##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936008.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808053
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09781660361387	33.0	31.0	34.0	30.0	34.0
2	32.22639604085376	34.0	31.0	34.0	30.0	34.0
3	32.11491325445237	33.0	31.0	34.0	30.0	34.0
4	35.78071735393594	37.0	35.0	37.0	35.0	37.0
5	32.04285114961519	37.0	35.0	37.0	0.0	37.0
6	33.86964345160528	37.0	35.0	37.0	17.0	37.0
7	35.550819067561164	37.0	35.0	37.0	32.0	37.0
8	35.80643843906278	37.0	35.0	37.0	35.0	37.0
9	37.79121419015832	39.0	38.0	39.0	35.0	39.0
10	37.33321824187275	39.0	37.0	39.0	34.0	39.0
11	37.3331563647434	39.0	37.0	39.0	34.0	39.0
12	37.336409864204455	39.0	37.0	39.0	35.0	39.0
13	37.337190753576806	39.0	37.0	39.0	34.0	39.0
14	38.59934063730968	40.0	38.0	41.0	34.0	41.0
15	38.73864585615053	40.0	38.0	41.0	35.0	41.0
16	38.650009343446534	40.0	38.0	41.0	35.0	41.0
17	38.645890801717215	40.0	38.0	41.0	35.0	41.0
18	38.48561171111301	40.0	38.0	41.0	35.0	41.0
19	38.25592628206318	40.0	37.0	41.0	34.0	41.0
20	38.25675296051126	40.0	37.0	41.0	34.0	41.0
21	38.165520083459874	40.0	36.0	41.0	34.0	41.0
22	38.20873383305303	40.0	36.0	41.0	34.0	41.0
23	38.21719862434766	40.0	36.0	41.0	34.0	41.0
24	38.166898705901716	40.0	36.0	41.0	34.0	41.0
25	37.89931972283996	40.0	36.0	41.0	34.0	41.0
26	37.929641991305026	40.0	36.0	41.0	34.0	41.0
27	37.92639344201432	40.0	36.0	41.0	34.0	41.0
28	37.8577543799726	40.0	36.0	41.0	34.0	41.0
29	37.81544651155308	40.0	36.0	41.0	34.0	41.0
30	37.60868779646879	40.0	35.0	41.0	33.0	41.0
31	37.509409655059756	40.0	35.0	41.0	33.0	41.0
32	37.306102446250435	40.0	35.0	41.0	33.0	41.0
33	37.06586944173216	40.0	35.0	41.0	32.0	41.0
34	36.82391501547547	40.0	35.0	41.0	31.0	41.0
35	36.695253900424845	40.0	35.0	41.0	31.0	41.0
36	36.55461832330305	40.0	35.0	41.0	30.0	41.0
37	36.48953100848583	40.0	35.0	41.0	30.0	41.0
38	36.36620122689972	40.0	35.0	41.0	30.0	41.0
39	36.26412500170162	40.0	35.0	41.0	29.0	41.0
40	36.20895164054833	40.0	35.0	41.0	29.0	41.0
41	36.09202490430702	39.0	35.0	41.0	29.0	41.0
42	36.07037533429119	39.0	35.0	41.0	29.0	41.0
43	35.99809665950129	39.0	35.0	41.0	28.0	41.0
44	35.91522090753948	39.0	35.0	41.0	28.0	41.0
45	35.881794882266384	39.0	35.0	41.0	27.0	41.0
46	35.77014255253059	39.0	35.0	41.0	27.0	41.0
47	35.69689611943771	39.0	35.0	41.0	27.0	41.0
48	35.63395594100882	39.0	35.0	41.0	26.0	41.0
49	35.57536696231559	39.0	35.0	41.0	27.0	41.0
50	35.46533333828351	39.0	35.0	41.0	26.0	41.0
51	34.51811205453107	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	20.0
9	30.0
10	34.0
11	22.0
12	21.0
13	18.0
14	31.0
15	42.0
16	63.0
17	122.0
18	249.0
19	498.0
20	793.0
21	1376.0
22	2150.0
23	3259.0
24	5555.0
25	8713.0
26	11797.0
27	12427.0
28	11897.0
29	11322.0
30	12070.0
31	14102.0
32	17755.0
33	25019.0
34	42411.0
35	58022.0
36	59499.0
37	90236.0
38	151035.0
39	267256.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61614770318284	18.966453933095973	23.272483364333777	17.144914999387417
