##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936011.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	712204
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.022329276443266	33.0	31.0	34.0	30.0	34.0
2	32.15624736732734	33.0	31.0	34.0	30.0	34.0
3	32.031704399301326	33.0	31.0	34.0	30.0	34.0
4	35.708806746381654	37.0	35.0	37.0	33.0	37.0
5	31.985345490898673	37.0	35.0	37.0	0.0	37.0
6	33.823390489241845	37.0	35.0	37.0	17.0	37.0
7	35.52090552706809	37.0	35.0	37.0	32.0	37.0
8	35.76876569072906	37.0	35.0	37.0	35.0	37.0
9	37.74800197696166	39.0	38.0	39.0	35.0	39.0
10	37.3194604354932	39.0	37.0	39.0	34.0	39.0
11	37.31352955052204	39.0	37.0	39.0	34.0	39.0
12	37.3085674890902	39.0	37.0	39.0	34.0	39.0
13	37.28336544023903	39.0	37.0	39.0	34.0	39.0
14	38.53339071389658	40.0	38.0	41.0	34.0	41.0
15	38.66950480480312	40.0	38.0	41.0	35.0	41.0
16	38.60067480665652	40.0	38.0	41.0	35.0	41.0
17	38.59825274780821	40.0	38.0	41.0	34.0	41.0
18	38.43267799675374	40.0	38.0	41.0	34.0	41.0
19	38.209393656873594	40.0	37.0	41.0	34.0	41.0
20	38.17727364631482	40.0	36.0	41.0	34.0	41.0
21	38.11607067637924	40.0	36.0	41.0	34.0	41.0
22	38.16125576379801	40.0	36.0	41.0	34.0	41.0
23	38.17722169490764	40.0	36.0	41.0	34.0	41.0
24	38.14244654621429	40.0	36.0	41.0	34.0	41.0
25	37.88874816765983	40.0	35.0	41.0	34.0	41.0
26	37.93043425760035	40.0	36.0	41.0	34.0	41.0
27	37.916602546461405	40.0	36.0	41.0	34.0	41.0
28	37.854301576514594	40.0	36.0	41.0	34.0	41.0
29	37.80534650184498	40.0	36.0	41.0	34.0	41.0
30	37.59973687314309	40.0	35.0	41.0	33.0	41.0
31	37.51872356796648	40.0	35.0	41.0	33.0	41.0
32	37.30372056320942	40.0	35.0	41.0	33.0	41.0
33	37.04270405670285	40.0	35.0	41.0	32.0	41.0
34	36.7972448905089	40.0	35.0	41.0	31.0	41.0
35	36.68884055691908	40.0	35.0	41.0	31.0	41.0
36	36.56281065537402	40.0	35.0	41.0	30.0	41.0
37	36.49242773138033	40.0	35.0	41.0	30.0	41.0
38	36.378218881107095	40.0	35.0	41.0	30.0	41.0
39	36.28132810262228	40.0	35.0	41.0	29.0	41.0
40	36.22619923505063	40.0	35.0	41.0	29.0	41.0
41	36.1245640294073	39.0	35.0	41.0	29.0	41.0
42	36.09325558407423	39.0	35.0	41.0	29.0	41.0
43	36.02543653222953	39.0	35.0	41.0	28.0	41.0
44	35.95404827830228	39.0	35.0	41.0	28.0	41.0
45	35.936703528764234	39.0	35.0	41.0	28.0	41.0
46	35.79278408995175	39.0	35.0	41.0	27.0	41.0
47	35.7091423243902	39.0	35.0	41.0	26.0	41.0
48	35.67232702989593	39.0	35.0	41.0	26.0	41.0
49	35.620038640614204	39.0	35.0	41.0	26.0	41.0
50	35.50395953968245	39.0	35.0	41.0	26.0	41.0
51	34.4784850969666	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	25.0
10	34.0
11	21.0
12	18.0
13	17.0
14	16.0
15	40.0
16	45.0
17	90.0
18	198.0
19	361.0
20	663.0
21	1253.0
22	1732.0
23	2858.0
24	4676.0
25	7554.0
26	10369.0
27	11124.0
28	10584.0
29	10248.0
30	10868.0
31	12843.0
32	15957.0
33	22802.0
34	37927.0
35	53046.0
36	51641.0
37	78194.0
38	133253.0
39	233555.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.511566910604266	19.38166592717817	23.937383109333844	16.169384052883725
