##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936007.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671374
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17605239404564	33.0	31.0	34.0	30.0	34.0
2	32.28752826293541	34.0	31.0	34.0	30.0	34.0
3	32.24186072144587	34.0	31.0	34.0	30.0	34.0
4	35.84787316756383	37.0	35.0	37.0	35.0	37.0
5	32.07647600294322	37.0	35.0	37.0	0.0	37.0
6	33.90167775338336	37.0	35.0	37.0	17.0	37.0
7	35.56204291497735	37.0	35.0	37.0	32.0	37.0
8	35.804305796769015	37.0	35.0	37.0	35.0	37.0
9	37.77267514083059	39.0	38.0	39.0	35.0	39.0
10	37.354178148096295	39.0	37.0	39.0	34.0	39.0
11	37.34110942634061	39.0	37.0	39.0	34.0	39.0
12	37.31711385904131	39.0	37.0	39.0	35.0	39.0
13	37.2671402228862	39.0	37.0	39.0	34.0	39.0
14	38.54318904217322	40.0	38.0	41.0	34.0	41.0
15	38.67203823800147	40.0	38.0	41.0	35.0	41.0
16	38.61692439683396	40.0	38.0	41.0	35.0	41.0
17	38.60110757938198	40.0	38.0	41.0	35.0	41.0
18	38.49790131878804	40.0	38.0	41.0	34.0	41.0
19	38.36445408967282	40.0	37.0	41.0	34.0	41.0
20	38.39499742319482	40.0	37.0	41.0	34.0	41.0
21	38.3356340877067	40.0	37.0	41.0	34.0	41.0
22	38.401269635106516	40.0	37.0	41.0	34.0	41.0
23	38.38624224351852	40.0	37.0	41.0	34.0	41.0
24	38.36515116760554	40.0	37.0	41.0	34.0	41.0
25	38.121570093569304	40.0	37.0	41.0	34.0	41.0
26	38.14533032259218	40.0	37.0	41.0	34.0	41.0
27	38.13265780325124	40.0	37.0	41.0	34.0	41.0
28	38.07389919776458	40.0	37.0	41.0	34.0	41.0
29	38.0495401966713	40.0	37.0	41.0	34.0	41.0
30	37.877951782464024	40.0	36.0	41.0	33.0	41.0
31	37.86280672173781	40.0	36.0	41.0	33.0	41.0
32	37.77050198548052	40.0	36.0	41.0	33.0	41.0
33	37.68931028011213	40.0	36.0	41.0	33.0	41.0
34	37.59429915367589	40.0	36.0	41.0	33.0	41.0
35	37.5433826749323	40.0	36.0	41.0	33.0	41.0
36	37.45348792178428	40.0	36.0	41.0	33.0	41.0
37	37.380063869020844	40.0	36.0	41.0	33.0	41.0
38	37.27367756272957	40.0	36.0	41.0	33.0	41.0
39	37.18573850044834	40.0	35.0	41.0	32.0	41.0
40	37.11478401010465	40.0	35.0	41.0	32.0	41.0
41	37.02856381093102	40.0	35.0	41.0	32.0	41.0
42	36.98999067583791	40.0	35.0	41.0	32.0	41.0
43	36.92250072239914	40.0	35.0	41.0	32.0	41.0
44	36.88312177713167	40.0	35.0	41.0	32.0	41.0
45	36.87651592108124	39.0	35.0	41.0	32.0	41.0
46	36.76779708478434	39.0	35.0	41.0	31.0	41.0
47	36.70511220273648	39.0	35.0	41.0	31.0	41.0
48	36.628028490826274	39.0	35.0	41.0	31.0	41.0
49	36.575765519665644	39.0	35.0	41.0	31.0	41.0
50	36.463279781463086	39.0	35.0	41.0	31.0	41.0
51	35.50539639604751	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	24.0
10	27.0
11	25.0
12	16.0
13	21.0
14	17.0
15	19.0
16	55.0
17	102.0
18	158.0
19	320.0
20	616.0
21	1069.0
22	1619.0
23	2176.0
24	2995.0
25	4143.0
26	5043.0
27	5927.0
28	6163.0
29	6884.0
30	8181.0
31	10562.0
32	14085.0
33	21279.0
34	36809.0
35	50106.0
36	49089.0
37	76664.0
38	128794.0
39	238158.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71133228275149	19.16159994280386	22.30634489867049	12.820722875774159
2	33.232594649182126	24.847551439287194	24.94109095675436	16.978762954776325
3	32.708445665158315	24.916514491177793	26.158892063142154	16.216147780521737
4	28.55383139650925	25.48147530288632	26.827669823377132	19.137023477227295
5	22.718484778975654	37.272816641693005	22.195229484609175	17.813469094722166
6	24.310146058679663	37.326140124580334	24.099831092654764	14.263882724085233
7	79.60615096801484	6.187758239073899	9.897910851477716	4.308179941433537
8	81.04916186804971	6.652178964332846	8.376106313321637	3.9225528542958172
9	75.45704778558597	7.861341070699789	9.892101868705074	6.78950927500916
10	39.37641314677065	26.944594220211087	18.26820818202671	15.41078445099155
11	30.39989633199975	26.599481064205644	24.517184162627686	18.48343844116692
12	26.98406551340981	23.2408463836848	29.40179393303881	20.373294169866572
13	24.828039215102162	25.14544799173039	30.43996341830336	19.586549374864084
