##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936004.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	960830
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11698947784728	33.0	31.0	34.0	30.0	34.0
2	32.251653258120584	34.0	31.0	34.0	30.0	34.0
3	32.12375029922047	33.0	31.0	34.0	30.0	34.0
4	35.79034584681994	37.0	35.0	37.0	35.0	37.0
5	32.0454971222797	37.0	35.0	37.0	0.0	37.0
6	33.876760717296506	37.0	35.0	37.0	17.0	37.0
7	35.576163317132064	37.0	35.0	37.0	32.0	37.0
8	35.84951135997002	37.0	35.0	37.0	35.0	37.0
9	37.836377923253856	39.0	38.0	39.0	35.0	39.0
10	37.391038997533386	39.0	37.0	39.0	34.0	39.0
11	37.349353163410804	39.0	37.0	39.0	34.0	39.0
12	37.39546537889117	39.0	37.0	39.0	35.0	39.0
13	37.40088048874411	39.0	37.0	39.0	35.0	39.0
14	38.68630871225919	40.0	38.0	41.0	35.0	41.0
15	38.812128055951625	40.0	38.0	41.0	35.0	41.0
16	38.733347210224494	40.0	38.0	41.0	35.0	41.0
17	38.730751537733	40.0	38.0	41.0	35.0	41.0
18	38.569954102182486	40.0	38.0	41.0	35.0	41.0
19	38.34484872454024	40.0	37.0	41.0	34.0	41.0
20	38.332602021169194	40.0	37.0	41.0	34.0	41.0
21	38.241643162682266	40.0	37.0	41.0	34.0	41.0
22	38.29889574638594	40.0	37.0	41.0	34.0	41.0
23	38.2957953019785	40.0	37.0	41.0	34.0	41.0
24	38.24942601708939	40.0	36.0	41.0	34.0	41.0
25	37.99311740890688	40.0	36.0	41.0	34.0	41.0
26	38.042215584442616	40.0	36.0	41.0	34.0	41.0
27	38.03901314488515	40.0	36.0	41.0	34.0	41.0
28	37.97223962615655	40.0	36.0	41.0	34.0	41.0
29	37.92082574440848	40.0	36.0	41.0	34.0	41.0
30	37.70547755586316	40.0	36.0	41.0	33.0	41.0
31	37.595758875139204	40.0	36.0	41.0	33.0	41.0
32	37.386321201461236	40.0	36.0	41.0	33.0	41.0
33	37.138497965300836	40.0	36.0	41.0	32.0	41.0
34	36.90485725882831	40.0	36.0	41.0	31.0	41.0
35	36.774204593944816	40.0	36.0	41.0	31.0	41.0
36	36.63724384126224	40.0	35.0	41.0	30.0	41.0
37	36.571932599939636	40.0	35.0	41.0	30.0	41.0
38	36.46027809289885	40.0	35.0	41.0	30.0	41.0
39	36.3850181613813	40.0	35.0	41.0	30.0	41.0
40	36.326599918820186	40.0	35.0	41.0	29.0	41.0
41	36.23879458384938	40.0	35.0	41.0	29.0	41.0
42	36.205288136298826	40.0	35.0	41.0	29.0	41.0
43	36.14383293610732	39.0	35.0	41.0	29.0	41.0
44	36.06795062602125	39.0	35.0	41.0	28.0	41.0
45	36.04435956412685	39.0	35.0	41.0	28.0	41.0
46	35.93104711551471	39.0	35.0	41.0	27.0	41.0
47	35.870173703985095	39.0	35.0	41.0	27.0	41.0
48	35.816285919465464	39.0	35.0	41.0	27.0	41.0
49	35.78148163566916	39.0	35.0	41.0	27.0	41.0
50	35.68199057065246	39.0	35.0	41.0	27.0	41.0
51	34.753783707835936	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	16.0
9	36.0
10	35.0
11	29.0
12	15.0
13	16.0
14	24.0
15	38.0
16	70.0
17	133.0
18	257.0
19	459.0
20	949.0
21	1462.0
22	2298.0
23	3742.0
24	6365.0
25	10319.0
26	14218.0
27	14993.0
28	13724.0
29	12838.0
30	13608.0
31	15979.0
32	20197.0
33	28409.0
34	47731.0
35	66344.0
36	67394.0
37	105142.0
38	181780.0
39	331935.0
40	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.55038872641362	19.17935534902116	23.794635887722073	17.475620036843146
