##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936010.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627538
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07179485545098	33.0	31.0	34.0	30.0	34.0
2	32.21281420408007	33.0	31.0	34.0	30.0	34.0
3	32.06729632309119	33.0	31.0	34.0	30.0	34.0
4	35.77736487670866	37.0	35.0	37.0	35.0	37.0
5	31.83119747330042	37.0	35.0	37.0	0.0	37.0
6	33.76694160353636	37.0	35.0	37.0	17.0	37.0
7	35.540558500043026	37.0	35.0	37.0	32.0	37.0
8	35.83833170262199	37.0	35.0	37.0	35.0	37.0
9	37.84563803307529	39.0	38.0	39.0	35.0	39.0
10	37.438113707855145	39.0	37.0	39.0	34.0	39.0
11	37.384277924205385	39.0	37.0	39.0	34.0	39.0
12	37.40917044067451	39.0	37.0	39.0	35.0	39.0
13	37.42454958902887	39.0	37.0	39.0	35.0	39.0
14	38.704559723873295	40.0	38.0	41.0	35.0	41.0
15	38.839765878719696	40.0	38.0	41.0	35.0	41.0
16	38.76018026000019	40.0	38.0	41.0	35.0	41.0
17	38.75384757576434	40.0	38.0	41.0	35.0	41.0
18	38.566180852792975	40.0	38.0	41.0	35.0	41.0
19	38.29916913398073	40.0	37.0	41.0	34.0	41.0
20	38.27327110071422	40.0	37.0	41.0	34.0	41.0
21	38.16436295491269	40.0	36.0	41.0	34.0	41.0
22	38.222947454974836	40.0	36.0	41.0	34.0	41.0
23	38.22557837134962	40.0	36.0	41.0	34.0	41.0
24	38.1687881849386	40.0	36.0	41.0	34.0	41.0
25	37.90066577641513	40.0	35.0	41.0	34.0	41.0
26	37.95819057969398	40.0	36.0	41.0	34.0	41.0
27	37.932810443351634	40.0	35.0	41.0	34.0	41.0
28	37.86261867807208	40.0	35.0	41.0	34.0	41.0
29	37.809310352520484	40.0	36.0	41.0	34.0	41.0
30	37.5867055062801	40.0	35.0	41.0	33.0	41.0
31	37.478124033923045	40.0	35.0	41.0	33.0	41.0
32	37.229920737867666	40.0	35.0	41.0	33.0	41.0
33	36.90281544703269	40.0	35.0	41.0	31.0	41.0
34	36.61230714315308	40.0	35.0	41.0	30.0	41.0
35	36.4684146617416	40.0	35.0	41.0	30.0	41.0
36	36.309891990604555	40.0	35.0	41.0	29.0	41.0
37	36.23264089186631	40.0	35.0	41.0	29.0	41.0
38	36.085688834779724	40.0	35.0	41.0	27.0	41.0
39	35.99661215735143	40.0	35.0	41.0	27.0	41.0
40	35.925912056321685	40.0	35.0	41.0	26.0	41.0
41	35.80189566209536	39.0	35.0	41.0	25.0	41.0
42	35.751543332834025	39.0	35.0	41.0	25.0	41.0
43	35.65230472098901	39.0	35.0	41.0	24.0	41.0
44	35.559298401053006	39.0	35.0	41.0	24.0	41.0
45	35.51481025850227	39.0	35.0	41.0	23.0	41.0
46	35.38994610684931	39.0	35.0	41.0	23.0	41.0
47	35.323685577606454	39.0	35.0	41.0	23.0	41.0
48	35.263034270434616	39.0	35.0	41.0	23.0	41.0
49	35.20855629459889	39.0	35.0	41.0	23.0	41.0
50	35.07707102996153	39.0	35.0	41.0	23.0	41.0
51	34.0978761445522	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	17.0
10	17.0
11	16.0
12	15.0
13	14.0
14	19.0
15	25.0
16	44.0
17	96.0
18	196.0
19	349.0
20	620.0
21	1003.0
22	1565.0
23	2657.0
24	4512.0
25	7573.0
26	10827.0
27	11651.0
28	10811.0
29	9955.0
30	9982.0
31	11375.0
32	13753.0
33	18602.0
34	30786.0
35	42673.0
36	45472.0
37	69544.0
38	118401.0
39	204779.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.1827076607313	19.766930448833378	24.253989399845107	17.796372490590212
