##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936006.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356436
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.026753751024025	33.0	31.0	34.0	30.0	34.0
2	32.17243207756792	33.0	31.0	34.0	30.0	34.0
3	31.996641753358247	33.0	31.0	34.0	30.0	34.0
4	35.73981303796474	37.0	35.0	37.0	33.0	37.0
5	31.953192157918952	37.0	35.0	37.0	0.0	37.0
6	33.83489322066234	37.0	35.0	37.0	17.0	37.0
7	35.5469789807988	37.0	35.0	37.0	32.0	37.0
8	35.83981977129134	37.0	35.0	37.0	35.0	37.0
9	37.83810277300834	39.0	38.0	39.0	35.0	39.0
10	37.47064269602397	39.0	37.0	39.0	35.0	39.0
11	37.398051824170395	39.0	37.0	39.0	34.0	39.0
12	37.301762448237554	39.0	37.0	39.0	35.0	39.0
13	37.213062092493466	39.0	37.0	39.0	34.0	39.0
14	38.43753436802119	40.0	38.0	41.0	34.0	41.0
15	38.62849431595013	40.0	38.0	41.0	35.0	41.0
16	38.577405200372574	40.0	38.0	41.0	35.0	41.0
17	38.59352029536859	40.0	38.0	41.0	35.0	41.0
18	38.37245957198487	40.0	37.0	41.0	35.0	41.0
19	38.101148593295854	40.0	37.0	41.0	34.0	41.0
20	38.0049433839455	40.0	35.0	41.0	34.0	41.0
21	37.89091449797439	40.0	35.0	41.0	34.0	41.0
22	37.94592016519095	40.0	35.0	41.0	34.0	41.0
23	37.95757162576171	40.0	35.0	41.0	34.0	41.0
24	37.91361141972191	40.0	35.0	41.0	34.0	41.0
25	37.65846323042566	39.0	35.0	41.0	34.0	41.0
26	37.692545085232695	39.0	35.0	41.0	34.0	41.0
27	37.673854492812175	39.0	35.0	41.0	34.0	41.0
28	37.582926528184586	39.0	35.0	41.0	34.0	41.0
29	37.54205523572254	39.0	35.0	41.0	34.0	41.0
30	37.306618860047806	39.0	35.0	41.0	33.0	41.0
31	37.12748992806563	39.0	35.0	41.0	33.0	41.0
32	36.83173977937133	39.0	35.0	41.0	32.0	41.0
33	36.457302292697705	39.0	35.0	41.0	31.0	41.0
34	36.13119606324838	39.0	35.0	41.0	30.0	41.0
35	35.976722328833226	39.0	35.0	41.0	29.0	41.0
36	35.795239538093796	39.0	35.0	41.0	27.0	41.0
37	35.68383103839118	39.0	35.0	41.0	25.0	41.0
38	35.5494871449573	39.0	35.0	41.0	25.0	41.0
39	35.46603317285572	39.0	35.0	41.0	24.0	41.0
40	35.36724124386987	39.0	35.0	41.0	23.0	41.0
41	35.276874389792276	39.0	35.0	41.0	23.0	41.0
42	35.24150478627299	39.0	35.0	41.0	23.0	41.0
43	35.12037785184437	39.0	35.0	41.0	23.0	41.0
44	35.036124297209035	39.0	35.0	41.0	22.0	41.0
45	35.04551167671055	39.0	35.0	41.0	23.0	41.0
46	34.969568169320716	39.0	35.0	41.0	22.0	41.0
47	34.907178287266156	38.0	35.0	41.0	22.0	41.0
48	34.876965289701374	38.0	35.0	41.0	22.0	41.0
49	34.86824002064887	38.0	35.0	41.0	22.0	41.0
50	34.74988497233725	38.0	35.0	40.0	20.0	41.0
51	33.884955503933384	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	7.0
11	13.0
12	7.0
13	5.0
14	8.0
15	13.0
16	25.0
17	55.0
18	99.0
19	205.0
20	359.0
21	608.0
22	1069.0
23	1674.0
24	2935.0
25	4956.0
26	6522.0
27	7319.0
28	6818.0
29	6105.0
30	5959.0
31	6729.0
32	8347.0
33	11844.0
34	20572.0
35	32063.0
36	25257.0
37	36290.0
38	61875.0
39	108592.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.136439641338136	18.018381981618017	22.80942441279781	21.035753964246034
2	39.03786373991404	21.782872661571783	23.517265371623516	15.661998226890661
3	27.145125632652146	21.68860608917169	35.480703408185484	15.685564869990687
4	25.724954830600726	22.560291328597557	34.55571266650956	17.15904117429216
5	19.423122243544423	34.93951228270994	29.66114533885466	15.976220134890976
6	21.36091752797136	30.81058030053081	33.78530788135878	14.043194290139043
7	71.53233680099653	5.025586641080026	18.982369906518983	4.45970665140446
8	73.05154361512305	4.416781694329417	17.717346171542715	4.814328519004815
9	67.91036820074291	6.5369379075065375	19.986196680469988	5.566497211280566
10	35.32415356473532	25.62086882357562	26.42690412865143	12.628073483037628
11	25.941824058175943	21.6992671896217	35.55673388771056	16.802174864491803
12	24.237450762549237	20.243185312370244	38.23715898506323	17.28220494001728
13	20.85984580682086	23.97765657789898	39.169444163889175	15.993053451390992
