##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936009.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922092
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05346104293281	33.0	31.0	34.0	30.0	34.0
2	32.201285771918634	33.0	31.0	34.0	30.0	34.0
3	32.06835868872086	33.0	31.0	34.0	30.0	34.0
4	35.75874858473992	37.0	35.0	37.0	35.0	37.0
5	32.12979182120656	37.0	35.0	37.0	0.0	37.0
6	33.8947469449903	37.0	35.0	37.0	17.0	37.0
7	35.53557671034994	37.0	35.0	37.0	32.0	37.0
8	35.787574341822726	37.0	35.0	37.0	35.0	37.0
9	37.737226871071435	39.0	38.0	39.0	35.0	39.0
10	37.34516187104974	39.0	37.0	39.0	34.0	39.0
11	37.3062579438928	39.0	37.0	39.0	34.0	39.0
12	37.259951284687425	39.0	37.0	39.0	34.0	39.0
13	37.24233048329234	39.0	37.0	39.0	34.0	39.0
14	38.50266567761135	40.0	38.0	41.0	34.0	41.0
15	38.61602638348451	40.0	38.0	41.0	35.0	41.0
16	38.53677398784503	40.0	38.0	41.0	34.0	41.0
17	38.51214520893794	40.0	38.0	41.0	34.0	41.0
18	38.3384749027212	40.0	38.0	41.0	34.0	41.0
19	38.103958173370984	40.0	37.0	41.0	34.0	41.0
20	38.08838597450146	40.0	36.0	41.0	34.0	41.0
21	37.99286296812032	40.0	36.0	41.0	34.0	41.0
22	38.036640595515415	40.0	36.0	41.0	34.0	41.0
23	38.040623929065646	40.0	35.0	41.0	34.0	41.0
24	37.998268068695964	40.0	35.0	41.0	34.0	41.0
25	37.73403304659405	40.0	35.0	41.0	33.0	41.0
26	37.78061625087302	40.0	35.0	41.0	34.0	41.0
27	37.76118868833045	40.0	35.0	41.0	34.0	41.0
28	37.65624905107082	40.0	35.0	41.0	33.0	41.0
29	37.60482468126825	40.0	35.0	41.0	33.0	41.0
30	37.39775423710432	40.0	35.0	41.0	33.0	41.0
31	37.291463324700786	40.0	35.0	41.0	33.0	41.0
32	37.05788901758176	40.0	35.0	41.0	32.0	41.0
33	36.77341198058328	40.0	35.0	41.0	31.0	41.0
34	36.49817588700477	40.0	35.0	41.0	30.0	41.0
35	36.37571955943658	40.0	35.0	41.0	30.0	41.0
36	36.23101274059422	40.0	35.0	41.0	29.0	41.0
37	36.177817397830154	40.0	35.0	41.0	29.0	41.0
38	36.040909150063115	39.0	35.0	41.0	28.0	41.0
39	35.962849693956784	39.0	35.0	41.0	28.0	41.0
40	35.88621200487587	39.0	35.0	41.0	27.0	41.0
41	35.77215288713057	39.0	35.0	41.0	27.0	41.0
42	35.75434772235308	39.0	35.0	41.0	27.0	41.0
43	35.67016740195122	39.0	35.0	41.0	26.0	41.0
44	35.604629472981	39.0	35.0	41.0	26.0	41.0
45	35.560252122347876	39.0	35.0	41.0	26.0	41.0
46	35.440302052289795	39.0	35.0	41.0	25.0	41.0
47	35.35109294951046	39.0	35.0	41.0	25.0	41.0
48	35.292611800124064	38.0	35.0	41.0	24.0	41.0
49	35.2510693076179	38.0	35.0	41.0	24.0	41.0
50	35.15854166395544	38.0	35.0	40.0	24.0	41.0
51	34.242341328197185	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	25.0
9	28.0
10	49.0
11	17.0
12	22.0
13	25.0
14	42.0
15	36.0
16	77.0
17	176.0
18	305.0
19	581.0
20	1053.0
21	1872.0
22	2777.0
23	4093.0
24	6808.0
25	10682.0
26	14697.0
27	15426.0
28	14792.0
29	13862.0
30	14902.0
31	17663.0
32	21857.0
33	30921.0
34	52353.0
35	70015.0
36	67671.0
37	102483.0
38	169027.0
39	287535.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06877838653843	19.072391908833392	21.591663304746163	19.267166399882008
2	36.68354133860829	23.172308186167974	23.11971039766097	17.024440077562762
3	29.291871093123028	22.959531153073662	31.62764669902786	16.120951054775446
4	26.278722730486763	23.82853337844814	31.655518104484155	18.23722578658095
