##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936005.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 525375 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.004166547704024 33.0 31.0 34.0 30.0 34.0 2 32.12375350939805 33.0 31.0 34.0 30.0 34.0 3 32.009195336664284 33.0 31.0 34.0 30.0 34.0 4 35.70157696883179 37.0 35.0 37.0 33.0 37.0 5 31.982509635974303 37.0 35.0 37.0 0.0 37.0 6 33.817600761360936 37.0 35.0 37.0 17.0 37.0 7 35.522938853200095 37.0 35.0 37.0 32.0 37.0 8 35.8020613847252 37.0 35.0 37.0 35.0 37.0 9 37.77399191054009 39.0 38.0 39.0 35.0 39.0 10 37.21029169640733 39.0 37.0 39.0 34.0 39.0 11 37.27692410183202 39.0 37.0 39.0 34.0 39.0 12 37.260788960266474 39.0 37.0 39.0 34.0 39.0 13 37.21300214132762 39.0 37.0 39.0 34.0 39.0 14 38.436594813228645 40.0 38.0 41.0 34.0 41.0 15 38.59575350939805 40.0 38.0 41.0 34.0 41.0 16 38.550696169402805 40.0 38.0 41.0 34.0 41.0 17 38.56673994765644 40.0 38.0 41.0 34.0 41.0 18 38.400574827504165 40.0 38.0 41.0 34.0 41.0 19 38.13798715203426 40.0 37.0 41.0 34.0 41.0 20 38.14326528669998 40.0 36.0 41.0 34.0 41.0 21 38.04572543421366 40.0 36.0 41.0 34.0 41.0 22 38.09677658815132 40.0 36.0 41.0 34.0 41.0 23 38.12129050678087 40.0 36.0 41.0 34.0 41.0 24 38.0709664525339 40.0 36.0 41.0 34.0 41.0 25 37.81846300261718 40.0 35.0 41.0 34.0 41.0 26 37.87763978110873 40.0 35.0 41.0 34.0 41.0 27 37.847960028551036 40.0 35.0 41.0 34.0 41.0 28 37.79644825124911 40.0 35.0 41.0 34.0 41.0 29 37.747351891506064 40.0 35.0 41.0 34.0 41.0 30 37.525961456102785 40.0 35.0 41.0 33.0 41.0 31 37.39487604092315 40.0 35.0 41.0 33.0 41.0 32 37.168504401617895 40.0 35.0 41.0 33.0 41.0 33 36.90954841779681 40.0 35.0 41.0 31.0 41.0 34 36.66970830359267 40.0 35.0 41.0 31.0 41.0 35 36.538788484415896 40.0 35.0 41.0 30.0 41.0 36 36.39536331192006 40.0 35.0 41.0 30.0 41.0 37 36.31617416131335 40.0 35.0 41.0 30.0 41.0 38 36.206610516297886 40.0 35.0 41.0 29.0 41.0 39 36.14086509635974 40.0 35.0 41.0 29.0 41.0 40 36.077186771353794 39.0 35.0 41.0 28.0 41.0 41 35.94639067332857 39.0 35.0 41.0 27.0 41.0 42 35.914107066381156 39.0 35.0 41.0 27.0 41.0 43 35.83189721627409 39.0 35.0 41.0 27.0 41.0 44 35.744576730906495 39.0 35.0 41.0 26.0 41.0 45 35.73083987627885 39.0 35.0 41.0 26.0 41.0 46 35.60324910778016 39.0 35.0 41.0 26.0 41.0 47 35.51469521770164 39.0 35.0 41.0 25.0 41.0 48 35.47517868189389 39.0 35.0 41.0 25.0 41.0 49 35.442904591958126 39.0 35.0 41.0 26.0 41.0 50 35.31467808708066 39.0 35.0 41.0 25.0 41.0 51 34.307304306447776 37.0 34.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 6.0 9 11.0 10 11.0 11 11.0 12 6.0 13 18.0 14 11.0 15 17.0 16 32.0 17 72.0 18 179.0 19 296.0 20 512.0 21 931.0 22 1431.0 23 2275.0 24 3721.0 25 6191.0 26 8050.0 27 8627.0 28 8068.0 29 7705.0 30 8406.0 31 9464.0 32 12037.0 33 17443.0 34 28629.0 35 40726.0 36 37612.0 37 57430.0 38 97855.0 39 167453.0 40 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6733285748275 18.608803235783963 21.867047347133 16.85082084225553 2 