14	16.493002951441493	27.76178612710276	37.29056548721229	18.454645434243456
15	15.027382194840028	24.31740901592432	44.12881976006913	16.526389029166527
16	17.16998283001717	22.248594418072248	41.34206421349134	19.23935853841924
17	17.17222727221717	22.16975838579717	37.2984210349123	23.35959330707336
18	18.28042060846828	22.45901087432246	39.38406894926438	19.876499567944876
19	19.384686170869383	24.164786946324167	37.48639306916249	18.96413381364396
20	19.994332783444992	24.867577910199866	37.10455733988711	18.033531966468033
21	19.243566867544242	25.43037179185043	37.402507041937405	17.923554298667923
22	17.655904566317655	23.897698324523898	35.81933362511082	22.62706348404763
23	16.117619993491118	25.277749722250277	36.70981606796171	21.894814216296897
24	17.961990371342964	23.175829601948177	38.49050039838849	20.371679628320372
25	16.563983436016564	25.414941251725416	36.38605528061139	21.635020031646633
26	15.63927324961564	25.65846323042566	37.857287142712856	20.844976377245843
27	17.896901547542896	25.97184347260097	37.96025092863796	18.17100405121817
28	15.51975670246552	24.154686956424154	40.49085950914049	19.834696831969833
29	16.324389231166325	22.874793791872875	39.646107576114645	21.154709400846155
30	19.185211370344184	25.062844381600062	36.864402024486864	18.887542223568886
31	19.007339325994007	26.570267874176572	34.19379636175919	20.22859643807023
32	19.227855772144228	26.85671480995186	33.71544961788371	20.199979800020202
33	21.15078162699615	26.753751024026755	32.16594283405716	19.92952451491993
34	17.914015419317913	26.538284572826537	34.221290778709225	21.326409229146325
35	18.122748543918124	26.473756859576476	33.02416142028303	22.379333176222378
36	21.427128572871425	26.455520766701458	33.69468852753369	18.42266213289342
37	18.984614348718985	27.36423930242736	34.44292944595944	19.20821690289421
38	17.6037212851676	28.65311023577865	31.27293539373127	22.47023308532247
39	19.546566564544545	27.63188903477763	32.002659664007005	20.81888473667082
40	20.145832631945147	27.22873110460223	32.416759249907415	20.20867701354521
41	18.315209462568316	27.50620027157751	31.75969879585676	22.41889146999742
42	21.583958971596584	25.750485360625753	31.934484732181932	20.73107093559573
43	21.88864200024689	24.45011166099945	32.522528588582524	21.13871775017114
44	18.72874793791873	25.764513124375764	32.075042924957074	23.431696012748432
45	18.673197993468673	26.304582028751305	30.928132960755928	24.094087017024094
46	20.614640496470614	27.303358807752304	32.11572343983212	19.966277255944966
47	18.27032061856827	26.92909807090193	34.05295761370905	20.747623696820746
48	18.92176996711892	24.774153003624775	33.343152767958344	22.96092426129796
49	20.142746523920145	23.235307320248236	34.31022680088431	22.311719354947314
50	18.85948669606886	23.674376325623676	33.388883277783385	24.077253700524075
51	17.778507221492777	24.945852831924945	30.65823878620566	26.61740116037662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	356.0
1	574.5
2	793.0
3	7172.5
4	13552.0
5	8706.5
6	3861.0
7	3755.5
8	3650.0
9	3687.5
10	3725.0
11	3674.5
12	3624.0
13	3479.0
14	3334.0
15	3157.5
16	2981.0
17	2748.5
18	2516.0
19	2446.5
20	2377.0
21	2301.0
22	2225.0
23	2365.0
24	2505.0
25	2652.5
26	2878.0
27	2956.0
28	3536.0
29	4116.0
30	5062.0
31	6008.0
32	6725.0
33	7442.0
34	8337.5
35	9233.0
36	10031.5
37	10830.0
38	12291.0
39	13752.0
40	16373.0
41	18994.0
42	22244.0
43	25494.0
44	29408.5
45	33323.0
46	36597.5
47	39872.0
48	38649.0
49	37426.0
50	31633.5
51	25841.0
52	21916.0
53	17991.0
54	15504.0
55	13017.0
56	11941.5
57	10866.0
58	9722.5
59	8579.0
60	7935.0
61	7291.0
62	6222.0
63	5153.0
64	4377.0
65	3601.0
66	2936.5
67	2272.0
68	1771.5
69	1271.0
70	1092.0
71	913.0
72	800.0
73	687.0
74	666.0
75	442.5
76	240.0
77	210.0
78	180.0
79	125.5
80	71.0
81	65.5
82	60.0
83	33.0
