##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936003.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172645
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873167482406092	33.0	31.0	34.0	30.0	34.0
2	32.02718873989979	33.0	31.0	34.0	30.0	34.0
3	31.864791914043266	33.0	31.0	34.0	30.0	34.0
4	35.6277332097657	37.0	35.0	37.0	33.0	37.0
5	32.32756813113615	37.0	35.0	37.0	19.0	37.0
6	33.95257319933969	37.0	35.0	37.0	19.0	37.0
7	35.45239074401228	37.0	35.0	37.0	32.0	37.0
8	35.640076457470535	37.0	35.0	37.0	33.0	37.0
9	37.590118451157	39.0	37.0	39.0	35.0	39.0
10	37.34407020185931	39.0	37.0	39.0	34.0	39.0
11	37.2573025572707	39.0	37.0	39.0	34.0	39.0
12	36.93115352312549	39.0	35.0	39.0	33.0	39.0
13	36.61023487503258	39.0	35.0	39.0	33.0	39.0
14	37.71409539807119	40.0	36.0	41.0	33.0	41.0
15	37.85840308146775	40.0	36.0	41.0	33.0	41.0
16	38.018089142459964	40.0	36.0	41.0	34.0	41.0
17	37.95620492918996	40.0	36.0	41.0	33.0	41.0
18	37.77834284224854	39.0	36.0	41.0	33.0	41.0
19	37.50726635581685	39.0	36.0	41.0	33.0	41.0
20	37.21717396970662	39.0	35.0	41.0	33.0	41.0
21	37.23186886385357	39.0	35.0	41.0	33.0	41.0
22	37.22535839439312	39.0	35.0	41.0	33.0	41.0
23	37.198227576819484	39.0	35.0	41.0	33.0	41.0
24	37.149381679168236	39.0	35.0	41.0	33.0	41.0
25	36.99965825827565	38.0	35.0	41.0	33.0	41.0
26	36.96196240841032	38.0	35.0	41.0	33.0	41.0
27	36.881282400301195	38.0	35.0	41.0	33.0	41.0
28	36.74358365431956	38.0	35.0	41.0	33.0	41.0
29	36.73330823365866	38.0	35.0	41.0	33.0	41.0
30	36.5192562773321	38.0	35.0	41.0	32.0	41.0
31	36.24098583799125	38.0	35.0	40.0	31.0	41.0
32	35.85129601204785	38.0	35.0	40.0	30.0	41.0
33	35.47208433490689	38.0	35.0	40.0	27.0	41.0
34	35.03897014104086	38.0	35.0	41.0	24.0	41.0
35	34.938805062411305	38.0	35.0	41.0	23.0	41.0
36	34.62102001216369	38.0	35.0	40.0	21.0	41.0
37	34.502962726983114	38.0	35.0	40.0	21.0	41.0
38	34.31380578644038	38.0	34.0	40.0	20.0	41.0
39	34.335984245127285	38.0	34.0	40.0	20.0	41.0
40	34.3152538445944	38.0	34.0	40.0	20.0	41.0
41	34.14747603463755	37.0	34.0	40.0	18.0	41.0
42	34.129201540733874	37.0	34.0	40.0	18.0	41.0
43	33.98459845347389	37.0	34.0	40.0	18.0	41.0
44	33.9211677140954	37.0	34.0	40.0	18.0	41.0
45	33.85100640041704	37.0	34.0	40.0	18.0	41.0
46	33.709195169278	37.0	34.0	40.0	18.0	41.0
47	33.52311969648701	37.0	33.0	40.0	17.0	41.0
48	33.4933649975383	36.0	33.0	40.0	17.0	41.0
49	33.53264791914043	36.0	33.0	40.0	17.0	41.0
50	33.38317356425034	36.0	33.0	40.0	15.0	41.0
51	32.34131309913406	35.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	6.0
11	1.0
12	2.0
13	4.0
14	9.0
15	5.0
16	13.0
17	27.0
18	83.0
19	149.0
20	386.0
21	615.0
22	987.0
23	1449.0
24	1936.0
25	2763.0
26	3780.0
27	3998.0
28	3850.0
29	3606.0
30	3594.0
31	4319.0
32	5350.0
33	7609.0
34	13246.0
35	19347.0
36	12074.0
37	16159.0
38	27041.0
39	40185.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.20130904457123	15.73691679457847	21.33510961800226	21.72666454284804
2	36.83338642879898	27.29531697992991	21.235483217005996	14.63581337426511
3	24.234122042341223	26.696979350690725	35.58110573720641	13.48779286976165
4	23.77074343305627	20.603550638593646	40.59833762923919	15.027368299110893
5	24.260766312375104	31.334240783109852	29.79061079092936	14.61438211358568