2	36.668297201377975	22.867208559266466	24.262044274221246	16.20244996513431
3	30.110217208038883	22.44039007941051	31.213117825213615	16.23627488733699
4	26.63374374238939	24.648064694066587	31.13797445958182	17.5802171039622
5	21.48153159247734	35.634295348813005	26.47044742566323	16.41372563304643
6	23.42911857456574	33.40715839430493	29.276771124964874	13.886951906164462
7	76.39717744033804	5.434155886056847	13.866552876159155	4.3021137974459585
8	78.73983951375372	4.701351956121271	12.806427775985346	3.7523807541396503
9	73.43796509268029	6.541844030681807	14.548359231081461	5.4718316455564455
10	36.0872370762778	27.186703162890417	22.356816502398967	14.369243258432812
11	25.975979101401915	24.906799329746157	31.17950105637834	17.93772051247359
12	23.587419210474277	22.660304112069774	34.98464868915417	18.76762798830178
13	22.526877803565668	23.34231862035948	36.2192063112101	17.911597264864753
14	19.764474464785653	26.577021949772593	34.50214918351841	19.156354401923338
15	19.097759228999927	25.87471248816128	36.96023229915802	18.067295983680776
16	21.17731544602063	24.59498558537931	35.33663603342943	18.891062935170634
17	21.2669254706868	24.392660512265437	33.63664748186464	20.703766535183124
18	22.324969037186598	24.76889772384293	33.75810497174318	19.148028267227293
19	21.947691058772104	26.825452993765808	32.20413600741026	19.02271994005183
20	23.11678444678039	25.66676727412758	32.56580248326967	18.650645795822363
21	22.270745084978614	26.32588491200316	33.120843437444705	18.282526565573516
22	21.536067774736427	24.40098664696148	32.69673095136497	21.366214626937126
23	20.194415245152626	26.373864263189116	32.8550315872735	20.576688904384753
24	20.048395657920757	25.70777348750559	33.99706503751965	20.246765817054005
25	20.36489285305413	26.53664019649678	32.28125683003237	20.817210120416725
26	19.579217967798673	27.42555915198318	32.80340955215803	20.191813328060114
27	20.183487193364073	26.512702559245653	32.91695721407533	20.386853033314946
28	19.21609441836745	26.63707419626781	33.744887232913214	20.401944152451527
29	20.102515533445043	26.06558912606809	33.17923045700072	20.652664883486153
30	21.243195986803077	26.011261097176398	33.1389527804086	19.606590135611917
31	21.505989613146966	26.119604924908675	31.602780928988476	20.771624532955883
32	21.56135840887566	26.77351872859923	31.949772592446113	19.715350270078993
33	22.039590770479688	25.916239084957798	31.825817262158758	20.218352882403757
34	20.669317152878243	26.38104555436446	31.419605965675512	21.530031327081794
35	20.339810372282297	26.454627769740746	31.91272129304872	21.29284056492824
36	21.889512192583496	27.278706951281706	31.075840679412593	19.75594017672221
37	20.668588616092336	27.18722354630892	32.27293069533632	19.87125714226242
38	21.194696252198618	26.710136028225595	31.63306724394534	20.462100475630447
39	21.80531415546975	25.906351800006245	30.823350644755052	21.46498339976895
40	21.398790628935398	25.79217967798674	31.706753536005326	21.10227615707253