5	21.55359768873388	35.105390785301246	26.68302078317565	16.657990742789224
6	23.379445868741946	32.683940431106656	29.356289827913052	14.580323872238345
7	74.84502630973915	5.51344117506713	15.124412748402547	4.517119766791166
8	76.75427180801915	5.198071342122044	13.966176910763787	4.081479939095015
9	70.82460318493166	6.88293575912165	15.94721567912963	6.345245376817064
10	37.14531738698525	25.4918164348026	22.94727641059677	14.415589767615378
11	28.81556287225136	23.635819419320413	29.868603132876114	17.680014575552114
12	25.78484576376327	21.25948386928853	33.09301024192814	19.862660125020064
13	23.053990274289333	22.620410978514073	34.94575378595628	19.379844961240313
14	19.803663842653442	25.621521496770388	34.134988699609146	20.439825960967017
15	19.095166209011683	24.787982110244965	36.399621729718945	19.71722995102441
16	22.04769155355431	23.86063429679468	34.55826533577994	19.533408813871066
17	21.407842167592822	24.54310415880411	32.8314311370232	21.217622536579864
18	22.791977373190527	24.122647197893485	32.58048003886814	20.504895390047846
19	22.26675863145977	26.931694451312882	30.498692104475477	20.30285481275187
20	24.696234215240995	25.25507216199685	31.111754575465355	18.9369390472968
21	23.02460058215449	26.523492232879146	31.54208039978657	18.90982678517979
22	22.146488636708703	23.989797113520126	32.17054263565892	21.69317161411226
23	21.726031675798076	25.941988434993473	31.908204387414706	20.42377550179375
24	21.843373546240503	23.777020080425814	32.84140844948226	21.538197923851417
25	20.951922367833145	25.749274475865747	31.295358814521762	22.003444341779346
26	20.747279013373937	25.73712818243733	31.788801985051386	21.726790819137353
27	21.471610208092034	25.07786641680006	31.19038013560469	22.260143239503215
28	19.753018137018866	26.642786186194	32.11588431523102	21.488311361556114
29	21.09182164035693	25.31081497290943	31.184415437938945	22.4129479487947
30	20.91114552560916	25.052706237555473	31.764834745339943	22.271313491495427
31	21.5134715408007	26.29054367676978	29.76926380447938	22.42672097795014
32	22.431058939888864	27.19023698286071	29.16184068400984	21.216863393240587
33	21.677771849229796	27.096320106887383	28.675988946872984	22.549919097009845
34	20.273573569665498	27.623599380539037	30.11792749530416	21.984899554491307
35	20.647505888783332	27.37188913904469	29.323646664324166	22.65695830784781
36	21.098220134216543	29.7657934349284	27.214421120669087	21.92156531018597
37	21.64024847845985	28.764049574229034	28.165844622879277	21.429857324431836
38	21.263821831227254	29.48078933555437	27.94450011495599	21.310888718262387
39	21.957028149035022	26.99079918272797	28.64486406996265	22.40730859827436
40	22.732005049387695	25.42598786238249	30.16521128043623	21.676795807793585
41	19.893242756688053	26.623265357469755	29.76243151442589	23.7210603714163
42	21.7880645315218	26.037748944790756	28.61579972497321	23.558386798714228
43	21.226515358554245	25.993501733015794	28.81827409846306	23.9617088099669
44	21.474972128594544	26.563943727957728	28.228094376699943	23.732989766747785
45	21.281499026127545	25.56979130065113	28.782594361517074	24.366115311704256