2	37.53940000446188	21.723624704798752	24.580981550121265	16.1559937406181
3	29.24014163285729	22.39593458882171	32.94509655192196	15.41882722639904
4	26.49002928906297	24.201881001628585	31.701187816514697	17.606901892793744
5	20.19877680714156	36.82167454401168	27.94954249782483	15.03000615102193
6	23.50869588773907	31.879822417128523	30.390510216114404	14.220971479018004
7	76.18980842594392	5.119689962998257	14.706041705840923	3.984459905216895
8	78.18235708435186	4.243249014402315	14.012856591951403	3.561537309294417
9	72.51895502742464	7.1240307359873025	15.998075016971084	4.358939219616979
10	33.59015709008857	32.45397091490874	22.14320088982659	11.8126711051761
11	24.250164930251238	24.986215974172083	33.95969646459657	16.803922630980118
12	22.263671682033596	23.31476340874975	36.132473252615775	18.289091656600874
13	21.37751020655323	24.33239102651951	37.15233181098196	17.137766955945295
14	18.914870493898377	26.694479059435444	35.65011202508852	18.740538421577657
15	18.094521766012576	26.129094971141193	37.74257495163639	18.03380831120984
16	20.779618126711053	25.401170925107326	36.49261080603884	17.326600142142787
17	20.925426029977466	25.08517412491355	34.36461218284789	19.624787662261088
18	22.02209268602061	24.595482663998038	35.126797102326876	18.255627547654484
19	21.086372458719634	26.71806328859766	33.42968872004563	18.765875532637068
20	22.042649210087674	26.522059221911658	33.72544770197183	17.70984386602883
21	21.261820001338563	26.585322323110315	34.76426925540764	17.38858842014348
22	21.34006227511322	23.986595234073473	34.318399841921924	20.354942648891384
23	18.799020935783968	26.336731799508556	34.583085008397894	20.281162256309578
24	19.115177088877484	25.4833970213756	35.92483642424841	19.476589465498503
25	19.43117388907126	27.486303618266945	33.53071845848379	19.551804034178012
26	20.11575394637456	27.55147895426253	33.2966290487588	19.036138050604105
27	19.89775917952379	26.580382383218225	33.97802204806721	19.543836389190776
28	18.538160238901867	26.714398171903536	35.07962864400243	19.667812945192164
29	19.551485328378522	24.852837597085752	34.64555134509782	20.9501257294379
30	20.10969853618426	25.96049960321128	33.66075042467548	20.26905143592898
31	20.604648642791354	27.018124798816963	31.49514451714478	20.882082041246903
32	20.42075539648595	26.768100099117504	31.810503905739573	21.000640598656975
33	19.816807906453473	26.97079698759278	31.069034863227408	22.143360242726338
34	18.97654006609958	27.5613588340467	31.481918226465964	21.980182873387747
35	19.548298270383626	26.281755049096628	31.110307264261287	23.059639416258456
36	19.465275409616627	28.958405706108636	30.23944366715641	21.336875217118326
37	18.826270281640316	28.704078478116067	30.592410340090957	21.87724090015266
38	18.77671152981971	29.286035267983774	30.643084562209776	21.29416863998674
39	19.950345636439547	27.87241569434839	30.249642252740074	21.92759641647199