6	20.84566596194503	33.1645862897854	32.62996321932289	13.359784528946683
7	64.46986590981494	5.317269541544788	25.598192823423787	4.614671725216485
8	64.36618494598743	10.54765559384865	19.31477888151988	5.771380578644038
9	60.50855802369023	6.132815893886299	20.874047901763735	12.484578180659735
10	40.344058617394076	21.26038981725506	26.40099626400996	11.994555301340903
11	33.021518144168674	19.659416722175564	32.315444988270734	15.003620145385039
12	28.236554780039967	17.022213212082598	36.56404761215211	18.177184395725334
13	22.00005792232616	25.073416548408584	38.72455037794318	14.201975151322078
14	15.725332329346347	31.763445219960033	34.64739783949724	17.863824611196385
15	11.600683483448696	22.403197312404068	49.94062961568537	16.05548958846187
16	14.159691853224826	20.180138434359524	42.90827999652466	22.75188971589099
17	14.66014075125257	21.64499406296157	38.67705407049147	25.017811115294393
18	16.502649946421847	21.09936575052854	40.39850560398506	21.999478699064554
19	18.51776767354977	24.268296214775987	36.26864374873295	20.945292362941295
20	22.317472269686352	23.1556083292305	38.094934692577255	16.431984708505894
21	18.707173680094993	27.857163543687914	36.24895015783834	17.186712618378756
22	15.089924411364361	22.714819427148196	37.94839120739089	24.246864954096555
23	16.477164122911176	26.337281705233284	35.52723797387703	21.65831619797851
24	18.786527266935042	22.063193257841235	37.258536302817916	21.891743172405803
25	15.139158388600887	26.743896434880828	36.40128587564077	21.715659300877522
26	15.816849604680124	24.98769150569087	38.348055257899155	20.84740363172985
27	19.75093399750934	26.12934055431666	35.37374380955139	18.74598163862261
28	14.214138839815806	23.91554924845782	43.298097251585624	18.57221466014075
29	18.27796924324481	20.13495901995424	41.99542413623331	19.591647600567637
30	19.636247791711313	24.924556170175794	38.65967737264329	16.779518665469606
31	23.460858988096962	21.80254279011845	33.633177908424805	21.103420313359784
32	24.71545657273596	24.95004199368647	34.04616409395002	16.28833733962756
33	22.580439630455558	21.668162993425817	33.12056532190333	22.630832054215297
34	23.10695357525558	22.12632859335631	34.2228271887399	20.543890642648208
35	20.876944018071768	21.795592110979175	34.91673665614411	22.410727214804947
36	25.02128645486403	21.20304671435605	34.28538330099337	19.490283529786556
37	21.446899707492253	22.615193026151932	38.32430710417331	17.613600162182514
38	16.935908946103275	23.85473080598917	36.67062469228764	22.538735555619912
39	21.345535636711173	22.861942135596166	36.70943265081525	19.083089576877406
40	22.1066350024617	20.08224970314808	37.534246575342465	20.27686871904776
41	20.214312606794287	21.55695212719743	31.37826175099192	26.850473515016365
42	21.20130904457123	19.458426250398215	35.757189608734684	23.583075096295865
43	22.72350777607229	20.75530713313447	32.00440209678821	24.51678299400504
44	20.388658808537752	23.46838889049784	34.39717339048336	21.745778910481047
45	18.990993078282024	23.171826580555475	33.1362043499667	24.700975991195804
46	24.15361000897796	24.440904746734628	32.07217121839613	19.33331402589128