46	21.87764344555641	27.08970471493083	27.64149347353626	23.391158365976498
47	20.610199416110323	26.679116617430797	30.03377103369295	22.676912932765926
48	21.042911119497838	25.385536367303914	29.930635988599835	23.640916524598413
49	21.102341198058326	25.435422929599216	29.890943636860527	23.571292235481923
50	19.639038187078945	25.39128416687272	30.450106930761788	24.51957071528654
51	19.366614177327207	25.05877938426968	29.45194188866187	26.12266454974124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	485.0
1	1061.5
2	1638.0
3	11495.5
4	21353.0
5	14224.5
6	7096.0
7	6896.5
8	6697.0
9	6679.0
10	6661.0
11	6551.0
12	6441.0
13	6273.0
14	6105.0
15	5904.5
16	5704.0
17	5295.0
18	4886.0
19	4773.5
20	4661.0
21	4858.0
22	5055.0
23	5305.5
24	5556.0
25	5687.5
26	7033.0
27	8247.0
28	9955.5
29	11664.0
30	12944.0
31	14224.0
32	16097.0
33	17970.0
34	20928.5
35	23887.0
36	25502.5
37	27118.0
38	29694.5
39	32271.0
40	36228.0
41	40185.0
42	43623.0
43	47061.0
44	52496.5
45	57932.0
46	67299.5
47	76667.0
48	77247.0
49	77827.0
50	75995.0
51	74163.0
52	68102.0
53	62041.0
54	56490.0
55	50939.0
56	47101.0
57	43263.0
58	41426.0
59	39589.0
60	38309.5
61	37030.0
62	33573.5
63	30117.0
64	26210.5
65	22304.0
66	18031.5
67	13759.0
68	11276.0
69	8793.0
70	7511.5
71	6230.0
72	5227.5
73	4225.0
74	3563.5
75	2228.0
76	1554.0
77	1292.0
78	1030.0
79	750.0
80	470.0
81	379.0
82	288.0
83	183.0
84	78.0
85	63.5
86	49.0
87	40.0
88	31.0
89	23.0
90	15.0
91	12.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	922092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.12554903811429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55643892026555	26.188358514009607
2	9.134038278537824	6.416762189375868
3	3.765208249664072	3.9676502103686353
4	2.1544645205022808	3.0270699666312106
5	1.390197995055973	2.4415733924013376
6	1.0308315454628623	2.172511440011455
7	0.7604633559099682	1.8698185029793184
8	0.6384441008292697	1.7940559657418642
9	0.5410360641356023	1.7103769921965106
>10	5.869317521926046	42.832275834958914
>50	0.12596805484346885	2.7665817472207075
>100	0.02861489022016731	1.7265013336529023
>500	0.0018661884926096356	0.5097674633169543
>1k	0.0027992827389144532	1.3235818298510291
>5k	0.0	0.0
>10k+	3.1103141543493924E-4	1.2531146172836405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11470	1.243910585928519	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	1878	0.20366731302299554	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	1709	0.1853394238318953	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	1353	0.14673156257726996	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1334	0.14467103065637701	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	1234	0.13382612580957215	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	1226	0.13295853342182776	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT	1206	0.13078955245246676	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	1091	0.11831791187864119	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC	1084	0.11755876853936484	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1689809693609748E-4	0.0	0.0	0.10042381888141313	0.0
2	2.1689809693609748E-4	0.0	0.0	0.44865371351231764	0.0
