.705883	2
ACGTGCG	40	3.457808E-7	39.375	1
TAACGGG	265	0.0	39.056602	3
GGTACGA	35	6.247723E-6	38.571426	8
CACGTAG	35	6.247723E-6	38.571426	1
TGTAACG	35	6.247723E-6	38.571426	1
TACGGGA	535	0.0	38.271027	4
ATTGTCG	30	1.1398478E-4	37.500004	1
TAGCCGT	30	1.1398478E-4	37.500004	44
GTTTTTT	10920	0.0	37.190933	2
ATTACGG	85	0.0	37.058823	2
ACGGGAT	615	0.0	36.951218	5
TCACGAC	55	2.748493E-9	36.81818	25
CGGTCTA	55	2.748493E-9	36.81818	31
>>END_MODULE