2	37.16971535282958	22.543447026370796	23.85152954076032	16.435308080039306
3	30.87297491624931	22.13196411621515	30.535002035757557	16.46005893177799
4	26.84056615098267	24.788101770552178	30.412980336685834	17.958351741779314
5	21.390923615158908	36.22212899401401	25.99705712372827	16.389890267098817
6	23.961670830997473	33.572921578163815	28.469172195388175	13.996235395450546
7	76.56465603122568	5.886495069011563	13.346401783051359	4.2024471167114035
8	78.42332124254226	5.186912244617617	12.5830855154303	3.8066809974098232
9	73.28962332916281	7.016618959399941	14.450289770596731	5.243467940840514
10	36.65366009407799	25.522830804415058	22.88117239834516	14.942336703161798
11	27.952374411084424	25.362816547924457	28.572507001397184	18.11230203959394
12	24.638482871791826	22.361033249056682	33.47614574786555	19.524338131285944
13	23.299214284211555	23.59832832747357	34.38771961740134	18.71473777091354
14	20.202882731702005	26.50185074493876	33.53616656333186	19.759099960027374
15	19.755511086525264	25.45278589399458	35.804582125182385	18.987120894297774
16	21.96737095215289	24.233930200123012	34.59884438273232	19.199854464991777
17	22.192851211492314	24.607296798601084	32.14269361044387	21.057158379462734
18	22.71336162355687	24.48230499732072	33.018750007734646	19.78558337138777
19	22.918298675953185	26.950831195478514	30.56482681210267	19.566043316465628
20	23.564667169108958	25.792120071331954	31.64594401604845	18.997268743510638
21	23.10368255547594	26.378715257538797	31.488281090473023	19.029321096512234
22	21.96266829032254	24.621528538350827	31.589017057049475	21.82678611427716
23	20.82338658479085	26.439602352815967	31.853356153618634	20.88365490877455
24	21.040946571573894	24.768053580643844	33.45993393997671	20.73106590780555
25	21.41047678803247	26.429206995085718	31.16453994973102	20.99577626715079
26	20.28282798281796	27.27234475956404	31.305619804641527	21.139207452976475
27	21.03352131605229	25.905107709519054	32.12672931107242	20.934641663356242
28	19.957973053747715	26.469055866385	32.92791438185367	20.64505669801362
29	20.92820644190418	25.642996189606375	32.15915292685009	21.26964444163935
30	21.808099221214448	25.312696073153617	32.10767115523363	20.771533550398303
31	22.16587278309715	25.6199778974894	30.77558031465758	21.438569004755877
32	21.96699968937681	26.440468632626818	30.921610339915823	20.670921338080547
33	21.804262839194955	26.208181889059258	30.187005060311638	21.80055021143415
34	20.841702215077476	27.534456279476714	30.215839802587208	21.408001702858602
35	20.68725690022808	27.233114659558222	29.478635683550458	22.60099275666324
36	21.15999816843697	28.819396747490572	28.809867669571176	21.210737414501278
37	20.320201768943374	29.00044922795906	29.818217369405225	20.861131633692345
38	20.34198251847342	28.965303018490125	29.275678699293238	21.41703576374322