37.93861527480371 22.48831786818939 22.942850344991673 16.630216512015227 3 29.61732096121818 22.4538662859862 31.290030930287887 16.638781822507735 4 26.327480371163453 24.657054484891745 31.520913633119203 17.494551510825602 5 20.63002617178206 36.44901261004045 27.471615512729002 15.449345705448488 6 22.777444682369737 33.94051867713538 30.239733523673568 13.04230311682132 7 75.51234832262669 5.741613133476089 14.508684273138234 4.237354270758982 8 77.37920532952653 4.7088270283131095 13.533571258624791 4.37839638353557 9 72.24496788008565 6.436735665001189 15.766833214370687 5.5514632405424695 10 35.97582679038781 22.281037354270758 26.209469426600045 15.533666428741375 11 28.755079704972637 25.049726385914823 27.860480609088746 18.334713300023793 12 24.577111586961696 22.568831786818937 33.95060670949322 18.90344991672615 13 22.36212229359981 24.95093980490126 35.841827266238404 16.84511063526053 14 20.055960028551034 27.565072567213893 34.161503687842014 18.217463716393052 15 19.341232453009756 25.60647156792767 37.779110159409946 17.27318581965263 16 21.62855103497502 23.448203664049487 35.861812990720914 19.06143231025458 17 21.53547466095646 24.160076136093267 32.40599571734475 21.898453485605522 18 22.30996906971211 23.60961218177492 35.383678325005945 18.69674042350702 19 21.149655008327386 27.39167261479895 32.68998334522959 18.768689031644065 20 22.40837497025934 25.840209374256485 32.79448013323816 18.956935522246017 21 20.925434213656914 26.921722579110156 33.761789198191764 18.39105400904116 22 19.982488698548657 25.15422317392339 32.719486081370455 22.143802046157507 23 18.746228884130385 27.30525814894123 32.378396383535566 21.570116583392814 24 19.79157744468237 25.616369260052345 34.16492981203902 20.427123483226268 25 18.62498215560314 27.016131334760885 32.85805377111587 21.500832738520103 26 18.49593147751606 27.617987152034264 33.9684986914109 19.91758267903878 27 19.662907447061624 27.63949559838211 32.76117059243398 19.936426362122294 28 18.25686414465858 26.855103497501787 33.98734237449441 20.90068998334523 29 19.632833690221272 25.77968118010945 33.40832738520105 21.179157744468238 30 19.965358077563643 27.189340946942657 32.09269569355222 20.75260528194147 31 20.097644539614564 28.144848917439923 29.638829407566025 22.118677135379492 32 20.190530573399954 28.996050440161792 30.124387342374494 20.689031644063764 33 20.179300499643112 28.88717582679039 28.619747799191053 22.313775874375448 34 18.900594813228647 28.312157982393526 29.515679276707115 23.27156792767071 35 19.070568641446588 30.109921484653817 28.719295741137284 22.100214132762314 36 20.556935522246015 29.67175826790388 28.0997382821794 21.67156792767071 37 18.59072091363312 31.456768974541994 29.248631929574113 20.703878182250772 38 17.899214846538186 31.41184867951463 27.59019747799191 23.09873899595527 39 19.18934094694266 29.845919581251483 28.943897216274088 22.020842255531765 40 20.442541042112776 28.490697121103974 29.443921008803237 