47	18.420458165599932	22.943033392221032	38.298242057401026	20.338266384778013
48	21.346694083234382	21.032175852182224	34.06238234527499	23.558747719308407
49	19.30609053259579	17.585797445625417	39.59280604709085	23.515305974687944
50	19.628138666048827	20.599496075762403	35.0841321787483	24.68823307944047
51	19.06165831619798	21.869153465203162	31.033044687074636	28.036143531524228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	352.5
2	350.0
3	3362.0
4	6374.0
5	4058.0
6	1742.0
7	1795.0
8	1848.0
9	1885.0
10	1922.0
11	1956.0
12	1990.0
13	1918.5
14	1847.0
15	1659.5
16	1472.0
17	1408.5
18	1345.0
19	1254.5
20	1164.0
21	1066.5
22	969.0
23	1037.0
24	1105.0
25	1061.0
26	1185.0
27	1353.0
28	1459.5
29	1566.0
30	1834.0
31	2102.0
32	2465.0
33	2828.0
34	2971.0
35	3114.0
36	3668.0
37	4222.0
38	4950.5
39	5679.0
40	5973.0
41	6267.0
42	8243.0
43	10219.0
44	11889.0
45	13559.0
46	18820.5
47	24082.0
48	20871.0
49	17660.0
50	17587.0
51	17514.0
52	14285.0
53	11056.0
54	9265.5
55	7475.0
56	6452.0
57	5429.0
58	4922.5
59	4416.0
60	4193.5
61	3971.0
62	3351.5
63	2732.0
64	2150.5
65	1569.0
66	1269.5
67	970.0
68	739.5
69	509.0
70	405.5
71	302.0
72	296.0
73	290.0
74	212.5
75	81.5
76	28.0
77	41.5
78	55.0
79	45.5
80	36.0
81	21.0
82	6.0
83	3.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	172645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.60728662863101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64697279906405	30.75385907498045
2	9.75723895875987	7.729155202872947
3	3.664814273179292	4.354600480755307
4	1.7768353319684116	2.8150250514060646
5	1.0821877742029833	2.1431260679428887
6	0.6595495758993858	1.5673781459063396
7	0.5352442234571512	1.4839699962350488
8	0.36999122550453345	1.1723478814909207
9	0.377303305059959	1.3449564134495642
>10	4.023106171395145	34.04558487068841
>50	0.06434630008774495	1.69944104955255
>100	0.030710734132787368	2.729879231949955
>500	0.004387247733255338	1.061137015262533
>1k	0.007312079555425563	7.099539517507022
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4002	2.3180514929479568	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	2611	1.5123519360537518	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2558	1.4816531031886242	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	1808	1.0472356569839845	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1278	0.7402473283327059	RNA PCR Primer, Index 35 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	755	0.4373135625126705	RNA PCR Primer, Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	558	0.32320657997625185	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCT	519	0.3006168727736106	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	477	0.27628949578615075	TruSeq Adapter, Index 10 (95% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.27223493295490747	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	382	0.2212632859335631	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	334	0.19346056937646616	RNA PCR Primer, Index 35 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTC	315	0.18245532740594864	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	287	0.16623707608097543	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	276	0.15986562020330736	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	264	0.15291494106403314	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	253	0.1465434851863651	No Hit
GCTAAGAGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	197	0.11410698253641867	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	189	0.10947319644356918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011584465232123721	0.0	0.0	0.3006168727736106	0.0
2	0.0011584465232123721	0.0	0.0	1.8685742419415563	0.0
3	0.0011584465232123721	0.0	0.0	2.3018332416229836	0.0
4	0.0011584465232123721	0.0	0.0	2.9580931970227926	0.0
5	0.0011584465232123721	0.0	0.0	5.284833038894842	0.0
6	0.0011584465232123721	0.0	0.0	6.0644675490167685	0.0
7	0.0011584465232123721	0.0	0.0	7.289524747313852	0.0
8	0.0011584465232123721	0.0	0.0	8.83605085580237	0.0
9	0.0011584465232123721	0.0	0.0	9.447710620058501	0.0
10	0.0011584465232123721	0.0	0.0	12.464884589765125	0.0
11	0.0011584465232123721	0.0	0.0	14.410495525500304	0.0
12	0.0011584465232123721	0.0	0.0	17.614758608705724	0.0
13	0.0011584465232123721	0.0	0.0	18.30982652263315	0.0
14	0.0011584465232123721	0.0	0.0	18.58901213472733	0.0
15	0.0011584465232123721	0.0	0.0	19.259752671667293	0.0
16	0.0011584465232123721	0.0	0.0	20.157548727156883	0.0
17	0.0011584465232123721	0.0	0.0	21.196096035216776	0.0
18	0.0011584465232123721	0.0	0.0	22.36149323756842	0.0
19	0.0011584465232123721	0.0	0.0	23.44753685308002	0.0
20	0.0011584465232123721	0.0	0.0	24.06556807321382	0.0
21	0.0011584465232123721	0.0	0.0	24.758898317356426	0.0
22	0.0011584465232123721	0.0	0.0	25.483506617625764	0.0
23	0.0011584465232123721	0.0	0.0	26.262561904486084	0.0
24	0.0011584465232123721	0.0	0.0	26.850473515016365	0.0
25	0.0011584465232123721	0.0	0.0	27.299371542761158	0.0
26	0.0017376697848185583	0.0	0.0	27.74711112398274	0.0
27	0.0017376697848185583	0.0	0.0	28.21512351936054	0.0
28	0.0017376697848185583	0.0	0.0	28.63216426771699	0.0
29	0.0017376697848185583	0.0	0.0	29.09554287700194	0.0
30	0.0017376697848185583	0.0	0.0	29.617423035709113	0.0
31	0.0017376697848185583	0.0	0.0	30.124243389614527	0.0
32	0.0017376697848185583	0.0	0.0	30.544180254279013	0.0
33	0.0017376697848185583	0.0	0.0	30.931101393031945	0.0
34	0.0017376697848185583	0.0	0.0	31.366677285759796	0.0
35	0.0017376697848185583	0.0	0.0	31.830055895044744	0.0
36	0.0017376697848185583	0.0	0.0	32.25115120623244	0.0
37	0.0017376697848185583	0.0	0.0	32.682672536129054	0.0
38	0.0017376697848185583	0.0	0.0	33.08233658663732	0.0
39	0.0017376697848185583	0.0	0.0	33.53818529352139	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	35	1.2039163E-7	45.000004	31
CGGCGAG	35	1.2039163E-7	45.000004	31
ACGGGCA	35	1.2039163E-7	45.000004	5
TTGCGAG	35	1.2039163E-7	45.000004	1
GGCGATT	35	1.2039163E-7	45.000004	8
ACAACCA	20	7.016619E-4	45.0	24
GGTACCT	40	6.7575456E-9	45.0	8
ACACGGG	20	7.016619E-4	45.0	3
CGTATGG	20	7.016619E-4	45.0	2
CAAGTCG	20	7.016619E-4	45.0	1
CCGATTA	20	7.016619E-4	45.0	18
GCTGGTC	20	7.016619E-4	45.0	35
TAGACGC	20	7.016619E-4	45.0	37
TGTATAG	20	7.016619E-4	45.0	33
ATAGACG	20	7.016619E-4	45.0	36
TACGGGT	20	7.016619E-4	45.0	4
GGATACG	20	7.016619E-4	45.0	1
TGTGCGG	20	7.016619E-4	45.0	2
GCCATAT	20	7.016619E-4	45.0	45
GGAACTT	20	7.016619E-4	45.0	8
>>END_MODULE