40	20.959846256322326	26.690495236941825	31.59187172728982	20.757786779446025
41	19.27548610602067	27.538730722282956	30.7992504039596	22.386532767736774
42	20.37502111425922	27.439135159942506	30.539664530275456	21.646179195522823
43	20.266501789533066	27.165048172381596	30.725788717177288	21.842661320908057
44	20.143640703829888	27.639441754921613	29.7828976093878	22.4340199318607
45	20.341078946613592	26.505805226137703	29.65222823159713	23.50088759565158
46	20.361794823580404	27.30623484155541	29.538450261179406	22.793520073684782
47	19.15517466671341	27.075013784025824	31.185203127141303	22.584608422119455
48	19.52312051222396	26.83789666920569	31.121940025942653	22.5170427926277
49	20.438124862558123	26.32270874433102	30.782518349486406	22.45664804362445
50	18.723647014204715	26.159372022092686	31.185840538740283	23.93114042496231
51	19.172862838585072	25.915562085483266	29.72584927127919	25.185725804652463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	606.0
2	888.0
3	8874.0
4	16860.0
5	11239.5
6	5619.0
7	5535.0
8	5451.0
9	5291.0
10	5131.0
11	5090.0
12	5049.0
13	4959.0
14	4869.0
15	4650.0
16	4431.0
17	4180.5
18	3930.0
19	3879.0
20	3828.0
21	3986.0
22	4144.0
23	4224.5
24	4305.0
25	5002.0
26	6384.0
27	7069.0
28	8384.5
29	9700.0
30	10919.5
31	12139.0
32	14051.5
33	15964.0
34	18088.5
35	20213.0
36	22478.0
37	24743.0
38	26197.5
39	27652.0
40	29752.0
41	31852.0
42	34389.5
43	36927.0
44	40690.0
45	44453.0
46	47893.5
47	51334.0
48	53956.5
49	56579.0
50	54447.5
51	52316.0
52	46845.0
53	41374.0
54	36903.5
55	32433.0
56	28790.5
57	25148.0
58	22181.0
59	19214.0
60	16696.0
61	14178.0
62	12491.0
63	10804.0
64	9300.0
65	7796.0
66	6289.0
67	4782.0
68	4155.5
69	3529.0
70	3082.5
71	2636.0
72	2169.0
73	1702.0
74	1408.5
75	938.5
76	762.0
77	564.5
78	367.0
79	233.0
80	99.0
81	80.5
82	62.0
83	36.0
84	10.0
85	30.0
86	50.0
87	27.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	627538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.520885195490614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36615266495834	29.031709144326783
2	9.158608772812823	7.055954341758717
3	3.6434675049215235	4.210487804117479
4	2.0299843218466243	3.127871720419987
5	1.332677237970506	2.566795344325269
6	0.9663681613916271	2.2335214200926523
7	0.7991148690757675	2.154782849077401
8	0.6342508651397181	1.9545523808950143
9	0.5568739392311931	1.9306149373336863
>10	5.416346717897768	40.05579351731738
>50	0.06760238475790309	1.7200024077031486
>100	0.02645310705532394	1.8104181808972306
>500	8.397811763594901E-4	0.2565284762711523
>1k	8.397811763594901E-4	0.35729596726543217
>5k	4.1989058817974506E-4	1.5336715081986545
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9482	1.5109841953794034	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.1835745405059136	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1057	0.1684360150301655	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	796	0.12684490819679448	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	790	0.12588879079832616	Illumina PCR Primer Index 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1870579948943333E-4	0.0	0.0	0.13274096548734898	0.0