2	36.751548713570834	22.557722225654448	24.2875636755761	16.403165385198623
3	30.23431488730757	22.6117797709645	31.73613178246682	15.41777355926111
4	25.725353971614872	24.361840146924195	32.30787807987599	17.60492780158494
5	21.391062111417515	35.93773694053951	27.09293966335488	15.578261284688095
6	23.013209698344856	33.867543568977425	29.34791155343132	13.771335179246396
7	75.10586854328254	5.6242312595829285	15.05004184194416	4.219858355190366
8	75.79752430483401	5.663686247198836	14.022106025801595	4.516683422165559
9	70.38025621872384	7.159184727971199	16.071238016074048	6.389321037230906
10	36.52956175477812	23.930222239695368	24.80511201846662	14.735103987059887
11	27.51753711015383	24.03440587247474	29.856052479345806	18.592004538025623
12	24.43794193798406	21.147732952917984	34.29466838153113	20.11965672756682
13	22.513914552572015	22.513914552572015	36.45388119134405	18.518289703511915
14	19.858495599575402	25.415611257448706	35.125048441176965	19.600844701798923
15	19.33757743567854	24.644062656205246	37.610010614936165	18.40834929318004
16	21.146890497666398	23.507871340233976	35.198061229647685	20.147176932451938
17	21.009710700866606	24.361278510089807	33.251568370860035	21.377442418183552
18	21.803163138651286	24.26523861140909	34.66633155668881	19.26526669325081
19	21.571909172091143	26.84610027464041	32.19288293803461	19.38910761523384
20	22.456346777047028	25.493117140594553	33.54825302862663	18.502283053731798
21	21.887970300644195	26.389629937489822	33.64485456414174	18.07754519772425
22	20.482474122582854	24.08411073231827	33.32486197774795	22.108553167350927
23	19.741534728813654	25.261722764825805	33.81180111316421	21.184941393196333
24	20.51771683394084	24.564871862556235	33.87947835170822	21.037932951794712
25	19.244486130378373	26.803696693643957	32.549943555498146	21.401873620479524
26	18.697311444473776	26.97443429129856	33.38664202953087	20.941612234696798
27	20.7477071176236	26.110636840006514	32.148794446534986	20.992861595834903
28	18.44836591763034	25.60867391927032	34.27711723045645	21.665842932642896
29	19.78815058606804	25.12075191939388	33.696805971322824	21.39429152321526
30	21.402435257313915	25.479076219734797	32.73921516868762	20.379273354263667
31	21.114596379688965	25.962645534144713	30.680114124604746	22.242643961561576
32	21.89807976366322	26.73686191035153	30.51021898220173	20.854839343783524
33	21.769464928587876	25.683652436661408	30.89550185059337	21.651380784157347
34	19.652515290562818	25.36941662782012	31.64739316263318	23.33067491898389
35	20.879270546079496	26.107267019000176	30.66789852345676	22.34556391146357
36	20.842202515009745	27.832615374246707	30.145576267473928	21.179605843269623
37	20.865370034428338	27.112175724932747	30.369248136769798	21.653206103869117
38	20.50788818933901	26.712851935681346	30.289776524703598	22.489483350276043
39	21.4204076360144	25.742624304272372	30.689521541580785	22.147446518132448
40	20.65307131102886	24.9714969306547	31.944639457234164	22.430792301082274
