SRR2936003.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936003.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172645
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4002	2.3180514929479568	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	2611	1.5123519360537518	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2558	1.4816531031886242	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	1808	1.0472356569839845	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1278	0.7402473283327059	RNA PCR Primer, Index 35 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	755	0.4373135625126705	RNA PCR Primer, Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	558	0.32320657997625185	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCT	519	0.3006168727736106	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	477	0.27628949578615075	TruSeq Adapter, Index 10 (95% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.27223493295490747	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	382	0.2212632859335631	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	334	0.19346056937646616	RNA PCR Primer, Index 35 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTC	315	0.18245532740594864	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	287	0.16623707608097543	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	276	0.15986562020330736	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	264	0.15291494106403314	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	253	0.1465434851863651	No Hit
GCTAAGAGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	197	0.11410698253641867	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	189	0.10947319644356918	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	35	1.2039163E-7	45.000004	31
CGGCGAG	35	1.2039163E-7	45.000004	31
ACGGGCA	35	1.2039163E-7	45.000004	5
TTGCGAG	35	1.2039163E-7	45.000004	1
GGCGATT	35	1.2039163E-7	45.000004	8
ACAACCA	20	7.016619E-4	45.0	24
GGTACCT	40	6.7575456E-9	45.0	8
ACACGGG	20	7.016619E-4	45.0	3
CGTATGG	20	7.016619E-4	45.0	2
CAAGTCG	20	7.016619E-4	45.0	1
CCGATTA	20	7.016619E-4	45.0	18
GCTGGTC	20	7.016619E-4	45.0	35
TAGACGC	20	7.016619E-4	45.0	37
TGTATAG	20	7.016619E-4	45.0	33
ATAGACG	20	7.016619E-4	45.0	36
TACGGGT	20	7.016619E-4	45.0	4
GGATACG	20	7.016619E-4	45.0	1
TGTGCGG	20	7.016619E-4	45.0	2
GCCATAT	20	7.016619E-4	45.0	45
GGAACTT	20	7.016619E-4	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content