14	21.118184499250788	29.04729703563141	28.991292483772085	20.843225981345718
15	20.582119653129254	26.615567478037576	32.90982373460992	19.892489134223247
16	22.987485365831862	25.38287154402762	30.723709884505507	20.905933205635012
17	22.708952089297473	25.50843494088243	28.83802470754006	22.944588262280043
18	23.256932797516736	25.702514544799172	29.685391450964737	21.355161206719355
19	24.649301283636245	27.388906928180116	27.016238341073677	20.94555344710996
20	26.03288182145868	25.983282045476884	28.252062188884285	19.731773944180144
21	24.788716870179663	27.374607893662844	28.063195774635304	19.773479461522193
22	23.382496194371544	25.040290508717945	28.531191258523563	23.046022038386948
23	23.14492369379809	27.321284410775515	28.091346998841182	21.44244489658521
24	22.336730347019692	26.53126275369615	29.58812822659203	21.54387867269212
25	22.247510329562957	27.301772186590483	28.2011218784165	22.24959560543006
26	21.38107820678191	27.99348798136359	28.600899051795274	22.024534760059222
27	22.52380938195402	28.19054655080477	27.693059308224626	21.592584759016585
28	21.136803033778488	28.120689809256838	29.448414743496176	21.294092413468498
29	22.44173888175592	27.059135444625497	28.943926931933618	21.555198741684965
30	22.007852553122405	28.14243625758519	28.85396217309577	20.995749016196637
31	23.090408624700988	27.367011531575546	27.166676100057497	22.375903743665976
32	23.88623926455299	28.751932603883972	26.75930256459142	20.602525566971615
33	23.70437341928642	28.207079809465363	26.651612960883202	21.436933810365012
34	21.972700759934106	28.16194848177022	27.513874531930043	22.351476226365634
35	22.098413105065134	28.745378879730225	26.68572211613795	22.470485899066688
36	22.9573978140351	29.62685477840965	26.550328133052513	20.865419274502734
37	22.59545350281661	28.883751828340092	27.704677273769914	20.816117395073384
38	22.561493295838087	28.371816602966454	27.10635204818775	21.960338053007714
39	23.075215900526384	28.027746084894563	26.67648732301221	22.220550691566846
40	22.81708853783435	27.629607342554223	27.78927989466378	21.764024224947644
41	20.867206653817398	27.98306160202808	28.07600532639036	23.073726417764167
42	22.041514863548485	28.10475234370113	27.66848284264806	22.185249950102328
43	22.796086830887106	27.30624063487713	27.286877358968326	22.610795175267437
44	22.164992984536187	27.012216737615695	28.0707921367226	22.75199814112551
45	21.57649834518465	27.37579947987262	27.175761944907013	23.87194023003572
46	22.294726933125204	28.76414636253415	27.038580582506917	21.902546121833733
47	22.014704173828598	28.38194508574952	28.153756326578034	21.449594413843847
48	22.150693950018916	27.013855168654132	28.436162258294186	22.399288623032763
49	21.994149311710014	26.171105821792324	29.381536967472677	22.453207899024985
50	21.14186727517002	27.155356031064652	28.5031889825939	23.199587711171418
51	20.773369239797788	26.97646915132251	27.375650531596396	24.874511077283305
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	385.0
1	791.5
2	1198.0
3	3241.5
4	5285.0
5	3541.5
6	1798.0
7	1709.5
8	1621.0
9	1674.5
10	1728.0
11	1684.5
12	1641.0
13	1607.5
14	1574.0
15	1710.0
16	1846.0
17	1758.0
18	1670.0
19	1850.0
20	2030.0
21	2421.5
22	2813.0
23	3365.0
24	3917.0
25	4294.0
26	5273.5
27	5876.0
28	7600.0
29	9324.0
30	10615.0
31	11906.0
32	13725.0
33	15544.0
34	17319.5
35	19095.0
36	21820.5
37	24546.0
38	25887.0
39	27228.0
40	30674.0
41	34120.0
42	37955.5
43	41791.0
44	49539.0
45	57287.0
46	57970.5
47	58654.0
48	57225.5
49	55797.0
50	52142.5
51	48488.0
52	44798.5
53	41109.0
54	38390.0
55	35671.0
56	34131.0
57	32591.0
58	30943.5
59	29296.0
60	27428.0
61	25560.0
62	23622.0
63	21684.0
64	18461.5
65	15239.0
66	13084.5
67	10930.0
68	8617.5
69	6305.0
70	5353.0
71	4401.0
72	3590.5
73	2780.0
74	2286.0
75	1453.0
76	1114.0
77	827.0
78	540.0
79	385.5
80	231.0
81	193.0
82	155.0
83	107.5
84	60.0
85	45.0
86	30.0
87	36.0
88	42.0
89	24.5