41	19.15925774075967	26.844836591763034	30.351416167277918	23.64448950019938
42	20.351472330961354	26.897770863404308	30.721254022723826	22.029502782910516
43	21.14450354112024	25.97710768263026	30.416285221649975	22.462103554599526
44	20.76567949632409	25.672419699973602	29.835131507264773	23.72676929643754
45	21.021785892805994	24.747123015315836	30.115388287625457	24.115702804252713
46	21.173849065717125	25.65669386861068	30.61608752548427	22.553369540187923
47	19.472370275932178	25.53973299784893	32.28120033024246	22.706696395976433
48	19.963240869189164	25.066273146458034	31.654834850688847	23.315651133663952
49	20.84360660709572	24.089586691453572	31.987323856647816	23.079482844802893
50	20.06447590858799	23.67594116292523	31.969772705573124	24.289810222913662
51	19.17526439053979	23.655441418469987	30.632094175264392	26.53720001572583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	343.0
1	701.0
2	1059.0
3	9180.0
4	17301.0
5	11219.0
6	5137.0
7	4938.5
8	4740.0
9	4771.0
10	4802.0
11	4875.0
12	4948.0
13	4686.0
14	4424.0
15	4265.0
16	4106.0
17	3922.5
18	3739.0
19	3720.5
20	3702.0
21	3845.5
22	3989.0
23	4364.5
24	4740.0
25	4811.5
26	5705.5
27	6528.0
28	7665.5
29	8803.0
30	10253.5
31	11704.0
32	13186.0
33	14668.0
34	16903.5
35	19139.0
36	21057.0
37	22975.0
38	24549.5
39	26124.0
40	29505.5
41	32887.0
42	36378.0
43	39869.0
44	44010.0
45	48151.0
46	56726.5
47	65302.0
48	67332.0
49	69362.0
50	67778.0
51	66194.0
52	59065.0
53	51936.0
54	46441.5
55	40947.0
56	36193.0
57	31439.0
58	28704.0
59	25969.0
60	23329.5
61	20690.0
62	18131.0
63	15572.0
64	12622.0
65	9672.0
66	7834.5
67	5997.0
68	5113.5
69	4230.0
70	3370.0
71	2510.0
72	2034.0
73	1558.0
74	1284.5
75	767.0
76	523.0
77	360.5
78	198.0
79	156.5
80	115.0
81	95.0
82	75.0
83	95.0
84	115.0
85	65.5
86	16.0
87	10.5
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	712204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.48497892386792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20449123832333	28.942432602870827
2	9.953990323970904	7.661582156528109
3	3.8285236745177955	4.420219587700402
4	2.001599381408956	3.081260400302029
5	1.2954176386643792	2.4927060260802687
6	0.9085465182332991	2.097923616333726
7	0.689037559010853	1.856231715540025
8	0.5657433539512492	1.741809684250574
9	0.48009777155622774	1.6628897357763641
>10	4.934016302944548	38.39214998528501
>50	0.1070507295991196	2.6154601103879327
>100	0.027410912689818774	1.9073884262012428
>500	0.0011112532171548145	0.31647251914549757
>1k	0.002592924173361234	1.4279753262524546
>5k	3.7041773905160485E-4	1.38349810734552
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9705	1.3626713694390933	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1798	0.2524557570583709	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	1682	0.23616828886105665	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	1619	0.22732250871941184	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	1401	0.19671330124514885	No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	1264	0.17747723966728635	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.1596452701753992	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC	1116	0.15669667679485091	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	944	0.13254629291607462	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	735	0.10320076831918945	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18393606326277304	0.0