39	20.75160911474866	28.266586473907036	29.27543119077585	21.70637322056845
40	21.641030971978324	27.580740372228057	29.57033758924229	21.207891066551326
41	19.64957744108369	27.46998031069744	29.56848127536189	23.31196097285698
42	20.916697295845694	27.758203979194434	28.94216097211445	22.382937752845418
43	21.280411062145678	26.649242067042632	29.7452023567761	22.32514451403559
44	21.048619335612887	27.194874593621954	29.748419967502134	22.00808610326303
45	21.18499652869304	26.85789174719975	29.1766752923385	22.78043643176871
46	21.567397188055732	27.280265032120415	28.55010748057368	22.602230299250174
47	20.900856750732935	26.951697475289365	29.702754646044255	22.44469112793344
48	20.498160392944524	26.352108091919714	30.214602260000273	22.935129255135493
49	21.00679039617451	26.005224904802038	30.37709160166474	22.610893097358716
50	19.96081940169766	26.243575607045578	30.677195678996306	23.118409312260457
51	19.77407422532928	26.103485786204615	29.668227207868792	24.454212780597313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	507.0
1	829.5
2	1152.0
3	8939.0
4	16726.0
5	11193.5
6	5661.0
7	5443.5
8	5226.0
9	5203.5
10	5181.0
11	5118.5
12	5056.0
13	5019.0
14	4982.0
15	4768.0
16	4554.0
17	4338.5
18	4123.0
19	4221.5
20	4320.0
21	4195.0
22	4070.0
23	4189.5
24	4309.0
25	5119.0
26	6824.5
27	7720.0
28	9395.5
29	11071.0
30	12540.5
31	14010.0
32	16354.0
33	18698.0
34	20872.0
35	23046.0
36	25390.0
37	27734.0
38	29556.0
39	31378.0
40	35823.0
41	40268.0
42	44573.5
43	48879.0
44	56136.5
45	63394.0
46	66837.5
47	70281.0
48	68901.0
49	67521.0
50	62571.5
51	57622.0
52	53645.5
53	49669.0
54	46205.0
55	42741.0
56	39415.5
57	36090.0
58	33532.5
59	30975.0
60	29109.0
61	27243.0
62	24682.5
63	22122.0
64	18468.5
65	14815.0
66	12692.0
67	10569.0
68	8945.0
69	7321.0
70	5985.0
71	4649.0
72	4079.0
73	3509.0
74	3002.5
75	1830.0
76	1164.0
77	855.0
78	546.0
79	468.0
80	390.0
81	250.5
82	111.0
83	85.5
84	60.0
85	92.5
86	125.0
87	75.5
88	26.0
89	15.5
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	808053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.768644920507626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35863785669235	26.597077142686455
2	9.290180406152341	6.645943283902408
3	3.8316299539137693	4.111566338649679
4	2.1493516194232383	3.0751757953787138
5	1.4428018569874692	2.580353365661691
6	1.0643413790665162	2.284202931722018
7	0.8076192198142848	2.0221211573160183
8	0.6357223166108635	1.8191140648717203
9	0.5559655188248487	1.7897519907802633
>10	5.682649462686986	42.34150322979268
>50	0.15482859555551245	3.3621477510725803
>100	0.02381977827312195	1.6498752477772765
>500	0.0014011634278306405	0.32739398712347517
>1k	7.005817139153202E-4	0.2667515494014078
>5k	3.502908569576601E-4	1.1270221638636275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8995	1.1131695569473785	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.13254081106066062	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1058	0.13093200569764607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4750851738685457E-4	0.0	0.0	0.09120688865705591	0.0