3	2.1689809693609748E-4	0.0	0.0	0.6966766873587451	0.0
4	2.1689809693609748E-4	0.0	0.0	1.001310064505494	0.0
5	2.1689809693609748E-4	0.0	0.0	1.9080525587468495	0.0
6	2.1689809693609748E-4	0.0	0.0	2.8892995492857545	0.0
7	2.1689809693609748E-4	0.0	0.0	3.666879226801664	0.0
8	2.1689809693609748E-4	0.0	0.0	4.65636834502414	0.0
9	2.1689809693609748E-4	0.0	0.0	5.189720765390004	0.0
10	2.1689809693609748E-4	0.0	0.0	6.1645692620692945	0.0
11	2.1689809693609748E-4	0.0	0.0	7.107750636595915	0.0
12	2.1689809693609748E-4	0.0	0.0	7.970896613353115	0.0
13	2.1689809693609748E-4	0.0	0.0	8.344286687228607	0.0
14	2.1689809693609748E-4	0.0	0.0	8.523769862443228	0.0
15	2.1689809693609748E-4	0.0	0.0	8.765068995284635	0.0
16	2.1689809693609748E-4	0.0	0.0	9.146701196843699	1.0844904846804874E-4
17	2.1689809693609748E-4	0.0	0.0	9.603163241845715	1.0844904846804874E-4
18	2.1689809693609748E-4	0.0	0.0	10.112439973451673	1.0844904846804874E-4
19	2.1689809693609748E-4	0.0	0.0	10.47162322197785	1.0844904846804874E-4
20	2.1689809693609748E-4	0.0	0.0	10.785474768244383	1.0844904846804874E-4
21	2.1689809693609748E-4	0.0	0.0	11.158431045926003	1.0844904846804874E-4
22	2.1689809693609748E-4	0.0	0.0	11.596131405543048	1.0844904846804874E-4
23	2.1689809693609748E-4	0.0	0.0	12.059859536792425	1.0844904846804874E-4
24	2.1689809693609748E-4	0.0	0.0	12.41860898912473	1.0844904846804874E-4
25	2.1689809693609748E-4	0.0	0.0	12.756210877005765	1.0844904846804874E-4
26	2.1689809693609748E-4	0.0	0.0	13.075593324744169	1.0844904846804874E-4
27	2.1689809693609748E-4	0.0	0.0	13.401482715390655	1.0844904846804874E-4
28	2.1689809693609748E-4	0.0	0.0	13.72693830984327	1.0844904846804874E-4
29	2.1689809693609748E-4	0.0	0.0	14.066926076790603	1.0844904846804874E-4
30	2.1689809693609748E-4	0.0	0.0	14.467103065637701	1.0844904846804874E-4
31	3.253471454041462E-4	0.0	0.0	14.83561293233213	1.0844904846804874E-4
32	4.3379619387219496E-4	0.0	0.0	15.210195945740772	1.0844904846804874E-4
33	4.3379619387219496E-4	0.0	0.0	15.568620050927674	1.0844904846804874E-4
34	5.422452423402437E-4	0.0	0.0	15.901992425918454	1.0844904846804874E-4
35	5.422452423402437E-4	0.0	0.0	16.25868134632987	1.0844904846804874E-4
36	5.422452423402437E-4	0.0	0.0	16.600512747101156	1.0844904846804874E-4
37	5.422452423402437E-4	0.0	0.0	16.95503268654321	1.0844904846804874E-4
38	5.422452423402437E-4	0.0	0.0	17.345991506270526	1.0844904846804874E-4
39	5.422452423402437E-4	0.0	0.0	17.71797174251593	1.0844904846804874E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACT	20	7.0325605E-4	45.0	17
TGTATCG	20	7.0325605E-4	45.0	40
CGTACTT	20	7.0325605E-4	45.0	10
GGACCGT	30	2.165034E-6	44.999996	8
CGTTTTT	6245	0.0	43.16253	1
CGTTATT	835	0.0	42.574852	1
ATTACGG	80	0.0	42.1875	2
TTACGGG	270	0.0	41.666664	3
AGTAACG	50	1.0804797E-9	40.500004	1
CGCGGAT	40	3.4572258E-7	39.375	23
TCGTGCG	40	3.4572258E-7	39.375	1
TAACGGG	190	0.0	39.078945	3
TACGGGT	70	0.0	38.571426	4
TAAGGGA	805	0.0	38.291927	4
GTAACGG	100	0.0	38.250004	2
ACTCTCG	30	1.1397469E-4	37.499996	44
CGACAGT	30	1.1397469E-4	37.499996	2
CGTTATG	30	1.1397469E-4	37.499996	1
CCGTAAG	30	1.1397469E-4	37.499996	1
GCTTACG	30	1.1397469E-4	37.499996	1
>>END_MODULE