84	6.0
85	3.0
86	0.0
87	0.5
88	1.0
89	3.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	356436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.62889808140913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09912357884309	30.157244123905315
2	10.272927944626845	8.142096290305542
3	3.878027149673994	4.6104582801410485
4	1.9679852810642957	3.119563525160411
5	1.133042747762909	2.245061778648804
6	0.7932107614620015	1.8860441053832757
7	0.6103279158494168	1.6930635941404777
8	0.48104329163720244	1.525057246162901
9	0.37194277960374633	1.3265714254529597
>10	4.219239367415335	35.781032351696
>50	0.13608096203161585	3.4330004364138382
>100	0.032060959640956614	2.1124853413313875
>500	0.0028498630791961434	0.9466938451596021
>1k	0.0014249315395980717	0.6719747543930369
>5k	7.124657697990358E-4	2.3496529017054004
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8322	2.334780998552335	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1291	0.3621968600253622	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1089	0.3055246944753055	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	987	0.27690805642527694	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	872	0.24464419980024465	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	857	0.24043587067524044	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	637	0.17871371017517873	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	408	0.11446655220011445	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25334141332525334	0.0
2	0.0	0.0	0.0	1.265023734976265	0.0
3	0.0	0.0	0.0	1.7035316298017036	0.0
4	0.0	0.0	0.0	2.2343422101022345	0.0
5	0.0	0.0	0.0	3.636838029828637	0.0
6	0.0	0.0	0.0	4.56996487447957	0.0
7	0.0	0.0	0.0	5.517400038155517	0.0
8	0.0	0.0	0.0	7.06999293000707	0.0
9	0.0	0.0	0.0	7.633909032757634	0.0
10	0.0	0.0	0.0	9.036404852484036	0.0
11	0.0	0.0	0.0	11.14000830443614	0.0
12	0.0	0.0	0.0	12.71027617861271	0.0
13	0.0	0.0	0.0	13.29018393203829	0.0
14	0.0	0.0	0.0	13.507053159613507	0.0
15	0.0	0.0	0.0	13.889169444163889	0.0
16	0.0	0.0	0.0	14.77937133173978	0.0
17	0.0	0.0	0.0	15.890931331290892	0.0
18	0.0	0.0	0.0	17.114152330292114	0.0
19	0.0	0.0	0.0	17.833496055392832	0.0
20	0.0	0.0	0.0	18.508792602318508	0.0
21	0.0	0.0	0.0	19.296591814519296	0.0
22	0.0	0.0	0.0	20.17921870967018	0.0
23	0.0	0.0	0.0	21.094390016721093	0.0
24	0.0	0.0	0.0	21.72816438294673	0.0
25	0.0	0.0	0.0	22.31901379209732	0.0
26	0.0	0.0	0.0	22.847018819647847	0.0
27	0.0	0.0	0.0	23.39017383204839	0.0
28	5.611105500005611E-4	0.0	0.0	23.92014274652392	0.0
29	5.611105500005611E-4	0.0	0.0	24.457967208699458	0.0
30	5.611105500005611E-4	0.0	0.0	25.117833215500116	0.0
31	5.611105500005611E-4	0.0	0.0	25.70868262465071	0.0
32	5.611105500005611E-4	0.0	0.0	26.244823755176245	0.0
33	5.611105500005611E-4	0.0	0.0	26.76132601645176	0.0
34	5.611105500005611E-4	0.0	0.0	27.252578302977252	0.0
35	5.611105500005611E-4	0.0	0.0	27.81256663187781	0.0
36	5.611105500005611E-4	0.0	0.0	28.363857747253363	0.0
37	5.611105500005611E-4	0.0	0.0	28.894668327553894	0.0
38	5.611105500005611E-4	0.0	0.0	29.392373385404394	0.0
39	5.611105500005611E-4	0.0	0.0	29.92009785767992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAACG	30	2.1612723E-6	45.000004	1
ATTGAGC	30	2.1612723E-6	45.000004	12
CGTGGTT	20	7.0267305E-4	45.0	19
ACCGGCG	25	3.8853723E-5	45.0	20
ACACGCG	20	7.0267305E-4	45.0	34
TTGTGCG	20	7.0267305E-4	45.0	1
TTTACGG	40	6.7921064E-9	45.0	2
TTTACCG	20	7.0267305E-4	45.0	13
CATGCGG	25	3.8853723E-5	45.0	2
GTTTACG	25	3.8853723E-5	45.0	1
GACCGAG	20	7.0267305E-4	45.0	1
TGACGCA	20	7.0267305E-4	45.0	22
ACGGGCC	20	7.0267305E-4	45.0	5
ATCCGGG	40	6.7921064E-9	45.0	3
TAACCGG	25	3.8853723E-5	45.0	2
GGGCGGA	35	1.208773E-7	45.0	7
AGCGATT	20	7.0267305E-4	45.0	21
TAGGGCG	45	3.8380676E-10	45.0	5
GCGATGC	25	3.8853723E-5	45.0	9
CTCGCGG	20	7.0267305E-4	45.0	3
>>END_MODULE