2	2.4750851738685457E-4	0.0	0.0	0.38202939658661006	0.0
3	2.4750851738685457E-4	0.0	0.0	0.5750860401483566	0.0
4	2.4750851738685457E-4	0.0	0.0	0.8013088250399417	0.0
5	2.4750851738685457E-4	0.0	0.0	1.4198326099896912	0.0
6	2.4750851738685457E-4	0.0	0.0	2.1329046485812193	0.0
7	2.4750851738685457E-4	0.0	0.0	2.692397652134204	0.0
8	2.4750851738685457E-4	0.0	0.0	3.5449407402732245	0.0
9	2.4750851738685457E-4	0.0	0.0	3.956547404687564	0.0
10	2.4750851738685457E-4	0.0	0.0	4.752906059379768	0.0
11	2.4750851738685457E-4	0.0	0.0	5.618567098940292	0.0
12	2.4750851738685457E-4	0.0	0.0	6.338940638794733	0.0
13	2.4750851738685457E-4	0.0	0.0	6.67790355335603	0.0
14	2.4750851738685457E-4	0.0	0.0	6.842744225935675	0.0
15	2.4750851738685457E-4	0.0	0.0	7.04693875277983	0.0
16	2.4750851738685457E-4	0.0	0.0	7.462258044954972	0.0
17	3.712627760802819E-4	0.0	0.0	7.970145522632798	0.0
18	3.712627760802819E-4	0.0	0.0	8.535083713568293	0.0
19	3.712627760802819E-4	0.0	0.0	8.879986832546875	0.0
20	3.712627760802819E-4	0.0	0.0	9.226498756888471	0.0
21	4.950170347737091E-4	0.0	0.0	9.659143645280693	0.0
22	4.950170347737091E-4	0.0	0.0	10.1146830715312	0.0
23	4.950170347737091E-4	0.0	0.0	10.615269047946112	0.0
24	4.950170347737091E-4	0.0	0.0	11.015861583336736	0.0
25	4.950170347737091E-4	0.0	0.0	11.367571186543456	0.0
26	4.950170347737091E-4	0.0	0.0	11.696014989115813	0.0
27	4.950170347737091E-4	0.0	0.0	12.047105821029065	0.0
28	4.950170347737091E-4	0.0	0.0	12.404260611618298	0.0
29	4.950170347737091E-4	0.0	0.0	12.797056628711236	0.0
30	4.950170347737091E-4	0.0	0.0	13.242200697231494	0.0
31	6.187712934671364E-4	0.0	0.0	13.65801500644141	0.0
32	6.187712934671364E-4	0.0	0.0	14.06566153457756	0.0
33	6.187712934671364E-4	0.0	0.0	14.465140281639941	0.0
34	6.187712934671364E-4	0.0	0.0	14.828235276646458	0.0
35	6.187712934671364E-4	0.0	0.0	15.222516344843717	0.0
36	7.425255521605638E-4	0.0	0.0	15.59526417202832	0.0
37	7.425255521605638E-4	0.0	0.0	16.004519505527483	0.0
38	7.425255521605638E-4	0.0	0.0	16.406968354798508	0.0
39	7.425255521605638E-4	0.0	0.0	16.831692970634354	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCAACG	20	7.0320413E-4	45.0	1
TACGTCG	35	1.2113378E-7	45.0	1
CGTTTTT	4950	0.0	43.590908	1
CGTTATT	610	0.0	42.786884	1
GTACGAG	45	1.9270374E-8	40.0	1
CTTACGG	45	1.9270374E-8	40.0	2
AATGCGG	120	0.0	39.374996	2
TACGAAT	75	0.0	39.0	12
AAGGGCG	320	0.0	38.671875	5
GTTGCGT	35	6.245986E-6	38.571426	18
GCGTATG	65	9.094947E-12	38.07692	1
TTACGGG	270	0.0	37.500004	3
AACGATA	30	1.139622E-4	37.499996	10
CACGTAG	30	1.139622E-4	37.499996	1
ATACCGG	30	1.139622E-4	37.499996	2
TACGGGA	290	0.0	37.241383	4
GCTACGA	85	0.0	37.058823	10
CTTGCGG	140	0.0	36.964283	2
ACGGGAC	250	0.0	36.9	5
GATACGA	25	0.002106739	36.0	43
>>END_MODULE