41	19.75979101401913	26.467429201835913	31.900232091004654	21.872547693140305
42	20.617174734344264	26.72803721782209	31.792616800058283	20.86217124777536
43	20.851867656089006	25.89021991403266	32.20861130480938	21.04930112506895
44	21.185017120614468	26.278321867552012	30.662864398488804	21.873796613344712
45	21.291487568040132	25.49295921234766	30.492074560536203	22.723478659076008
46	21.23944922618986	26.388851305641996	30.37217822091317	21.99952124725498
47	20.490513410280695	25.929977207206267	32.23442232236712	21.345087060145914
48	20.563887472289583	25.501493500411105	31.991923649344837	21.942695377954475
49	20.685240885484426	24.893789744283588	32.129513025196964	22.291456345035023
50	19.768117148715174	25.33684418679683	32.20163816700145	22.69340049748655
51	20.10220330339394	24.547214387560754	30.902657077734872	24.44792523131043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	579.0
1	957.5
2	1336.0
3	10348.5
4	19361.0
5	13077.0
6	6793.0
7	6626.0
8	6459.0
9	6374.5
10	6290.0
11	6231.5
12	6173.0
13	6053.5
14	5934.0
15	5657.5
16	5381.0
17	5249.0
18	5117.0
19	4981.0
20	4845.0
21	5291.5
22	5738.0
23	5691.0
24	5644.0
25	6351.5
26	8702.5
27	10346.0
28	12027.0
29	13708.0
30	15932.5
31	18157.0
32	21381.0
33	24605.0
34	27710.0
35	30815.0
36	33042.5
37	35270.0
38	39269.0
39	43268.0
40	46624.0
41	49980.0
42	56859.0
43	63738.0
44	73258.0
45	82778.0
46	85208.5
47	87639.0
48	86292.0
49	84945.0
50	76901.0
51	68857.0
52	61937.0
53	55017.0
54	50266.0
55	45515.0
56	41373.5
57	37232.0
58	34740.5
59	32249.0
60	30353.5
61	28458.0
62	25082.5
63	21707.0
64	17867.5
65	14028.0
66	11716.0
67	9404.0
68	7688.0
69	5972.0
70	4987.0
71	4002.0
72	3361.5
73	2721.0
74	2215.5
75	1359.5
76	1009.0
77	753.5
78	498.0
79	381.0
80	264.0
81	201.0
82	138.0
83	101.0
84	64.0
85	39.0
86	14.0
87	10.5
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	960830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.73653228184832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95020035421501	26.784602544894042
2	9.59431258079739	6.857349225316186
3	3.7607039494100105	4.031835542716959
4	2.04077263556391	2.917205486829694
5	1.3746001534795251	2.456172137972736
6	0.9654803853551367	2.0701752575241117
7	0.7322218050070588	1.8316947720475607
8	0.570003854813628	1.6295968926660163
9	0.48236119944784894	1.5514124918021217
>10	5.29814287286041	41.59565568353942
>50	0.20047123583930085	4.350986985948229
>100	0.026339119895051593	1.5658529554710234
>500	0.0026339119894046863	0.6828382277601908
>1k	0.001463284438558159	0.5981240350069736
>5k	0.0	0.0
>10k+	2.926568877116318E-4	1.0764977605047699
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10293	1.071261305329767	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1244	0.1294713945234849	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.12499609712436123	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1132	0.11781480594902323	TruSeq Adapter, Index 19 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	1096	0.1140680453358034	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	1046	0.1088642111507759	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	988	0.10282776349614395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0815336740110114E-4	0.0	0.0	0.10157884329173736	0.0