2	3.1870579948943333E-4	0.0	0.0	0.5260239220573096	0.0
3	3.1870579948943333E-4	0.0	0.0	0.7639378013761716	0.0
4	3.1870579948943333E-4	0.0	0.0	1.061768370999047	0.0
5	3.1870579948943333E-4	0.0	0.0	1.787302123536742	0.0
6	3.1870579948943333E-4	0.0	0.0	2.55315215970985	0.0
7	3.1870579948943333E-4	0.0	0.0	3.2369354525144294	0.0
8	3.1870579948943333E-4	0.0	0.0	4.354796044223617	0.0
9	3.1870579948943333E-4	0.0	0.0	4.880023201782203	0.0
10	4.7805869923415E-4	0.0	0.0	5.8133531355870085	0.0
11	4.7805869923415E-4	0.0	0.0	6.930257609897727	0.0
12	4.7805869923415E-4	0.0	0.0	7.797296737408731	0.0
13	4.7805869923415E-4	0.0	0.0	8.21990062753172	0.0
14	4.7805869923415E-4	0.0	0.0	8.422756868906744	0.0
15	4.7805869923415E-4	0.0	0.0	8.670231922210288	0.0
16	4.7805869923415E-4	0.0	0.0	9.142713269953374	0.0
17	4.7805869923415E-4	0.0	0.0	9.768492107250875	0.0
18	4.7805869923415E-4	0.0	0.0	10.415783586013914	0.0
19	4.7805869923415E-4	0.0	0.0	10.861174940800398	0.0
20	4.7805869923415E-4	0.0	0.0	11.287762653417005	0.0
21	4.7805869923415E-4	0.0	0.0	11.812511752276356	0.0
22	4.7805869923415E-4	0.0	0.0	12.364988255691289	0.0
23	6.374115989788667E-4	0.0	0.0	12.977700155209725	0.0
24	6.374115989788667E-4	0.0	0.0	13.443488681163531	0.0
25	6.374115989788667E-4	0.0	0.0	13.858443632098773	0.0
26	6.374115989788667E-4	0.0	0.0	14.246627295876904	0.0
27	6.374115989788667E-4	0.0	0.0	14.665247363506273	0.0
28	6.374115989788667E-4	0.0	0.0	15.085301607233347	0.0
29	6.374115989788667E-4	0.0	0.0	15.551408838986642	0.0
30	6.374115989788667E-4	0.0	0.0	16.08779069952736	0.0
31	7.967644987235833E-4	0.0	0.0	16.549754755887292	0.0
32	7.967644987235833E-4	0.0	0.0	17.018092928237014	0.0
33	0.0011154702982130165	0.0	0.0	17.466830693918137	0.0
34	0.0011154702982130165	0.0	0.0	17.89564934713117	0.0
35	0.0011154702982130165	0.0	0.0	18.35856952088957	0.0
36	0.0012748231979577333	0.0	0.0	18.782926292909753	0.0
37	0.0012748231979577333	0.0	0.0	19.22608670709981	0.0
38	0.00143417609770245	0.0	0.0	19.675621237279653	0.0
39	0.0015935289974471666	0.0	0.0	20.14746517342376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	35	1.2107557E-7	45.000004	1
TAGCGCG	35	1.2107557E-7	45.000004	1
GTAACGT	30	2.163919E-6	45.000004	36
ACGTTGC	20	7.030836E-4	45.0	44
CGATTCA	20	7.030836E-4	45.0	10
TCTAGCG	25	3.8887745E-5	45.0	1
TAGCACG	25	3.8887745E-5	45.0	1
CGCACGG	40	6.8066583E-9	45.0	2
GTAAGCG	25	3.8887745E-5	45.0	1
CCAGTCG	20	7.030836E-4	45.0	28
TCGACTA	20	7.030836E-4	45.0	24
GCGTATG	25	3.8887745E-5	45.0	1
ACCACGG	55	1.8189894E-12	44.999996	2
CGTTATT	730	0.0	44.383564	1
CGTTTTT	5030	0.0	43.479126	1
TACGAAG	65	0.0	41.538464	1
GACACGA	160	0.0	40.78125	25
CACGACC	155	0.0	40.64516	27
GTACGAG	50	1.0804797E-9	40.5	1
ATGAGCG	40	3.4548793E-7	39.375	1
>>END_MODULE