FastQCFastQC Report
Sat 14 Jan 2017
SRR2936007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671374
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.4037987768367557No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC18450.27480956962885067No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG15990.23816829367833725No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC15240.22699717296171731No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC10790.16071519004310564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG302.1641463E-645.0000041
TCGCCGT207.031188E-445.039
CGTAATG207.031188E-445.043
GCGTAAG750.042.01
TACGAAT650.041.53846412
CGAATAT650.041.53846414
CGTAAGG1900.040.2631572
ATAACGG451.9263098E-840.02
CGTTTTT14950.039.732441
CGGAAAT403.4553523E-739.37523
TTACGCG356.244407E-638.571431
GCTACGA700.038.5714310
CGTTATT1950.038.0769231
ATCGATT301.1394159E-437.50000443
GCGTTAG1150.037.1739161
CGACAGG800.036.56252
TACGGGA2300.036.1956564
GTACGGG2550.036.176473
ACGGGGT2800.036.1607175
TTTCGCG250.002106486136.01
Produced by FastQC (version 0.11.5)