2	2.0815336740110114E-4	0.0	0.0	0.4667839263969693	0.0
3	2.0815336740110114E-4	0.0	0.0	0.704911378703829	0.0
4	2.0815336740110114E-4	0.0	0.0	1.0005932370970931	0.0
5	2.0815336740110114E-4	0.0	0.0	1.8091649927666704	0.0
6	2.0815336740110114E-4	0.0	0.0	2.672585160746438	0.0
7	2.0815336740110114E-4	0.0	0.0	3.3938365787912534	0.0
8	2.0815336740110114E-4	0.0	0.0	4.394117585837245	0.0
9	2.0815336740110114E-4	0.0	0.0	4.88275761581133	0.0
10	2.0815336740110114E-4	0.0	0.0	5.85826837213659	0.0
11	2.0815336740110114E-4	0.0	0.0	6.969182893956267	0.0
12	2.0815336740110114E-4	0.0	0.0	7.917009252417181	0.0
13	2.0815336740110114E-4	0.0	0.0	8.339352434874016	0.0
14	2.0815336740110114E-4	0.0	0.0	8.539908204364977	0.0
15	2.0815336740110114E-4	0.0	0.0	8.790733012083303	0.0
16	3.122300511016517E-4	0.0	0.0	9.286866563283828	0.0
17	3.122300511016517E-4	0.0	0.0	9.920173183601678	0.0
18	3.122300511016517E-4	0.0	0.0	10.57856228469136	0.0
19	3.122300511016517E-4	0.0	0.0	11.017349583172882	0.0
20	3.122300511016517E-4	0.0	0.0	11.414506208174183	0.0
21	3.122300511016517E-4	0.0	0.0	11.942487224587076	0.0
22	3.122300511016517E-4	0.0	0.0	12.510745917592082	0.0
23	3.122300511016517E-4	0.0	0.0	13.100340330755701	0.0
24	3.122300511016517E-4	0.0	0.0	13.572224014653997	0.0
25	3.122300511016517E-4	0.0	0.0	13.97926792460685	0.0
26	3.122300511016517E-4	0.0	0.0	14.37423893925044	0.0
27	3.122300511016517E-4	0.0	0.0	14.7827399227751	0.0
28	4.163067348022023E-4	0.0	0.0	15.208309482426651	0.0
29	5.203834185027529E-4	0.0	0.0	15.66343682024916	0.0
30	6.244601022033034E-4	0.0	0.0	16.183195778649708	0.0
31	7.285367859038539E-4	0.0	0.0	16.644255487443147	0.0
32	8.326134696044046E-4	0.0	0.0	17.092409687457717	0.0
33	8.326134696044046E-4	0.0	0.0	17.52953175900003	0.0
34	8.326134696044046E-4	0.0	0.0	17.958744002581103	0.0
35	8.326134696044046E-4	0.0	0.0	18.40096583162474	0.0
36	8.326134696044046E-4	0.0	0.0	18.82039486693796	0.0
37	8.326134696044046E-4	0.0	0.0	19.26136777577719	0.0
38	8.326134696044046E-4	0.0	0.0	19.6975531571662	0.0
39	8.326134696044046E-4	0.0	0.0	20.156219102234527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	40	6.8139343E-9	45.000004	10
TATTACG	25	3.8903265E-5	45.0	1
TCGACAG	30	2.1651285E-6	44.999996	1
CGTTTTT	6385	0.0	42.991383	1
CGTTATT	775	0.0	42.967743	1
TATAGCG	50	1.0804797E-9	40.5	1
ATGTACG	40	3.457426E-7	39.375004	1
AGCGAAG	75	0.0	39.0	1
CTTACGG	75	0.0	39.0	2
CTAACGG	35	6.247219E-6	38.571426	2
TCGGAAT	35	6.247219E-6	38.571426	12
CGACAGG	100	0.0	38.25	2
CACGACC	65	9.094947E-12	38.07692	27
GTAGACG	65	9.094947E-12	38.07692	1
GCGATTC	65	9.094947E-12	38.07692	9
TACGGGC	180	0.0	37.5	4
CGTAAGG	150	0.0	37.5	2
TACGCGG	60	1.546141E-10	37.499996	2
CAAGCGT	30	1.1397827E-4	37.499996	35
ATCGATT	30	1.1397827E-4	37.499996	11
>>END_MODULE