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	671374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.017198673962834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13187405138366	20.930649200742643
2	9.205622495646736	5.342427537473659
3	3.7885961212409764	3.298033390363632
4	2.168425244924949	2.51686504566495
5	1.4454679330549516	2.0971715095148973
6	1.0812910306730938	1.882562199684913
7	0.8535570906393265	1.7337484975055728
8	0.7012160129343245	1.6277859488543507
9	0.5821670062963484	1.5203570114814582
>10	7.703268856112529	50.5924063904677
>50	0.2988730539511959	5.168513002477403
>100	0.03703313582986471	1.9639411028166176
>500	0.0	0.0
>1k	0.0026079673119623037	1.3255391629522149
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2711	0.4037987768367557	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	1845	0.27480956962885067	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	1599	0.23816829367833725	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	1524	0.22699717296171731	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	1079	0.16071519004310564	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.978965524431986E-4	0.0	0.0	0.07402729328213485	0.0
2	2.978965524431986E-4	0.0	0.0	0.38100969057485096	0.0
3	2.978965524431986E-4	0.0	0.0	0.509254156401648	0.0
4	2.978965524431986E-4	0.0	0.0	0.7262717948565182	0.0
5	2.978965524431986E-4	0.0	0.0	1.4698215897547418	0.0
6	2.978965524431986E-4	0.0	0.0	1.966713039229997	0.0
7	2.978965524431986E-4	0.0	0.0	2.389279298870674	0.0
8	2.978965524431986E-4	0.0	0.0	3.0325869038717617	0.0
9	2.978965524431986E-4	0.0	0.0	3.303374870042629	0.0
10	2.978965524431986E-4	0.0	0.0	4.1225903892614255	0.0
11	2.978965524431986E-4	0.0	0.0	4.853926425509478	0.0
12	2.978965524431986E-4	0.0	0.0	5.690122048217536	0.0
13	2.978965524431986E-4	0.0	0.0	5.960761066112182	0.0
14	2.978965524431986E-4	0.0	0.0	6.0782812560510235	0.0
15	2.978965524431986E-4	0.0	0.0	6.304384739355411	0.0
16	2.978965524431986E-4	0.0	0.0	6.6894160333882455	0.0
17	2.978965524431986E-4	0.0	0.0	7.135367172395714	0.0
18	2.978965524431986E-4	0.0	0.0	7.611554811476167	0.0
19	4.4684482866479787E-4	0.0	0.0	7.9620301054255895	0.0
20	4.4684482866479787E-4	0.0	0.0	8.26945934754697	0.0
21	4.4684482866479787E-4	0.0	0.0	8.628424693241024	0.0
22	5.957931048863972E-4	0.0	0.0	9.036542970088208	0.0
23	5.957931048863972E-4	0.0	0.0	9.464471367672862	0.0
24	5.957931048863972E-4	0.0	0.0	9.801690265038562	0.0
25	5.957931048863972E-4	0.0	0.0	10.091990455394459	0.0
26	5.957931048863972E-4	0.0	0.0	10.368587404337969	0.0
27	7.447413811079964E-4	0.0	0.0	10.649652801568127	0.0
28	7.447413811079964E-4	0.0	0.0	10.945761974696667	0.0
29	7.447413811079964E-4	0.0	0.0	11.260489682352906	0.0
30	7.447413811079964E-4	0.0	0.0	11.642095166032643	0.0
31	7.447413811079964E-4	0.0	0.0	11.978271425464794	0.0
32	7.447413811079964E-4	0.0	0.0	12.3141497883445	0.0
33	8.936896573295957E-4	0.0	0.0	12.631409616696507	0.0
34	8.936896573295957E-4	0.0	0.0	12.935264100188569	0.0
35	8.936896573295957E-4	0.0	0.0	13.251630238883246	0.0
36	8.936896573295957E-4	0.0	0.0	13.556974205137523	0.0
37	0.001042637933551195	0.0	0.0	13.8843625162726	0.0
38	0.001042637933551195	0.0	0.0	14.206537637739919	0.0
39	0.001042637933551195	0.0	0.0	14.545394966144057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	30	2.1641463E-6	45.000004	1
TCGCCGT	20	7.031188E-4	45.0	39
CGTAATG	20	7.031188E-4	45.0	43
GCGTAAG	75	0.0	42.0	1
TACGAAT	65	0.0	41.538464	12
CGAATAT	65	0.0	41.538464	14
CGTAAGG	190	0.0	40.263157	2
ATAACGG	45	1.9263098E-8	40.0	2
CGTTTTT	1495	0.0	39.73244	1
CGGAAAT	40	3.4553523E-7	39.375	23
TTACGCG	35	6.244407E-6	38.57143	1
GCTACGA	70	0.0	38.57143	10
CGTTATT	195	0.0	38.076923	1
ATCGATT	30	1.1394159E-4	37.500004	43
GCGTTAG	115	0.0	37.173916	1
CGACAGG	80	0.0	36.5625	2
TACGGGA	230	0.0	36.195656	4
GTACGGG	255	0.0	36.17647	3
ACGGGGT	280	0.0	36.160717	5
TTTCGCG	25	0.0021064861	36.0	1
>>END_MODULE