21.622840827980014 41 18.855103497501783 29.923959076849872 28.262098501070664 22.95883892457768 42 20.19738282179396 29.565929098263144 28.610801808232218 21.625886271710684 43 19.955270045205804 28.202712348322628 29.38320247442303 22.45881513204854 44 19.267190102307875 28.480609088746135 28.446538187009278 23.805662621936712 45 19.550416369260052 27.974684748988814 28.31006423982869 24.164834641922436 46 20.97454199381394 29.099214846538185 27.90482988341661 22.021413276231264 47 18.879657387580302 28.847204377825364 29.775113014513444 22.498025220080894 48 19.217320961218178 28.40028551034975 29.207899119676423 23.17449440875565 49 20.033499881037354 26.470235546038545 30.369355222460147 23.126909350463954 50 19.306780870806566 26.789436117059246 29.464287413752082 24.43949559838211 51 18.994051867713537 27.008137044967878 27.803378539138713 26.19443254817987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 370.0 1 554.0 2 738.0 3 5648.0 4 10558.0 5 7151.0 6 3744.0 7 3714.0 8 3684.0 9 3670.5 10 3657.0 11 3652.5 12 3648.0 13 3572.5 14 3497.0 15 3361.0 16 3225.0 17 3128.0 18 3031.0 19 2837.0 20 2643.0 21 2836.0 22 3029.0 23 3087.0 24 3145.0 25 3230.0 26 3912.5 27 4510.0 28 5224.5 29 5939.0 30 7138.0 31 8337.0 32 10055.5 33 11774.0 34 12510.0 35 13246.0 36 15191.0 37 17136.0 38 19300.5 39 21465.0 40 24137.0 41 26809.0 42 31491.0 43 36173.0 44 42199.5 45 48226.0 46 52024.0 47 55822.0 48 54786.5 49 53751.0 50 48377.0 51 43003.0 52 38304.5 53 33606.0 54 30362.5 55 27119.0 56 23606.0 57 20093.0 58 17624.0 59 15155.0 60 13508.5 61 11862.0 62 10137.5 63 8413.0 64 7036.0 65 5659.0 66 4594.5 67 3530.0 68 2863.0 69 2196.0 70 1873.5 71 1551.0 72 1164.0 73 777.0 74 612.5 75 336.5 76 225.0 77 163.5 78 102.0 79 98.5 80 95.0 81 60.5 82 26.0 83 19.5 84 13.0 85 10.0 86 7.0 87 12.5 88 18.0 89 10.0 90 2.0 91 1.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 525375.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.825316700661965 #Duplication Level Percentage of deduplicated Percentage of total 1 75.4907430878038 30.819334945278992 2 10.142666462134278 8.281551410116291 3 3.8217502406488477 4.680724917759609 4 2.0358261195367926 3.3245298431027708 5 1.2449826492389324 2.541340547100429 6 0.9413848181460762 2.3059400002805175 7 0.7274602913603033 2.078915774735815 8 0.560805944918247 1.831606424712114 9 0.4786469636065962 1.758682248934456 >10 4.4341043426686175 36.048420202849414 >50 0.09475753902128048 2.4756517227883803 >100 0.024042957661907862 1.8183905369462006 >500 0.0014142916271710508 0.4509408370094145 >1k 9.428610847807005E-4 0.4979018119263147 >5k 4.7143054239035025E-4 1.086068776459294 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5643 1.0740899357601714 No Hit GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC 1499 0.2853200095170117 No Hit CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC 1088 0.20709017368546276 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC 912 0.1735902926481085 No Hit CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT 789 0.15017844396859387 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 642 0.12219842969307639 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.9034023316678563E-4 0.0 0.0 0.1547466095645967 0.0 2 1.9034023316678563E-4 0.0 0.0 0.8118010944563407 0.0 3 1.9034023316678563E-4 0.0 0.0 1.1138710444920294 0.0 4 1.9034023316678563E-4 0.0 0.0 1.462384011420414 0.0 5 1.9034023316678563E-4 0.0 0.0 2.491363311920057 0.0 6 1.9034023316678563E-4 0.0 0.0 3.281655960028551 0.0 7 1.9034023316678563E-4 0.0 0.0 4.081846300261718 0.0 8 1.9034023316678563E-4 0.0 0.0 5.392909826314537 0.0 9 1.9034023316678563E-4 0.0 0.0 5.910635260528194 0.0 10 1.9034023316678563E-4 0.0 0.0 7.218272662384011 0.0 11 1.9034023316678563E-4 0.0 0.0 8.82017606471568 0.0 12 1.9034023316678563E-4 0.0 0.0 10.073756840352129 0.0 13 1.9034023316678563E-4 0.0 0.0 10.560266476326433 0.0 14 1.9034023316678563E-4 0.0 0.0 10.752129431358554 0.0 15 1.9034023316678563E-4 0.0 0.0 11.093599809659766 0.0 16 1.9034023316678563E-4 0.0 0.0 11.861622650487746 0.0 17 1.9034023316678563E-4 0.0 0.0 12.806852248394005 0.0 18 1.9034023316678563E-4 0.0 0.0 13.780252200808945 0.0 19 1.9034023316678563E-4 0.0 0.0 14.386105162978824 0.0 20 5.710206995003569E-4 0.0 0.0 14.932000951701166 0.0 21 5.710206995003569E-4 0.0 0.0 15.686699976207471 0.0 22 5.710206995003569E-4 0.0 0.0 16.464239828693792 0.0 23 5.710206995003569E-4 0.0 0.0 17.292029502736142 0.0 24 5.710206995003569E-4 0.0 0.0 17.928907922912206 0.0 25 5.710206995003569E-4 0.0 0.0 18.518201284796575 0.0 26 5.710206995003569E-4 0.0 0.0 19.074946466809422 0.0 27 5.710206995003569E-4 0.0 0.0 19.615322388769926 0.0 28 5.710206995003569E-4 0.0 0.0 20.17530335474661 0.0 29 5.710206995003569E-4 0.0 0.0 20.755460385438973 0.0 30 5.710206995003569E-4 0.0 0.0 21.405852962169877 0.0 31 5.710206995003569E-4 0.0 0.0 22.03226266952177 0.0 32 5.710206995003569E-4 0.0 0.0 22.62441113490364 0.0 33 5.710206995003569E-4 0.0 0.0 23.213323816321676 0.0 34 5.710206995003569E-4 0.0 0.0 23.759790625743516 0.0 35 7.613609326671425E-4 0.0 0.0 24.306638115631692 0.0 36 7.613609326671425E-4 0.0 0.0 24.8492981203902 0.0 37 7.613609326671425E-4 0.0 0.0 25.432881275279563 0.0 38 7.613609326671425E-4 0.0 0.0 25.969831073043064 0.0 39 7.613609326671425E-4 0.0 0.0 26.538377349512253 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACGA 25 3.8879036E-5 45.0 13 AGCGTTC 25 3.8879036E-5 45.0 20 CGCATGG 45 3.8380676E-10 45.0 2 CGCGAGG 35 1.2102464E-7 45.0 2 CGACAAT 20 7.029787E-4 45.0 20 AAACGCA 20 7.029787E-4 45.0 10 CCGTAGA 20 7.029787E-4 45.0 39 TTGCGGT 25 3.8879036E-5 45.0 3 AGCGAAG 20 7.029787E-4 45.0 1 CTACCGG 20 7.029787E-4 45.0 2 CGTACCA 20 7.029787E-4 45.0 41 TCGTACC 25 3.8879036E-5 45.0 40 TAGTGCG 35 1.2102464E-7 45.0 1 CGTCATA 90 0.0 45.0 38 TGAGACG 20 7.029787E-4 45.0 1 GCTAACG 30 2.163244E-6 44.999996 1 AACGGGC 115 0.0 43.043476 4 CGTTTTT 3505 0.0 43.009987 1 CGAAAGG 80 0.0 42.1875 2 CGTTATT 435 0.0 41.896553 1 >>END_MODULE