2	0.0	0.0	0.0	0.7577884988009054	0.0
3	0.0	0.0	0.0	1.0808700877838373	0.0
4	0.0	0.0	0.0	1.4655913193410877	0.0
5	0.0	0.0	0.0	2.593077264379307	0.0
6	0.0	0.0	0.0	3.3858276561209992	0.0
7	0.0	0.0	0.0	4.135612830031845	0.0
8	0.0	0.0	0.0	5.326984964981944	0.0
9	0.0	0.0	0.0	5.798759905869667	0.0
10	0.0	0.0	0.0	7.069884471303166	0.0
11	0.0	0.0	0.0	8.590235381997292	0.0
12	0.0	0.0	0.0	9.912890126986088	0.0
13	1.4040920859753665E-4	0.0	0.0	10.43717811188929	0.0
14	1.4040920859753665E-4	0.0	0.0	10.667027986363458	0.0
15	1.4040920859753665E-4	0.0	0.0	11.03574256814059	0.0
16	1.4040920859753665E-4	0.0	0.0	11.763764314718816	0.0
17	1.4040920859753665E-4	0.0	0.0	12.623349489752936	0.0
18	1.4040920859753665E-4	0.0	0.0	13.571532875412101	0.0
19	1.4040920859753665E-4	0.0	0.0	14.19747712733992	0.0
20	1.4040920859753665E-4	0.0	0.0	14.797445675677194	0.0
21	2.808184171950733E-4	0.0	0.0	15.519008598659935	0.0
22	2.808184171950733E-4	0.0	0.0	16.28704696968846	0.0
23	2.808184171950733E-4	0.0	0.0	17.093136236246917	0.0
24	2.808184171950733E-4	0.0	0.0	17.720203761843518	0.0
25	2.808184171950733E-4	0.0	0.0	18.28422755277982	0.0
26	2.808184171950733E-4	0.0	0.0	18.80065262200156	0.0
27	2.808184171950733E-4	0.0	0.0	19.31539278072013	0.0
28	2.808184171950733E-4	0.0	0.0	19.87857411640485	0.0
29	4.2122762579261E-4	0.0	0.0	20.421676935260123	0.0
30	4.2122762579261E-4	0.0	0.0	21.009289473240813	0.0
31	4.2122762579261E-4	0.0	0.0	21.563203801158096	0.0
32	4.2122762579261E-4	0.0	0.0	22.114871581737816	0.0
33	4.2122762579261E-4	0.0	0.0	22.644073888941932	0.0
34	4.2122762579261E-4	0.0	0.0	23.15993732132928	0.0
35	4.2122762579261E-4	0.0	0.0	23.709218145362847	0.0
36	4.2122762579261E-4	0.0	0.0	24.22817058033934	0.0
37	4.2122762579261E-4	0.0	0.0	24.76986930710864	0.0
38	4.2122762579261E-4	0.0	0.0	25.307496166828606	0.0
39	4.2122762579261E-4	0.0	0.0	25.848071619929122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTGA	25	3.889305E-5	45.000004	30
TGGAACG	25	3.889305E-5	45.000004	1
AGGGCCG	50	2.1827873E-11	45.000004	6
CCGTTAG	25	3.889305E-5	45.000004	1
TAGTACG	25	3.889305E-5	45.000004	1
CTGTCGT	20	7.0314767E-4	45.0	28
GGTACGA	20	7.0314767E-4	45.0	8
TTGCGAG	35	1.211065E-7	45.0	1
CCGACCG	20	7.0314767E-4	45.0	15
GTATACG	40	6.8084773E-9	45.0	1
TATCACG	30	2.1643355E-6	44.999996	1
CGACGCG	30	2.1643355E-6	44.999996	13
CGTTTTT	4740	0.0	43.860756	1
CGTTATT	595	0.0	43.487396	1
TATACGG	70	0.0	41.785713	2
TAATACG	60	3.6379788E-12	41.249996	4
CGTAAGG	110	0.0	40.90909	2
TTGCGCG	50	1.0804797E-9	40.500004	1
CAACGAC	40	3.4557524E-7	39.375	10
CGCAATC	40	3.4557524E-7	39.375	18
>>END_MODULE